Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,197,628	C→T	R242R (CGC→CGT)	nudC →	RNA decapping hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,197,628	0	C	T	98.1%	176.4 / NA	53	R242R (CGC→CGT)	nudC	RNA decapping hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (28/24);  minor base G (0/1);  total (28/25)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCTGCGTCGCTA  >  NC_000913/4197337‑4197915                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                gCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGAACCGAAAGGATTGCTCTAGGAGAAATGGTATCGCTATGACGATTTGGCGTTACTCCCGACGCCAGGAACAGTAGCGCGTCGTCTGAt                                                                                                                                                                                                                                                                                         >  2:74711/1‑300 (MQ=255)   aTACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATag                                                                                                                                                                                                                                                                                       <  1:179768/300‑1 (MQ=255)        cGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGAt                                                                                                                                                                                                                                                                                  <  1:335451/300‑1 (MQ=255)           cATCGTAACGGTGTCCATACAGTACTTGCCGNATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGANTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTCCTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACg                                                                                                                                                                                                                                                                               >  2:496128/1‑300 (MQ=255)             tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCTAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTGCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGt                                                                                                                                                                                                                                                                             <  1:447718/300‑1 (MQ=255)             tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTACAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCACGGCACCGTAGCGCGTCGTCTGCTAGAAGATACGGt                                                                                                                                                                                                                                                                             >  2:180751/1‑300 (MQ=255)             tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGt                                                                                                                                                                                                                                                                             >  2:406704/1‑300 (MQ=255)             tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGACGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGt                                                                                                                                                                                                                                                                             >  2:427985/1‑300 (MQ=255)                     gtgtCCATACAGTACTTGCCGGATTCGTCGANGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCNTGGCCGTTTCCTCAGTCTTTAATGACGGCGTTTATGGCGGAATATGAAAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTCCTCCCGCCGCCCAGCACCGTAGCGCGTCGTCTGGTAGAAGATACGGTGGCGAtgt                                                                                                                                                                                                                                                                     >  2:480508/1‑300 (MQ=255)                      tgtCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTAAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATNTGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGACAACCGGCACCGTCGCGCGTCGTCTGATAGAAGATACGGTGGCGAtgtg                                                                                                                                                                                                                                                                    >  2:158125/1‑300 (MQ=255)                           aTACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTAAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAAATGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCggg                                                                                                                                                                                                                                                               >  2:159/1‑300 (MQ=255)                                gTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCagag                                                                                                                                                                                                                                                          >  1:153419/1‑300 (MQ=255)                                      gCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAg                                                                                                                                                                                                                                                    <  1:145418/300‑1 (MQ=255)                                      gCCGGAGTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAg                                                                                                                                                                                                                                                    <  2:238879/300‑1 (MQ=255)                                                 cgAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCATTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                                                                                                                                                                         <  1:370184/300‑1 (MQ=255)                                                 cgAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                                                                                                                                                                         <  1:61817/300‑1 (MQ=255)                                                 cgAAGTGGGCGAAACCCGCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGNGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGNCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                                                                                                                                                                         <  2:634001/300‑1 (MQ=255)                                                 cgAAGGGGGCGAAACCCTCGAGCAGGCATTCGCGCGGGAAGTGAGGGAAGAGAGCGGAATTAAAGTGAAAAACTTGCGTTACGTGACTTCTCAGTCGTTGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGAGAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                                                                                                                                                                         <  1:74967/300‑1 (MQ=255)                                                 cgAAGGGGGCGAAACCCTCGAGCAGGCAGGCGAGCGGTAAGTGATGGAGGAGAGCGGAATTAAAGGGAAAAACTGGCGTGACGTGACGGCTCAGTCGGGGATGTGTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAACATGACAGCGGCGACATCGTGATCGACCCGAAAGAATGGCTCGAGGCGAACTGGTATCGCTATTATGAGTGGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGGCGTCTGATAGAAGAGACGGTGGCGAGGTGGCGGGCAGAGTATGAGTGATGAGacaa                                                                                                                                                                                                                                         <  1:553790/300‑2 (MQ=255)                                                                 cTCGAGCAGGCAGTCGCGCGGGAAGTGATGGNAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATNTGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCAc                                                                                                                                                                                                                         >  2:496641/1‑300 (MQ=255)                                                                       caggcagTCGCGCGGGAAGTGATGTAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGa                                                                                                                                                                                                                   <  2:259896/300‑1 (MQ=255)                                                                       caggcagTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGa                                                                                                                                                                                                                   <  2:532377/300‑1 (MQ=255)                                                                                         gTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGNCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGaa                                                                                                                                                                                                 >  1:491043/1‑300 (MQ=255)                                                                                          tGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAAc                                                                                                                                                                                                >  1:648937/1‑300 (MQ=255)                                                                                          tGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGCTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATNAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGNACGAAc                                                                                                                                                                                                >  2:573414/1‑300 (MQ=255)                                                                                                            ttAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCTGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATc                                                                                                                                                                              <  1:636567/300‑1 (MQ=255)                                                                                                                               ttACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCa                                                                                                                                                           >  1:554110/1‑300 (MQ=255)                                                                                                                                  cGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGcc                                                                                                                                                        <  2:380522/300‑1 (MQ=255)                                                                                                                                                        tttCCTCAGTCTTTAATGACCGCGTTTATGTCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCTTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAAAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATgg                                                                                                                                  <  2:680701/300‑1 (MQ=255)                                                                                                                                                          tCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCTAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGgat                                                                                                                                <  2:581909/300‑1 (MQ=255)                                                                                                                                                          tCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGgat                                                                                                                                <  1:521479/300‑1 (MQ=255)                                                                                                                                                          tCCTCAGTCTGGAATGACCGCGTTTATTGCGGAATAGGACAGCGGCGACACCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTGACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCAAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGgat                                                                                                                                <  2:71599/300‑1 (MQ=255)                                                                                                                                                                gTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGTGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATgcg                                                                                                                          >  1:9365/1‑300 (MQ=255)                                                                                                                                                                gTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCACGTTGATGTCACTCCAGTATGGATGATgcg                                                                                                                          >  2:661488/1‑300 (MQ=255)                                                                                                                                                                      aaTGACCGCGTTTATTGCGGAATATGACAGCGGCGACATCGTGATCGACCCTAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGc                                                                                                                    <  1:120907/300‑1 (MQ=255)                                                                                                                                                                      aaTGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGGCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGc                                                                                                                    <  1:121073/300‑1 (MQ=255)                                                                                                                                                                                 ctgcgagatgtgtataagagacagACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATc                                                                                                         >  1:450231/24‑300 (MQ=255)                                                                                                                                                                                     ggCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAcc                                                                                                     <  2:439919/300‑1 (MQ=255)                                                                                                                                                                                       cGGAATCTGACAGCGGCGACATCGTGATCGACCCGAAAGACTTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCgg                                                                                                   <  2:586299/300‑1 (MQ=255)                                                                                                                                                                                         gAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGaa                                                                                                 >  1:382791/1‑300 (MQ=255)                                                                                                                                                                                                        gACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCAcgcgc                                                                                  <  1:305040/300‑1 (MQ=255)                                                                                                                                                                                                          cATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACgcgcgc                                                                                >  1:640515/1‑300 (MQ=255)                                                                                                                                                                                                          cATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCACCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACgcgcgc                                                                                >  2:50344/1‑300 (MQ=255)                                                                                                                                                                                                              gTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCcag                                                                            >  1:573733/1‑300 (MQ=255)                                                                                                                                                                                                                     aCCCGAAAGAACTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGCCGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACACGCGCCCAAGCAGGCg                                                                     >  1:139480/1‑300 (MQ=255)                                                                                                                                                                                                                                  gCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCt                                                        >  1:103383/1‑300 (MQ=255)                                                                                                                                                                                                                                                ggTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCg                                          >  1:615043/1‑300 (MQ=255)                                                                                                                                                                                                                                                ggTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCAAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCACTATCTACCGGAATATAAAGCCACGCGCGCACAGGCAGGCGATTTTATGTCACTGTGCaaaaaagcag                                          >  2:219540/1‑295 (MQ=255)                                                                                                                                                                                                                                                     cGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTg                                     >  2:405076/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAANGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGAc                       <  1:506863/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ccGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACt                      >  2:444756/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         aCTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCa                 <  1:676610/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                ccgccCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCATGACGCACTAAGGAACAGCCAAAATGAACGAACTTAAAAACGATTGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTCGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTAACGGAATATAAAGCCACGCGCGACCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCTg          >  2:131579/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        cACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCTGCGTCGCTa  <  2:385153/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                GCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCCCGTTGATGTCACTCCAGTATGGATGATGCGCCAGGCGGGTCGCTATCTACCGGAATATAAAGCCACGCGCGCCCAGGCAGGCGATTTTATGTCGCTGTGCAAAAACGCCGAGCTGGCGTGCGAAGTGACTTTGCAACCGCTGCGTCGCTA  >  NC_000913/4197337‑4197915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑