Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,197,479	C→T	P193S (CCG→TCG)	nudC →	RNA decapping hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,197,479	0	C	T	100.0%	186.4 / NA	54	P193S (CCG→TCG)	nudC	RNA decapping hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/29);  total (25/29)

CGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCC  >  NC_000913/4197183‑4197765                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                               cGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGt                                                                                                                                                                                                                                                                                             >  1:390746/1‑300 (MQ=255)   cATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATNGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTAAGTGACTTCTCAGTCGTgg                                                                                                                                                                                                                                                                                           >  2:511200/1‑300 (MQ=255)   cATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTgg                                                                                                                                                                                                                                                                                           >  2:660800/1‑300 (MQ=255)     tAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGcc                                                                                                                                                                                                                                                                                         <  1:639869/300‑1 (MQ=255)                        gggCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATg                                                                                                                                                                                                                                                                      <  1:384078/300‑1 (MQ=255)                                     aTCCGAGCAAAACCGAATGTTCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATgg                                                                                                                                                                                                                                                         <  2:414336/300‑1 (MQ=255)                                                aCCGAATGTGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGAc                                                                                                                                                                                                                                              <  2:381597/300‑1 (MQ=255)                                                aCCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGAc                                                                                                                                                                                                                                              <  1:648324/300‑1 (MQ=255)                                                aCCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGAc                                                                                                                                                                                                                                              <  2:614683/300‑1 (MQ=255)                                                             tGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGNAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCg                                                                                                                                                                                                                                 <  1:502430/300‑1 (MQ=255)                                                              gCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGt                                                                                                                                                                                                                                >  1:312213/1‑300 (MQ=255)                                                                                 gAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAgctgtctcttatacacatctacaagcccacga                                                                                                                                                                                                             >  1:170738/1‑270 (MQ=255)                                                                                     gTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCg                                                                                                                                                                                                         >  1:449065/1‑300 (MQ=255)                                                                                                   aTCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTAt                                                                                                                                                                                           <  1:276061/300‑1 (MQ=255)                                                                                                       cccccTGCATTATTTTTGCCATCCGTCGCGATGATTCGATCCTCCCCGCCCAGCATGCCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCt                                                                                                                                                                                       <  2:388514/300‑1 (MQ=255)                                                                                                       cccccTGCATTATTGTTGCCATCCGTCGCGAGGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCt                                                                                                                                                                                       <  2:401024/300‑1 (MQ=255)                                                                                                        ccccTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTNAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTa                                                                                                                                                                                      >  1:487309/1‑300 (MQ=255)                                                                                                        ccccTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTa                                                                                                                                                                                      <  1:588816/300‑1 (MQ=255)                                                                                                             gCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACg                                                                                                                                                                                 >  2:395977/1‑300 (MQ=255)                                                                                                                ttattGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGAtt                                                                                                                                                                              >  1:38719/1‑300 (MQ=255)                                                                                                                             ccGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCAGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTccc                                                                                                                                                                 >  2:45583/1‑300 (MQ=255)                                                                                                                                       gatTCGATCCTCCTCGCCCAGCACACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGCAGTGGGAGAAACCCTCGAGCAGGCAGTCGCGCGGGGAGTGATGGAAGAGAGCGGAATTAAAGTTAAAACCTTGCGTTACGTGACTTCTAAGTCGTGGCCGTTTCCTCCGTCTTTAATGACCGCGTTTATGGCGGAATATGGCAGCGGCGACATTGTGATCGACCCGGAAAAATTGCTCACGGCGAAATGGTAGCGCTATGACGATTTGCCGTTACTCCCGCCGCCCggt                                                                                                                                                       >  2:145389/1‑299 (MQ=255)                                                                                                                                         ttCGATCCTCTTCGCCCAGCATACCCGCTATCGTGACGGTTTCCATACAGTACGTGCCGGATTCGTCTAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGGGAGCGGAATTAAAATTAAAAACTTGCGCTACGTGACTTCTCAGTCGTTGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCTATGCGAACTGGTATCGCTATGACGATTGGCCGTTACTCCCGCCGCCCTGCAc                                                                                                                                                     <  2:264185/300‑1 (MQ=255)                                                                                                                                         ttCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGNTGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCAc                                                                                                                                                     <  1:488856/300‑1 (MQ=255)                                                                                                                                              tcctccTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAg                                                                                                                                                <  1:624547/300‑1 (MQ=255)                                                                                                                                                 tcctcGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAgcgc                                                                                                                                             <  1:381845/300‑1 (MQ=255)                                                                                                                                                           gCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGAACCGAAAGGATTGCTCTAGGAGAAATGGTATCGCTATGACGATTTGGCGTTACTCCCGACGCCAGGAACAGTAGCGCGTCGTCTGAt                                                                                                                                   >  2:74711/1‑300 (MQ=255)                                                                                                                                                             aTACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATag                                                                                                                                 <  1:179768/300‑1 (MQ=255)                                                                                                                                                                  cGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGAt                                                                                                                            <  1:335451/300‑1 (MQ=255)                                                                                                                                                                     cATCGTAACGGTGTCCATACAGTACTTGCCGNATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGANTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTCCTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACg                                                                                                                         >  2:496128/1‑300 (MQ=255)                                                                                                                                                                       tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCTAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTGCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGt                                                                                                                       <  1:447718/300‑1 (MQ=255)                                                                                                                                                                       tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTACAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCACGGCACCGTAGCGCGTCGTCTGCTAGAAGATACGGt                                                                                                                       >  2:180751/1‑300 (MQ=255)                                                                                                                                                                       tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGt                                                                                                                       >  2:406704/1‑300 (MQ=255)                                                                                                                                                                       tCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGACGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGt                                                                                                                       >  2:427985/1‑300 (MQ=255)                                                                                                                                                                               gtgtCCATACAGTACTTGCCGGATTCGTCGANGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCNTGGCCGTTTCCTCAGTCTTTAATGACGGCGTTTATGGCGGAATATGAAAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTCCTCCCGCCGCCCAGCACCGTAGCGCGTCGTCTGGTAGAAGATACGGTGGCGAtgt                                                                                                               >  2:480508/1‑300 (MQ=255)                                                                                                                                                                                tgtCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTAAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATNTGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGACAACCGGCACCGTCGCGCGTCGTCTGATAGAAGATACGGTGGCGAtgtg                                                                                                              >  2:158125/1‑300 (MQ=255)                                                                                                                                                                                     aTACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTAAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAAATGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCggg                                                                                                         >  2:159/1‑300 (MQ=255)                                                                                                                                                                                          gTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCagag                                                                                                    >  1:153419/1‑300 (MQ=255)                                                                                                                                                                                                gCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAg                                                                                              <  1:145418/300‑1 (MQ=255)                                                                                                                                                                                                gCCGGAGTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAg                                                                                              <  2:238879/300‑1 (MQ=255)                                                                                                                                                                                                           cgAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCATTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                   <  1:370184/300‑1 (MQ=255)                                                                                                                                                                                                           cgAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                   <  1:61817/300‑1 (MQ=255)                                                                                                                                                                                                           cgAAGTGGGCGAAACCCGCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGNGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGNCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                   <  2:634001/300‑1 (MQ=255)                                                                                                                                                                                                           cgAAGGGGGCGAAACCCTCGAGCAGGCATTCGCGCGGGAAGTGAGGGAAGAGAGCGGAATTAAAGTGAAAAACTTGCGTTACGTGACTTCTCAGTCGTTGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGAGAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATacac                                                                                   <  1:74967/300‑1 (MQ=255)                                                                                                                                                                                                           cgAAGGGGGCGAAACCCTCGAGCAGGCAGGCGAGCGGTAAGTGATGGAGGAGAGCGGAATTAAAGGGAAAAACTGGCGTGACGTGACGGCTCAGTCGGGGATGTGTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAACATGACAGCGGCGACATCGTGATCGACCCGAAAGAATGGCTCGAGGCGAACTGGTATCGCTATTATGAGTGGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGGCGTCTGATAGAAGAGACGGTGGCGAGGTGGCGGGCAGAGTATGAGTGATGAGacaa                                                                                   <  1:553790/300‑2 (MQ=255)                                                                                                                                                                                                                           cTCGAGCAGGCAGTCGCGCGGGAAGTGATGGNAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATNTGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCAc                                                                   >  2:496641/1‑300 (MQ=255)                                                                                                                                                                                                                                 caggcagTCGCGCGGGAAGTGATGTAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGa                                                             <  2:259896/300‑1 (MQ=255)                                                                                                                                                                                                                                 caggcagTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGa                                                             <  2:532377/300‑1 (MQ=255)                                                                                                                                                                                                                                                   gTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGNCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGaa                                           >  1:491043/1‑300 (MQ=255)                                                                                                                                                                                                                                                    tGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAAc                                          >  1:648937/1‑300 (MQ=255)                                                                                                                                                                                                                                                    tGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGCTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATNAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGNACGAAc                                          >  2:573414/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      ttAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCTGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATc                        <  1:636567/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         ttACGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCa     >  1:554110/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            cGTGACTTCTCAGTCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGTCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGcc  <  2:380522/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                               CGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCCCCTGCATTATTGTTGCCATCCGTCGCGATGATTCGATCCTCCTCGCCCAGCATACCCGCCATCGTAACGGTGTCCATACAGTACTTGCCGGATTCGTCGAAGTGGGCGAAACCCTCGAGCAGGCAGTCGCGCGGGAAGTGATGGAAGAGAGCGGAATTAAAGTTAAAAACTTGCGTTACGTGACTTCTCAGCCGTGGCCGTTTCCTCAGTCTTTAATGACCGCGTTTATGGCGGAATATGACAGCGGCGACATCGTGATCGACCCGAAAGAATTGCTCGAGGCGAACTGGTATCGCTATGACGATTTGCCGTTACTCCCGCCGCCCGGCACCGTAGCGCGCCGTCTGATAGAAGATACGGTGGCGATGTGTCGGGCAGAGTATGAGTGATGATACACTGACCGCCTGACGCACTAAGGAACAGCCAAAATGACCGAACTTAAAAACGATCGTTATCTGCGGGCGCTGCTGCGCCAGCC  >  NC_000913/4197183‑4197765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑