Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,160,102	C→T	S230N (AGT→AAT)	sthA ←	soluble pyridine nucleotide transhydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,160,102	0	C	T	100.0%	302.7 / NA	87	S230N (AGT→AAT)	sthA	soluble pyridine nucleotide transhydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/35);  total (52/35)

ATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGC  >  NC_000913/4159808‑4160393                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                    aTGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTc                                                                                                                                                                                                                                                                                                <  1:230992/300‑1 (MQ=255)      ccccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTNGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGNCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCAAGaa                                                                                                                                                                                                                                                                                           >  2:541305/1‑300 (MQ=255)      ccccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGaa                                                                                                                                                                                                                                                                                           >  1:65949/1‑300 (MQ=255)       cccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCATGTCGCCCACCGCGGAAACGTGTGGCTGGGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAg                                                                                                                                                                                                                                                                                          <  1:259263/300‑1 (MQ=255)       cccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCNGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAg                                                                                                                                                                                                                                                                                          <  1:468473/300‑1 (MQ=255)       cccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTTTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAg                                                                                                                                                                                                                                                                                          <  1:559574/300‑1 (MQ=255)       cccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTTACGAATCACTACGCCATTGTTCCAGAAg                                                                                                                                                                                                                                                                                          <  2:359245/300‑1 (MQ=255)       cccTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAg                                                                                                                                                                                                                                                                                          <  1:565612/300‑1 (MQ=255)            gTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATTACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATa                                                                                                                                                                                                                                                                                     >  1:39280/1‑300 (MQ=255)              ctgagatgtgtataagagacagcGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAgg                                                                                                                                                                                                                                                                                   <  1:680625/278‑1 (MQ=255)              cggagatgtgtataagagacagcGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAgg                                                                                                                                                                                                                                                                                   <  1:680619/278‑1 (MQ=255)              cATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAgg                                                                                                                                                                                                                                                                                   <  1:112697/300‑1 (MQ=255)                tAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGGAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGgag                                                                                                                                                                                                                                                                                 <  1:382631/300‑1 (MQ=255)                          aCGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCNGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTg                                                                                                                                                                                                                                                                       >  1:463540/1‑300 (MQ=255)                                    cGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAggctgtctcttatacacatctcgc                                                                                                                                                                                                                                                             >  2:664430/1‑278 (MQ=255)                                    cGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTg                                                                                                                                                                                                                                                             >  1:572266/1‑300 (MQ=255)                                    cGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAggctgtctcttatacacatctagc                                                                                                                                                                                                                                                             >  2:664436/1‑278 (MQ=255)                                          actaaTCACGTCGCCCACCGCGTAAACGTGTNGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGACTCGGTATTANCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGCAGTGATAGGAGAGAGAATCTGACATCTCTTGATCgag                                                                                                                                                                                                                                                       >  2:497976/4‑300 (MQ=255)                                                             gcgTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGGGAGAGAATCTGACATCTATTGCTCGAGAACTGCCAGCAGGCGATcg                                                                                                                                                                                                                                    >  2:120656/1‑300 (MQ=255)                                                             gcgTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATcg                                                                                                                                                                                                                                    >  1:522823/1‑300 (MQ=255)                                                             gcgTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCAAGCAGGCGATcg                                                                                                                                                                                                                                    >  1:309686/1‑300 (MQ=255)                                                                     gtgtGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCAAAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGtt                                                                                                                                                                                                                            >  1:311523/1‑300 (MQ=255)                                                                          gCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCa                                                                                                                                                                                                                       >  1:608368/1‑300 (MQ=255)                                                                              tgaggTCTGATACATGCTGTTTACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGGCATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATc                                                                                                                                                                                                                   <  2:234715/297‑1 (MQ=255)                                                                                  gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                                                                                                                                               >  1:594651/1‑300 (MQ=255)                                                                                  gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                                                                                                                                               >  1:695647/1‑300 (MQ=255)                                                                                  gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATccccc                                                                                                                                                                                                               >  1:153037/1‑297 (MQ=255)                                                                                  gTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACt                                                                                                                                                                                                               >  1:616972/1‑300 (MQ=255)                                                                                    cTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGCGAATCTGACATCTCTTGATCGAGAAGTGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACttt                                                                                                                                                                                                             >  2:199319/1‑300 (MQ=255)                                                                                      gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa                                                                                                                                                                                                           >  1:678804/1‑300 (MQ=255)                                                                                      gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGCGATGGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGTAGGCGATCGCGGGTGTTGATCAGGTCCACTTTTa                                                                                                                                                                                                           >  2:77279/1‑300 (MQ=255)                                                                                      gATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTNTTACCCGACTTCAGATGCATGATCACACCATCGTCAAAGCCTTCGATCTTCTCGTACTCTTNGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGACCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTa                                                                                                                                                                                                           >  2:554847/1‑300 (MQ=255)                                                                                                    aCCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCgg                                                                                                                                                                                             >  2:384026/1‑300 (MQ=255)                                                                                                    aCCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTAAGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCgg                                                                                                                                                                                             >  1:149980/1‑300 (MQ=255)                                                                                                    aCCTTCAGCTGTCCACGGCTGTCAGTTTCTAGCCCAATGTTCTGTAGCGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTAACCGAATTCAGATGCATGATCAAACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACTGCgg                                                                                                                                                                                             >  2:63603/1‑300 (MQ=255)                                                                                                       ttCAGCTGTCCGCGGCGGTCAGTTTCGAGCCCAATTTTCTGTAACGCCAGCGAANCGGTATTACCGGTGCGACCGTTGGCATAGAGCATGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCTAGAAATNCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGTGGAAg                                                                                                                                                                                          <  2:644477/300‑1 (MQ=255)                                                                                                              gTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATTACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCAATTTTACATCCATACCGCGGAAGATCGaag                                                                                                                                                                                   >  2:44256/1‑298 (MQ=255)                                                                                                                       tGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAATTGATAGGATAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATGcac                                                                                                                                                                          <  1:202342/300‑1 (MQ=255)                                                                                                                       tGTCAGTGTCTAGCCCAATGCTCCGTAACACCAGCGAATCGGTGTTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCGGTTTTTTACCCGACTTCATATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTTTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGTGATCGCGGGTGTTGATCAGATCCATTTTTACATCCATACCGCGGAAGATCGACGCATATTcac                                                                                                                                                                          <  2:280031/300‑1 (MQ=255)                                                                                                                          cAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTNTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCNATACCGCGGAAGATCGACGCATATTCACAGc                                                                                                                                                                       <  2:497050/300‑1 (MQ=255)                                                                                                                            gTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTAACGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCAATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCg                                                                                                                                                                     >  2:671589/1‑300 (MQ=255)                                                                                                                               tCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATc                                                                                                                                                                  <  1:169009/300‑1 (MQ=255)                                                                                                                                 tatcccAATGTTCTTTAACGCCAGCGAATCGGTATTACCGGGGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCAc                                                                                                                                                                <  2:532023/297‑1 (MQ=255)                                                                                                                                    cccAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTcc                                                                                                                                                             >  1:377500/1‑300 (MQ=255)                                                                                                                                         tGTTCTGTAACGCCAGCGAGTCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTTTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAAATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                        <  2:52897/300‑1 (MQ=255)                                                                                                                                         tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGAGTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTTTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCTAGAAATGCCAGCATGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                        <  2:587532/300‑1 (MQ=255)                                                                                                                                         tGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                        <  2:381022/300‑1 (MQ=255)                                                                                                                                         tGTTCTGTAACGCCAGCGAAGCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAc                                                                                                                                                        <  1:126995/300‑1 (MQ=255)                                                                                                                                          gTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAcc                                                                                                                                                       >  1:57843/1‑300 (MQ=255)                                                                                                                                          gTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCAcc                                                                                                                                                       >  2:660179/1‑300 (MQ=255)                                                                                                                                                 aacaccAGCGAATCGTTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCATATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGTAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                                                                                                                                                <  2:272816/296‑1 (MQ=255)                                                                                                                                                 aaCGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATa                                                                                                                                                <  1:219318/300‑1 (MQ=255)                                                                                                                                                      cAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCTGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTNGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTNACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTAc                                                                                                                                           <  2:552069/300‑1 (MQ=255)                                                                                                                                                        gCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTNTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACAt                                                                                                                                         <  1:470560/300‑1 (MQ=255)                                                                                                                                                           aaTCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGgc                                                                                                                                      <  1:720/300‑1 (MQ=255)                                                                                                                                                                gTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCAAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCTATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGt                                                                                                                                 >  2:180623/1‑300 (MQ=255)                                                                                                                                                                       cGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgtggt                                                                                                                          <  1:335905/300‑1 (MQ=255)                                                                                                                                                                       cGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTCTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTCGTGACGAATCACTACGCCATTGTTCCATAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGCGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCgtggt                                                                                                                          <  2:366215/300‑1 (MQ=255)                                                                                                                                                                         gTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTATTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGc                                                                                                                        >  2:670583/1‑300 (MQ=255)                                                                                                                                                                                cGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTga                                                                                                                 >  1:684114/1‑300 (MQ=255)                                                                                                                                                                                  ttGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTgaga                                                                                                               >  1:306/1‑300 (MQ=255)                                                                                                                                                                                    ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt                                                                                                             >  1:572668/1‑300 (MQ=255)                                                                                                                                                                                    ggCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt                                                                                                             >  2:407014/1‑300 (MQ=255)                                                                                                                                                                                    ggCATAGAGCAGGCAGTCAGCTTGCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAt                                                                                                             <  2:266109/300‑1 (MQ=255)                                                                                                                                                                                     gCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAAtt                                                                                                            >  1:40757/1‑300 (MQ=255)                                                                                                                                                                                          gagCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTc                                                                                                       >  2:337566/1‑300 (MQ=255)                                                                                                                                                                                                  agTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTNACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGt                                                                                               >  1:508419/1‑300 (MQ=255)                                                                                                                                                                                                       gCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTTTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGATAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATg                                                                                          <  2:1533/300‑1 (MQ=255)                                                                                                                                                                                                                                        aTCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTAAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgatga                                                         >  2:367203/1‑300 (MQ=255)                                                                                                                                                                                                                                         tCACACCATCGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGgatgac                                                        >  1:73492/1‑299 (MQ=255)                                                                                                                                                                                                                                                aTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACg                                                 <  2:306586/300‑1 (MQ=255)                                                                                                                                                                                                                                                aTCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAANGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACACCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACg                                                 <  2:602964/300‑1 (MQ=255)                                                                                                                                                                                                                                                  cGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACgag                                               >  1:445326/1‑300 (MQ=255)                                                                                                                                                                                                                                                  cGTCACAGCCTTCGATCTTCTAGTACTCTTCGTTGTGACGAATCACTACGAAATTGTTCCAGACGTGATAGGAGGGAGAATCTGACCTCTATTGATCGAGAAATGCAAGCAGGCGATCGCGGGTATTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATCTTCACAGCCGATCACTCACGCACAATCGATAAGTACATGGTGCGGTTAGTGATGCCTGCTGAGAATCGAGCCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATTTGTTGGCTGatatcgatgcg                                               >  2:141975/1‑293 (MQ=255)                                                                                                                                                                                                                                                   gTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACgaga                                              >  1:240557/1‑300 (MQ=255)                                                                                                                                                                                                                                                     cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGc                                            <  1:414535/300‑1 (MQ=255)                                                                                                                                                                                                                                                     cacaGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGc                                            <  2:622850/300‑1 (MQ=255)                                                                                                                                                                                                                                                          ccTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAg                                       >  1:428294/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cttctCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCCGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAAc                               >  2:68132/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ttctCGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACa                              >  2:396054/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cGTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACCTCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAAtt                          >  1:523433/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTACTCTTCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGNGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAAttt                         >  1:486669/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               tCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTNGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCg                  >  1:507811/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               tCGTTGTGACGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTTGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCg                  >  1:687583/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       aCGAATCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAgtgt          <  1:135346/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            tCACTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCaa     >  2:412555/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cTACGCCATTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCAAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGc  >  2:179386/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                    ATGCGCCCCTGGTCATAGGCCGCCGACGCCAGGCTCGGATAACCAATCACGTCGCCCACCGCGTAAACGTGTGGCTGTGCGGTCTGATACATGCTGTTGACCTTCAGCTGTCCGCGGCTGTCAGTTTCTAGCCCAATGTTCTGTAACGCCAGCGAATCGGTATTACCGGTGCGACCGTTGGCATAGAGCAGGCAGTCAGCTTTCAGTTTTTTACCCGACTTCAGATGCATGATCACACCATCGTCACAGCCTTCGATCTTCTCGTACTCTTCGTTGTGACGAATCACTACGCCACTGTTCCAGAAGTGATAGGAGAGAGAATCTGACATCTCTTGATCGAGAAATGCCAGCAGGCGATCGCGGGTGTTGATCAGATCCACTTTTACATCCATACCGCGGAAGATCGACGCATATTCACAGCCGATCACTCCAGCACCATAGATAAGTACATGGCGCGGTTCGTGGTGCATGCTGAGAATTGAGTCGCTGTCGTAAATGCGTGGATGGGTGAAATCAACATCTGTTGGATGATATGGACGAGAGCCGCAGGCAATAACAAATTTTTCAGCGGTTAGTGTTTCAACGC  >  NC_000913/4159808‑4160393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑