Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,130,929	C→T	T365T (ACC→ACT)	metL →	fused aspartate kinase/homoserine dehydrogenase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,130,929	0	C	T	100.0%	191.3 / NA	57	T365T (ACC→ACT)	metL	fused aspartate kinase/homoserine dehydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/31);  total (26/31)

CTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACCTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTAT  >  NC_000913/4130636‑4131221                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                     cttcACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGaa                                                                                                                                                                                                                                                                                                >  1:198781/1‑300 (MQ=255)  ttcACGCCCGTACTTTACAGCCGTTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTAATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCATAAg                                                                                                                                                                                                                                                                                               <  2:332647/300‑1 (MQ=255)  ttcACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAg                                                                                                                                                                                                                                                                                               <  1:271351/300‑1 (MQ=255)  ttcACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAg                                                                                                                                                                                                                                                                                               <  2:588618/300‑1 (MQ=255)  ttaaCGCCCGTACTGTACAGCCGGTTTATGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGAGCAAGGTTCCACGCGCATGGAACGCGTGCTGGACTCCGGTACTGGGGCGCGTATTGTCACCAGCCACGATGATGTCGGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAg                                                                                                                                                                                                                                                                                               <  1:694958/297‑1 (MQ=255)      cGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGc                                                                                                                                                                                                                                                                                           >  1:288098/1‑300 (MQ=255)      cGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTAAACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCAACAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATCTCAAACTGGCGCATAAAGAGATCGAACAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGCACATAACGATCGACAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGc                                                                                                                                                                                                                                                                                           >  2:228939/1‑300 (MQ=255)          cGTACTTTACAGCCGGTTTCGGGCGGCGAAATCGACCTGCGACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGAc                                                                                                                                                                                                                                                                                       <  1:214738/300‑1 (MQ=255)            ttcTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTACGCTTTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATNTCAAACTGGCGCATAAAGAGATCGACCAAATCCTTAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGNAATTTTCCTACACTTCATAAGTTGcctaccc                                                                                                                                                                                                                                                                                     <  2:490429/298‑6 (MQ=255)            tACTTTACAGCCGGTTGCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATNTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGNAATTTTGCTACACTTCAGAAGTGGCCGACAg                                                                                                                                                                                                                                                                                     <  2:484786/300‑1 (MQ=255)                                      aaaTCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCNGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGANCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCgg                                                                                                                                                                                                                                                           <  2:549643/300‑1 (MQ=255)                                              cTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCt                                                                                                                                                                                                                                                   <  1:371031/300‑1 (MQ=255)                                                           gTAGCTACACGCCGGATCAAGGTTCCACGCGNATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCANGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTgcgca                                                                                                                                                                                                                                      >  2:500779/1‑299 (MQ=255)                                                           gTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGcc                                                                                                                                                                                                                                      >  1:315335/1‑300 (MQ=255)                                                                             aaGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGgcgc                                                                                                                                                                                                                    <  1:669073/300‑1 (MQ=255)                                                                                gTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCtgg                                                                                                                                                                                                                 >  1:415052/1‑300 (MQ=255)                                                                                           cggagatgtgtataagagacaggTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCNTAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTc                                                                                                                                                                                                      <  1:479348/279‑1 (MQ=255)                                                                                            ttGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCAACAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGAATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCg                                                                                                                                                                                                     >  2:613989/1‑300 (MQ=255)                                                                                                aCGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGc                                                                                                                                                                                                 <  1:228363/300‑1 (MQ=255)                                                                                                     tgctttccTCCGGTACTGGTGCGCGTATTGTCACCAGCCACTATGATTTCTGTTTGATTGAGTTTCATGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATTGCCAGTTGCTGCAATTTTGCTATACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCg                                                                                                                                                                                            <  2:326096/294‑1 (MQ=255)                                                                                                     tGCTGTCCTCCGGTACTGGTGTGCGTATTGTCACCAGCCACGATAATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTTAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGTGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCg                                                                                                                                                                                            <  2:265841/300‑1 (MQ=255)                                                                                                     tGCTGGCCTCCTGTACTGGTGCTCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGNGATCGACCAAATCCTTAAACGCGCGCATGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCg                                                                                                                                                                                            <  2:597020/300‑1 (MQ=255)                                                                                                     tGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCg                                                                                                                                                                                            <  1:220891/300‑1 (MQ=255)                                                                                                               cGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTaa                                                                                                                                                                                  >  1:651330/1‑300 (MQ=255)                                                                                                                 ttACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCATGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCACTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAAcc                                                                                                                                                                                <  2:383595/299‑1 (MQ=255)                                                                                                                 gTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTNAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAAcc                                                                                                                                                                                <  1:471508/300‑1 (MQ=255)                                                                                                                 gTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTNCGCCCGCTGGCGGTTGGCGTACATAANGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTcctgtctcttatacacatctcgc                                                                                                                                                                                >  2:469288/1‑278 (MQ=255)                                                                                                                 gTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAAcc                                                                                                                                                                                >  1:553989/1‑300 (MQ=255)                                                                                                                 gTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCAGAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAAcc                                                                                                                                                                                <  1:446654/300‑1 (MQ=255)                                                                                                                 gTACTGGTGCGCGTATTGTCACCAGCCACGATGAGGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCACCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAAcc                                                                                                                                                                                <  2:207341/300‑1 (MQ=255)                                                                                                                          cgcgTATTGTCACCAGCCACGATGATGTCTGTTTGATTGCGTTTCCGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGTGTACATAACGATCGCAAGTTGCTGCAATTTTGCTACACTTAAGAAGTGGCCGACCGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCATGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTgtact                                                                                                                                                                       >  2:389826/1‑296 (MQ=255)                                                                                                                          cgcgTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGGGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGAAGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCAGTAACCCGCTGCAtt                                                                                                                                                                       >  2:336859/1‑300 (MQ=255)                                                                                                                                  gTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGc                                                                                                                                                               >  1:290165/1‑300 (MQ=255)                                                                                                                                              gatgatGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCaa                                                                                                                                                   >  1:209816/1‑300 (MQ=255)                                                                                                                                                  atgTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGa                                                                                                                                               >  1:329373/1‑300 (MQ=255)                                                                                                                                                        gTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTAGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTAAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCAGCTGCATTACCACCGCTTCTGGCAGCAACTAAAAGGcc                                                                                                                                         >  2:102505/1‑300 (MQ=255)                                                                                                                                                                 aGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGCACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCg                                                                                                                                >  1:534472/1‑300 (MQ=255)                                                                                                                                                                                                  tGGCGCATAAAGAGAACGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGTCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGcc                                                                                               <  1:187292/300‑1 (MQ=255)                                                                                                                                                                                                                     ccAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCAc                                                                            <  1:563415/300‑1 (MQ=255)                                                                                                                                                                                                                     ccAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCNCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGNTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCAc                                                                            <  2:542219/300‑1 (MQ=255)                                                                                                                                                                                                                         aTCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGc                                                                        >  1:52645/1‑300 (MQ=255)                                                                                                                                                                                                                          tCCTGANACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCNGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCACGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGcc                                                                       <  2:576479/300‑1 (MQ=255)                                                                                                                                                                                                                          tCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGTTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGcc                                                                       <  2:189989/300‑1 (MQ=255)                                                                                                                                                                                                                                      cgcAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGcc                                                           >  1:335525/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGAt                                                       >  1:196419/1‑300 (MQ=255)                                                                                                                                                                                                                                               gCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAgg                                                  >  1:50188/1‑300 (MQ=255)                                                                                                                                                                                                                                                  cGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACTAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGTTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGTTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGc                                               <  2:143698/300‑1 (MQ=255)                                                                                                                                                                                                                                                          gTTGGCGTACATAACGATCGCCATTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAg                                       <  2:582519/300‑1 (MQ=255)                                                                                                                                                                                                                                                             ggCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTcc                                    >  1:372936/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tACATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCt                                >  1:246984/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCtt                               >  2:586018/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   cATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCNCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTAACTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTNCTGCGCACCGGCCCGACCGAAAGCATGATTCAGGGGCTGCATCAGTCCGTCTTc                              >  2:643895/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   cATAACGATCGCCAGTTGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTc                              >  1:382566/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  ttGCTGCAATTTTGCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAATCCGTCTTCCGCGCAGAAAAACGc               <  1:369811/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gCTACACTTCAGAAGTTGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATNACCTGGCGAACTGCGCCTGCGTCATGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGGCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGGCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTAt  <  2:514182/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGAGTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGatat  <  1:629154/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gCTACACTTCAGAAGTGGCCGACAGTGCGCTGAAAACCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGGCGATGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCCGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTAt  <  2:52457/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                     CTTCACGCCCGTACTTTACAGCCGGTTTCTGGCAGCGAAATCGACCTGCAACTGCGCTGTAGCTACACGCCGGATCAAGGTTCCACGCGCATTGAACGCGTGCTGGCCTCCGGTACTGGTGCGCGTATTGTCACCAGCCACGATGATGTCTGTTTGATTGAGTTTCAGGTGCCCGCCAGTCAGGATTTCAAACTGGCGCATAAAGAGATCGACCAAATCCTGAAACGCGCGCAGGTACGCCCGCTGGCGGTTGGCGTACATAACGATCGCCAGTTGCTGCAATTTTGCTACACCTCAGAAGTGGCCGACAGTGCGCTGAAAATCCTCGACGAAGCGGGATTACCTGGCGAACTGCGCCTGCGTCAGGGGCTGGCGCTGGTGGCGATGGTCGGTGCAGGCGTCACCCGTAACCCGCTGCATTGCCACCGCTTCTGGCAGCAACTGAAAGGCCAGCCGGTCGAATTTACCTGGCAGTCCGATGACGGCATCAGCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTAT  >  NC_000913/4130636‑4131221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑