Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,042,171	G→A	E35K (GAG→AAG)	yihD →	DUF1040 domain‑containing protein YihD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,042,171	0	G	A	100.0%	317.4 / NA	92	E35K (GAG→AAG)	yihD	DUF1040 domain‑containing protein YihD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/44);  total (48/44)

CGTCAGCGACATGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATAC  >  NC_000913/4041876‑4042458                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                cgTCAGCGACATGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGACTATTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTAAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTc                                                                                                                                                                                                                                                                                             >  1:226547/1‑300 (MQ=255)     aGCGACATGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGt                                                                                                                                                                                                                                                                                         >  1:166027/1‑300 (MQ=255)     aGCGACATGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTANGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGt                                                                                                                                                                                                                                                                                         >  1:468145/1‑300 (MQ=255)      gCGACATGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGtt                                                                                                                                                                                                                                                                                        >  1:449123/1‑300 (MQ=255)           aTGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGAc                                                                                                                                                                                                                                                                                   >  2:446772/1‑300 (MQ=255)           aTGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGAc                                                                                                                                                                                                                                                                                   <  1:408516/300‑1 (MQ=255)                 ccATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTNTTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTNAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGaa                                                                                                                                                                                                                                                                             >  2:546144/1‑300 (MQ=255)                      aTGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTCTCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCAATATGAAATGTAAACGTATGAATGAAGTTATTGAACTCCTACAGCCAGCCTGGCAAAAAGCGCCAGACCTTACCCTGCTGCAATTTTTGCAGAGACTGGCGAAAAAGTAGGGTTTTGACGGCGAATTGGc                                                                                                                                                                                                                                                                        >  2:364888/1‑300 (MQ=255)                            tGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGAttt                                                                                                                                                                                                                                                                  <  1:406892/300‑1 (MQ=255)                                gTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTACAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACg                                                                                                                                                                                                                                                              >  1:186527/1‑300 (MQ=255)                                             aaTCCTTTATCTACGCCACCCACTCGTCTGGCTTTACCACCTACCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTTTTGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTTGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGa                                                                                                                                                                                                                                                 <  2:140457/300‑1 (MQ=255)                                              aTCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTCTTGTGGGATTGACCCTGCTCACGTGTCTNTCTCAAGAGTAAGGAGCATCAATCTGACATGTAAACGTCTGAATGAAGTTATTGCACTCCTNCAGCCAGCCTGGCAAAAAGAGCAAGACCTTCACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGac                                                                                                                                                                                                                                                >  2:541033/1‑299 (MQ=255)                                              aTCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGCCCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGAt                                                                                                                                                                                                                                                >  2:90257/1‑300 (MQ=255)                                              aTCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGAt                                                                                                                                                                                                                                                >  1:389397/1‑300 (MQ=255)                                                ccTTTATCTCCGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAAACTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACCGGCGGATTTGAAGGATGACATTCTAATCt                                                                                                                                                                                                                                              >  2:231083/1‑300 (MQ=255)                                                ccTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCt                                                                                                                                                                                                                                              >  1:343182/1‑300 (MQ=255)                                                ccTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAAACTGCTGCAATTTTTGCAGCAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCt                                                                                                                                                                                                                                              >  2:344450/1‑300 (MQ=255)                                                    tATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTNACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCa                                                                                                                                                                                                                                          <  1:467757/300‑1 (MQ=255)                                                    tATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCa                                                                                                                                                                                                                                          <  1:111756/300‑1 (MQ=255)                                                     aTCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCCGCAATTTTTGCAGAAACTGGCGAAAAANTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCAc                                                                                                                                                                                                                                         >  2:512639/1‑300 (MQ=255)                                                             cgccTATTCGTCTGGCTCTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTTTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATTAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAAt                                                                                                                                                                                                                                 <  2:339172/300‑1 (MQ=255)                                                                     cGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGAtt                                                                                                                                                                                                                         >  1:349063/1‑300 (MQ=255)                                                                               tACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGa                                                                                                                                                                                                               <  1:246982/300‑1 (MQ=255)                                                                                      tGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAACCTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTg                                                                                                                                                                                                        >  1:24712/1‑300 (MQ=255)                                                                                      tGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCCCCTGAAAATGCGTGATTCCGCGAAAGATGCGGTg                                                                                                                                                                                                        >  2:615704/1‑300 (MQ=255)                                                                                      tGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTg                                                                                                                                                                                                        >  1:24817/1‑300 (MQ=255)                                                                                       gCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGTCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGa                                                                                                                                                                                                       <  1:253528/300‑1 (MQ=255)                                                                                       gCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGa                                                                                                                                                                                                       <  1:401456/300‑1 (MQ=255)                                                                                           gCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATccc                                                                                                                                                                                                   >  1:416625/1‑300 (MQ=255)                                                                                              caACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGNTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGNGTGATTCCGCGAAAGATGCGGTGATCCCggg                                                                                                                                                                                                <  2:481716/300‑1 (MQ=255)                                                                                              caACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCggg                                                                                                                                                                                                <  1:540910/300‑1 (MQ=255)                                                                                              caACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCAAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTAAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCggg                                                                                                                                                                                                >  2:121129/1‑300 (MQ=255)                                                                                               aaCGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGt                                                                                                                                                                                               <  1:690509/300‑1 (MQ=255)                                                                                                aCGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGtt                                                                                                                                                                                              <  1:73858/300‑1 (MQ=255)                                                                                                aCGCCTCTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGCCAAAAAGAGCCAGACCTTAACCTGCTNCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGNGATTCCGCGAAAGATGCGGTGATCCCGGGtt                                                                                                                                                                                              <  2:477873/300‑1 (MQ=255)                                                                                                    cTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGNCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCa                                                                                                                                                                                          >  1:508116/1‑300 (MQ=255)                                                                                                                                   cGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGgcg                                                                                                                                                           >  1:575069/1‑300 (MQ=255)                                                                                                                                        ctTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGtt                                                                                                                                                      >  1:392583/1‑300 (MQ=255)                                                                                                                                            tATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTAcgc                                                                                                                                                  <  2:208370/300‑1 (MQ=255)                                                                                                                                              ttGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACgcgc                                                                                                                                                <  1:302640/300‑1 (MQ=255)                                                                                                                                                gtgGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGTAGAAGACTTCAAAACGGCGCTGTTACGCGCTc                                                                                                                                              >  1:98957/1‑300 (MQ=255)                                                                                                                                                    gATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCgcgg                                                                                                                                          >  1:114950/1‑300 (MQ=255)                                                                                                                                                    gATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTGGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTGGACGGATGACAGTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCATAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCgcgg                                                                                                                                          <  1:179799/300‑1 (MQ=255)                                                                                                                                                            cTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGNATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGNAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTa                                                                                                                                  >  2:637581/1‑300 (MQ=255)                                                                                                                                                            cTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATNTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTa                                                                                                                                  >  1:509914/1‑300 (MQ=255)                                                                                                                                                            cTGCTAACGTGTCTGTCTAAAGAGTAAGGAGCATCACTATGAAATGTAGACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAAACTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATCCCGCGAAAGATGCGGTGCTCCAGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTATTACGCGCTCGCGGCGTAATTa                                                                                                                                  >  2:124965/1‑300 (MQ=255)                                                                                                                                                                 aaCGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAagag                                                                                                                             <  2:298937/300‑1 (MQ=255)                                                                                                                                                                 aaCGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAagag                                                                                                                             <  1:226491/300‑1 (MQ=255)                                                                                                                                                                 aaCGTGTCTGTCTCAAGAGTAAGGAGCATCAATATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCAGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAagag                                                                                                                             >  1:199686/1‑300 (MQ=255)                                                                                                                                                                       tctgtctCAAGAGTAAGGAGCATCACTATGAAATATAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAg                                                                                                                       <  1:533238/300‑1 (MQ=255)                                                                                                                                                                            ctcAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGCTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGt                                                                                                                  >  2:348037/1‑300 (MQ=255)                                                                                                                                                                              cAAGAGTAAGGAGCATCACTATGAAATGTAANCGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAANTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTaa                                                                                                                >  2:495218/1‑300 (MQ=255)                                                                                                                                                                               aaGAGTNAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTNCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAg                                                                                                               <  2:564663/300‑1 (MQ=255)                                                                                                                                                                                agagTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAgc                                                                                                              <  2:239645/300‑1 (MQ=255)                                                                                                                                                                                agagTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAgc                                                                                                              <  1:235475/300‑1 (MQ=255)                                                                                                                                                                                agagTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACCTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAgc                                                                                                              <  2:432881/300‑1 (MQ=255)                                                                                                                                                                                 gagTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCNAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTNTAAgcg                                                                                                             >  2:562069/1‑300 (MQ=255)                                                                                                                                                                                 gagTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAgcg                                                                                                             >  1:159273/1‑300 (MQ=255)                                                                                                                                                                                 gagTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCAGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAgcg                                                                                                             >  1:36981/1‑300 (MQ=255)                                                                                                                                                                                        gAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGccac                                                                                                      >  1:445617/1‑300 (MQ=255)                                                                                                                                                                                               aCTATGAAATGTAAACTTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATACGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAatc                                                                                               <  2:292935/300‑1 (MQ=255)                                                                                                                                                                                                cTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAatca                                                                                              >  1:352257/1‑300 (MQ=255)                                                                                                                                                                                                                tCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACTGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATTAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATcc                                                                              <  2:668610/300‑1 (MQ=255)                                                                                                                                                                                                                tCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATcc                                                                              <  2:314819/300‑1 (MQ=255)                                                                                                                                                                                                                tCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATcc                                                                              <  1:164240/300‑1 (MQ=255)                                                                                                                                                                                                                   gaatgaaGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTc                                                                           >  2:295235/1‑300 (MQ=255)                                                                                                                                                                                                                       gaaGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGNTTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCa                                                                       <  1:473443/300‑1 (MQ=255)                                                                                                                                                                                                                                gaattcctccAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTGTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATg                                                              <  2:64979/296‑1 (MQ=255)                                                                                                                                                                                                                                     cctccAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGACATGATATCCTTCGTCATTCGTAATGTTTTc                                                         >  2:663811/1‑300 (MQ=255)                                                                                                                                                                                                                                     cctccAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTc                                                         >  1:26018/1‑300 (MQ=255)                                                                                                                                                                                                                                                         aaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAAc                                     <  2:114889/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gagCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCCTGGAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAg                                   <  2:291203/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  aCCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCNAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGANATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt                            >  2:631094/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt                            >  1:100993/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt                            >  2:281351/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTAAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCACtttt                            >  2:57021/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCAGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCtttt                            >  1:196899/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ccTTAACCTGCTGCAATTTTTGCAGAAACTGTCGAAAAAGTCAGTTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCNGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa                           <  2:158820/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   ccTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa                           >  2:208621/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ccTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa                           >  1:296353/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ccTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAANTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTa                           >  2:605705/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ctgcAATTTTTGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGac                 <  1:243520/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              tgcAATTTTTGCAGAAACTTGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCTGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCACCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGaca                <  2:353181/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ttttGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGAGTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATGCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAAATTATGAAGAAGACTTCAAAACGGCGCTTTTACGCGCTCGCGGCGTAATTAAAGAGTAGAAGCTTGGAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAgacacgacac          <  2:330091/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tttGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGGTATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc         <  1:456424/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tttGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc         <  1:74987/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tttGCAAAAACTGTCGAAAAAGTCAGGTTTTGACGGCGAACTTGCTGGTTTGACGGAGGACATTCTGATCTATCGCCTGAAAATGTGTGATTCCGCTAAAGATGCGTTGATCCCGGGTTTGCAGAAAGATTANGAAGAAGACTTCAAAACGGCGCTGTTACACGCTCGCGGCGTAATTAAAGAGTAGAAGCCTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACAcc         <  2:152485/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tGCAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCg       <  1:253502/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tGCAGAAACGGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCg       <  1:330958/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cAGAAACTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCTGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGa     <  2:200126/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aGAAACTGGCGAAAAAGTCAGGTTGTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGGGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGAt    <  1:232727/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aaaCTGGCGAAAAAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATAc  <  1:645784/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                CGTCAGCGACATGTTGCCATAATGGTTTGCCGTTTAATTCAAGCAATCCTTTATCTACGCCGCCCATTCGTCTGGCTTTACCGCCTGCCAGCACAACGCCTGTTATCGTCGTCATCAGATTCACCGATATCGCCTCTTTTATTGTGGGATTGACCCTGCTAACGTGTCTGTCTCAAGAGTAAGGAGCATCACTATGAAATGTAAACGTCTGAATGAAGTTATTGAACTCCTCCAGCCAGCCTGGCAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGATCCCGGGTTTGCAGAAAGATTATGAAGAAGACTTCAAAACGGCGCTGTTACGCGCTCGCGGCGTAATTAAAGAGTAAAAGCTTGTAAGCGGCGCCACCAAAATCATCGTGAAATGATATCCTTCGTCATTCGTAATGTTTTCCGGATGATGGGATGAATAACAGCGCTTTTACTTTCCAGACACTACACCCGGATAC  >  NC_000913/4041876‑4042458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑