Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,031,554	G→A	V132M (GTG→ATG)	pepQ →	Xaa‑Pro dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,031,554	0	G	A	97.7%	296.7 / NA	86	V132M (GTG→ATG)	pepQ	Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/56);  minor base C (2/0);  total (30/56)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACAC  >  NC_000913/4031261‑4031849                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        aCTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGNGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCANTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCgg                                                                                                                                                                                                                                                                                                   <  2:646283/300‑1 (MQ=255)   tCCGGCNAGCTGTTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGNACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGaa                                                                                                                                                                                                                                                                                                 <  2:563174/300‑1 (MQ=255)   tCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGaa                                                                                                                                                                                                                                                                                                 <  1:598639/300‑1 (MQ=255)   tCCGGCGAGCTGGTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGTTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGaa                                                                                                                                                                                                                                                                                                 <  1:598488/300‑1 (MQ=255)         gAGCTGTTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTgcg                                                                                                                                                                                                                                                                                           >  1:652728/1‑300 (MQ=255)                tCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAAc                                                                                                                                                                                                                                                                                    <  1:177726/300‑1 (MQ=255)                    tGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTggg                                                                                                                                                                                                                                                                                <  2:440212/300‑1 (MQ=255)                            tcGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTTGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAgg                                                                                                                                                                                                                                                                        <  1:257186/300‑1 (MQ=255)                            tcGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAgg                                                                                                                                                                                                                                                                        <  2:60267/300‑1 (MQ=255)                            tcGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAgg                                                                                                                                                                                                                                                                        <  1:211965/300‑1 (MQ=255)                             cgacgaTCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGACAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGc                                                                                                                                                                                                                                                                       >  2:93528/1‑300 (MQ=255)                                cgaTCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAg                                                                                                                                                                                                                                                                    >  2:674248/1‑300 (MQ=255)                                      tcCCTCTCCGTTTAAAGTGAACCCGCAATTCNAAGCGTGGGTGTCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATNACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGCTCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGAAACAt                                                                                                                                                                                                                                                              >  2:481861/1‑300 (MQ=255)                                      tcCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTNTATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACAt                                                                                                                                                                                                                                                              >  2:512067/1‑300 (MQ=255)                                      tcCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGACTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACAt                                                                                                                                                                                                                                                              <  1:53859/300‑1 (MQ=255)                                              cGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGa                                                                                                                                                                                                                                                      >  1:230048/1‑300 (MQ=255)                                               gTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTNCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCNGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGaa                                                                                                                                                                                                                                                     >  2:646303/1‑300 (MQ=255)                                                    aaGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACTTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGTTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAgggg                                                                                                                                                                                                                                                <  1:266148/300‑1 (MQ=255)                                                         aaCCCGCAATTCAAAGCGTGGTTGCCGGTAACTCAGGTGCCAAACTGCGGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCAAAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGN‑AGATGTGGAAGTGATCGCGCTGCCGANAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATc                                                                                                                                                                                                                                           <  2:461089/300‑1 (MQ=255)                                                            ccGCAATTCAAATCGTGGGTGCCGGTAACTCAGGTGCCGAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAATTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGAc                                                                                                                                                                                                                                        <  1:531250/300‑1 (MQ=255)                                                            ccGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGAc                                                                                                                                                                                                                                        <  1:247051/300‑1 (MQ=255)                                                               cAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCTAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACATGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTAc                                                                                                                                                                                                                                     <  2:250698/300‑1 (MQ=255)                                                                 aTTCACAGCGTGGGTGCCGGTAACTAAGGTGCCAAACTGATGATTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCAGGTCGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGAAATGA‑AGATGTGGAAGTGATCGCGCTACCGCAAGCCGATGGCATTGGTCGCCTGCTGCCTGCTGCGCGCGGCAGTATCGGTTATATCGGTCCGCTGCCGGAACATACGCTGCCACTGGGTCTTGAGGCCAGCAACATCAACCAGAAAGGGGTTATCGACTACCt                                                                                                                                                                                                                                   >  1:86971/1‑300 (MQ=255)                                                                 aTTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCt                                                                                                                                                                                                                                   >  1:559188/1‑300 (MQ=255)                                                                      aaGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCTCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCAtt                                                                                                                                                                                                                              >  1:261437/1‑300 (MQ=255)                                                                      aaGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCAtt                                                                                                                                                                                                                              <  1:196039/300‑1 (MQ=255)                                                                       aGCGTGGGTGCCAGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGTAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTa                                                                                                                                                                                                                             <  2:142581/300‑1 (MQ=255)                                                                       aGCGTGGGTACCGGTAACTCAGGTGCCAAACTGCTGGTTGCGGGTGGATGGCGTGAACACGCCGAAACTGTGGTTCTATCTGCCGGTGGATTACTTGCACAACGTCGAACCGCTGCCGAT‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTa                                                                                                                                                                                                                             <  2:143334/300‑1 (MQ=255)                                                                        gCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTAc                                                                                                                                                                                                                            <  2:425211/300‑1 (MQ=255)                                                                        gCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTAc                                                                                                                                                                                                                            <  1:650953/300‑1 (MQ=255)                                                                                gCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGNC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTc                                                                                                                                                                                                                    <  1:511575/300‑1 (MQ=255)                                                                                gCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTc                                                                                                                                                                                                                    >  2:666626/1‑300 (MQ=255)                                                                                  cGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCt                                                                                                                                                                                                                  >  1:369225/1‑300 (MQ=255)                                                                                        cTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑GGCTTTCCAGACTGACAG‑GGTGGAGGGGCTCGCGCTGCCGAAAGCAGATGGCATTGGTAGTATGTTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGACAGCAACATCAACCCGAAAGGGGTTATCGACTAACTGTATTACTATCGCTCCTTCaaag                                                                                                                                                                                                            >  2:20946/1‑299 (MQ=255)                                                                                         tCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGNTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGNAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAAc                                                                                                                                                                                                           <  2:636441/300‑1 (MQ=255)                                                                                         tCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACTTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAAc                                                                                                                                                                                                           <  1:258716/300‑1 (MQ=255)                                                                                         tCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAAc                                                                                                                                                                                                           <  1:231463/300‑1 (MQ=255)                                                                                           aGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTg                                                                                                                                                                                                         <  1:216703/300‑1 (MQ=255)                                                                                            ggTGCCAAACTGCTGGTTGCTGGTGGATGGCNTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAANTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTCCAAAACTGa                                                                                                                                                                                                        >  2:492092/1‑300 (MQ=255)                                                                                             gTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAg                                                                                                                                                                                                       >  2:91661/1‑300 (MQ=255)                                                                                             gTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGNGATCGCGCTGCCGAAAGCCGATGGCANTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAg                                                                                                                                                                                                       >  2:470613/1‑300 (MQ=255)                                                                                                     ctgctgGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACt                                                                                                                                                                                               <  2:677612/300‑1 (MQ=255)                                                                                                     ctgctgGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGCACGAACt                                                                                                                                                                                               >  2:712/1‑300 (MQ=255)                                                                                                       gctggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTgg                                                                                                                                                                                             >  1:326937/1‑300 (MQ=255)                                                                                                         tggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTGTCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGcc                                                                                                                                                                                           <  2:373406/300‑1 (MQ=255)                                                                                                         tggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGcc                                                                                                                                                                                           <  1:447664/300‑1 (MQ=255)                                                                                                          ggttgctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCt                                                                                                                                                                                          <  1:37574/300‑1 (MQ=255)                                                                                                              gctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTAt                                                                                                                                                                                      <  1:585506/300‑1 (MQ=255)                                                                                                              gctggtGGATGGCGGGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCGGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTAt                                                                                                                                                                                      <  1:558565/300‑1 (MQ=255)                                                                                                               ctggtGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGCAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATg                                                                                                                                                                                     >  1:382539/1‑300 (MQ=255)                                                                                                                   tggATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTg                                                                                                                                                                                 <  1:411497/300‑1 (MQ=255)                                                                                                                        ggCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACACCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAgcg                                                                                                                                                                            >  2:421264/1‑300 (MQ=255)                                                                                                                                  aGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGNCGACGCTTCTTCAACATTGT‑AGCTCTA‑AATTGTTTTCGCCTCATCAAGCCGATGGCATTGGTAGCCTGCTGCATGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCAAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATNGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATgg                                                                                                                                                                  >  2:647935/1‑300 (MQ=255)                                                                                                                                          cTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAAc                                                                                                                                                          <  1:124604/300‑1 (MQ=255)                                                                                                                                            gtgGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGTTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAgctgtctcttatacacatctcgc                                                                                                                                                        <  1:269138/300‑22 (MQ=255)                                                                                                                                                    aTCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACcgcg                                                                                                                                                <  1:2816/300‑1 (MQ=255)                                                                                                                                                        gCCGGTTGATTACTGGCACAACTTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGc                                                                                                                                            <  1:376280/300‑1 (MQ=255)                                                                                                                                                            gTTGATTACTGACACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAACGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGCTGCCGGAACGTGCGCTGCCACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTCTCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTCTGCGTGAAGCGCCGAAAATGGCGGTCAACGGTCACCGCGCGGCagaa                                                                                                                                        >  1:146318/1‑300 (MQ=255)                                                                                                                                                             ttGATTACTTGCACAATGTCGAACCGCTTCCGAT‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCGGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAATGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCagaag                                                                                                                                       <  2:7691/300‑1 (MQ=255)                                                                                                                                                                    cTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGcgtt                                                                                                                                >  2:403788/1‑300 (MQ=255)                                                                                                                                                                     tGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGcgttc                                                                                                                               <  1:193235/300‑1 (MQ=255)                                                                                                                                                                            aCGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGACAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAAACCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTAAAAACTGAGTACGAACTGGCCTGTATGCGTGCAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTg                                                                                                                        >  1:570173/1‑300 (MQ=255)                                                                                                                                                                             cGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCAGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTCTGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCACGCGGCAGAAGACGCGTTCCGTTCTgg                                                                                                                       >  2:29534/1‑300 (MQ=255)                                                                                                                                                                               tCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCa                                                                                                                     <  2:193891/300‑1 (MQ=255)                                                                                                                                                                                    ccGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGc                                                                                                                <  1:143982/300‑1 (MQ=255)                                                                                                                                                                                         gCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGACGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCATGCTGCGCGCGGCAATGTCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCAACTACCTACATTACTATCGCTCCTTCAAAACTGCGTACGAAATGGCCTGTATACGTGCAGCGCAGCAAATGGCATTCAACGGTCCATGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAgct                                                                                                           >  2:69548/1‑298 (MQ=255)                                                                                                                                                                                                   tttCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCaa                                                                                                  >  1:624647/1‑300 (MQ=255)                                                                                                                                                                                                    ttCTGGACTTA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTACAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATGACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAAc                                                                                                 <  1:210263/300‑1 (MQ=255)                                                                                                                                                                                                     tCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCNAAAGGGGTTATCGACTACCTGCATTANTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACa                                                                                                <  2:471781/300‑1 (MQ=255)                                                                                                                                                                                                              a‑aGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATTGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATc                                                                                       <  1:152569/300‑1 (MQ=255)                                                                                                                                                                                                                 gATGTGTAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTg                                                                                     <  1:86784/300‑1 (MQ=255)                                                                                                                                                                                                                  aTGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGa                                                                                    >  1:203340/1‑300 (MQ=255)                                                                                                                                                                                                                    gtgGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGAcc                                                                                  >  1:28365/1‑300 (MQ=255)                                                                                                                                                                                                                                        gAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATAc                                                              <  1:58029/300‑1 (MQ=255)                                                                                                                                                                                                                                             ccGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCTTGATACCGACg                                                         <  1:353091/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aTTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTAc                                                 <  2:19269/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ctgctgcctgctgcGCGCGGCAATATCGGTTATATCGGTCCGATGCAGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCNCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCAGTTCTGGCATGAGCGAGTTCGATATCAACATTGACTATCTGACCGCGAACGGTCATCGTGATACCGACGTACCTTACAGCAACAtt                                        >  2:159629/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  tgcctgctgcGCGCGGCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCNGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGNCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTgg                                    <  2:637250/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                ggCAATATCGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAacac                      <  1:421728/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGt              <  1:233951/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cGGTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGAAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAANCTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCAGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGt              <  1:491999/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gTTATATCGGTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTAc            <  1:71097/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ggTCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTac    <  2:262535/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    tCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGNCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCNGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTacac  <  2:547390/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    tCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTacac  <  2:672476/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    tCCGATGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGAGCTTAACGAACACGCTGCGGTACTGCATTacac  <  1:690091/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        ACTCCGGCGAGCTGTTCAATGTTTTTCTCGACGATCATCCCTATCCGTTTAAAGTGAACCCGCAATTCAAAGCGTGGGTGCCGGTAACTCAGGTGCCAAACTGCTGGTTGCTGGTGGATGGCGTGAACAAGCCGAAACTGTGGTTCTATCTGCCGGTTGATTACTGGCACAACGTCGAACCGCTGCCGAC‑CTCTTTCTGGACTGA‑AGATGTGGAAGTGATCGCGCTGCCGAAAGCCGATGGCATTGGTAGCCTGCTGCCTGCTGCGCGCGGCAATATCGGTTATATCGGTCCGGTGCCGGAACGTGCGCTGCAACTGGGTATTGAGGCCAGCAACATCAACCCGAAAGGGGTTATCGACTACCTGCATTACTATCGCTCCTTCAAAACTGAGTACGAACTGGCCTGTATGCGTGAAGCGCAGAAAATGGCGGTCAACGGTCACCGCGCGGCAGAAGAAGCGTTCCGTTCTGGCATGAGCGAGTTCGATATCAACATTGCCTATCTGACCGCGACCGGTCATCGTGATACCGACGTACCTTACAGCAACATTGTGGCGCTTAACGAACACGCTGCGGTACTGCATTACAC  >  NC_000913/4031261‑4031849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑