Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,155	G→A	G431E (GGG→GAG)	recQ →	ATP‑dependent DNA helicase RecQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,155	0	G	A	100.0%	324.4 / NA	93	G431E (GGG→GAG)	recQ	ATP‑dependent DNA helicase RecQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/50);  total (43/50)

GCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC  >  NC_000913/4006859‑4007450                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        gCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGAt                                                                                                                                                                                                                                                                                                      <  1:168580/300‑1 (MQ=255) gCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGCTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGAt                                                                                                                                                                                                                                                                                                      <  2:414896/300‑1 (MQ=255)     gcgGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCTAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGt                                                                                                                                                                                                                                                                                                  <  2:296551/300‑1 (MQ=255)     gcgGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGt                                                                                                                                                                                                                                                                                                  >  1:450887/1‑300 (MQ=255)         aaGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTAtg                                                                                                                                                                                                                                                                                              <  1:588337/300‑1 (MQ=255)          aGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCTAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTAtgt                                                                                                                                                                                                                                                                                             <  1:202571/300‑1 (MQ=255)          aGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTAtgt                                                                                                                                                                                                                                                                                             <  2:400022/300‑1 (MQ=255)          aGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTAtgt                                                                                                                                                                                                                                                                                             <  1:455947/300‑1 (MQ=255)          aGCGATGCTGGTGTACGATCCTGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAATAGAAGCCGCAGTGGCAGTGGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGTAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGATTTGAGATGGGTTAtgc                                                                                                                                                                                                                                                                                             <  2:137333/300‑2 (MQ=255)            tgATGCTGTTTGACGATCCGGCTGATATGTCGTGGCTGCGCCGTTGTCTGGAAGAGAAGTCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGTGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATgtgg                                                                                                                                                                                                                                                                                           <  2:279858/299‑1 (MQ=255)            cGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTNCTGAACTATTTTGGCGAAGGGCGTCANGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATgtgg                                                                                                                                                                                                                                                                                           <  2:451129/300‑1 (MQ=255)            cGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATgtgg                                                                                                                                                                                                                                                                                           <  1:408311/300‑1 (MQ=255)                gCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGa                                                                                                                                                                                                                                                                                       >  1:421188/1‑300 (MQ=255)                   gTTTTACGATCCGGCTGATATGGCGTGGCTGCGACGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAAACGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGt                                                                                                                                                                                                                                                                                    >  2:15270/1‑300 (MQ=255)                            tCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCgtgg                                                                                                                                                                                                                                                                           <  1:59163/300‑1 (MQ=255)                                 cTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCta                                                                                                                                                                                                                                                                      <  1:218668/300‑1 (MQ=255)                                       tGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGTGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAAACGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCcaataaac                                                                                                                                                                                                                                                                >  2:330729/1‑298 (MQ=255)                                       tGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCTAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAAcc                                                                                                                                                                                                                                                                <  1:159694/300‑1 (MQ=255)                                       tGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAAcc                                                                                                                                                                                                                                                                <  1:115009/300‑1 (MQ=255)                                       tGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATANCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAAcc                                                                                                                                                                                                                                                                <  2:517794/300‑1 (MQ=255)                                       tGGCGTGGATGCGCCGTTGTCTGTAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCTCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTTGCGAAGGGCGTCAGTAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATGCGTGGTGCTAATAAcc                                                                                                                                                                                                                                                                <  1:256577/300‑1 (MQ=255)                                                         gTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATNCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCCGCGTATCCGCGACTATg                                                                                                                                                                                                                                              >  2:600204/1‑300 (MQ=255)                                                         gTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATg                                                                                                                                                                                                                                              >  1:523409/1‑300 (MQ=255)                                                          tCTGTAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCTAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATgg                                                                                                                                                                                                                                             <  2:357227/300‑1 (MQ=255)                                                          tCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATgg                                                                                                                                                                                                                                             <  1:184385/300‑1 (MQ=255)                                                                 gataaGCCGCATGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATTCGGTGGGCGCATTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATGGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGAc                                                                                                                                                                                                                                      <  2:297134/297‑1 (MQ=255)                                                                 gagaAGNCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGNGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGAc                                                                                                                                                                                                                                      <  2:563528/300‑1 (MQ=255)                                                                 gagaAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGAc                                                                                                                                                                                                                                      <  1:691646/300‑1 (MQ=255)                                                                       ccGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTg                                                                                                                                                                                                                                >  2:119437/1‑300 (MQ=255)                                                                             gggCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTc                                                                                                                                                                                                                          >  1:49240/1‑300 (MQ=255)                                                                                  gTTGCAGGATATCGCGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGCTGCTCCGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGGTGGGTTATGTGGTGGAAGTGATTCGTGNTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATgg                                                                                                                                                                                                                     >  2:519387/1‑300 (MQ=255)                                                                                   ttGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGc                                                                                                                                                                                                                    <  1:604755/300‑1 (MQ=255)                                                                                        ggATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGCCAAACTGAAAGTCTATGGCCTggg                                                                                                                                                                                                               >  2:418703/1‑300 (MQ=255)                                                                                         gATATCGAGCGCCACAAACTCAGTGCGATGGGCGCATTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGGACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCGGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCGCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTGTGGTATGGGc                                                                                                                                                                                                              <  2:530875/300‑1 (MQ=255)                                                                                            atCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTAAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGt                                                                                                                                                                                                           >  2:444939/1‑300 (MQ=255)                                                                                                    ccACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGNGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAg                                                                                                                                                                                                   >  1:492163/1‑300 (MQ=255)                                                                                                     cacaAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGc                                                                                                                                                                                                  <  1:101145/300‑1 (MQ=255)                                                                                                         aaCTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCNTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGCAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGAATGGGCCGTGATAAAAGCCATg                                                                                                                                                                                              >  2:157967/1‑300 (MQ=255)                                                                                                          aCTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGa                                                                                                                                                                                             >  1:657984/1‑300 (MQ=255)                                                                                                            tCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCTTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAAc                                                                                                                                                                                           <  2:7418/300‑1 (MQ=255)                                                                                                            tCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGTTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCTTGATAAAAGCCATGAAc                                                                                                                                                                                           <  2:669802/300‑1 (MQ=255)                                                                                                                  cGATGGGCGCGTTTGCCTAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCNCTTTCCACCATTGGTCGTGTGAATCANCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTggg                                                                                                                                                                                     <  2:472469/300‑1 (MQ=255)                                                                                                                    atgtgcgcGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTg                                                                                                                                                                                   <  2:530368/296‑1 (MQ=255)                                                                                                                        gcgcGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCg                                                                                                                                                                               <  2:223882/300‑1 (MQ=255)                                                                                                                             tttGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATc                                                                                                                                                                          <  1:15483/300‑1 (MQ=255)                                                                                                                                 ccGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGcc                                                                                                                                                                      <  1:544638/300‑1 (MQ=255)                                                                                                                                     aGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCt                                                                                                                                                                  <  1:445532/300‑1 (MQ=255)                                                                                                                                      gcgcAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTg                                                                                                                                                                 >  2:122394/1‑300 (MQ=255)                                                                                                                                          aaaCTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTc                                                                                                                                                             <  1:283978/300‑1 (MQ=255)                                                                                                                                                   gtcgtcTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGcc                                                                                                                                                    <  1:326705/300‑1 (MQ=255)                                                                                                                                                    tcgtcgGGTATTGCTGAACTATTTTGGCGAAGGGCGTCATGAGCCGTGCGGGAACTGCGATATCGGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATTGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCt                                                                                                                                                   <  2:237555/300‑1 (MQ=255)                                                                                                                                                         tGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGa                                                                                                                                              <  2:410380/300‑1 (MQ=255)                                                                                                                                                           gTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCAGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGACAGCTGATTCACCTCGGCCTGGTGACg                                                                                                                                            >  1:241473/1‑300 (MQ=255)                                                                                                                                                                      cTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGGGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGc                                                                                                                                 <  1:572101/300‑1 (MQ=255)                                                                                                                                                                                    ggCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGccc                                                                                                                   >  1:695232/1‑300 (MQ=255)                                                                                                                                                                                       ggcagtaGCCGTGCGGGAACTTCGCTAGCTGCCTCGATCCGCCGAACCAGTACGACGGTCCAACCGATGCTCAGATCGTCCTTTCCACCATTGTTCGTGTGAATTAGCGGTGTTAGATGGTTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCTTGATAAAAGCCATGAACATTGAGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACTCAAAATACTGCCCAGCATTCTGCCCTAc                                                                                                                <  2:363479/294‑1 (MQ=255)                                                                                                                                                                                       gTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGGCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTAc                                                                                                                >  2:594700/1‑300 (MQ=255)                                                                                                                                                                                       gTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTAGGCCTGGTGACGCAAAATATTGCACAGCATTCTGCCCTAc                                                                                                                >  2:236605/1‑300 (MQ=255)                                                                                                                                                                                       gTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAATACGACNGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATTAACATTGGGTGAGCGTGATCCGCNAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTAc                                                                                                                >  2:644091/1‑300 (MQ=255)                                                                                                                                                                                         cAGGAGCCGTGCGGGAACTGCGATATCTGCCTCTATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACaa                                                                                                              >  2:620873/1‑300 (MQ=255)                                                                                                                                                                                         cAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACaa                                                                                                              <  1:664575/300‑1 (MQ=255)                                                                                                                                                                                             aGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTACAACATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTACTAACCAGCGTATCCGCGACTATGGTCATGACAAAATGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATAAACATTGGGTGAGCGTGATCCGCCGGCTGATTCACCTCGGCCTGGTGACGCAAAACATTGCCCAGCATTCTGCCCtccacctac                                                                                                          >  2:303493/1‑292 (MQ=255)                                                                                                                                                                                                    cGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTNATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGTATTCTGCCCTACAACTGACAGAGGc                                                                                                   <  1:500904/300‑1 (MQ=255)                                                                                                                                                                                                           tGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCgcgcgc                                                                                            >  1:671538/1‑300 (MQ=255)                                                                                                                                                                                                                  tCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGtgc                                                                                     <  1:212500/300‑1 (MQ=255)                                                                                                                                                                                                                       cTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCGTTTCCACCATTGTTCGTGTGAATCAGCGGTTTTAGATGGGTTATGTGGTGGAAGTGATTCGTGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGNCAAACTGAAAGTCTATGGCATGGGCCNTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgc                                                                                <  2:452310/300‑1 (MQ=255)                                                                                                                                                                                                                       cTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgc                                                                                <  1:205266/300‑1 (MQ=255)                                                                                                                                                                                                                             ccgccgAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGNCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCAGTGCTGCGCGGCGaa                                                                          >  2:161668/1‑300 (MQ=255)                                                                                                                                                                                                                              cgccgAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCACTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAAt                                                                         >  1:570982/1‑300 (MQ=255)                                                                                                                                                                                                                                      cAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCCGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTg                                                                 >  2:621291/1‑300 (MQ=255)                                                                                                                                                                                                                                        gTACGACGGTTCAACCGATGCTCAGATTGCACTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGAAAAATATTGCCCAGCATTCTGCACTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCa                                                               >  1:179606/1‑300 (MQ=255)                                                                                                                                                                                                                                               ggTTCAGCCGATGCTCAGATTGCCCTTTCCACCATGGTTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGTTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGANTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGNGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                        <  2:553323/300‑1 (MQ=255)                                                                                                                                                                                                                                               ggTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                        >  1:92997/1‑300 (MQ=255)                                                                                                                                                                                                                                                gTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCANCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                       >  1:494215/1‑300 (MQ=255)                                                                                                                                                                                                                                                      ccGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCcgc                                                 >  2:626625/1‑300 (MQ=255)                                                                                                                                                                                                                                                      ccGATGCTCAGATTGCCCTTTACACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCcgc                                                 >  2:418616/1‑300 (MQ=255)                                                                                                                                                                                                                                                               aGATTGCCCTTTCCACCATTGTTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTGGCAACTTGCCGTGCCGCGTATCGTgg                                        <  1:572065/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCACGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                       >  2:349790/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     cccTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGTGCTCa                                  >  2:229347/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                >  1:453786/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ttCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCACGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaaac                              >  2:74937/1‑298 (MQ=255)                                                                                                                                                                                                                                                                         ttCCACCATTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTAAAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCAGTGCCGCGTATCGTGGCGCTCAAAcc                              >  1:128676/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             accaTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAANTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                          >  1:469193/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             accaTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                          >  2:57255/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             accaTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTAAAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                          >  1:286034/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ccaTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                         >  2:332216/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ccaTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTANGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                         <  1:508382/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               caTTGGTCGTGTGAATCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                        <  1:543724/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gtgAATCAGCGGTTTGAGATGTGTTATGTGGTGGAAGTGATTCGTGGTGCGAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGATGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa              <  2:257498/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gtgAATCAGCGCTTTGATATGGGTTATGTGGTGGAAGTGGTTCGTGGGGCTAATAACCAGCGTATCCGCCACTATGTTCATAACAAACTGAAAGTCTATTGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCACTCAAACCGAAAGCGATGCAGaaa              <  2:231415/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aaTCAGCGGTTTGAGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTCTGCCACTTGCCGTGCCGCGTATCGTGGCGCTCAAACAAAAAACGATGCAGAAATca           >  2:141660/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                    gTTTGAGATGGGTTATGTGGTGGAAGTGATTNGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGNGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg   >  2:491851/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     tttGAGATGGGTTATGTGGTGGAAGTGATTCNTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGANCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCggcggc  >  2:485624/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        GCCCGCGGAAGCGATGCTGTTTTACGATCCGGCTGATATGGCGTGGCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAGCGCCACAAACTCAATGCGATGGGCGCGTTTGCCGAAGCGCAAACTTGCCGTCGTCTGGTATTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGCGATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAATCAGCGGTTTGGGATGGGTTATGTGGTGGAAGTGATTCGTGGTGCTAATAACCAGCGTATCCGCGACTATGGTCATGACAAACTGAAAGTCTATGGCATGGGCCGTGATAAAAGCCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC  >  NC_000913/4006859‑4007450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑