Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,803	G→A	T86I (ACC→ATC)	cyaY ←	frataxin CyaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,803	0	G	A	98.9%	311.3 / NA	90	T86I (ACC→ATC)	cyaY	frataxin CyaY
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (30/59);  total (31/59)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGGTTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTAT  >  NC_000913/3993507‑3994094                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                    cTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGAttt                                                                                                                                                                                                                                                                                                  >  1:87087/1‑300 (MQ=255) cTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGAttt                                                                                                                                                                                                                                                                                                  >  2:361675/1‑300 (MQ=255)    cTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTcgc                                                                                                                                                                                                                                                                                               >  1:331226/1‑300 (MQ=255)                    gACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTANCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAAt                                                                                                                                                                                                                                                                               <  1:473840/300‑1 (MQ=255)                      ccGTTTTACCTACTGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATcc                                                                                                                                                                                                                                                                             <  2:262308/300‑1 (MQ=255)                       cGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGNCAATCAGGATCACGATACGCCGCTATNACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCAAGAAGATTTCGCCGCTGCGATCACAAATCCa                                                                                                                                                                                                                                                                            >  2:455692/1‑300 (MQ=255)                       cGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCa                                                                                                                                                                                                                                                                            >  1:295154/1‑300 (MQ=255)                        gTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCAc                                                                                                                                                                                                                                                                           >  1:113467/1‑300 (MQ=255)                        gTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCAc                                                                                                                                                                                                                                                                           <  2:522319/300‑1 (MQ=255)                        gTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGANTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCNTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCAc                                                                                                                                                                                                                                                                           <  2:642914/300‑1 (MQ=255)                             aCCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATc                                                                                                                                                                                                                                                                      >  1:537200/1‑300 (MQ=255)                                   ggCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCttt                                                                                                                                                                                                                                                                >  1:672044/1‑300 (MQ=255)                                    gCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGNTGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTNCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTc                                                                                                                                                                                                                                                               <  2:635574/300‑1 (MQ=255)                                    gCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTc                                                                                                                                                                                                                                                               <  2:189697/300‑1 (MQ=255)                                    gCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTc                                                                                                                                                                                                                                                               <  1:254223/300‑1 (MQ=255)                                    gCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCACCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTc                                                                                                                                                                                                                                                               <  2:318240/300‑1 (MQ=255)                                       cAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAgg                                                                                                                                                                                                                                                            <  2:223817/300‑1 (MQ=255)                                              catcaACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAAt                                                                                                                                                                                                                                                     <  2:614916/300‑1 (MQ=255)                                              catcaACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGANGAACGTGCCGGAAAGCGAGGCTTATCNGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAAt                                                                                                                                                                                                                                                     <  2:458581/300‑1 (MQ=255)                                                   aCTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCTTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAg                                                                                                                                                                                                                                                >  2:311869/1‑300 (MQ=255)                                                   aCTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAg                                                                                                                                                                                                                                                >  1:385402/1‑300 (MQ=255)                                                    cTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAgc                                                                                                                                                                                                                                               <  2:21261/300‑1 (MQ=255)                                                                 cAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGt                                                                                                                                                                                                                                  <  1:545144/300‑1 (MQ=255)                                                                 cAGTTCTATCAGATTGGGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGTTCAAAATGGTAGCCGCCCTGTTTGGt                                                                                                                                                                                                                                  <  2:59901/300‑1 (MQ=255)                                                                   tttCTATTAGATTTTGAGGGTTGATGGTCGTGAACAGTTGATTCCGTTCCACACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATGACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGACTTCGCTGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTg                                                                                                                                                                                                                                <  2:121527/299‑1 (MQ=255)                                                                   gTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACNAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCNATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTg                                                                                                                                                                                                                                <  2:645378/300‑1 (MQ=255)                                                                   gTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCNGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTg                                                                                                                                                                                                                                >  1:462373/1‑300 (MQ=255)                                                                   gTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTg                                                                                                                                                                                                                                <  2:126077/300‑1 (MQ=255)                                                                   gTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTg                                                                                                                                                                                                                                <  1:671715/300‑1 (MQ=255)                                                                   gTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTAATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTg                                                                                                                                                                                                                                <  1:264748/300‑1 (MQ=255)                                                                      cTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTgcca                                                                                                                                                                                                                             >  1:415088/1‑300 (MQ=255)                                                                       gcgagatgtgtataagagacaggTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTgccag                                                                                                                                                                                                                            >  1:575434/22‑300 (MQ=255)                                                                        aTCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTgccagc                                                                                                                                                                                                                           >  1:655625/1‑300 (MQ=255)                                                                          cAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTgccagcca                                                                                                                                                                                                                         <  1:341363/300‑1 (MQ=255)                                                                              ttGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATAcc                                                                                                                                                                                                                     >  1:187590/1‑300 (MQ=255)                                                                               tgtgAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCTTGATGAACGTGCCGGAAAGCTAAGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCt                                                                                                                                                                                                                    <  1:91131/300‑1 (MQ=255)                                                                               tgtgAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCTTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCt                                                                                                                                                                                                                    <  2:676069/300‑1 (MQ=255)                                                                               tgtgAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCt                                                                                                                                                                                                                    <  2:245125/300‑1 (MQ=255)                                                                               tgtgAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCt                                                                                                                                                                                                                    <  1:220644/300‑1 (MQ=255)                                                                               tgtgAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTNCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCt                                                                                                                                                                                                                    <  1:463991/300‑1 (MQ=255)                                                                               tgtgAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCCATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCTTNATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCNCCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCt                                                                                                                                                                                                                    <  2:546997/300‑1 (MQ=255)                                                                                    aGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGc                                                                                                                                                                                                               <  1:334491/300‑1 (MQ=255)                                                                                    aGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCGATCGGTAACANGCCGCCTGCCAATCAGGATCACGAGACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGc                                                                                                                                                                                                               <  2:517010/300‑1 (MQ=255)                                                                                     ggTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCa                                                                                                                                                                                                              >  1:689682/1‑300 (MQ=255)                                                                                      gTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAg                                                                                                                                                                                                             >  1:524649/1‑300 (MQ=255)                                                                                        tGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCATGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCg                                                                                                                                                                                                           <  2:315617/300‑1 (MQ=255)                                                                                             gTCGTGAACAGGTGATTCCGTTCCGCACAAANTCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTNATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCATTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTcc                                                                                                                                                                                                      >  2:486989/1‑300 (MQ=255)                                                                                                        gTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGAt                                                                                                                                                                                           <  1:265431/300‑1 (MQ=255)                                                                                                           aTTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAAt                                                                                                                                                                                        <  1:313288/300‑1 (MQ=255)                                                                                                           aTTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTACAGCGGCTCCTGGCGGTTGATAAt                                                                                                                                                                                        >  2:618334/1‑300 (MQ=255)                                                                                                             tccgttccgCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGa                                                                                                                                                                                      <  1:204019/300‑1 (MQ=255)                                                                                                                                  cGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTAAAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAgg                                                                                                                                                                 >  2:535205/1‑300 (MQ=255)                                                                                                                                    gTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCCTTCTCAAAGGTa                                                                                                                                                               >  2:29468/1‑300 (MQ=255)                                                                                                                                        cATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGNCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATgg                                                                                                                                                           >  2:598118/1‑300 (MQ=255)                                                                                                                                               ccTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGACCGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTAc                                                                                                                                                    <  2:654293/300‑1 (MQ=255)                                                                                                                                               ccTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTAc                                                                                                                                                    <  1:696656/300‑1 (MQ=255)                                                                                                                                                    cAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTNCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTAcgccgc                                                                                                                                               <  2:162935/300‑1 (MQ=255)                                                                                                                                                    cAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAANATTTCGCCGCTGCGATCACAAATCCANTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTAcgccgc                                                                                                                                               <  2:452409/300‑1 (MQ=255)                                                                                                                                                    cAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTAcgccgc                                                                                                                                               <  1:282789/300‑1 (MQ=255)                                                                                                                                                    cAATCACGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAACATTTCGCCGCTGCGATCACAAATCCANTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTNCCATTCTCAAAGGTAATGGTCAGTAcgccgc                                                                                                                                               <  2:480147/300‑1 (MQ=255)                                                                                                                                                              cACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTc                                                                                                                                     <  2:244723/300‑1 (MQ=255)                                                                                                                                                                cGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGc                                                                                                                                   >  1:650994/1‑300 (MQ=255)                                                                                                                                                                cGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCATTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGc                                                                                                                                   >  2:652685/1‑300 (MQ=255)                                                                                                                                                                          cTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATc                                                                                                                         <  1:342087/300‑1 (MQ=255)                                                                                                                                                                            aTTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGTTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGc                                                                                                                       <  1:220704/300‑1 (MQ=255)                                                                                                                                                                              tACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTg                                                                                                                     <  1:349689/300‑1 (MQ=255)                                                                                                                                                                                                     aCGTGCCGGAAATCGATGCTTATCCGGCATACAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCg                                                                                              <  2:147859/300‑1 (MQ=255)                                                                                                                                                                                                                agctaGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGAAGGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTGTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAACCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCTTTGATTTCGCAGTCGATATCGCCGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATgg                                                                                   <  2:353333/296‑1 (MQ=255)                                                                                                                                                                                                                aGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATgg                                                                                   <  2:526458/300‑1 (MQ=255)                                                                                                                                                                                                                aGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATgg                                                                                   <  1:577549/300‑1 (MQ=255)                                                                                                                                                                                                                     ggCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGANAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGc                                                                              >  1:479204/1‑300 (MQ=255)                                                                                                                                                                                                                           tCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGt                                                                        <  1:593146/300‑1 (MQ=255)                                                                                                                                                                                                                                cATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATc                                                                   <  1:563993/300‑1 (MQ=255)                                                                                                                                                                                                                                cATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTGCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATc                                                                   <  2:373206/300‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCATAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCa                                                              <  2:344485/300‑1 (MQ=255)                                                                                                                                                                                                                                                aaaCTAACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCAAGAAGGTTTAGACGCTGCGATCACAAATCCACTCATCGCCTTTAAGGTCAAAATGGTAGCCGCACTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATNATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTATCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAAt                                                   >  2:153501/1‑300 (MQ=255)                                                                                                                                                                                                                                                  actgactgTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCAATCACAAATCCACTCATTTCCTTTCAGGTCAAAATGGTAGCCGCCCTTTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATAATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTc                                                 <  2:150324/300‑1 (MQ=255)                                                                                                                                                                                                                                                       ctgTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTNTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCANAGTTGATCAGCCAGGCGATGAAATTCACTGt                                            <  2:491749/300‑1 (MQ=255)                                                                                                                                                                                                                                                       ctgTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGt                                            <  1:71967/300‑1 (MQ=255)                                                                                                                                                                                                                                                       ctgTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATGTCGACGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGt                                            <  2:395060/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGAGATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCg                                          >  2:416407/1‑300 (MQ=255)                                                                                                                                                                                                                                                            tCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCCGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTc                                       >  1:352885/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCa                                      >  1:667163/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCGGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCAttgtt                                 >  2:204304/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gctgcGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGTTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTAt                             <  2:327528/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tgcGTCGTCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGNGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATcc                           <  2:164488/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tgcGTCGCCGCCTGTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATcc                           <  2:648580/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tgcGTCGCCGCCTGTTCCAACAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATTGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATcc                           <  2:92400/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gTTCCAGCAAATCCCAGAAGATTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGa              >  2:526116/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 cccAGAAGATTTCGCCGCTGCGATCACAAATCCACTCACCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCCTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGtat  <  2:86772/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                    CTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTCGCCGCCTGTTCCAGCAAATCCCAGAAGGTTTCGCCGCTGCGATCACAAATCCACTCATCGCCTTTCAGGTCAAAATGGTAGCCGCCCTGTTTGGTTGCCAGCCATACCTGGTGCAGCGGCTCCTGGCGGTTGATAATGATTTTGCTGCCATTCTCAAAGGTAATGGTCAGTACGCCGCCGTTGATTTCGCAGTCGATATCGCTGTCGCCATCCCAGTCGTCCAGGCGTTCTTCAATGGTCAGCCAGAGTTGATCAGCCAGGCGATGAAATTCACTGTCGTTCATTGTTGTATCCTGTTTTTAAGTGATGGCGGCAGTAT  >  NC_000913/3993507‑3994094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑