Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,264	G→A	E544E (GAG→GAA)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,264	0	G	A	100.0%	328.9 / NA	95	E544E (GAG→GAA)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/45);  total (50/45)

CTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTC  >  NC_000913/3223968‑3224555                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    cTGGGCAATGAATCCGGCTATTGCTGTAACATCCGCGCGATGTACCATGCTGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAatgg                                                                                                                                                                                                                                                                                                  <  1:272319/300‑1 (MQ=255)  tGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAatggt                                                                                                                                                                                                                                                                                                 <  2:366555/300‑1 (MQ=255)   gggCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAatggtg                                                                                                                                                                                                                                                                                                <  1:592803/300‑1 (MQ=255)       aaTGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGAc                                                                                                                                                                                                                                                                                            <  1:603393/300‑1 (MQ=255)         tGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCTCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCATGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCa                                                                                                                                                                                                                                                                                          <  2:116247/300‑1 (MQ=255)         tGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCa                                                                                                                                                                                                                                                                                          <  2:116103/300‑1 (MQ=255)          gAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGNATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCAc                                                                                                                                                                                                                                                                                         >  1:475416/1‑300 (MQ=255)            aTCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACgg                                                                                                                                                                                                                                                                                       >  2:243659/1‑300 (MQ=255)                ggCTATGGCTGTACCATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGTGAACACGGGATc                                                                                                                                                                                                                                                                                   >  2:103012/1‑300 (MQ=255)                ggCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCCGGGTCATTATGTCTGGGAATGGTGCGAACACGGGATc                                                                                                                                                                                                                                                                                   >  2:203715/1‑300 (MQ=255)                ggCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGAGCTGACG‑GAGTACCAGAACGTCTTCTATACGCACGATTGCCTTCAGGGTCGTTATGTCTGGGAATGGTGCGaacccgagatc                                                                                                                                                                                                                                                                                   >  2:24730/1‑290 (MQ=255)                    aTGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGCAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTATATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAgg                                                                                                                                                                                                                                                                               >  2:287775/1‑300 (MQ=255)                     tGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGc                                                                                                                                                                                                                                                                              <  1:25998/300‑1 (MQ=255)                      ggCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGNGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGca                                                                                                                                                                                                                                                                             >  1:480945/1‑300 (MQ=255)                      ggCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGca                                                                                                                                                                                                                                                                             >  1:73550/1‑300 (MQ=255)                       gCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACTAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCATGcac                                                                                                                                                                                                                                                                            <  2:450433/300‑1 (MQ=255)                       gCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGcac                                                                                                                                                                                                                                                                            <  1:90364/300‑1 (MQ=255)                       gCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGAACGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCCACAAGCACGATCGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGacac                                                                                                                                                                                                                                                                            >  2:105512/1‑300 (MQ=255)                            aaCATCCGCGCGATGTACCAGGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGNTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCAGGATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGgac                                                                                                                                                                                                                                                                       <  2:163846/300‑1 (MQ=255)                                 gagacagATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCa                                                                                                                                                                                                                                                                  <  2:656370/294‑1 (MQ=255)                                        aTGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCGCGGCAAt                                                                                                                                                                                                                                                           >  2:240489/1‑300 (MQ=255)                                        aTGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCActgtctc                                                                                                                                                                                                                                                           >  1:671752/1‑294 (MQ=255)                                        aTGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAAt                                                                                                                                                                                                                                                           >  1:402577/1‑300 (MQ=255)                                          gTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GCGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGCCCACGGCAATGt                                                                                                                                                                                                                                                         >  2:328502/1‑300 (MQ=255)                                          gTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGt                                                                                                                                                                                                                                                         >  1:165875/1‑300 (MQ=255)                                                 acggtgAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTTAAGTGGTCGATGTTGTTTCCACCATGCACACCCACATGCCGCTGATGAATGAGGTTGGTGAATACCCGCATCCGAAGCCGCGCATCACCTGTGAATCTGCTCATGCGATGGGGAACGGACCGTTCGGGCTAACGTGA‑TACCAGCACGTCTCCTATAAACACGATTGCATTCAGGGGCGTTATGTCTGGGAATAGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGtat                                                                                                                                                                                                                                                  <  2:51349/295‑1 (MQ=255)                                                                        gcgACTGTTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCATGTTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcga                                                                                                                                                                                                                           <  2:59957/300‑1 (MQ=255)                                                                         cgACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcgac                                                                                                                                                                                                                          >  1:442125/1‑300 (MQ=255)                                                                         cgACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcgac                                                                                                                                                                                                                          >  2:666750/1‑300 (MQ=255)                                                                            cTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCAAGGCACAGGACGACCACGGCAATGTCTAGTATAAATTCGGCGGTGACTACGGCGATTAt                                                                                                                                                                                                                       >  2:177499/1‑300 (MQ=255)                                                                                          agaagaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGAGGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATaa                                                                                                                                                                                                         <  1:101892/300‑1 (MQ=255)                                                                                          agaagaTCGCGATGCTGAAGTGGTCGATATGATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATaa                                                                                                                                                                                                         <  2:437908/300‑1 (MQ=255)                                                                                            aagaTTGCGATGCTTAAGTGGTCGATATTATTTCCACCATGTACACCCACGTGCCGCTGATGAATTAGTTGGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACt                                                                                                                                                                                                       <  2:83131/300‑1 (MQ=255)                                                                                            aagaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACt                                                                                                                                                                                                       <  1:45989/300‑1 (MQ=255)                                                                                              gaTCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGcggcgactacggcctgtctcttatacacatctcgcag                                                                                                                                                                                                     <  1:201346/300‑25 (MQ=255)                                                                                                     aTGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTg                                                                                                                                                                                              >  1:372381/1‑300 (MQ=255)                                                                                                          gAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGt                                                                                                                                                                                         >  1:568092/1‑300 (MQ=255)                                                                                                                gTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATc                                                                                                                                                                                   <  1:362737/300‑1 (MQ=255)                                                                                                                     tattatTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATANCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGNGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTc                                                                                                                                                                              <  2:645733/300‑1 (MQ=255)                                                                                                                     tattatTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACTGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTc                                                                                                                                                                              <  2:99143/300‑1 (MQ=255)                                                                                                                     tattatTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTc                                                                                                                                                                              <  1:67605/300‑1 (MQ=255)                                                                                                                     tatgattTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAATGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTc                                                                                                                                                                              <  2:283164/296‑1 (MQ=255)                                                                                                                          tttCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATc                                                                                                                                                                         <  1:37038/300‑1 (MQ=255)                                                                                                                               accaTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGAACCGGGCGGGCTGACA‑GAGTACCAGAACGTCTTCTATAAGTACGATTGCGTTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCCCAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCAAACAACTATAACTTCTGTCTTGGTGGTTTGATCTATTCCGATCGGACg                                                                                                                                                                    >  2:133738/1‑300 (MQ=255)                                                                                                                                  aTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCg                                                                                                                                                                 >  1:81293/1‑300 (MQ=255)                                                                                                                                  aTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCg                                                                                                                                                                 >  2:262773/1‑300 (MQ=255)                                                                                                                                  aTGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACAACCACGGCAATGTCTGGTATCAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCg                                                                                                                                                                 >  2:63491/1‑300 (MQ=255)                                                                                                                                    gTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATGAATTCGGCGGCGACTACGGCGACTATCNCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCggg                                                                                                                                                               >  2:504779/1‑300 (MQ=255)                                                                                                                                                gCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGaa                                                                                                                                                   <  2:396107/300‑1 (MQ=255)                                                                                                                                                  cGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGTGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAag                                                                                                                                                 <  2:284444/300‑1 (MQ=255)                                                                                                                                                  cGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAag                                                                                                                                                 <  1:673659/300‑1 (MQ=255)                                                                                                                                                           atgaGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATACTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAAc                                                                                                                                        <  2:525754/300‑1 (MQ=255)                                                                                                                                                            tgaGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCANGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTNGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACa                                                                                                                                       <  2:640679/300‑1 (MQ=255)                                                                                                                                                             gaGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAg                                                                                                                                      >  1:14959/1‑300 (MQ=255)                                                                                                                                                               gTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTCTAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGt                                                                                                                                    >  2:425389/1‑300 (MQ=255)                                                                                                                                                                    gTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATcg                                                                                                                               >  1:37160/1‑300 (MQ=255)                                                                                                                                                                           cccGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGt                                                                                                                        >  1:206262/1‑300 (MQ=255)                                                                                                                                                                                  ccGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATc                                                                                                                 <  1:64917/300‑1 (MQ=255)                                                                                                                                                                                        ccGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCNCGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCNACTATCCCAACAACTATAAATTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCAcgcg                                                                                                           >  2:547431/1‑300 (MQ=255)                                                                                                                                                                                           cgcATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAANAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAACCAGGTTATCGCGCCGGTAAAAATCCACGCGCgg                                                                                                        >  2:609189/1‑300 (MQ=255)                                                                                                                                                                                               tcatcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATc                                                                                                    <  1:448730/300‑1 (MQ=255)                                                                                                                                                                                                 atcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTg                                                                                                  >  1:94160/1‑300 (MQ=255)                                                                                                                                                                                                  tcTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCANGCACAGGACGACCACGGCAATGTCTGNTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGa                                                                                                 >  2:456940/1‑300 (MQ=255)                                                                                                                                                                                                                     gATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGt                                                                              >  1:239688/1‑300 (MQ=255)                                                                                                                                                                                                                        ggggAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGCTCTGACGCGCGGCGAGTTGAAAGTCGa                                                                           >  2:243225/1‑300 (MQ=255)                                                                                                                                                                                                                        ggggAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGa                                                                           >  2:408259/1‑300 (MQ=255)                                                                                                                                                                                                                                  ccGGGCGGGCTTACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCNGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAAcgg                                                                 <  2:170433/300‑3 (MQ=255)                                                                                                                                                                                                                                  ccGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACtg                                                                 <  2:667335/300‑1 (MQ=255)                                                                                                                                                                                                                                         ggcTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTa                                                          >  1:353496/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTNCGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTAATCTATTCCGATCAGACGNCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAAATGTGGTTTACCAAGCtt                                                  >  2:539767/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGCCGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCtt                                                  >  1:583053/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCAAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGNGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCtt                                                  >  2:517523/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gAGTACCAGAACGTCTTATATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCAAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGAAGCCGGGCCCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCtt                                                  >  2:121584/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGCCCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCAGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtga                                                >  1:140826/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtga                                                >  1:532937/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCAAACAACTATAACTTCTGTATTGATGGTTTGANCTATTCCGATCAGACGCAGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTtga                                                >  2:156892/1‑300 (MQ=255)                                                                                                                                                                                                                                                        cAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACTGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGAc                                            <  2:584964/300‑1 (MQ=255)                                                                                                                                                                                                                                                        cAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGNCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGAc                                            <  1:472546/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCNACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTacac                                       <  1:469396/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGTGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTacac                                       <  2:42580/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTacac                                       <  2:410605/300‑1 (MQ=255)                                                                                                                                                                                                                                                             cGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTacac                                       <  1:141760/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tcttctNTAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCNCGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGACGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACAccc                                     <  2:572052/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tcttctATAAGCACGATTGCATTCATGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACAccc                                     <  2:302621/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tcttctATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACAccc                                     <  1:245525/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   ctATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCa                                 >  1:456056/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      taAGCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGc                              <  1:266970/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gctcggagatgtgtataagagacaggTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCaga                           <  1:156374/275‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCACGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCaga                           <  2:352782/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           aCGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAgg                         <  1:319406/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cGATTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGt                        >  1:311614/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aTTGCATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCAAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgc                      >  1:451588/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cATTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcgc                  >  1:205160/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   aTTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGcc                 <  1:684583/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aTTCAGGGTCATTATGTCTGGGAATGGTGCGACCACGAGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCACGCCGGCAAAAATCCACGCGCGGGATCTGACTCGCGACGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCCCGCAGAGGTGCGCGcc                 >  2:102113/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              ttATGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAAc      <  1:602650/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 tGTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGCTGAAAGTCGAAAATAAACTGTGGTTTANCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaaaagat   >  2:504118/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                                  gTCTGGGAATGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACANGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCagactgtctcttatacacatctcgcagg  >  2:155501/1‑275 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    CTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCTGAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGTGAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGACCGGGCGGGCTGACG‑GAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCATTATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGGTATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGTTTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTC  >  NC_000913/3223968‑3224555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑