Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,243	C→T	P138S (CCA→TCA)	srlA →	sorbitol‑specific PTS enzyme IIC2 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,243	0	C	T	100.0%	333.4 / NA	95	P138S (CCA→TCA)	srlA	sorbitol‑specific PTS enzyme IIC2 component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (65/30);  total (65/30)

ATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAG  >  NC_000913/2825949‑2826538                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        aTCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACtt                                                                                                                                                                                                                                                                                                    >  1:285087/1‑300 (MQ=255)     tGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGcc                                                                                                                                                                                                                                                                                                >  1:302796/1‑300 (MQ=255)     tGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCNTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGcc                                                                                                                                                                                                                                                                                                >  1:493335/1‑300 (MQ=255)      gAACGCACTGATTAACTTTATCGGTCAGCGTTGTATTGAACTTTTTGCTCAGCGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGTTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCGTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGccc                                                                                                                                                                                                                                                                                               <  2:11452/300‑1 (MQ=255)           cACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTTTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACGACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTgg                                                                                                                                                                                                                                                                                          <  2:61511/300‑1 (MQ=255)               gATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGt                                                                                                                                                                                                                                                                                      >  1:451436/1‑300 (MQ=255)                  tAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGTTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAg                                                                                                                                                                                                                                                                                   <  2:271216/300‑1 (MQ=255)                        tATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATct                                                                                                                                                                                                                                                                             <  1:352141/300‑1 (MQ=255)                         aTCGTTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATctg                                                                                                                                                                                                                                                                            <  2:5166/300‑1 (MQ=255)                          tCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATctgc                                                                                                                                                                                                                                                                           <  1:278957/300‑1 (MQ=255)                                 gCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCtggttgg                                                                                                                                                                                                                                                                    <  2:127923/300‑1 (MQ=255)                                    tCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTtggtct                                                                                                                                                                                                                                                                 <  2:397339/300‑1 (MQ=255)                                      gTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCAATTGGCCCACTGGCGGTGAGTTATCTGCTGGTtggtctgg                                                                                                                                                                                                                                                               >  2:84688/1‑300 (MQ=255)                                             aCGTTTTGCGCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCaa                                                                                                                                                                                                                                                        <  2:443143/300‑1 (MQ=255)                                                    gCTCAACGTTGCGCCGTTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTTGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATttcttc                                                                                                                                                                                                                                                 <  2:182340/300‑1 (MQ=255)                                                    gCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATttcttc                                                                                                                                                                                                                                                 >  2:102485/1‑300 (MQ=255)                                                    gCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATACCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGTCTCTTCCCCCATATCAACCCTGGCGAAATGTTTGTTTATCTTGGCATTGCCAGTGGTCTGACACCGCTGAACCTGTCACTTGGCCCACTGGCGGTAAGTCATCTGCTAGTTGGTCTGGTCACCAATttcttc                                                                                                                                                                                                                                                 >  2:46484/1‑300 (MQ=255)                                                         aCGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCgg                                                                                                                                                                                                                                            >  1:288555/1‑300 (MQ=255)                                                                 ccGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGTCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGa                                                                                                                                                                                                                                    <  1:620690/300‑1 (MQ=255)                                                                  cGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGAc                                                                                                                                                                                                                                   >  1:659289/1‑300 (MQ=255)                                                                            gTTTCCCGTTACCTACTTTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGacc                                                                                                                                                                                                                         <  1:340399/300‑1 (MQ=255)                                                                            gTTTCCCGTTACCTACTTTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTNTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCANTTTCTTCCGCGGCTGGGTGACCGATCTGacc                                                                                                                                                                                                                         <  2:484138/300‑1 (MQ=255)                                                                            gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTTTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGacc                                                                                                                                                                                                                         <  1:540860/300‑1 (MQ=255)                                                                            gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGTTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGacc                                                                                                                                                                                                                         <  2:263908/300‑1 (MQ=255)                                                                            gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGNGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGacc                                                                                                                                                                                                                         <  1:460979/300‑1 (MQ=255)                                                                            gTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGacc                                                                                                                                                                                                                         <  1:338979/300‑1 (MQ=255)                                                                                cccGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTACGCGGCTGGGTGACCGATCTGACCACCg                                                                                                                                                                                                                     >  1:179875/1‑300 (MQ=255)                                                                                 ccGTTACCTACTGTTACCGTGCATTGGCACGTTTATCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTACCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGAACGATCTGACCACCGc                                                                                                                                                                                                                    >  1:46283/1‑300 (MQ=255)                                                                                   gTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCa                                                                                                                                                                                                                  >  1:523393/1‑300 (MQ=255)                                                                                      aCCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCAttt                                                                                                                                                                                                               >  1:561189/1‑300 (MQ=255)                                                                                             gTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGaa                                                                                                                                                                                                        >  2:384375/1‑300 (MQ=255)                                                                                                aCCGTGCATTGGCATGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGTTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGACTGGCCTCTTCCCCACTATCCACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCAACAATTTCTTCCGCGGCTGGGTGACCGATCTGACCCCCGCCATTTTTGAGaaaaa                                                                                                                                                                                                     >  1:241933/1‑300 (MQ=255)                                                                                                                        ttGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACaaaa                                                                                                                                                                             >  1:344625/1‑300 (MQ=255)                                                                                                                              tCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTGTATCTTGGCATTGCCCGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATCCAACTCGACCAACAAGTTCa                                                                                                                                                                       >  2:119052/1‑300 (MQ=255)                                                                                                                                     aCCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCNGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCa                                                                                                                                                                >  1:496336/1‑300 (MQ=255)                                                                                                                                     aCCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACCACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGACCTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGANAACGCTGAACCTGTCACTTGGCCCACNGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCa                                                                                                                                                                <  2:457024/300‑1 (MQ=255)                                                                                                                                      cccTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAg                                                                                                                                                               >  1:260664/1‑300 (MQ=255)                                                                                                                                      cccTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAg                                                                                                                                                               >  2:242978/1‑300 (MQ=255)                                                                                                                                      cccTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAATTCACCTGGCAg                                                                                                                                                               >  2:235194/1‑300 (MQ=255)                                                                                                                                      cccTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTATTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAg                                                                                                                                                               >  2:340391/1‑300 (MQ=255)                                                                                                                                           aGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCATGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCa                                                                                                                                                          >  2:336555/1‑300 (MQ=255)                                                                                                                                            gCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCCGGAGCaa                                                                                                                                                         >  1:254034/1‑300 (MQ=255)                                                                                                                                               tGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTNTAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTNTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAAcat                                                                                                                                                      <  2:637702/300‑1 (MQ=255)                                                                                                                                               tGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAAcat                                                                                                                                                      <  1:405144/300‑1 (MQ=255)                                                                                                                                                gggTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAAcatc                                                                                                                                                     >  1:282028/1‑300 (MQ=255)                                                                                                                                                    cGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGa                                                                                                                                                 <  1:201095/300‑1 (MQ=255)                                                                                                                                                     gCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGAc                                                                                                                                                >  1:312416/1‑300 (MQ=255)                                                                                                                                                     gCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTACCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGAc                                                                                                                                                >  2:280429/1‑300 (MQ=255)                                                                                                                                                             ccGGAAAAGTACAAACCCAGCTACTACGTGGCGGCCTCTTATAGCTGCCACTCAATTAATGGCCTCTTCCTCCATATCAACCGTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTc                                                                                                                                        <  2:66960/300‑1 (MQ=255)                                                                                                                                                                    aGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCTGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGa                                                                                                                                 >  1:630651/1‑300 (MQ=255)                                                                                                                                                                    aGTACAAACCCAGCTACTACGCGGCGGCCTCTCATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCTGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGTAACATCATGACGCATATTCGGATCGa                                                                                                                                 >  1:630782/1‑300 (MQ=255)                                                                                                                                                                     gTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACACAAAATTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGaa                                                                                                                                >  2:126060/1‑300 (MQ=255)                                                                                                                                                                            cccAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGaa                                                                                                                         <  2:389859/300‑1 (MQ=255)                                                                                                                                                                            cccAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGNGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGaa                                                                                                                         >  1:483560/1‑300 (MQ=255)                                                                                                                                                                              cAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACg                                                                                                                       >  2:448410/1‑300 (MQ=255)                                                                                                                                                                                 ctactaCGCGGCGGCCTCTTATAGCTGCCACTCACTGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGt                                                                                                                    >  1:203696/1‑300 (MQ=255)                                                                                                                                                                                   actacGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTgg                                                                                                                  >  2:376920/1‑300 (MQ=255)                                                                                                                                                                                          ggcggcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCCGGCGAACTGTTTGTTTATCTTTGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGggc                                                                                                           <  2:648865/300‑1 (MQ=255)                                                                                                                                                                                              gcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAGCTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACTTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGAACACCGCCATTTTTGAGAAAAAGATGTGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTGGGATCGAAAAAGGAACGGGCGGCTGGGGCGGcc                                                                                                       >  2:303667/1‑300 (MQ=255)                                                                                                                                                                                                ctctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCg                                                                                                     >  1:68624/1‑300 (MQ=255)                                                                                                                                                                                                ctctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCAATTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAGAAGGAACGGGTGGCTGGGGCGGCCCg                                                                                                     >  1:196763/1‑300 (MQ=255)                                                                                                                                                                                                ctctTATAGCTGCCACTCAATGAATGGCCTCNTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGNGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCg                                                                                                     >  2:492812/1‑300 (MQ=255)                                                                                                                                                                                                  ctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTNAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCt                                                                                                   >  2:608064/1‑300 (MQ=255)                                                                                                                                                                                                  ctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCt                                                                                                   >  1:20267/1‑300 (MQ=255)                                                                                                                                                                                                  ctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCAACTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCt                                                                                                   >  1:450111/1‑300 (MQ=255)                                                                                                                                                                                                  ctTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCAACAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACATGGCAGGAGCAACATCATGACGCATGTTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCt                                                                                                   >  2:589896/1‑300 (MQ=255)                                                                                                                                                                                                  ctTATAGCTGCCACTCAATGAATGGCATCTTCCCCCATATCCACCCTGGCGAACTGTTTGTTTATCTTGGCATTACCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTACGCGGATGGGTGAACGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACCAACAGTTCACCTGGCAGGAGCAACATCATGGCGCATATTCGGATCGAAAAAGGAACAGGTGGGTGGGGCGGCCTGCt                                                                                                   >  2:63922/1‑300 (MQ=255)                                                                                                                                                                                                  ctTATAGCTGCCACTCAATGAACGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCt                                                                                                   >  2:538973/1‑300 (MQ=255)                                                                                                                                                                                                       aGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGc                                                                                              <  2:328758/300‑1 (MQ=255)                                                                                                                                                                                                              aCTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGc                                                                                       <  1:237711/300‑1 (MQ=255)                                                                                                                                                                                                               ctgcgagatgtgtataagagacagtatCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGcc                                                                                      >  1:675347/25‑300 (MQ=255)                                                                                                                                                                                                                 cAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCNCCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGACAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCAc                                                                                    >  2:167802/1‑300 (MQ=255)                                                                                                                                                                                                                 cAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCAc                                                                                    >  2:581853/1‑300 (MQ=255)                                                                                                                                                                                                                   atgaatgGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGc                                                                                  >  1:607369/1‑300 (MQ=255)                                                                                                                                                                                                                    tgaatgGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTATGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCAACAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGcc                                                                                 >  2:336999/1‑300 (MQ=255)                                                                                                                                                                                                                            ctctTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCANTTTCTTCCGCGGCTGGGTGACCGATCNGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCCGGCAGGAGCAACACCATGACGCATATTCGGATTGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCCTGAGCTGAAAGCCACACCGGaaaaaa                                                                         >  2:473102/1‑300 (MQ=255)                                                                                                                                                                                                                              ctTCCACCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTAGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGCCCGATCTGACCACCGCCATTTTTGNGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATCTTCGGATCGAAAAAGAAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCAACGCCGGGaaaaaaa                                                                       >  2:158642/1‑300 (MQ=255)                                                                                                                                                                                                                                 cccccATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGAAAAAAAAATc                                                                    >  1:195649/1‑300 (MQ=255)                                                                                                                                                                                                                                     cATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCAGGGAAAAAAAATCGTCt                                                                >  2:194124/1‑300 (MQ=255)                                                                                                                                                                                                                                     cATATCAACCCTGGCGAACTGTTTGTTTATCNTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCNCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCt                                                                >  2:495319/1‑300 (MQ=255)                                                                                                                                                                                                                                      atatCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCta                                                               >  2:384340/1‑300 (MQ=255)                                                                                                                                                                                                                                       tatCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCtat                                                              >  1:402559/1‑300 (MQ=255)                                                                                                                                                                                                                                            aCCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCAc                                                         >  1:181564/1‑300 (MQ=255)                                                                                                                                                                                                                                                       cTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAccc                                              <  2:246583/300‑1 (MQ=255)                                                                                                                                                                                                                                                       cTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGAACGATCTGACCACCGACATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTTGGATCGAAAAAGGACCGGGTGGTTGGGGCGGCCCGCTTGAGCTGAACGCCACGCCGGGAGAAAACATCGTCTATATCATCGCCGGTcccc                                              >  2:49140/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ttgtttATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGcc                                          <  1:101867/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           tGCCAGCGGTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaaa                          >  1:266859/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  ggTCTGACAACGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATATGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTNGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGCTCACCTGGCAGGAGCAACATCATGACNCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcac                   <  2:557087/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aCGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATCTCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActg          >  1:673577/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           aCGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActg          >  1:346571/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           aCGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCAACGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActg          >  1:221665/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            cGCTGATCCTGTCACTTTGCCCACTGGCGTTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCCGGAGCAACATCATGACGCATATTCGGNTCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTNCGATTGTTGACAAACTGGCACAGCTTActgg         <  2:479012/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            cGCTGAACCTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActgg         >  1:401125/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   ccTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACACACTGGCCCAGCTTACtggctggccg  >  1:547556/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                   ccTGTCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAg  >  1:547557/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        ATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCATTGCCAGCGGTCTGACAACGCTGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGACCGATCTGACCACCGCCATTTTTGAGAAAAAGATGGGCATTCAACTTGAACAAAAAGTTCACCTGGCAGGAGCAACATCATGACGCATATTCGGATCGAAAAAGGAACGGGTGGCTGGGGCGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAG  >  NC_000913/2825949‑2826538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑