Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,374,600	C→T	R135H (CGT→CAT)	menE ←	o‑succinylbenzoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,374,600	0	C	T	100.0%	181.5 / NA	54	R135H (CGT→CAT)	menE	o‑succinylbenzoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/23);  total (31/23)

CAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGA  >  NC_000913/2374311‑2374894                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       cAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTg                                                                                                                                                                                                                                                                                              <  2:449964/300‑1 (MQ=255)     cGCCAGAGTTGTGTTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCAc                                                                                                                                                                                                                                                                                          <  1:236791/300‑1 (MQ=255)          gagTTGTGTTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGc                                                                                                                                                                                                                                                                                     <  1:436233/300‑1 (MQ=255)            gTTGTGTTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGCTAAGTATGGACAGCGGCTCTCGGCAAACCGGTAGAAACCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCCCGTAGCgg                                                                                                                                                                                                                                                                                   >  2:84534/1‑300 (MQ=255)               gtgtTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCAt                                                                                                                                                                                                                                                                                >  1:574662/1‑300 (MQ=255)                   tGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTgc                                                                                                                                                                                                                                                                            >  1:96108/1‑300 (MQ=255)                      cACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGcc                                                                                                                                                                                                                                                                         <  1:263344/300‑1 (MQ=255)                                 cGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCt                                                                                                                                                                                                                                                              >  1:526932/1‑300 (MQ=255)                                    gAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGaa                                                                                                                                                                                                                                                           >  1:221202/1‑300 (MQ=255)                                     aGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAAt                                                                                                                                                                                                                                                          >  2:231291/1‑300 (MQ=255)                                       tACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAAtgt                                                                                                                                                                                                                                                        <  2:80933/300‑1 (MQ=255)                                           gCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGCACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGTAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATGAGTATGGAAAGCGGCTTTCGACAAACCGATAGAACCAGAGGTCAATATCATTGAGCACAGATGTGTTGGCTGCCACGTAGCGGCATGTGCACCTTCAACCAACTGAATGTGCAg                                                                                                                                                                                                                                                    >  2:25962/1‑300 (MQ=255)                                                ccAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACg                                                                                                                                                                                                                                               <  2:98992/300‑1 (MQ=255)                                                       ttGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGc                                                                                                                                                                                                                                        <  2:208004/300‑1 (MQ=255)                                                            ccactGGCTGTTTATCACGTACCGTCATCCGCGCCCCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTCGCAAGATGGGCATGATAAGTATGGACAGCGGCTTTCGGCAAACCAGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAAACAGCGGAATGTGCAGCGACGTTAATGCCAGaa                                                                                                                                                                                                                                   >  2:278285/5‑300 (MQ=255)                                                                     gTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGTGATAACACACCTTGCGCACTGGCAAGATGGGCATGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtttt                                                                                                                                                                                                                          >  2:286747/1‑300 (MQ=255)                                                                        tATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTccc                                                                                                                                                                                                                       <  1:548359/300‑1 (MQ=255)                                                                         aTCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTcccc                                                                                                                                                                                                                      >  1:82831/1‑300 (MQ=255)                                                                           cACGTACCGTCATCCGCGCACCAGCGTATAANCAGCGCCGCATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAAACAATCATCGTGATCGCCAAACGGAATCAGCGATAACACNCCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCCAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGCATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCAt                                                                                                                                                                                                                    >  2:499928/1‑300 (MQ=255)                                                                                          gcgcACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGGCGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACAGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGCCCGCGGCTTTCGGCAAACCGGTAGCACCCGAGGTCAACGTCGTTGAGCACCGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGTGACGTTAATGCCGGAAATGTGTTTTCCCCCCCCGGCACCAGAGCaa                                                                                                                                                                                                     >  1:678937/1‑300 (MQ=255)                                                                                                 aGCGTATAACCAGCGCCACATGATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAg                                                                                                                                                                                              <  2:199545/300‑1 (MQ=255)                                                                                                  gCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGANAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGNGACGTTAATGCCGGAAACGTGTTTTCCCCCTCCGGCACCAGAGCAAATTGCAGc                                                                                                                                                                                             >  2:638337/1‑300 (MQ=255)                                                                                                  gCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGc                                                                                                                                                                                             >  1:360546/1‑300 (MQ=255)                                                                                                   cGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCg                                                                                                                                                                                            >  1:399735/1‑300 (MQ=255)                                                                                                   cGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCg                                                                                                                                                                                            >  2:649500/1‑300 (MQ=255)                                                                                                                   caTAATTCCCTGACCGTAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCaa                                                                                                                                                                            <  1:232937/300‑1 (MQ=255)                                                                                                                              gACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCa                                                                                                                                                                 >  1:577487/1‑300 (MQ=255)                                                                                                                                     gaCGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGNTAGAACCCGAGGTCAACGTCATTGAGNACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTg                                                                                                                                                          >  2:455939/1‑300 (MQ=255)                                                                                                                                      aCGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGc                                                                                                                                                         >  1:458798/1‑300 (MQ=255)                                                                                                                                      aCGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGc                                                                                                                                                         >  2:383730/1‑300 (MQ=255)                                                                                                                                                  ggTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAggg                                                                                                                                             >  1:75708/1‑300 (MQ=255)                                                                                                                                                          gagCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACggg                                                                                                                                     <  2:394258/300‑1 (MQ=255)                                                                                                                                                          gagCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACggg                                                                                                                                     >  1:522569/1‑300 (MQ=255)                                                                                                                                                                 cAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACAcc                                                                                                                              <  1:149726/300‑1 (MQ=255)                                                                                                                                                                 cAATCANCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCANGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCTGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACAcc                                                                                                                              <  2:570735/300‑1 (MQ=255)                                                                                                                                                                                              cGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACgccagcca                                                                                                 <  1:326863/300‑1 (MQ=255)                                                                                                                                                                                                                   ggCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCACGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGTCAGGCGAGCAGCGTTTGCGGCGt                                                                            >  2:426814/1‑300 (MQ=255)                                                                                                                                                                                                                             gCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGcc                                                                  <  1:211350/300‑1 (MQ=255)                                                                                                                                                                                                                              ccTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGccc                                                                 >  2:299558/1‑300 (MQ=255)                                                                                                                                                                                                                                     aGTATGGACAGCGGCTTTCGGCAAACTGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACAACCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACAt                                                          >  1:579427/1‑300 (MQ=255)                                                                                                                                                                                                                                     aGTATGGACAGCGGCTTTCGGCAAACCGGTCGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCACATCCGGCACCAGAGCCAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGTGTTTGCGGCGTATTCCACGCCCGCAACAt                                                          >  2:139474/1‑300 (MQ=255)                                                                                                                                                                                                                                     aGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACAt                                                          >  1:328429/1‑300 (MQ=255)                                                                                                                                                                                                                                                       cGGCAAACCGGTAGAACCCGAGTTCAACGTCATTGAGCACAGATGCGTCGGCTGNCACGTAGCGGCATGTGCGCCTTCAACCCGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAac                                        <  2:521035/300‑1 (MQ=255)                                                                                                                                                                                                                                                       cGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAac                                        <  1:240162/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAATCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACTACCCCCTGCAc                            <  1:595727/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCAc                            <  2:144719/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aGAACCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCAc                            <  1:86145/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      aCCCGAGGTCAACGTCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGc                         >  1:359196/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gTCAACGGCAGTGAGCACAGATGCGTCGGCTGCAACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCTGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTACAGCGTAAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGGGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACACCAGCCATGCGAGCAGCGTTTGCGGCGTATTCCACGCCTGCAACATCACGCCGCGGCCCTCAacctgtctcttatacacatctcgc                  <  1:230448/300‑22 (MQ=255)                                                                                                                                                                                                                                                                                    tCATTGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAggata           >  1:375336/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                        tGAGCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTc       <  1:285160/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATc    >  1:547677/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gCACAGATGCGTCGGCTGCCACGTAGCGGCATGTGCGACTTCAAACAGCTGAATGTGCAGCGGCGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCATTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCATGCACCGCAAATCCGGAGGCTAATTCATc    >  2:63893/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             acaGATGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGTAGGCTAATTCATCGa  <  2:144084/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       CAAACGCCAGAGTTGTGTTGGCACCAGTGAAGCGTGAGTACAGCCTGCCAGCATTTGCTCCAATGGCTGTTTATCACGTACCGTCATCCGCGCACCAGCGTATAACCAGCGCCACATAATTCCCTGACCGGAGACGTGAAACAGCGGTAAAGAGAGCAACCAATCATCGTGATCGCCAAACGGAATCAGCGATAACACACCTTGCGCACTGGCAAGATGGGCCTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGCAGCAATTCTTCAAGCAACGGTTGCGGCAGCTGAGGGTTCACGGGCAACACCCGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGA  >  NC_000913/2374311‑2374894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑