Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,373,773	C→T	V411M (GTG→ATG)	menE ←	o‑succinylbenzoate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,373,773	0	C	T	100.0%	301.3 / NA	86	V411M (GTG→ATG)	menE	o‑succinylbenzoate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/51);  total (35/51)

GCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCC  >  NC_000913/2373478‑2374068                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         gCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGCTTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGACATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCAt                                                                                                                                                                                                                                                                                                      >  2:133751/1‑300 (MQ=255) gCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCAt                                                                                                                                                                                                                                                                                                      >  1:262429/1‑300 (MQ=255)     aaGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCAc                                                                                                                                                                                                                                                                                                  >  1:226146/1‑300 (MQ=255)       gTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTa                                                                                                                                                                                                                                                                                                >  1:569786/1‑300 (MQ=255)               cTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAAc                                                                                                                                                                                                                                                                                        >  1:3244/1‑300 (MQ=255)                    aTCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGctct                                                                                                                                                                                                                                                                                   >  2:122204/1‑300 (MQ=255)                      cttttttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATTAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTNTAGCGCCTGACTTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATNTTTCATCCATTCACTAAGATCAACGCTCTCg                                                                                                                                                                                                                                                                                 <  2:499389/296‑1 (MQ=255)                           ttATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTc                                                                                                                                                                                                                                                                            <  2:391464/300‑1 (MQ=255)                               tGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATAc                                                                                                                                                                                                                                                                        <  2:104598/300‑1 (MQ=255)                                 cagcaCGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTc                                                                                                                                                                                                                                                                      <  2:242407/300‑1 (MQ=255)                                 cagcaCGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTc                                                                                                                                                                                                                                                                      <  1:262945/300‑1 (MQ=255)                                 cagcaCGATTTTTTGACCAGCCACTCCATTGCATTATCCTGTTTGCTAAGGGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGNAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTc                                                                                                                                                                                                                                                                      <  1:473712/300‑1 (MQ=255)                                       gATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCAc                                                                                                                                                                                                                                                                <  1:295029/300‑1 (MQ=255)                                        aTTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCaac                                                                                                                                                                                                                                                               >  1:419074/1‑298 (MQ=255)                                         ttttttGACCAGCCACTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCg                                                                                                                                                                                                                                                              <  2:357805/300‑1 (MQ=255)                                             ttGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCAc                                                                                                                                                                                                                                                          <  2:25349/300‑1 (MQ=255)                                                   aGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCg                                                                                                                                                                                                                                                    >  1:434751/1‑300 (MQ=255)                                                       attccattGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCCTCACCGCCACCGGTCGATGa                                                                                                                                                                                                                                                >  2:364370/1‑300 (MQ=255)                                                               gCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTc                                                                                                                                                                                                                                        >  1:28980/1‑300 (MQ=255)                                                               gCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTc                                                                                                                                                                                                                                        >  2:437172/1‑300 (MQ=255)                                                               gCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGACCAACGCTCTCGTGGTCATACTCCATCAACGCCAACGGTCGATGAACAAAccc                                                                                                                                                                                                                                        >  2:357351/1‑298 (MQ=255)                                                                       cTGTTTGCGAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCgg                                                                                                                                                                                                                                <  2:34958/300‑1 (MQ=255)                                                                           ttGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAAc                                                                                                                                                                                                                           >  1:347569/1‑300 (MQ=255)                                                                             gCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGACATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTAGG‑CAACgg                                                                                                                                                                                                                         >  1:98939/1‑300 (MQ=255)                                                                                aaGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGa                                                                                                                                                                                                                      <  1:395843/300‑1 (MQ=255)                                                                                    gTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGANTGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCAGCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGAt                                                                                                                                                                                                                  <  2:517244/300‑1 (MQ=255)                                                                                                               gATAAACTCTTTATCAACGTCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACCCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTgc                                                                                                                                                                                       <  2:413467/300‑1 (MQ=255)                                                                                                                cttagcaaacaggataatgCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACTTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCCCCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAAACGCAGGATGTgca                                                                                                                                                                                      >  2:309658/19‑300 (MQ=255)                                                                                                                    aCTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCaa                                                                                                                                                                                  <  1:402322/300‑1 (MQ=255)                                                                                                                     ctctTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTNTTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGCGTTAGCCAGCGCACCGGTTGTTGAAACCGGGCCAGCTCATCTTTAATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTNCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAAt                                                                                                                                                                                 >  2:643055/1‑300 (MQ=255)                                                                                                                     ctctTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTNCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGNAGCAAt                                                                                                                                                                                 >  2:561831/1‑300 (MQ=255)                                                                                                                       ctTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTa                                                                                                                                                                               <  1:87343/300‑1 (MQ=255)                                                                                                                         ttagcgaCGGCAATGGAATGGTAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTTACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTAcg                                                                                                                                                                             <  2:232767/294‑1 (MQ=255)                                                                                                                         ttATCAACGGCAATGGAATGGCAGAAATTAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTAcg                                                                                                                                                                             <  2:623402/300‑1 (MQ=255)                                                                                                                                       ggaatggCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTAAGCGCTCGACttcttc                                                                                                                                                               >  2:527817/1‑300 (MQ=255)                                                                                                                                                    tGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAccc                                                                                                                                                  <  1:86728/300‑1 (MQ=255)                                                                                                                                                            agagacaggggTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGNGAAATTTTAATACCGCCGTTTTTCAGCCCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGcc                                                                                                                                          <  2:520796/292‑1 (MQ=255)                                                                                                                                                                  gAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAACCTCTCCGCCACTgaa                                                                                                                                    >  1:71824/1‑300 (MQ=255)                                                                                                                                                                   aGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTgaag                                                                                                                                   <  1:48503/300‑1 (MQ=255)                                                                                                                                                                    gggTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGccctgtctct                                                                                                                                  >  1:531134/1‑292 (MQ=255)                                                                                                                                                                      gTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAAt                                                                                                                                <  1:146259/300‑1 (MQ=255)                                                                                                                                                                        tAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAg                                                                                                                              <  2:210414/300‑1 (MQ=255)                                                                                                                                                                         ggttattGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACAGGTTGTTGAAAACGGGCCAGCTTATCTTTCATTCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCGCCCGGTCGATGACCACGCTCATTGTCGGTCAA‑GGATACTATAAACACCTACAAAACCGCAAGCTGTGAAGCACCTACGCGCTCGACTTTTTCCGGCTGAATACTCTCTACGTCACTGAAGAATAGa                                                                                                                             >  2:285504/3‑300 (MQ=255)                                                                                                                                                                         aaTTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTAACATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGATTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCCTCCCCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACAACTGCAGAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCCAAATACCCTCTACGCCACTGAAGAATAGa                                                                                                                             >  2:285491/1‑300 (MQ=255)                                                                                                                                                                                    aCGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACg                                                                                                                  <  1:140892/300‑1 (MQ=255)                                                                                                                                                                                    aCGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACg                                                                                                                  <  2:234829/300‑1 (MQ=255)                                                                                                                                                                                    aCGTTACACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACg                                                                                                                  <  2:665329/300‑1 (MQ=255)                                                                                                                                                                                               aCTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATgg                                                                                                       <  1:331735/300‑1 (MQ=255)                                                                                                                                                                                                ctctTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGt                                                                                                      >  1:9354/1‑300 (MQ=255)                                                                                                                                                                                                  ctTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCAACGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGAATTCTTCCGGCTGAATACCCTCTACGCCACTGAAGAATAGATTGTCTAAACGTCCGACCATGGTcg                                                                                                    >  2:336957/1‑299 (MQ=255)                                                                                                                                                                                                    tttAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGc                                                                                                  <  1:260655/300‑1 (MQ=255)                                                                                                                                                                                                           ccTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCAt                                                                                           <  1:339664/300‑1 (MQ=255)                                                                                                                                                                                                                cGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGc                                                                                      <  1:67144/300‑1 (MQ=255)                                                                                                                                                                                                                  tGAAATTTTAAGACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTTAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAt                                                                                    <  2:675784/300‑1 (MQ=255)                                                                                                                                                                                                                     aagtttAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGGGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtctc                                                                                 <  2:89164/297‑1 (MQ=255)                                                                                                                                                                                                                     aaTTTTAATACCGCCGTTTTTCAGCTCCGTCGGCATAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACGAAAATCAACGCTCGCGTGGTCATACTCCATCACCGCCACCGTTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCGCGACTTCTTCCTGCTGAATACCCTCTCCGCCACCGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtctc                                                                                 <  2:88575/300‑1 (MQ=255)                                                                                                                                                                                                                     aaTTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTTAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtctc                                                                                 <  1:692606/300‑1 (MQ=255)                                                                                                                                                                                                                     aaTTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtctc                                                                                 <  1:298010/300‑1 (MQ=255)                                                                                                                                                                                                                             tACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTTAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGAt                                                                         <  1:86596/300‑1 (MQ=255)                                                                                                                                                                                                                                    gTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgt                                                                  >  1:406186/1‑300 (MQ=255)                                                                                                                                                                                                                                     tttttCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgta                                                                 >  1:684375/1‑300 (MQ=255)                                                                                                                                                                                                                                        ttCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCNCCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtagcg                                                              <  2:596547/300‑1 (MQ=255)                                                                                                                                                                                                                                            gCTCCGNCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATNTTTCATCCATTCACTAAGATCAACGCTCTCGTGTTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATCAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTTAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTCTGCATCTCACCGCGATCGCGCGTAGCGTAcc                                                          <  2:575853/300‑1 (MQ=255)                                                                                                                                                                                                                                               ccGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCAATGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGc                                                       >  1:621429/1‑300 (MQ=255)                                                                                                                                                                                                                                                cggcggCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTNATCCATTCACTAAGATCAACGCTCTCGCGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                      <  2:509553/300‑1 (MQ=255)                                                                                                                                                                                                                                                cggcggCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                      <  1:587126/300‑1 (MQ=255)                                                                                                                                                                                                                                                cggcggCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                      <  1:145252/300‑1 (MQ=255)                                                                                                                                                                                                                                                cggcggCAGAGGGAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCCACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGcc                                                      <  2:433538/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gagTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAt                                              >  1:283516/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gagTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCAGGCTGAATACCCTCTACGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCACGTATGCATCTCACCGCGATCGCGCGTAGCGTACAAGCCTTCGTCAt                                              >  2:34051/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gagTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGAACAAACTCATTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCCTTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCAt                                              >  2:223186/1‑300 (MQ=255)                                                                                                                                                                                                                                                             aGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAAcc                                         <  1:264174/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAAcc                                         <  1:112650/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      aCCGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAAcc                                <  2:111604/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       ccGGTTGTTGAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGGCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCANAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCACGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCa                               <  2:168285/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gttgAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGNATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGcc                         >  2:596843/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gttgAAAACGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTACGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGCTCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAGCCAGTTGcc                         >  2:127969/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    aCGGGCCAGCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACg                  <  2:89917/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gCTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGtcac          >  2:139005/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                             cTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGTTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAAcc         <  2:281289/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             cTTATCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAAcc         <  1:287903/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aTCTTTCATCCATTCACTAAGATCAACACTCTCGTGGTCATACTCCATCACCGCCACCGTTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTGTGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTc      <  2:210115/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                aTCTTTCATCCAGTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGTGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCACGATCACGCTTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTGGCCCGTTACGCCAGTAACCTTc      <  2:210360/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 tCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCt     >  2:408377/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 tCTTTCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCACGTTACGCCAGTAACCTTCt     >  2:394017/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    ttCATCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAANCCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTCAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGcc  <  2:604252/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         GCATAAGTACATGTCTGTTATCTTGTTTATTGCAGCACGATTTTTTGACCAGCCATTCCATTGCATTATCCTGTTTGCTAAGAGTTTTCACATCAATTTACAGATTAACAGATAAACTCTTTATCAACGGCAATGGAATGGCAGAAATGAGAAGTGAAACGGAGGGTTAATTATTGCTGACGTTGCACCCACTCTTTTAGCGCCTGACGTGAAATTTTAATACCGCCGTTTTTCAGCTCCGGCGGCAGAGTTAGCCAGCGCACCGGTTGTTGAAAACGGGCCAGCTTATCTTTCACCCATTCACTAAGATCAACGCTCTCGTGGTCATACTCCATCACCGCCACCGGTCGATGACCAAACTCCTTGTCGG‑CAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAGCGTACCAGCCTTCGTCATTAACCAGTGAAACCAGTTGCCCGTTACGCCAGTAACCTTCTGCC  >  NC_000913/2373478‑2374068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑