Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,328,771	C→T	A343T (GCG→ACG)	yfaQ ←	tandem DUF2300 domain‑containing protein YfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,328,771	0	C	T	100.0%	170.9 / NA	50	A343T (GCG→ACG)	yfaQ	tandem DUF2300 domain‑containing protein YfaQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/21);  total (29/21)

TCGGGATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGC  >  NC_000913/2328482‑2329057                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                               tCGGGATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGTGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAggg                                                                                                                                                                                                                                                                                      <  1:74136/300‑1 (MQ=255) tCGGGATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAggg                                                                                                                                                                                                                                                                                      <  2:112926/300‑1 (MQ=255)    ggATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACNCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGc                                                                                                                                                                                                                                                                                   >  1:493435/1‑300 (MQ=255)    ggATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGc                                                                                                                                                                                                                                                                                   >  1:63263/1‑300 (MQ=255)                    gTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTcgc                                                                                                                                                                                                                                                                   >  2:376272/1‑300 (MQ=255)                            tCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGa                                                                                                                                                                                                                                                           >  1:320994/1‑300 (MQ=255)                              gTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAAc                                                                                                                                                                                                                                                         <  2:268279/300‑1 (MQ=255)                              gTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAAc                                                                                                                                                                                                                                                         <  1:373083/300‑1 (MQ=255)                              gTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCNGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAAc                                                                                                                                                                                                                                                         <  1:463066/300‑1 (MQ=255)                                  cGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCATTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTg                                                                                                                                                                                                                                                     >  2:35835/1‑300 (MQ=255)                                      ctcCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCaa                                                                                                                                                                                                                                                 >  1:348280/1‑300 (MQ=255)                                          cTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGNAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCCTAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCNCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCAAGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTa                                                                                                                                                                                                                                             >  2:482762/1‑300 (MQ=255)                                                cGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTc                                                                                                                                                                                                                                       >  1:671370/1‑300 (MQ=255)                                                cGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTAAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTc                                                                                                                                                                                                                                       >  2:7003/1‑300 (MQ=255)                                                                                 cccTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGcc                                                                                                                                                                                                      >  1:154301/1‑300 (MQ=255)                                                                                                         aTAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTAACGATCGAGATGCGACTGCAATTTCCGTTTGCCCTTTTCGCTCAGTAAGGCCGtctc                                                                                                                                                                              >  2:530735/1‑300 (MQ=255)                                                                                                                            gCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTa                                                                                                                                                           >  1:438355/1‑300 (MQ=255)                                                                                                                                aGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtgt                                                                                                                                                       <  2:10803/300‑1 (MQ=255)                                                                                                                                aGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtgt                                                                                                                                                       <  1:35920/300‑1 (MQ=255)                                                                                                                                 gATGAGATCCTGCGTCCAGGCGGTCATCGCTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTAGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGATTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAAtgtg                                                                                                                                                      >  1:296281/1‑300 (MQ=255)                                                                                                                                     agaTCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATNCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTa                                                                                                                                                  >  1:492521/1‑300 (MQ=255)                                                                                                                                                       gTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTcaca                                                                                                                                >  2:534520/1‑300 (MQ=255)                                                                                                                                                       gTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCAAGATGCGAATGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTAACATTAGTAAAGGTcaca                                                                                                                                >  2:242577/1‑300 (MQ=255)                                                                                                                                                          aTCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTNTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGa                                                                                                                             >  1:488021/1‑300 (MQ=255)                                                                                                                                                           tCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGAt                                                                                                                            <  1:348906/300‑1 (MQ=255)                                                                                                                                                                cgcgCCCCCGTGGTCGCTGTCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGTGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAgcgc                                                                                                                       <  1:84943/300‑1 (MQ=255)                                                                                                                                                                cgcgCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGGAAAAAAGTGCGAANCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAgcgc                                                                                                                       <  1:505242/300‑1 (MQ=255)                                                                                                                                                                        cGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAg                                                                                                               <  2:628161/300‑1 (MQ=255)                                                                                                                                                                          tGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCATGCAATCGCCTTCCCGATTGGCGTTTTGGTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCATGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTa                                                                                                             <  2:61785/300‑1 (MQ=255)                                                                                                                                                                                        gCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACt                                                                                               >  1:543545/1‑300 (MQ=255)                                                                                                                                                                                           gAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGcc                                                                                            >  1:433561/1‑300 (MQ=255)                                                                                                                                                                                           gAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCAAACGATAGCGCCCATTAAGTACGCCGGGATTTACTGcc                                                                                            >  1:639254/1‑300 (MQ=255)                                                                                                                                                                                                   cTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCAGGGATTTACTGCCGTCGTAct                                                                                    >  1:388102/1‑300 (MQ=255)                                                                                                                                                                                                    ttcGTGGCGCTGCTGTCGTGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGTGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCTAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTActc                                                                                   <  2:187022/298‑1 (MQ=255)                                                                                                                                                                                                    ggCGTGGCGCTGCTGTCGGGGATGGTCATACAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCGACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTActc                                                                                   <  2:197886/299‑1 (MQ=255)                                                                                                                                                                                                       gTGGCACTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCAGTGCTTGCGTTGCCTCAGTGGGCNTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATGTCAGTTTGCCCTTTTCGCTCAGTAAGGGCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACGGCCGTCGTACGCttt                                                                                <  2:603777/300‑1 (MQ=255)                                                                                                                                                                                                          gcgcTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGTGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTcc                                                                             >  1:654908/1‑300 (MQ=255)                                                                                                                                                                                                          gcgcTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCAAACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTcc                                                                             >  1:11712/1‑300 (MQ=255)                                                                                                                                                                                                               gctgTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGGTGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTAACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCtgt                                                                        >  2:364944/1‑300 (MQ=255)                                                                                                                                                                                                               gctgTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTAAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTAACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTAACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAATGTCAAACGATAGCGCCCATTAAGTACGCAGGGATTTACTGCCGTCGTACTCTTTTCCGCtgt                                                                        >  2:123471/1‑300 (MQ=255)                                                                                                                                                                                                                  gTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGAt                                                                     >  1:75479/1‑300 (MQ=255)                                                                                                                                                                                                                                ggCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGAt                                                       >  1:450751/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gCGTTTTGCTTTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGAGCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGTTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCAcc                                   <  1:553203/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCACTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCAcc                                   >  1:81036/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gCTGTAAAAAAGTGCGAATTGCCACGGTCATTGCTTTCGTTGCCTCAGTGTGTGTACTTTTCGCCTCGCGATCCAGAACTCTTTCAACGTACTCTTCACGATCTAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCCTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTacac                            <  2:202208/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGTGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACAATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACTTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTacac                            <  2:234069/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTacac                            <  1:210353/300‑1 (MQ=255)                                                                                                                                                                                                                                                             tGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACAt                          <  1:317264/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            aTCGCCACGGTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCGCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAAcc           <  1:130703/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gTCATGGCTTTCGTTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTATGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTAAGCGGATAGCGGGCAAACAGCTCCAACTCTACACATTGCCCACTGGCAACCTGCGTCGGc  >  2:619902/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                               TCGGGATAAGCAAACGCGAGTATCTGATCGTAACGCTCTCCCTGCCCCGCCTGGGCCGTGGCTTGCCGCCAGGAAAGTGTCCCTTCGGTAGCGCGACTGCCATGATAATGAACCGGATCGCCTGCATAGATGAGATCCTGCGTCCAGGCGGTCATCGTTCGCGCCCCCGTGGTCGCTGGCGAAGCAGAAACACGCTGCGTGGCGCTGCTGTCGGGGATGGTCAGGCAATCGCCTTCCCGATTGGCGTTTTGCTGTAAAAAAGTGCGAATCGCCACGGTCATGGCTTTCGCTGCCTCAGGGGGCGTACTTTTCGCCTCGCGATCCAGAACTCTGGCAACGTACTCTTCACGATCGAGATGCGACTGCAATTTCAGTTTGCCCTTTTCGCTCAGTAAGGTCGTCTCACCGTGGCTGACAAACGTAATGTGGTTACCATTAGTAAACGTCACACGATAGCGCCCATTAAGTACGCCGGGATTTACTGCCGTCGTACTCTTTTCCGCTGTGATTTTCTTCAGCGGATAGCGGGCAAACAGCTCCACCTCTACACATTGCCCACTGGCAACCTGCGTCGGC  >  NC_000913/2328482‑2329057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑