Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,608,785	C→T	V80I (GTT→ATT)	yneE ←	PF01062 family inner membrane protein YneE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,608,785	0	C	T	100.0%	98.6 / NA	34	V80I (GTT→ATT)	yneE	PF01062 family inner membrane protein YneE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/18);  total (16/18)

TCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGT  >  NC_000913/1608490‑1609061                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                      tCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAAAAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGAGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATGCGGCAATGTCGTTTTTACCTAGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTaa                                                                                                                                                                                                                                                                                   >  2:588198/1‑300 (MQ=255)           gTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGCGATAATGAGCCAGCACTTCCGCNTGTGGCTGTTTGCGTAATGTCATGCGNAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAGCTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAGCCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTAcc                                                                                                                                                                                                                                                                         >  2:452868/1‑300 (MQ=255)                         tGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTGAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGa                                                                                                                                                                                                                                                           <  2:343693/300‑1 (MQ=255)                               gCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGCCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCa                                                                                                                                                                                                                                                     >  1:127184/1‑300 (MQ=255)                                       ttCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAat                                                                                                                                                                                                                                             >  1:621743/1‑300 (MQ=255)                                                             cGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGNCCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATg                                                                                                                                                                                                                      >  1:508710/1‑300 (MQ=255)                                                                   gCTGGAGAGGTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTTAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAAc                                                                                                                                                                                                                <  1:367385/300‑1 (MQ=255)                                                                   gCTGGAGAATTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGACACTATTACGAAAACCAGGGGGAATGGTGATGTGGA‑CACCGAGAATGCTGAAc                                                                                                                                                                                                                <  2:340136/300‑1 (MQ=255)                                                                           gTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAg                                                                                                                                                                                                        <  1:349218/300‑1 (MQ=255)                                                                                           cGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGccc                                                                                                                                                                                        >  1:597496/1‑300 (MQ=255)                                                                                                   aTCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCgt                                                                                                                                                                                <  1:590994/300‑1 (MQ=255)                                                                                                        cAGTCTTTAGATAGTGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATGTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTANCTCGCGCAGTAACGACCGTTAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCATACCCTGCATTATTACGAAAACCAATAGGAATGGTAAGGGCAA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTAcc                                                                                                                                                                           <  2:172126/300‑1 (MQ=255)                                                                                                          gTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCNTGAGGCAATCATCAACTGCCCCCAAANTTTTCGCGCTTCAATGTAACGGGCGTACCCTGCATTATTACGAAAACCAATAAAAATGACAATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAg                                                                                                                                                                         <  2:475887/300‑1 (MQ=255)                                                                                                             tttagaTAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCTGCAATGTCGTTTTTACCTCGCGCAGTAACGACCTTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACTGGCGTACCCTGCATTATTACGAAAACCAATGGTAATGGCGATG‑CGAACACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgc                                                                                                                                                                      <  2:116291/297‑1 (MQ=255)                                                                                                             ttGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgc                                                                                                                                                                      <  1:444106/300‑1 (MQ=255)                                                                                                             gtGAGATAATGAGCCAGTACTTCCTCCGTTGGCTGTTTGCGTAATGTCATTCGTAAACATTGGGCGAAAGCGATTTGCAGTCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTTATGCGATCATCACCTGCTCCCAAAGTTTCCGCGCTTCAATTTAACGTGCGTACCCTTCACTACTACTCAACCCAGGGGCAATGATACTGTTGC‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGgc                                                                                                                                                                      <  2:181971/299‑1 (MQ=255)                                                                                                                                                  tGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGa                                                                                                                                 >  1:300971/1‑300 (MQ=255)                                                                                                                                                   gCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCTTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGag                                                                                                                                >  1:408991/1‑300 (MQ=255)                                                                                                                                                   gCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGag                                                                                                                                >  1:44791/1‑300 (MQ=255)                                                                                                                                                          gTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAg                                                                                                                         >  1:67320/1‑300 (MQ=255)                                                                                                                                                              tGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGcgcg                                                                                                                     >  1:546121/1‑300 (MQ=255)                                                                                                                                                                                 atctaTTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAAGGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGNTTCAATGTAACGGGCGTACCCTGCATTATTACGAAAACCAAGAAAAATGGCGATGTCGA‑CANCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATAc                                                                                                  <  2:546086/296‑1 (MQ=255)                                                                                                                                                                                                        cccTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATAc                                                                           >  1:101534/1‑300 (MQ=255)                                                                                                                                                                                                        cccTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCACAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTCCGAAAACCAAGAAAAATGGCGATGGCGA‑AGCCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGAAGCATGAAAATAACCGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGCGGATCTCTTGGatgatgctgcgtcgtgtcgggcaagactat                                                                           >  2:271966/1‑272 (MQ=255)                                                                                                                                                                                                                      gAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAgtgt                                                             >  1:406844/1‑300 (MQ=255)                                                                                                                                                                                                                      gAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCAAgtgt                                                             >  1:407524/1‑300 (MQ=255)                                                                                                                                                                                                                                          tACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCAt                                         <  1:668966/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gcAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCt                                 <  1:600977/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCa                              >  1:268596/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aaCGACCGTTAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAATTTAACGGGCGTACCCGGCATTATTACGAAAACCAATAAAAATGGCGATGCCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAgg                            <  2:104572/300‑1 (MQ=255)                                                                                                                                                                                                                                                         cGACCGTGAGGCAATCATCAACTGCCCCCAAAGTCTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAACAATTGCGATGTCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCNTGTACCAGGGCAGCATGAAAATAACANCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCg                          <  2:471049/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gCCCCCAAATTTTTCGCGCTTCAATGTAACGGGCGTACCCGGCATTATTACGAANACCAAGAAAAATGGCAATGTCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTNTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACg   <  2:636855/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gCCCCCAAAGTTTTCGCGCTTCAATGTAACGTGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGTCGA‑CACCGAGAATGCTGAACGGTGCGAGGGNGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACg   <  2:608759/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cccccAAAGTTTTCGCACTTCAATGTAACTTGCGTACCCGGCACTACTACGAAAACCAAGAAAAATGGCGATGTCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGt  <  2:99148/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                      TCTGAAAGCTGTCCATTGCGGCGCTGAACCGCCAACCATTCTCCCATTATTAACAAGATACGGTTAGCTGGAGAGTTCGAAGCCAGTACACGCTGAAGATCTTCAGTCTTGAGATAATGAGCCAGCACTTCCGCCTGTGGCTGTTTGCGTAATGTCATGCGTAAACAGTGGGCGAAAGCGATTTGCAGCCGGGCAAACTCCCTTACACTTGCCGAATCCGGCAATGTCGTTTTTACCTCGCGCAGTAACGACCGTGAGGCAATCATCAACTGCCCCCAAAGTTTTCGCGCTTCAACGTAACGGGCGTACCCGGCATTATTACGAAAACCAAGAAAAATGGCGATGGCGA‑CACCGAGAATGCTGAACGGTGCGAGGGTGAATTTGATGCCCAGATGCGTGTACCAGGGCAGCATGAAAATAACAGCGATAGAAAAGAGAAAATTGAGTAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGT  >  NC_000913/1608490‑1609061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑