Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	554,191	G→A	L83L (CTG→TTG)	ppiB ←	peptidyl‑prolyl cis‑trans isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	554,191	0	G	A	100.0%	204.6 / NA	60	L83L (CTG→TTG)	ppiB	peptidyl‑prolyl cis‑trans isomerase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/21);  total (39/21)

GAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGT  >  NC_000913/553908‑554483                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                     gagaTGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCAATTGCCAACGTACCACGGGTAttt                                                                                                                                                                                                                                                                                      >  2:334206/1‑300 (MQ=255)    aTGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTc                                                                                                                                                                                                                                                                                   >  1:205897/1‑300 (MQ=255)              gCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCgttgt                                                                                                                                                                                                                                                                         >  1:631754/1‑300 (MQ=255)                                      aCTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCtt                                                                                                                                                                                                                                                 >  2:403947/1‑300 (MQ=255)                                       cTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCANATACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACNACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCttt                                                                                                                                                                                                                                                >  2:541732/1‑300 (MQ=255)                                       cTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCANACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACNACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCttt                                                                                                                                                                                                                                                >  2:541684/1‑300 (MQ=255)                                         cGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTtgg                                                                                                                                                                                                                                              <  1:567316/300‑1 (MQ=255)                                          gCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGcctgtctcttatacacatctcgcag                                                                                                                                                                                                                                             <  1:423318/300‑25 (MQ=255)                                                    aCGCTTTCAATGATAACATCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGt                                                                                                                                                                                                                                   <  2:116092/300‑1 (MQ=255)                                                         ttCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCAt                                                                                                                                                                                                                              >  1:246835/1‑300 (MQ=255)                                                           cAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCAGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGc                                                                                                                                                                                                                            >  1:216051/1‑300 (MQ=255)                                                                       cttcttTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAAc                                                                                                                                                                                                                <  1:354879/300‑1 (MQ=255)                                                                                    gTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAAt                                                                                                                                                                                                   >  1:17627/1‑300 (MQ=255)                                                                                    gTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTAGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAAt                                                                                                                                                                                                   >  1:549205/1‑300 (MQ=255)                                                                                    gTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTAAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAAt                                                                                                                                                                                                   >  1:336435/1‑300 (MQ=255)                                                                                    gTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGGCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAAt                                                                                                                                                                                                   <  1:95634/300‑1 (MQ=255)                                                                                     tCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCNATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTAGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATNGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATc                                                                                                                                                                                                  >  2:637239/1‑300 (MQ=255)                                                                                     tCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCNATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCAAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATNGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATc                                                                                                                                                                                                  >  2:637420/1‑300 (MQ=255)                                                                                     tCCTGGTGCATACCGCTACGACCGGTTGCAACAACTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATc                                                                                                                                                                                                  >  2:239236/1‑300 (MQ=255)                                                                                     tCCTAGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGCCTACCAAGTCCATGCCGTCAACCCCTTAAGCAAACACGCAGTAGCCCCCACCTTGCAGGCTTTCGACAGAGAAGTTCAGGAAGTCGTTATCAACCAAGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTCCCAAGGGTATTTTTCAGGCCGGTGTTGGCTTAGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATc                                                                                                                                                                                                  >  1:571931/1‑300 (MQ=255)                                                                                         ggTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTCCCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTAAGGACGTTGTTGGCTTCGTTTTTGATCGGTTTTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATcctga                                                                                                                                                                                              >  2:45659/1‑296 (MQ=255)                                                                                         ggTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGNCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTNCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATaa                                                                                                                                                                                              >  2:648293/1‑300 (MQ=255)                                                                                            gCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTGCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGNTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGNGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGc                                                                                                                                                                                           <  2:545302/300‑1 (MQ=255)                                                                                            gCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCATGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGc                                                                                                                                                                                           <  2:177587/300‑1 (MQ=255)                                                                                            gCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGc                                                                                                                                                                                           <  1:387897/300‑1 (MQ=255)                                                                                                      aCGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAac                                                                                                                                                                                 <  1:307653/300‑1 (MQ=255)                                                                                                                                tGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTaaa                                                                                                                                                       >  2:450547/1‑300 (MQ=255)                                                                                                                                                 ccGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCagtag                                                                                                                                      >  1:677919/1‑300 (MQ=255)                                                                                                                                                         cACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCATAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGaa                                                                                                                              <  2:61054/300‑1 (MQ=255)                                                                                                                                                          aCTTCANCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCNGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAg                                                                                                                             <  2:569091/300‑1 (MQ=255)                                                                                                                                                           cTTCAGCACACACGCAGTAGCCCCAACCTTGCAGGCTTTCGACAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCAATTGCCAACGTACCAAGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCCACACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGACAATGGTGTTGTTGTACACACCTTCGCGTCAGTAGTCCAGGAAGt                                                                                                                            >  2:255950/1‑300 (MQ=255)                                                                                                                                                                             agcaccAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTg                                                                                                          >  2:198147/5‑300 (MQ=255)                                                                                                                                                                             aGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTg                                                                                                          >  1:148693/1‑300 (MQ=255)                                                                                                                                                                             aGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTg                                                                                                          >  2:198078/1‑300 (MQ=255)                                                                                                                                                                                    aCCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATc                                                                                                   >  1:593835/1‑300 (MQ=255)                                                                                                                                                                                                              tCAGTAAGTCGTTATCAACCACTTGGAGGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCATGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTTGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGc                                                                         <  2:112726/300‑1 (MQ=255)                                                                                                                                                                                                              tCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCTTGAGTACTTGCCATTGCCAACGTACCACGGGTATTTTTCATGCCGTTGTTTGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGc                                                                         <  2:185592/300‑1 (MQ=255)                                                                                                                                                                                                              tCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGc                                                                         <  1:578434/300‑1 (MQ=255)                                                                                                                                                                                                                     gTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCATGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGAtt                                                                  <  1:14963/300‑1 (MQ=255)                                                                                                                                                                                                                     gTCGGTATCAACCATGTTGATGGAGGACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGTCGTTGTTGGTTTCGTTTGTGATCGGGTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAAGAATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGAtt                                                                  <  1:34988/300‑1 (MQ=255)                                                                                                                                                                                                                              agccACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTTAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTCTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGaa                                                         <  2:523914/298‑1 (MQ=255)                                                                                                                                                                                                                              aaacACGTTGATGAAGAACTGTTCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCGTTGCCAACGTACCACGGGTATTGTGCAGGCCGTTGTTTGCTTCGTTTGTAATTGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTGGTGGTGAAAACCTTCGCGGCATTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGaa                                                         <  2:359026/297‑1 (MQ=255)                                                                                                                                                                                                                                ccACGTTGATGAAGAACTGTGCGGTTGCAGAGTGCGGAGCCTGGGTACGTGCCATTGCCAACATACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCTTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAg                                                       <  2:25016/300‑1 (MQ=255)                                                                                                                                                                                                                                        atgcagaaCTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCAtt                                               >  1:347999/5‑300 (MQ=255)                                                                                                                                                                                                                                                cTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATc                                       >  1:602909/1‑300 (MQ=255)                                                                                                                                                                                                                                                cTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATc                                       >  2:656079/1‑300 (MQ=255)                                                                                                                                                                                                                                                 tgtgCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATcc                                      <  1:573353/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gtgCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAANTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCt                                     >  2:516227/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGAGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTcc                                >  2:118843/1‑300 (MQ=255)                                                                                                                                                                                                                                                         tGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGt                              <  1:188841/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGAtt                        >  2:408820/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTGCGGAGCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGAtt                        >  1:251460/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGANGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCtt                 >  1:493377/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCtt                 >  2:530115/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gCCTGAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCtt                 >  1:247668/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATCTCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAAc             >  1:25497/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gAGTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTNCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAAc             >  2:157281/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATTGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCAGTTTGATTGGTGCTTCAAccc           >  2:345208/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            gTACGTGCCATTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAAccc           >  1:140474/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     aTTGCCAACGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAANACGGTGGAAAATGGTGTTGTTGTAAANACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGt  >  2:472636/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                     GAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCTTGCAGGCTTTCGCCAGAGAAGTTCAGGAAGTCGTTATCAACCACGTTGATGAAGAACTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATTGGTGTGGAAAGTAACCATTTTTGCATCCTGTTCCGTTTGATTGGTGCTTCAACCCAGTTCGGGT  >  NC_000913/553908‑554483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑