Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,351,196 (G)7→8 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,1961.G96.3% 197.5 / 1.4 54intergenic (‑157/+211)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (18/34);  total (19/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑G‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGT  >  NC_000913/1350911‑1351483
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                               
tttGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTNATGCTTGTTGTGCCTGAAAATCAGGCNATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTc                                                                                                                                                                                                                                                                                    <  2:470930/300‑1 (MQ=255)
         cTGGTAGGTGAATGCCGGTTCAGCTCCTTCGCGGTGCATTGCCTGAGCGATACCGTAGGCGATGGATAGTTTTCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCTACCTGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAAcc                                                                                                                                                                                                                                                                           <  2:374492/300‑1 (MQ=255)
          tGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAAccc                                                                                                                                                                                                                                                                          <  1:11804/300‑1 (MQ=255)
          tGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAAccc                                                                                                                                                                                                                                                                          <  2:308181/300‑1 (MQ=255)
          tGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGNAGGCGATGGATAGTTTGCTTGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCNGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAAccc                                                                                                                                                                                                                                                                          <  2:643193/300‑1 (MQ=255)
           ggTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCTACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCct                                                                                                                                                                                                                                                                         >  1:399666/1‑300 (MQ=255)
               ggTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTcc                                                                                                                                                                                                                                                                     >  1:367667/1‑300 (MQ=255)
                    aTGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGATCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                                                                                                                                <  2:434833/300‑1 (MQ=255)
                    aTGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                                                                                                                                <  1:557827/300‑1 (MQ=255)
                      gCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                                                                                                                              <  2:5782/300‑1 (MQ=255)
                      gCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                                                                                                                              <  1:455891/300‑1 (MQ=255)
                          gTTCAGCTCCTTCGCGGTGCATCGCCTGAGCNATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGANGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCg                                                                                                                                                                                                                                                          >  2:486860/1‑300 (MQ=255)
                                    ttCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                                                                                                                                                                                                <  1:75512/300‑1 (MQ=255)
                                        cgGTGCATCGCCTGAGCGATACCTTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTNATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACNCTCTCCCCAGAGGGGCGAGGGGACCGTATtg                                                                                                                                                                                                                                            <  2:501558/300‑1 (MQ=255)
                                          gTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                                                                                                                                          >  1:149413/1‑300 (MQ=255)
                                           tGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGc                                                                                                                                                                                                                                         <  1:24034/300‑1 (MQ=255)
                                                cGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAata                                                                                                                                                                                                                                    >  1:170041/1‑300 (MQ=255)
                                                          aTACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcccc                                                                                                                                                                                                                          >  1:661495/1‑300 (MQ=255)
                                                            aCCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTTTGCAAATATTGTTACCCCAg                                                                                                                                                                                                                        >  1:309679/1‑300 (MQ=255)
                                                                    cGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaac                                                                                                                                                                                                                <  1:377845/300‑1 (MQ=255)
                                                                        ggATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc                                                                                                                                                                                                            <  1:306150/300‑1 (MQ=255)
                                                                                   tGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc                                                                                                                                                                                                 <  1:297128/300‑1 (MQ=255)
                                                                                             ggTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCtttt                                                                                                                                                                                       >  1:404386/1‑300 (MQ=255)
                                                                                             ggTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTACCAAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCACCTAACCCttt                                                                                                                                                                                       >  2:267206/1‑300 (MQ=255)
                                                                                                tgccAGAGTGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCCTATTGTTGACGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCTGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCAt                                                                                                                                                                                    <  2:49598/298‑1 (MQ=255)
                                                                                                     gAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCAACCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAACTTTTCATGGCGa                                                                                                                                                                               >  2:416397/1‑300 (MQ=255)
                                                                                                               aCCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAGCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGAc                                                                                                                                                                     <  2:417840/300‑1 (MQ=255)
                                                                                                               aCCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTNTTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGAc                                                                                                                                                                     <  2:611441/300‑1 (MQ=255)
                                                                                                               aCCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCTGAGCAGGCTGCGGCAGGGGG‑GTG‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGAc                                                                                                                                                                     <  1:147678/300‑1 (MQ=255)
                                                                                                               aCCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGAc                                                                                                                                                                     <  1:395294/300‑1 (MQ=255)
                                                                                                                    aaagaaaACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGtgc                                                                                                                                                                <  1:154327/300‑3 (MQ=255)
                                                                                                                        aaaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTATTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCATAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGACGGCTTGGACGGTTCAGAc                                                                                                                                                            <  2:88916/300‑1 (MQ=255)
                                                                                                                         aaaCCCATCGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCTTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                                                                                                                           <  2:204444/300‑1 (MQ=255)
                                                                                                                         aaaCCCATATCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCGGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                                                                                                                           <  1:536235/300‑1 (MQ=255)
                                                                                                                         aaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCATGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTTGGACGGTTCAGAcc                                                                                                                                                           <  1:105143/300‑1 (MQ=255)
                                                                                                                         aaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCATAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                                                                                                                           <  1:536250/300‑1 (MQ=255)
                                                                                                                         aaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                                                                                                                           <  1:534951/300‑1 (MQ=255)
                                                                                                                         aaaCCCATAGCTTTAATCCTTATTGTTGATGCTTGGTGTGCCTGAAAATCAGTCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCCCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGACGGCTGGGACGGTTCAGAcc                                                                                                                                                           <  1:318375/300‑1 (MQ=255)
                                                                                                                              cATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                                                                                                                                      <  1:12257/300‑1 (MQ=255)
                                                                                                                                  gCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                                                                                                                                  >  2:670749/1‑300 (MQ=255)
                                                                                                                                            ttATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTAGAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                                                                                                                                        <  1:15301/300‑1 (MQ=255)
                                                                                                                                              aTTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGTTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAc                                                                                                                                      <  2:202167/300‑1 (MQ=255)
                                                                                                                                              aTTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTCTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAc                                                                                                                                      <  2:676946/300‑1 (MQ=255)
                                                                                                                                                      tGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGCTGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtctaca                                                                                                                              >  2:368667/1‑297 (MQ=255)
                                                                                                                                                                                       gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCt                                                                                             <  1:429630/300‑1 (MQ=255)
                                                                                                                                                                                                             gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTCACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCCCCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGgag                                                                       >  1:647434/1‑300 (MQ=255)
                                                                                                                                                                                                             gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGgag                                                                       >  1:413943/1‑300 (MQ=255)
                                                                                                                                                                                                                                      ggCTGTGAGTAGCTATAGTTGCCAGGTCCGAACGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCACAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCACCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATCCTCCAGCACTGTCTCCATGTTTTCCCCCCAACAGCTACTCCGCTCGGTCTGACCCCTCGCTCTTTCAGGGAGAGGGTGATCGGTGGTTCCGtttctt                                              >  2:141614/1‑296 (MQ=255)
                                                                                                                                                                                                                                            gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAat                                        <  1:236545/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cGGAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAATCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGGGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATTGCGATGGCTTGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAc              <  2:72377/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gAGCAGGCTGCGGCAGGGGGAG‑G‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAGCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTTACCAAAACAGCTATTTTGCTCGGTCTGCCCCCCCGCTCTTTCAGGGAGAGGGTGAAAGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTaaaa            >  2:283236/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                        gAGCAGGCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCCGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCCGCACCGTCTCCTCATTCcaa            >  1:584658/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                             ggCTGCGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTAcaatcaat       >  1:326738/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                  cGGCAGGGGG‑G‑GGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCTTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTACATGTTTTCACCAAAACAGCTATTCGGCTCGGTCTACCCCCTCGCCCTTTCAAGGAGAGGGTGCAAGGCGGTTCAGTTCCTGCAGAATATTCTCAAGCACCGTCTCATCATTACAATCAATCTCGt  >  2:182610/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                               
TTTGTCGTTCTGGTAGGTGAATGCCAGTTCAGCTCCTTCGCGGTGCATCGCCTGAGCGATACCGTAGGCGATGGATAGTTTGCTGGCAACACCGGTTACCAGAATGCGCTTACCGGAAAGAAAACCCATAGCTTTAATCCTTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGG‑G‑G‑CGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGCTATTCGGCTCGGTCTGCCCCCTCGCTCTTTCAGGGAGAGGGTGACCGGCGGTTCAGTTCCTGCAGAATATTCTCCAGCACCGTCTCCTCATTACAATCAATCTCGT  >  NC_000913/1350911‑1351483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑