Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	288,641	G→A	A174A (GCC→GCT)	xynR ←	DNA‑binding transcriptional repressor XynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	288,641	0	G	A	100.0%	195.3 / NA	55	A174A (GCC→GCT)	xynR	DNA‑binding transcriptional repressor XynR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/26);  total (29/26)

GCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCC  >  NC_000913/288347‑288934                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      gcggcgACGGCTGCTAAGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCAGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGCAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTAGTGGTTCCACACCGGCACCGACACGCAGCGCACGCCCTGCTCGTTGTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTATGCGCCAGGGCGATCACTAAAGCTTc                                                                                                                                                                                                                                                                                                  >  2:210413/1‑300 (MQ=255)                       cTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTggcgggc                                                                                                                                                                                                                                                                            >  1:278153/1‑300 (MQ=255)                                 ttCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAg                                                                                                                                                                                                                                                                  <  1:584199/300‑1 (MQ=255)                                      cTACGAGCCAGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTAc                                                                                                                                                                                                                                                             <  2:137653/300‑1 (MQ=255)                                         cGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGa                                                                                                                                                                                                                                                          <  2:239789/300‑1 (MQ=255)                                                         cTCAGGCCGGTTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtac                                                                                                                                                                                                                                          <  2:469137‑M1/300‑6 (MQ=255)                                                         cTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtac                                                                                                                                                                                                                                          <  1:745579‑M1/300‑6 (MQ=255)                                                         cTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCAGTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtac                                                                                                                                                                                                                                          <  1:589983‑M1/300‑6 (MQ=255)                                                              gCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTNCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGNAGTGTACTGATAGCCCTCCAGtgtactgacc                                                                                                                                                                                                                                     <  2:525597‑M1/300‑11 (MQ=255)                                                                 gggTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgacccca                                                                                                                                                                                                                                  >  1:30804‑M1/1‑287 (MQ=255)                                                                     aGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaa                                                                                                                                                                                                                              >  1:431886‑M1/1‑283 (MQ=255)                                                                           gcgcgcgcGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTNCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCNTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAGAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttgga                                                                                                                                                                                                                        >  2:597448‑M1/1‑277 (MQ=255)                                                                                      agCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacac                                                                                                                                                                                                             <  1:237728‑M1/300‑35 (MQ=255)                                                                                      agCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTAGGGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacac                                                                                                                                                                                                             <  1:78367‑M1/300‑35 (MQ=255)                                                                                                  gCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatatagg                                                                                                                                                                                                 <  1:22817‑M1/300‑47 (MQ=255)                                                                                                    ctgcttAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGTAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCTAGACGCGAGGGTGGCGGGCGTAAAGTTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtc                                                                                                                                                                                               <  2:193097‑M1/300‑49 (MQ=255)                                                                                                      gctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctg                                                                                                                                                                                             >  1:130512‑M1/1‑250 (MQ=255)                                                                                                       ctgAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctga                                                                                                                                                                                            >  1:360671‑M1/1‑249 (MQ=255)                                                                                                                       aaaTTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaatgg                                                                                                                                                                            <  1:274068‑M1/300‑68 (MQ=255)                                                                                                                                aGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTNCGCCAGGGCGCTCATTAAAGCTTCGCNAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagac                                                                                                                                                                   <  2:497906‑M1/300‑77 (MQ=255)                                                                                                                                aGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagac                                                                                                                                                                   <  1:108028‑M1/300‑77 (MQ=255)                                                                                                                                 gCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagacc                                                                                                                                                                  >  1:323181‑M1/1‑223 (MQ=255)                                                                                                                                                    ggAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGCTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTAGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCAAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatgttcaattggagccagaccttttaatttccggctactt                                                                                                                                               >  2:34824‑M1/1‑204 (MQ=255)                                                                                                                                                                      gCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtat                                                                                                                             >  1:57678‑M1/1‑186 (MQ=255)                                                                                                                                                                           gggCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaat                                                                                                                        >  1:405606‑M1/1‑181 (MQ=255)                                                                                                                                                                                                 gTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttc                                                                                                  <  1:128520‑M1/300‑142 (MQ=255)                                                                                                                                                                                                    gTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCATTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgacccgaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcagnctacttcttctgctgttgtagtattcaatatattccgtaacagcatccttaagt                                                                                               >  2:547153‑M1/1‑156 (MQ=255)                                                                                                                                                                                                                  ccGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattaat                                                                                 >  1:380921‑M1/1‑142 (MQ=255)                                                                                                                                                                                                                    gCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactga                                                                               >  1:483788‑M1/1‑140 (MQ=255)                                                                                                                                                                                                                      cACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCNTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaac                                                                             <  1:513625‑M1/300‑163 (MQ=255)                                                                                                                                                                                                                      cACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaac                                                                             <  1:738334‑M1/300‑163 (MQ=255)                                                                                                                                                                                                                       aCGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaact                                                                            >  2:569720‑M1/1‑137 (MQ=255)                                                                                                                                                                                                                            gcgcACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatca                                                                       >  1:138579‑M1/1‑132 (MQ=255)                                                                                                                                                                                                                                 cGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagata                                                                  >  1:666113‑M1/1‑127 (MQ=255)                                                                                                                                                                                                                                      ttctcGTTCTCTTCGCTGTCCAGTGCTTAGCCTTGCTCGCGGGTCTGCGCCATGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTAATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattgtagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccttaacagcatccttcagttcgcttatattactgaactcatcaagataaaaac                                                             <  2:216523‑M1/298‑179 (MQ=255)                                                                                                                                                                                                                                       gCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaaca                                                            >  1:490611‑M1/1‑121 (MQ=255)                                                                                                                                                                                                                                                     aagagacaggcgtagcctTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaagg                                              <  1:49013‑M1/292‑194 (MQ=255)                                                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAACGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatctttcagttcgcttgtattaatgaactcatcaagataaaaacaccccgaactcaaggtt                                            >  2:82343‑M1/1‑105 (MQ=255)                                                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggtt                                            >  1:614558‑M1/1‑105 (MQ=255)                                                                                                                                                                                                                                                       gTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggtt                                            >  2:447016‑M1/1‑105 (MQ=255)                                                                                                                                                                                                                                                            gggCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggntaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaa                                       >  1:517925‑M1/1‑100 (MQ=255)                                                                                                                                                                                                                                                              gCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgacctcatcaagataaaaacactccgactttaaggttccaaaaa                                     >  2:30144‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                                                              gCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaaga                                     >  2:30354‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                                                              gCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggctgtctctt                                     >  2:48898‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                                                                         cTCGCGGGTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatgcttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccacc                          <  1:723314‑M1/300‑214 (MQ=255)                                                                                                                                                                                                                                                                               ggTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagca                    >  2:219378‑M1/1‑81 (MQ=255)                                                                                                                                                                                                                                                                               ggTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagca                    >  1:694440‑M1/1‑81 (MQ=255)                                                                                                                                                                                                                                                                                gTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttanacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcat                   <  1:538435‑M1/300‑221 (MQ=255)                                                                                                                                                                                                                                                                                gTCTGCGCCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtacggaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcat                   <  1:677183‑M1/300‑221 (MQ=255)                                                                                                                                                                                                                                                                                    gcgcCAGGGCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcactatattccgtaacagcatccttccgttcgcttctattactgaactcatcaatataaagacactccgaatttaaagttcaaaagaaatacgccaccacagcattatc               >  2:431301‑M1/1‑76 (MQ=255)                                                                                                                                                                                                                                                                                          gggCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGGGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcctatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcattatccagaca         <  2:633426‑M1/300‑231 (MQ=255)                                                                                                                                                                                                                                                                                          gggCGCTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTNCTGATAGCCCTCCAGtgtactgaccccgaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcattatccagaca         <  2:540447‑M1/300‑231 (MQ=255)                                                                                                                                                                                                                                                                                            gcgcTCATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcattatccagacaat       <  2:437546‑M1/300‑233 (MQ=255)                                                                                                                                                                                                                                                                                                 cATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacactaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcattatccagacaattgcct  <  1:717537‑M1/300‑238 (MQ=255)                                                                                                                                                                                                                                                                                                 cATTAAAGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGtgtactgaccccaaaaagttggacagttaaacacgaggcatataggtctgattccgatattcaattggagtcagaccttttaatttcaggctaattcttctgctgttgtagtattcaatatattccgtaacagcatccttcagttcgcttatattactgaactcatcaagataaaaacactccgactttaaggttccaaagaaacactccaccacagcattatccagacaattgcct  >  1:471718‑M1/1‑63 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      GCGGCGACGGCTGCTACGCGGACTTCGACTACTTCACCTACGAGCCGGTCTAACGGCTCAGGCCGGGTAGCCCAGCGCGCGCGAGAGCGCGAGCCCGGCCTGCTGAAGCTGCTCGCGGAAATTAGCCAGCTCCGCGTCGTCCACGCGGGAGGTCAGCGTCGACAGGCTCAGGGCGGCGATGACGCGGGACTCGTGGTTCCACACCGGCACCGCCACGCAGCGCACGCCCTGCTCGTTCTCTTCGCTGTCCAGGGCGTAGCCTTGCTCGCGGGTCTGCGCCAGGGCGCTCATTAAGGCTTCGCGAGACGCGAGGGTGGCGGGCGTAAAGGTAGTGTACTGATAGCCCTCCAGCAGGGCGTTCAGCTCGGCCTCGCCCAGCCAGGCAATCAACACCTTGCCGATGGCGGTGGCGTGCACCGGCAGGCGGCGGCCGATGCGTGAATAGGCGATGGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCC  >  NC_000913/288347‑288934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑