Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,348	G→A	G187D (GGC→GAC)	yagH →	putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,348	0	G	A	100.0%	148.0 / NA	41	G187D (GGC→GAC)	yagH	putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/23);  total (18/23)

ATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTA  >  NC_000913/287065‑287639                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    ggtGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGCGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAAAACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTAGCCCGAGCGCAAAACGATGTTTACCGACACGCCGCTCTGATACACCAAAGACGCGAACCTGTAtaga                                                                                                                                                                                                                                                                                     >  2:423186/3‑297 (MQ=255)                tGGAAAAACGGCCGCAACTTCCTCGTCACCACGCCCTCCATCGAGGGTCCAGGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGANGGCCGCAAATACTATACCTACCGCCCGTGGGGTCCGCGCCACCACAGCAACCCGCACAACANCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGTTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGtac                                                                                                                                                                                                                                                                      <  2:585301/300‑1 (MQ=255)                    aaaaCGGCCGCAACTTCCTCGTCACCCCACCCCCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTGGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGNCACCACAGCAACCCGCACAACACCATNGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCTCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTAcc                                                                                                                                                                                                                                                                  <  2:512317/300‑1 (MQ=255)                     aagcGGCCGCAACTCCCTCTTCACTGCGCCCTCCATCGAGGGGCCATTGAGCGAGCCAATCCCGATGGGAAACGGCGGGTTTGACCCGCCCCTGTTCCACGACGACGATGGCCGAAAATACTATATCGACCGCCCGTGGGGGCCGCGCCACCACAACAACCCGCACAACACCATCGTGTGACAGGCGTTTGACCCACAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTGACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                                                                                 <  2:298232/297‑1 (MQ=255)                     aaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCGTCGAGGGGCCATGGATCGAGCCAACCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCACAAATACTATACCTACCGCCCGTGGGGGCCGCGCCACCACAACAACCCGCACAACACCATCGTGTTACAGGCGTTGGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCt                                                                                                                                                                                                                                                                 <  2:255066/300‑1 (MQ=255)                     aaaCGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGGGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCt                                                                                                                                                                                                                                                                 <  2:201304/300‑1 (MQ=255)                                  ttCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTCTATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTTTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAg                                                                                                                                                                                                                                                    <  1:119698/300‑1 (MQ=255)                                         tCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCa                                                                                                                                                                                                                                             >  1:297732/1‑300 (MQ=255)                                          cACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCAc                                                                                                                                                                                                                                            <  1:60716/300‑1 (MQ=255)                                                   cTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGACACCACAGCAACTCGCACAACACCATCGTGTTACAGGCGTCTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCCAAACGCTGTTTACCGACACGCCGCTCTGCTACAACGAAGACACGCACCTGTATCGCAACGCGGGATGGTACTACCTGacggaaaaatagggtgacacaagctacga                                                                                                                                                                                                                                   >  2:63674/1‑272 (MQ=255)                                                    tCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAg                                                                                                                                                                                                                                  <  1:604887/300‑1 (MQ=255)                                                             gggCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCcgtc                                                                                                                                                                                                                         >  1:625304/1‑300 (MQ=255)                                                                         gagcCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGAAAAGACTATATCTACCGCCTGTGGGGGCCGTACCACCAAAGCAACCCACACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGAAAAACATTGTTGACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGt                                                                                                                                                                                                             <  1:551321/300‑1 (MQ=255)                                                                          agcCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATNGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACNTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGtt                                                                                                                                                                                                            >  2:678777/1‑300 (MQ=255)                                                                          agcCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCATGTTCCACGACGACGATNGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTGTGACCCGCAGCCCGGCACGCTCTCGCCCGAGCGCAAAACGATGTTTACCGACACGCCGCTCTGCTACACCGAAGAGGCGTACCTGTATCGCCAAGCGGGATGGTACTAANTGATGGACGCAGAGGGCGGCCCCAGCTAAGCGCACGCCGTCGTGGTGCTGCGtt                                                                                                                                                                                                            >  2:679695/1‑300 (MQ=255)                                                                                                             tCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCAAGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAAGGGCCGTACGAGCTGCACCCg                                                                                                                                                                         >  1:33105/1‑300 (MQ=255)                                                                                                                                         aaTACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCAcc                                                                                                                                             <  1:313008/300‑1 (MQ=255)                                                                                                                                          aTACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCt                                                                                                                                            >  1:289711/1‑300 (MQ=255)                                                                                                                                          aTACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGAAGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCt                                                                                                                                            >  1:222234/1‑300 (MQ=255)                                                                                                                                           tACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTg                                                                                                                                           >  1:346816/1‑300 (MQ=255)                                                                                                                                                                                          caacaCCATCGGGTTACAGGCGTTTGACCCGAAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTGACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGAc                                                                                            <  2:387778/300‑1 (MQ=255)                                                                                                                                                                                                   tgtgtTAGAGGTGTTGGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACggg                                                                                   <  2:210032/299‑1 (MQ=255)                                                                                                                                                                                                      gtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGa                                                                                >  1:715551/1‑300 (MQ=255)                                                                                                                                                                                                             gCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTAc                                                                         <  2:572925/300‑1 (MQ=255)                                                                                                                                                                                                              cGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACa                                                                        >  1:624884/1‑300 (MQ=255)                                                                                                                                                                                                               gTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACAt                                                                       <  2:621169/300‑1 (MQ=255)                                                                                                                                                                                                                  tGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACGCGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGTGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                                                    <  1:198175/300‑1 (MQ=255)                                                                                                                                                                                                                  tGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTANTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGc                                                                    <  2:650514/300‑1 (MQ=255)                                                                                                                                                                                                                   gACCCGCGGACCGGCACGCTCTCGCCCGAGCGCAAACCGCTGTTTACCGACACGCCGCTCTGCTACACCTAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGTGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGcc                                                                   <  2:116988/300‑1 (MQ=255)                                                                                                                                                                                                                       cGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTAcc                                                               <  1:472773/300‑1 (MQ=255)                                                                                                                                                                                                                         cAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTc                                                             <  1:187815/300‑1 (MQ=255)                                                                                                                                                                                                                            aCCGGCACACTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGNACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCAcc                                                          <  2:164477/300‑1 (MQ=255)                                                                                                                                                                                                                             ccGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCNGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCa                                                         <  1:506496/300‑1 (MQ=255)                                                                                                                                                                                                                                    gCTCTCGCCCGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGccgccc                                                  >  1:218537/1‑300 (MQ=255)                                                                                                                                                                                                                                            ccGAGCGCAAAACGCTGTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCATAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGcc                                          <  2:674386/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aaaCGCTGTTTACCGACACGCCGCTTTGCTACACCGAAGACGCGCACCTGTATCGCCACGCTGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCg                                 <  2:395917/300‑1 (MQ=255)                                                                                                                                                                                                                                                          cTGTTTAACGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGATGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCTGGCGTGCCg                            >  2:392266/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gTTTACCGACACGCCGCTCTGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCgct                          >  1:313063/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ctGCTACACCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGGTGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGNCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGAcg        >  2:642241/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    cacCGAAGACGCGCACCTGTATCGCCACGCGNGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGNTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGTTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCGGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCGGGCCTCCGACGGACGCGGCTa  >  2:515957/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    cacCGAAGACGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGNCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTa  >  2:640288/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    ATTGTCGACTCGCCGTGGAAAAACGGCCGCAACTTCCTCGTCACCGCGCCCTCCATCGAGGGGCCATGGAGCGAGCCAATCCCGATGGGCAACGGCGGGTTTGACCCGTCCCTGTTCCACGACGACGATGGCCGCAAATACTATATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTGCTACACCGAAGGCGCGCACCTGTATCGCCACGCGGGATGGTACTACCTGATGGCCGCCGAGGGCGGCACCAGCTACGAGCACGCCGTCGTGGTGCTGCGTTCCAAAAATATCGACGGGCCGTACGAGCTGCACCCGGACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTA  >  NC_000913/287065‑287639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑