Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,701,306 A→G H117R (CAC→CGC)  malY → negative regulator of MalT activity/cystathionine beta‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,701,3060AG97.7% 151.6 / NA 43H117R (CAC→CGC) malYnegative regulator of MalT activity/cystathionine beta‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (16/26);  total (16/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATAT  >  NC_000913/1701010‑1701600
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
ggATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCcgcac                                                                                                                                                                                                                                                                                                     <  1:120381/300‑1 (MQ=255)
ggATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGNTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGANCcgcac                                                                                                                                                                                                                                                                                                     >  2:612049/1‑300 (MQ=255)
  aTTATGTCGCTGACCGTTTCGGCACTGCTTACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATGACACCGCCATCGATTTGCAGACGGTGGTATATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCgcacac                                                                                                                                                                                                                                                                                                   <  1:367845/300‑1 (MQ=255)
                  tttCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCAtt                                                                                                                                                                                                                                                                                   >  1:259173/1‑300 (MQ=255)
                          ctgctgACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAgg                                                                                                                                                                                                                                                                           >  1:620932/1‑300 (MQ=255)
                                           ttCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAg                                                                                                                                                                                                                                                          <  1:717268/300‑1 (MQ=255)
                                                gATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGTCGTATTTGGCTACAGCCGCTTGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCNACCCAGCATTACACCGCCATCGAGTCGCAGACGGTGGTGTATGGCCCTTCGGTCATCTATATGGTTTCAGAACTGACTCGCCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGATGCATTTTACAAGGCCATTGAAGGTAACCAGCGcac                                                                                                                                                                                                                                                     <  2:174832/300‑1 (MQ=255)
                                                   ttCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGNCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGt                                                                                                                                                                                                                                                  <  1:511978/300‑1 (MQ=255)
                                                   ttCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGt                                                                                                                                                                                                                                                  <  1:596454/300‑1 (MQ=255)
                                                   ttCAGACATGGATTTTGCCACTGCCAAATGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGt                                                                                                                                                                                                                                                  <  1:79144/300‑1 (MQ=255)
                                                                      aCTGCCCCCTGCATTATCTAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTNGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACNCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTagag                                                                                                                                                                                                                               <  2:683050/300‑1 (MQ=255)
                                                                      aCTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTGGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTagag                                                                                                                                                                                                                               <  1:682777/300‑1 (MQ=255)
                                                                              cTGCATTATCTAGGCGCTGAATCAGCGCCTGATGCACGGCGTATGTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTGCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGc                                                                                                                                                                                                                       <  1:213421/300‑1 (MQ=255)
                                                                              cTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGc                                                                                                                                                                                                                       >  2:415387/1‑300 (MQ=255)
                                                                                                gAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGa                                                                                                                                                                                                     <  1:592193/300‑1 (MQ=255)
                                                                                                                  cGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCTGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATNGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGc                                                                                                                                                                                   <  1:537685/300‑1 (MQ=255)
                                                                                                                            ggCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCg                                                                                                                                                                         <  1:291045/300‑1 (MQ=255)
                                                                                                                                                          ctcGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTgtgt                                                                                                                                           <  1:473271/300‑1 (MQ=255)
                                                                                                                                                            cgcgGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAg                                                                                                                                         <  1:676443/300‑1 (MQ=255)
                                                                                                                                                                gCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCca                                                                                                                                     >  1:77570/1‑300 (MQ=255)
                                                                                                                                                                gCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTAAAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACAAGAATGTAAAATTATGCTCCTGTGTAGCCca                                                                                                                                     >  1:592789/1‑300 (MQ=255)
                                                                                                                                                                 cTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCcac                                                                                                                                    >  1:584485/1‑300 (MQ=255)
                                                                                                                                                                                          gCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCTGGAAAGTGTGGAc                                                                                                           <  1:97948/300‑1 (MQ=255)
                                                                                                                                                                                          gCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGAc                                                                                                           >  1:268018/1‑300 (MQ=255)
                                                                                                                                                                                          gCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGAc                                                                                                           >  2:116900/1‑300 (MQ=255)
                                                                                                                                                                                           cATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACg                                                                                                          <  1:40409/300‑1 (MQ=255)
                                                                                                                                                                                                                  gACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGc                                                                                   <  1:742670/300‑1 (MQ=255)
                                                                                                                                                                                                                      gtggtgTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCANGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTAACGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTgcc                                                                               >  1:540470/1‑298 (MQ=255)
                                                                                                                                                                                                                      gtggtgCATGGCCCTCCTGTCGTCTCTATGGNTTCCGCACTGCTTCGTCAGTGGTCTGAAACAGGTGAAGGTGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCAATTGAAGGTANCCAGCGCACAGTCATGACCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGCTATGGGCCAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCATGTGTAGCACACAGAATCCTCAAGGGAAAGTGTGGACGTGTGATGAGCTGGAGATTATGGCTgcc                                                                               >  2:529072/1‑298 (MQ=255)
                                                                                                                                                                                                                       tggtgTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGAcc                                                                              <  1:274780/300‑1 (MQ=255)
                                                                                                                                                                                                                       tggtgTATGGCCCTTCTGTCATCTATATGGTTGCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGAcc                                                                              <  1:274781/300‑1 (MQ=255)
                                                                                                                                                                                                                         gtgtATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACANAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCtg                                                                            >  2:554869/1‑300 (MQ=255)
                                                                                                                                                                                                                         gtgtATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCtg                                                                            >  2:694920/1‑300 (MQ=255)
                                                                                                                                                                                                                         gtgtATGGCCCCTCTGTCATCTATATGGTTTCAGAACTAATTCGTCAGTGGTCTGAAACAGGTGAAGACGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCtg                                                                            <  2:32087/300‑1 (MQ=255)
                                                                                                                                                                                                                           gtATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTAACGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCtgtg                                                                          >  1:152826/1‑300 (MQ=255)
                                                                                                                                                                                                                                gCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGc                                                                     >  1:424181/1‑300 (MQ=255)
                                                                                                                                                                                                                                 cccTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAgtg                                                                    >  2:294045/1‑298 (MQ=255)
                                                                                                                                                                                                                                       tttCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCTGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATgg                                                              <  1:213310/298‑1 (MQ=255)
                                                                                                                                                                                                                                          cATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGgtgt                                                           >  2:96317/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                         tGGTCTGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAATGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCatatggat                            <  1:71840/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                              tGAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCTGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGt                       <  1:456076/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gAAACAGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGtt                      <  2:29116/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    aGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTggtg                 <  1:614983/300‑3 (MQ=255)
                                                                                                                                                                                                                                                                                    aGGTGAAGGCGTGGTGATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTgggg                 <  1:437164/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   gATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACATTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCatat  <  1:264739/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   gATCCGCACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCatat  <  1:12715/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
GGATTATGTCGCTGACCGTTTCGGCACTGCTGACCTGTTACCGTTCACGATTTCAGACATGGATTTTGCCACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATAT  >  NC_000913/1701010‑1701600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑