Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	97.1%	111.3 / NA	34	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (17/16);  minor base A (0/1);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGG  >  NC_000913/284254‑284813                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                       gACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGCTCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGAGCCGGAAGGTTCGGTGATc                                                                                                                                                                                                                                                                      >  2:220261/1‑300 (MQ=255) gACTATCAACCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGTATCTGGACGCCATGACCGTGACCGGCCGGACGGTGGGCGAGAGTCTTGAATGGTGGCAGGCGTCCGAGTGACGGGCGCGTTTACGCCAGTGCCTGCGCGAGCAGGAAGGCGTAGAGCCGGATGACGTGACCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTAACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAGGGTTCGGTGATc                                                                                                                                                                                                                                                                      >  2:114366/1‑300 (MQ=255)   cTATCACCCGAACGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGAGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGANCTCGAAGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCaa                                                                                                                                                                                                                                                                    >  2:594574/1‑300 (MQ=255)      tCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGc                                                                                                                                                                                                                                                                 >  1:359025/1‑300 (MQ=255)      tCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGAAGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGc                                                                                                                                                                                                                                                                 >  1:359153/1‑300 (MQ=255)                gTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGNTTCCCGACGGGCAACATCGCGACGGAAGGTTCGGTGATCAAGGCCACNGCGATc                                                                                                                                                                                                                                                       >  2:660591/1‑300 (MQ=255)                          tCCTCGCGGGCGGCGTGCCGTAGGTGATGCTCCACCTGCTCGACTTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCTTCgg                                                                                                                                                                                                                                             <  1:451363/300‑1 (MQ=255)                          tCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTAACCGGCCAGACGGTGTGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCgg                                                                                                                                                                                                                                             <  2:12243/300‑1 (MQ=255)                                                ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTAGGTGGTGGGCGAAGATGGCGtata                                                                                                                                                                                                                       >  2:49813/1‑300 (MQ=255)                                                    aTGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGTGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGGCGGTGGTGGGCGAAGATGGCGTATaccac                                                                                                                                                                                                                   <  1:111924/300‑1 (MQ=255)                                                         ccACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCacac                                                                                                                                                                                                              <  1:155387/300‑1 (MQ=255)                                                                       tCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcggg                                                                                                                                                                                                <  1:354991/300‑1 (MQ=255)                                                                        cGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgggt                                                                                                                                                                                               <  1:99838/300‑1 (MQ=255)                                                                                 gcATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGNGGTGGGCGAAGATGGCGTATACCACCAAACCGGCCGGGTGCGGGTGTNTGtcta                                                                                                                                                                                      >  2:654262/1‑299 (MQ=255)                                                                                                gaccgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgat                                                                                                                                                                       >  2:31756/1‑300 (MQ=255)                                                                                                 accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGACGCGCaggcgatc                                                                                                                                                                      >  2:452257/1‑300 (MQ=255)                                                                                                         cgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                                                                                                                              >  1:416327/1‑300 (MQ=255)                                                                                                         cgGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGGATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                                                                                                                              <  1:617207/300‑1 (MQ=255)                                                                                                                      ggCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAagag                                                                                                                                                 >  2:300576/1‑300 (MQ=255)                                                                                                                        cgagaaaagtgaggtttgttaggcgtttgAGCGCCGGGCGCGCTTCCGCCATTGCCTGCGCGAGCAGGACGGCTTAGAGCCGGATGACGTGATCCTGCCGCCGTANAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTGCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGAGCAAGGCGATCAAGCGGGAAGAGAt                                                                                                                                               <  2:709681/272‑1 (MQ=255)                                                                                                                             aCCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGTTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGc                                                                                                                                          <  1:389451/300‑1 (MQ=255)                                                                                                                                                                        ccAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcac                                                                                               <  1:513488/300‑1 (MQ=255)                                                                                                                                                                           gTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGTAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTccggcaccgg                                                                                            <  1:427582/300‑1 (MQ=255)                                                                                                                                                                                               gCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCAGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGc                                                                        >  2:381016/1‑300 (MQ=255)                                                                                                                                                                                                    gagCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCAcc                                                                   >  2:168361/1‑300 (MQ=255)                                                                                                                                                                                                      gCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGTGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTCGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTc                                                                 <  2:499413/300‑1 (MQ=255)                                                                                                                                                                                                                       tGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGTTCTGCTCCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAATGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGNGTGCGGGTGTTTGTCTCGGAAGCGCANGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATAtct                                                <  2:549818/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gggCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTAGTGGGGCAAGACGGTGTCGCtcatc                      >  1:739663/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gCTGACCTCGACGGCTTGTGTCCCGACAGGCAACATCTCACCGTAAGGTTCGGTGATCAAGGCCACGGCGATCGACCTGTCGGTGGTCGGCGAAGATGGCGTATACCACCACACCTCCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGTAAGAGATTGTGCAGGGCGATATCATGGTGGTGGTCGGCGGCGGGCTGTCCGGCACCTGCATGGAAGAGACCTATCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGTCGTCACTCATCAc                    <  2:265261/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGa                 >  1:793134/1‑300 (MQ=255)                                                                                                                                                                                                                                                        ccTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATg               >  1:22277/1‑300 (MQ=255)                                                                                                                                                                                                                                                         cTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATgc              <  2:283799/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     cTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCggg  <  1:164518/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     cTTCCCGACGGGCAACACCGCGCCGGAAGGTTCGGTGATCAAGGCTACGGCGATAGATCCGGCGGGGGGGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCNATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGTCGATATCATGGTGGTGATCGGCTGCGGGCCGTCCGGCACCGGCATGGAAGAGACCGACCAGCTCACCTCCGCGCTAAAGCATATCTCGGTGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTTTCggg  <  2:186869/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                       GACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGGG  >  NC_000913/284254‑284813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑