Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,455,999	G→T	P25P (CCG→CCT)	paaD →	phenylacetate degradation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,455,999	0	G	T	100.0%	204.7 / NA	58	P25P (CCG→CCT)	paaD	phenylacetate degradation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/33);  total (25/33)

GAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTGCCATGCCCATTTG  >  NC_000913/1455704‑1456287                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                 gAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTgaga                                                                                                                                                                                                                                                                                              >  1:50638/1‑300 (MQ=255) gAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTACAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTgaga                                                                                                                                                                                                                                                                                              >  1:332582/1‑300 (MQ=255)        gTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGt                                                                                                                                                                                                                                                                                       >  2:435230/1‑300 (MQ=255)        gTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCAGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGACAGTAAACAAGGACTGCATACCGAACACCTTGGACCCATGCTAGCAAACCTTCCGTATCTCCCGCGTGTCTTGCACGGTCAGAAATGTTAACAAAAGAGATGGATCTGCAACGTCTGACCACCATTGCACCGCCACAGGTTCATGGGATATGTGTGATACTCAGCCAGATCCCGGACCATGAGATCCCGGt                                                                                                                                                                                                                                                                                       >  2:213917/1‑300 (MQ=255)                         tgcgcTTTACGTGCAGTGTGGGAAGCCGAAGTTTTTGTCGGGATTAACGGGGCCACATTGAACGTACCGCAAGAGTAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGTTGACCATTACTGAtt                                                                                                                                                                                                                                                                      <  2:143380/296‑1 (MQ=255)                                        gCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTa                                                                                                                                                                                                                                                       >  1:778166/1‑300 (MQ=255)                                          gTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAAt                                                                                                                                                                                                                                                     <  1:352593/300‑1 (MQ=255)                                           tGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCATAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAAtg                                                                                                                                                                                                                                                    <  2:227872/300‑1 (MQ=255)                                           tGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAAtg                                                                                                                                                                                                                                                    <  1:658311/300‑1 (MQ=255)                                                 gCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGacac                                                                                                                                                                                                                                              <  2:492293/300‑1 (MQ=255)                                                           ttGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGgaga                                                                                                                                                                                                                                    <  1:302471/300‑1 (MQ=255)                                                                 ggATCAACGAAGCAGCATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAACAGGACTGCATACCGAACACTTTGGACCCATGCTGGCAGAAATGCAGTATCTACAGCGTGTATTGCCCGGTCAGCACTGGTAACAGAGGAGATGGGTATGCAACGTCTAGCAACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTAATCAGCCAGCTCCAGGCCCCTGAGATCCCGGTGCTGACCATTAATGATTTAAGCATTGCGCGTAATGTGAAACAgcttgaagaataatg                                                                                                                                                                                                                              >  2:43497/1‑286 (MQ=255)                                                                  gATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATgg                                                                                                                                                                                                                             >  1:255290/1‑300 (MQ=255)                                                                            gCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCt                                                                                                                                                                                                                   >  1:127519/1‑300 (MQ=255)                                                                                  ttGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGTAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATTAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGc                                                                                                                                                                                                             <  1:432128/300‑1 (MQ=255)                                                                                  ttGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGc                                                                                                                                                                                                             <  1:774871/300‑1 (MQ=255)                                                                                            cGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCANTGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTc                                                                                                                                                                                                   <  1:581178/300‑1 (MQ=255)                                                                                                         gTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTACAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGGTTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCa                                                                                                                                                                                      >  2:688691/1‑300 (MQ=255)                                                                                                          tATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCaa                                                                                                                                                                                     <  1:674888/300‑1 (MQ=255)                                                                                                                             aaGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTACAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGgcgc                                                                                                                                                                  >  1:381588/1‑300 (MQ=255)                                                                                                                                    gCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGt                                                                                                                                                           >  1:81338/1‑300 (MQ=255)                                                                                                                                       tACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAg                                                                                                                                                        <  2:483072/300‑1 (MQ=255)                                                                                                                                       tACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAg                                                                                                                                                        <  1:636187/300‑1 (MQ=255)                                                                                                                                       tACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATAGGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAg                                                                                                                                                        <  2:278375/300‑1 (MQ=255)                                                                                                                                         ccGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGTAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGc                                                                                                                                                      <  2:767758/300‑1 (MQ=255)                                                                                                                                                   tgtaCCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGAcaacc                                                                                                                                            <  2:6652/297‑1 (MQ=255)                                                                                                                                                                gCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGNGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGNAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTAccc                                                                                                                               <  2:752433/300‑1 (MQ=255)                                                                                                                                                                          aGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCNGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGt                                                                                                                     <  1:590368/300‑1 (MQ=255)                                                                                                                                                                            tATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGTACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTtg                                                                                                                   <  2:449504/300‑1 (MQ=255)                                                                                                                                                                              tctcCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCAGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTTATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTtgtg                                                                                                                 >  1:274396/1‑300 (MQ=255)                                                                                                                                                                               ctcCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGtgc                                                                                                                <  1:212632/300‑1 (MQ=255)                                                                                                                                                                                    gCGTGTCTTGCCCGGTCAGCAATGGGAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCAGGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCGACGGGTTTACCCCCGTTCAGGTTGTGCTGCag                                                                                                           <  1:811627/300‑2 (MQ=255)                                                                                                                                                                                        gtCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCg                                                                                                       >  2:725704/1‑300 (MQ=255)                                                                                                                                                                                                  gTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATg                                                                                             >  1:806686/1‑300 (MQ=255)                                                                                                                                                                                                     aGCAATGGTAACAGAGTAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGTGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGac                                                                                          <  2:617193/300‑1 (MQ=255)                                                                                                                                                                                                      gCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCAGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTGGATTGGCGCGATACGTGAGGCAATGACNACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGacc                                                                                         >  2:594009/1‑300 (MQ=255)                                                                                                                                                                                                         atagTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCGTTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGAACCGTCATGGaccccc                                                                                      >  2:109498/4‑297 (MQ=255)                                                                                                                                                                                                            gTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGAc                                                                                   <  1:713184/300‑1 (MQ=255)                                                                                                                                                                                                                           ggTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGAt                                                                    >  1:625508/1‑300 (MQ=255)                                                                                                                                                                                                                                              ccaTTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCANCGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCACCGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGTGTCTGCgag                                                 >  2:189995/1‑300 (MQ=255)                                                                                                                                                                                                                                              ccaTTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCgag                                                 >  1:380756/1‑300 (MQ=255)                                                                                                                                                                                                                                                aTTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGagag                                               <  1:206899/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCAt                                       <  2:5161/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aGGTTCATTAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAgcc                                  <  1:32371/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGNTGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAgcc                                  <  1:579928/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAgcc                                  <  1:506248/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAgcccg                                >  1:418834/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 tCATTAGATATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAgcccgcc                              <  1:794202/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      agaTATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTAATGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCAGACATATTCCGGCTGTCCGGCAACGGAACATTTGATTGGCGCGATANGTGAGGCAATGACAACCAACGGGTTTACCCACGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGGTGACCACCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCAAGCccgccg                         >  2:178413/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       gaTATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCgg                        >  1:360952/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       gaTATGGGCGCTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGNTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCgg                        >  1:588460/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gcgcTACTCAGCCAGATCTCGTACCCTGAGAGCCCGGTGCTGACCATTACTGATTTAGGCAGGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGATGCAATGACAACCAACGTGTTTACCCCCGTTCAGTTTGTGCTGCAACTCGACCCTGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGt                 <  2:222120/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gcgcTACTCAGCCAGATCCCGTACCCTTCGGTCTCGGGTCTTACCATTGCTGATTTAGGCACTGTGCGTAATGTCACACAGATGGTATAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGACAACGGAACATGTGATTTGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGTATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCAGACACAGt                 <  2:221242/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gcTACTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGTAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTg               <  2:104251/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aCTCAGCCAGATCCTAGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGTTTTACGCCGNCATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTGCCa            <  1:561488/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aCTCAGCCAGATCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTGCCa            <  1:341124/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aTCCCGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAANGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGNCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTGCCATGCCCATTTg  >  2:747590/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aTCCAGGACCCTGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTGCCATGCCCATTTg  >  1:485754/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                 GAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACATTTGATTGGCGCGATACGTGAGGCAATGACAACCAACGGGTTTACCCCCGTTCAGGTTGTGCTGCAACTCGACCCGGCATGGACCACCGACTGGATGACCCCCGATGCCCGTGAGCGTCTGCGAGAGTATGGCATTAGCCCGCCCGCCGGACACAGTTGCCATGCCCATTTG  >  NC_000913/1455704‑1456287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑