Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,423	(T)5→4	coding (660/1005 nt)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,423	0	T	.	100.0%	290.7 / NA	54	coding (660/1005 nt)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/34);  total (20/34)

TCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTTTATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAG  >  NC_000913/1306134‑1306720                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                          tcGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGa                                                                                                                                                                                                                                                                                                >  1:647518/1‑300 (MQ=255)   gCTGAACCACTGCGTACGTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAATGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCTAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGAcc                                                                                                                                                                                                                                                                                              <  1:73209/300‑1 (MQ=255)         ccACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGTTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATCAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCg                                                                                                                                                                                                                                                                                        <  2:281502/300‑1 (MQ=255)           aCTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTg                                                                                                                                                                                                                                                                                      >  2:13341/1‑300 (MQ=255)              gCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTa                                                                                                                                                                                                                                                                                   >  1:485706/1‑300 (MQ=255)                                         ccAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAATTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTg                                                                                                                                                                                                                                                        <  2:131424/300‑1 (MQ=255)                                               aGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTAt                                                                                                                                                                                                                                                  <  1:318470/300‑1 (MQ=255)                                                gTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTAtc                                                                                                                                                                                                                                                 >  1:69698/1‑300 (MQ=255)                                                 ttCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTAtct                                                                                                                                                                                                                                                <  1:429792/300‑1 (MQ=255)                                                            gTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCgg                                                                                                                                                                                                                                     <  1:489969/300‑1 (MQ=255)                                                                         tgCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGAc                                                                                                                                                                                                                        <  1:24415/300‑1 (MQ=255)                                                                                 gTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTatgatg                                                                                                                                                                                                                <  1:592300/300‑1 (MQ=255)                                                                                      gTTATTGCCTAACCGGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTc                                                                                                                                                                                                           <  2:329079/300‑1 (MQ=255)                                                                                            gCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACcac                                                                                                                                                                                                     <  1:169417/300‑1 (MQ=255)                                                                                            gCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCAAGCGGATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACcac                                                                                                                                                                                                     <  2:117047/300‑1 (MQ=255)                                                                                              cTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACaa                                                                                                                                                                                                   <  1:504036/300‑1 (MQ=255)                                                                                                aaCCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATc                                                                                                                                                                                                 <  1:101859/300‑1 (MQ=255)                                                                                                 aCCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATcc                                                                                                                                                                                                <  1:192114/300‑1 (MQ=255)                                                                                                        tAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTTCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGAGGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACAt                                                                                                                                                                                         <  2:323768/300‑1 (MQ=255)                                                                                                                aTCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTGTCTGGATTGCGCGTGCTCTTATTCTGGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTacac                                                                                                                                                                                 <  2:421056/300‑1 (MQ=255)                                                                                                                aTCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGCGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACTTGAGATGGGATTGTCATTAATTTG‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGGATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTacac                                                                                                                                                                                 <  2:560926/300‑1 (MQ=255)                                                                                                                 tcctcATGATTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAGTTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATGAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACAcc                                                                                                                                                                                <  2:221414/296‑1 (MQ=255)                                                                                                                 tccacATGAGTTCTCGGGTGGTCAGTGCCAGCGTATCAGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCGACCACAATCCACTACATCCTTACAcc                                                                                                                                                                                <  1:498307/296‑1 (MQ=255)                                                                                                                 tCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACAcc                                                                                                                                                                                <  1:392107/300‑1 (MQ=255)                                                                                                                             ctctGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATg                                                                                                                                                                    <  2:360867/300‑1 (MQ=255)                                                                                                                                  gtggGCAGGGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGAGGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGc                                                                                                                                                               <  1:262785/300‑1 (MQ=255)                                                                                                                                              caggTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCNGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑CTCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  2:573349/297‑1 (MQ=255)                                                                                                                                              aGCGTATCGTGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  2:386395/300‑1 (MQ=255)                                                                                                                                              aGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGTTCAACCTACTCCAGCAATTGCAACGTGAGGTTGGATTGTCATTAATTTT‑ATCGCTCATAACCTGGCCGTGGTAAAACACATTTCCAATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  2:173422/300‑1 (MQ=255)                                                                                                                                              aGCGTATCGGGATTGCGCGTGCCCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  2:181892/300‑1 (MQ=255)                                                                                                                                                 gTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAccctg                                                                                                                                                >  1:584164/1‑297 (MQ=255)                                                                                                                                                       gggttGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGNCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGGTGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCNACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGa                                                                                                                                          >  2:612478/4‑300 (MQ=255)                                                                                                                                                              gcgTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTTAGATTGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGNGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTNGGCAGTCCCCATACCTGATCCGGATCTGgag                                                                                                                                   <  2:464281/300‑1 (MQ=255)                                                                                                                                                                         ttCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAAc                                                                                                                        <  2:226852/300‑1 (MQ=255)                                                                                                                                                                                        tGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGa                                                                                                         <  1:405723/300‑1 (MQ=255)                                                                                                                                                                                                   gatgaGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTGCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTAGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTAc                                                                                              >  2:412281/1‑300 (MQ=255)                                                                                                                                                                                                   gatgaGCCGGTGTCGGCGCTGGACGTGGCAATTCAGGCACAGTTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTAc                                                                                              <  2:250721/300‑1 (MQ=255)                                                                                                                                                                                                                  gcgcTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAAcc                                                                               >  2:138191/1‑300 (MQ=255)                                                                                                                                                                                                                  gcgcTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAAcc                                                                               >  1:292240/1‑300 (MQ=255)                                                                                                                                                                                                                  gcgcTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTAGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAAcc                                                                               >  1:122397/1‑300 (MQ=255)                                                                                                                                                                                                                            tgtCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCgg                                                                     >  2:617060/1‑300 (MQ=255)                                                                                                                                                                                                                                  ttCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTgtgt                                                               <  1:630283/300‑1 (MQ=255)                                                                                                                                                                                                                                     aGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGAACTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTAACACAATCCACTACATCTTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCAGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGCTCAACCCGCCCTCCGGTTGTGtttt                                                            >  2:254452/1‑300 (MQ=255)                                                                                                                                                                                                                                          caGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTa                                                       >  1:161778/1‑300 (MQ=255)                                                                                                                                                                                                                                          caGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGAGCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTa                                                       >  2:557518/1‑300 (MQ=255)                                                                                                                                                                                                                                             gtggtCAACCTGCTCCAGCAATTTCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGANGTATCTCGGCCATGCGGTAGAACTGTGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTAccc                                                    <  2:568698/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aCCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGccc                                             >  2:553960/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGAtt                                         >  1:11400/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCagag                             <  1:13354/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     cAACGTTAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGt                            <  2:219641/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTgcg                         >  2:558208/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aTGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCaaaa                   >  1:642378/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 ggATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAacac                >  1:623615/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCt           >  1:369735/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                tttATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGNTGTATCTCGTCCATGCGTTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAg  <  2:492977/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                          TCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTTTATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAG  >  NC_000913/1306134‑1306720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑