Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,870	0	C	A	27.8%	98.1 / 20.1	54	S54S (TCC→TCA)	ychS	putative uncharacterized protein YchS
Reads supporting (aligned to +/- strand):  ref base C (15/24);  new base A (12/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCT  >  NC_000913/1286575‑1287159                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   gCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAG‑TTCCgggg                                                                                                                                                                                                                                                                                                >  1:98845/1‑300 (MQ=255) gCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCgggg                                                                                                                                                                                                                                                                                                >  1:429470/1‑300 (MQ=255)  cagggTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGNAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAgggac                                                                                                                                                                                                                                                                                               <  2:677248/297‑3 (MQ=21)     ggTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAgggacctg                                                                                                                                                                                                                                                                                            >  1:690219/1‑295 (MQ=21)      gTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTAATTGCGGATTCGTTTGAAAA‑TTCAGGGACtttt                                                                                                                                                                                                                                                                                           >  1:760965/1‑300 (MQ=255)      gTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCACGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCAGGGGCtttt                                                                                                                                                                                                                                                                                           >  1:376522/1‑300 (MQ=255)      gTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGAGATTGTAGGCACGGTAAAAGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCTCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTAATCCTTCCCCGCTTGAGCAGAATATTTGATTGCGGATTCGTTTGCGAA‑TTCAGGGGAtttt                                                                                                                                                                                                                                                                                           >  2:7348/1‑300 (MQ=25)                 gTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGAtgg                                                                                                                                                                                                                                                                                >  2:285203/1‑300 (MQ=255)                    gTCGGATGCGCTTCGCTTATCCGACCTACAGTGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAATCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGAtggtgg                                                                                                                                                                                                                                                                             <  1:780839/300‑1 (MQ=255)                    gTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGAtggtgg                                                                                                                                                                                                                                                                             <  1:650519/300‑1 (MQ=255)                         aTGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTgggg                                                                                                                                                                                                                                                                        <  1:198526/300‑1 (MQ=255)                            cgcttcgcttATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGaa                                                                                                                                                                                                                                                                     <  1:734192/300‑1 (MQ=255)                                tcgcttATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTG‑GAAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                                                                                                                                                                                                                                                 >  1:299353/1‑300 (MQ=255)                                tcgcttATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                                                                                                                                                                                                                                                 >  2:713584/1‑300 (MQ=255)                                tcgcttATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAAAAGGTTTTGATTATTTAAGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                                                                                                                                                                                                                                                 >  1:299210/1‑300 (MQ=17)                                     tATCCGACCTACATGTTATGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGTAAGCGATGGTGGTGGGGTAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc                                                                                                                                                                                                                                                            <  2:416443/300‑1 (MQ=255)                                     tATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttcgcc                                                                                                                                                                                                                                                            >  1:759760/1‑296 (MQ=17)                                          gatctacatttgagcatagtGTATGCCCGGTAAACGTGGTGTCAGCGGGCAAAATTAGCTAGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                                                                                                                                                                                                                                       <  2:199760/281‑1 (MQ=17)                                          gaccgaaaggtggggagagtGTAGGCCCGGTAAACGTGGTGCCATAGGGCAAAATTAGCTCGGAGTAACAGGGTTTGATTGTTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCNCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAANTGATGGTGGTGGGGGGAGGATTACTCAgcgc                                                                                                                                                                                                                                                       <  2:551894/281‑1 (MQ=14)                                          gACCTACAGGGTAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                                                                                                                                                                                                                                       <  1:331045/300‑1 (MQ=255)                                          gACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGTTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                                                                                                                                                                                                                                       <  1:329312/300‑1 (MQ=255)                                           aCCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCNCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCNTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttcgaaccttcg                                                                                                                                                                                                                                                      >  2:614145/1‑290 (MQ=17)                                           aCCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTT‑GGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttcgaaccttcg                                                                                                                                                                                                                                                      >  1:300574/1‑290 (MQ=14)                                           aCCTAAAGGGGAGGATATTGTAGGCCCGGTAACCGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGCTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCAGCATAAGCAGCTCAGGTAATCGCGGATGCGTTCGAGAA‑TTCAGGGACTTTTGAAAGTGCTGGTGGTGAGGGAAGGATTACGCAgcgat                                                                                                                                                                                                                                                      >  1:742551/1‑298 (MQ=18)                                           aCCTAAAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCACCGCATGGGCAGAATATTTAATTGCGGATTCGTTTGGAAA‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttccccctctct                                                                                                                                                                                                                                                      >  2:434463/1‑290 (MQ=17)                                               aCAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgc                                                                                                                                                                                                                                                  >  1:155870/1‑300 (MQ=255)                                                            ttGTAGGCCCGGTCAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTAATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCCTCCGCTCTGCGTGCTTCGCCCTTCg                                                                                                                                                                                                                                     >  1:233666/1‑300 (MQ=255)                                                               tAGGCCCGGTAAACTTGGTGCCATCGGTCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGTTGGTGGGGGAAGGATTACTCAGCACTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCTGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGt                                                                                                                                                                                                                                  <  1:77183/300‑1 (MQ=255)                                                               tAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGt                                                                                                                                                                                                                                  <  1:77056/300‑1 (MQ=255)                                                                aGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGTAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGTGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                                                                                                                                                                                                                                 <  2:295247/300‑1 (MQ=255)                                                                     cGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCCCCCTCGCTGCGCGCTTCACCCTCCGGGTCGTTGc                                                                                                                                                                                                                            >  1:729906/1‑300 (MQ=255)                                                                      agTAAACGTGGTGCCATCGTGCAAAATTAGCTAGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGTGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGTTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc                                                                                                                                                                                                                           <  2:350711/299‑1 (MQ=255)                                                                       gTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                                                                                                                                                                                                                          >  1:292787/1‑300 (MQ=255)                                                                                         ggCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGc                                                                                                                                                                                                        <  1:249215/300‑1 (MQ=255)                                                                                             aaaTTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGNTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGAc                                                                                                                                                                                                    <  1:572030/300‑1 (MQ=255)                                                                                                        gAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCGTCGCTTCGCGCCTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCACCCTTCCCCGATGGTGTCGAGTATATGCTTGCAAGGTCGCACGAGAA‑GTCCGAGGATCTTGAGAGTGATGGTGGTGGGGGGAGGAGTAAGCAGCGCTGAGTGCTTAGCACTTAGGGTCGGTGACTGCGGCCACGCTCTCTAGCTGACGCCCGAGCCGa                                                                                                                                                                                         >  1:243450/1‑300 (MQ=9)                                                                                                                              aaggagtcGATGGTGTTGGGGGAAGGATTGCTCAGCGCTGCGCGTTTCTCCCTTCGTGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCTNGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                                                                                                   <  2:196957/293‑1 (MQ=17)                                                                                                                               atgAAGCGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCTCCGCGTGGGCAGAATATGGGATTGCGGATTCGGTTGAGAA‑TTCCGGGGCTGTGGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                                                                  <  1:500263/298‑1 (MQ=14)                                                                                                                               aGGAATCGATGGTGGTTGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGTGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTGTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATcc                                                                                                                                                                  <  2:212115/300‑1 (MQ=255)                                                                                                                                 gAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCtt                                                                                                                                                                >  1:209488/1‑300 (MQ=255)                                                                                                                                        tggtggttgGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAATTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                                                                         <  1:470906/300‑1 (MQ=255)                                                                                                                                                       gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTg                                                                                                                                          <  1:328932/300‑1 (MQ=255)                                                                                                                                                         ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGAt                                                                                                                                        <  1:719185/300‑1 (MQ=255)                                                                                                                                                           aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGTGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCACCGCATGGGCAGAATAGTCGATTg                                                                                                                                      >  1:167088/1‑300 (MQ=255)                                                                                                                                                                       gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCAGAACCTTAGTCGAAGCTTCTAATCCTTCCCCGAATGGGCAGAATATTTGATTGCGGATTCGCTTg                                                                                                                          >  1:680304/1‑300 (MQ=255)                                                                                                                                                                        cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCAGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                                                                         >  1:288493/1‑300 (MQ=255)                                                                                                                                                                            gcgagatgtgtataagagacagCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCCTCCGCTCTGCGCGCTTAGCCCTTCGGGTCGTTGCCTACAACCCCGCTCTCTCGCTGACGCTCAAGCCGAACCTTAGTCGCCGCTTCTCATCCTTCCCCGCATGGGCAGAAGAGTCTACTGCGGATTCGCTTgaacgt                                                                                                                     >  1:392842/22‑296 (MQ=17)                                                                                                                                                                                                        cgttctcttGCTGGCGCTCAAGTCGAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAAGGTGTGAGTGCGGATTCGTTGGAGAA‑TTCCGGGGTTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGAtggtggtg                                                                                        <  2:600576/297‑1 (MQ=17)                                                                                                                                                                                                                       gcTCAGGTCGAACCTTGTTCGGAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAATTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGTAAGGATTACTc                                                                         <  2:334678/300‑1 (MQ=255)                                                                                                                                                                                                                       gcTCAAGTCGAACCTTGGTCGAAGCTTCGCACCCTTCCACGCGTTGGCAGAATATCTGATGGCGGATTCGTCTTAGGG‑TTTCGGGGTTTGTGAAAGTGATGGTGGTGGGGGGAGGACTGCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTCCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTc                                                                         <  2:7635/300‑1 (MQ=17)                                                                                                                                                                                                                             gTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCCTCCGCGCTGCGTGCTTAGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTAGAACCTTAGTCGCAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTTCGGATTCTCTTGA‑AGATTCATTGACTTTTGCAAGTGCTGGTGTTTAAGGACGGATTACCCAGCGCt                                                                   >  1:58956/1‑300 (MQ=14)                                                                                                                                                                                                                                   ccTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcgc                                                             <  1:694220/300‑1 (MQ=255)                                                                                                                                                                                                                                               cTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCTCTGCGCGCTTAGCCCTTCgg                                                 >  1:65519/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              tctagaA‑TTCCGTGGCTTGTGAAAGTGATAGCGGTGGGCGAAGGACTACTCAGTGCTGCGCGCTTCGCCCTTCGTGGCGTTGCCTGCGGCAGCGCTCTCTCGCTGACGCTCGAGCCGAACTTTGGTCGAAGTTTCTCATCCTTCCCCGCAGGGGCAGAATATGTGATTGCGGATTAGCTGGAGAG‑TTCAGTGACTTGGGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCt  <  1:366409/297‑1 (MQ=17)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   GCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAA‑TTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAG‑TTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCT  >  NC_000913/1286575‑1287159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑