Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,949	T→C	noncoding (357/363 nt)	ssrA →	tmRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,949	0	T	C	97.7%	301.6 / NA	87	noncoding (357/363 nt)	ssrA	tmRNA
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base C (36/48);  minor base A (2/0);  total (38/49)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGG  >  NC_000913/2755672‑2756241                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        aaaaaTAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGTAAGCCCNGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTg                                                                                                                                                                                                                                                                                   <  2:146856/300‑1 (MQ=255)       aGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATNGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCNTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTAcc                                                                                                                                                                                                                                                                             <  2:497945/300‑1 (MQ=255)           gCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCagag                                                                                                                                                                                                                                                                         <  1:552864/300‑1 (MQ=255)            cAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGt                                                                                                                                                                                                                                                                        <  2:63600/300‑1 (MQ=255)            cAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGt                                                                                                                                                                                                                                                                        <  1:513243/300‑1 (MQ=255)                      aaaCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGNTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGGCCGTCCTCAACTTGCAAGCGAATGT‑AAAGACCGACCAAACATGTAGTACCGAGGAGGTAAGAACTTCTGAAGCGGGGTCC‑ANCCCCACCAACACCACCAAAACTCTCCCCCGGTGATTACCAGAGTCATCCCATGa                                                                                                                                                                                                                                                              >  2:590356/1‑300 (MQ=255)                      aaaCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCNGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTNTCCATCGGTGATTACCAGAGTCATCCGATGa                                                                                                                                                                                                                                                              <  2:451475/300‑1 (MQ=255)                       aaCTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGGTCGTCCGCAACTGGCAAAAGAAGGT‑AAAAAATGAACAAAAACGTAGTACCAAGGAGGTAGGAAGTTCGGACGAAGGTTTC‑ACCCCCACCAGCCCCACCAAAACTCTCCACCAGTGAttccccaagtcattccatgaa                                                                                                                                                                                                                                                             >  1:60078/1‑281 (MQ=255)                               tttAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCNGGCTAGTTTGTTAGTTGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTTGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aag                                                                                                                                                                                                                                                   <  2:146735/300‑1 (MQ=255)                               tttAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aag                                                                                                                                                                                                                                                   <  1:193731/300‑1 (MQ=255)                               tttAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aag                                                                                                                                                                                                                                                   <  2:22537/300‑1 (MQ=255)                                ttAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAATAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCANGCTAGTTTGTTATTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aaga                                                                                                                                                                                                                                                  <  2:145867/300‑1 (MQ=255)                                 tagtagcTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCTAGGATGTAGGAATTTCGTACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aagag                                                                                                                                                                                                                                                 <  2:160005/296‑1 (MQ=255)                                 tAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAATAGATTGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aagag                                                                                                                                                                                                                                                 <  1:262622/300‑1 (MQ=255)                                 tAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑Aagag                                                                                                                                                                                                                                                 <  1:359357/300‑1 (MQ=255)                                 tAGCAGCTTAATAACCTACTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAATAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTTGGGTTGAAGCGTGAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTATCCGTTCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATTCGATGAAGT‑CCT‑Aagag                                                                                                                                                                                                                                                 <  2:19468/300‑1 (MQ=255)                                   gcagcTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTACGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGcc                                                                                                                                                                                                                                               <  1:147821/300‑1 (MQ=255)                                        ttAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCAc                                                                                                                                                                                                                                          <  1:122949/300‑1 (MQ=255)                                             aaCCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCTGTCCTCCACTGGCAAGCGAACGT‑CAAAAATGACTCAGCCTGTCGTACCGAGGATGTAGGAATTTCGGGAGCGGGTTTC‑ACTCCCGCCAGCCCCAACCAAACTCTCCCTCGGTGATTCCCAAAGTCCTCCGATGGAGT‑CCTCAAG‑GCCCGAACGgcgc                                                                                                                                                                                                                                     >  2:315794/1‑300 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGTTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑cc                                                                                                                                                                                                                               <  1:183510/300‑1 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑cc                                                                                                                                                                                                                               <  1:50685/300‑1 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAAAGAATGT‑AAAAACCGACTCAACATGTAGTCCCAAGGATGTAAGAACTTCTGACGCGGGTTCA‑ACCCCCACCCGCCCCACCAAAACTCTCCACCGGTGGTTCCCCGAGTTATCCGATGAAGG‑CCT‑CAGGGCCCGCaaggtgtaagac                                                                                                                                                                                                                                >  1:72378/1‑289 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAACTGGCCAGCGAACGT‑AAAGACCGACCCAGCACGTAGTACCAAGGAGGTAGGAACTTCTGACGCGGGTTCC‑ACTCCCACCCGCCCCACCAAAACTCTCCCCCGGTGATTCCCAAAATCATCCGATTAAAT‑CCT‑CAAAGCCCACACAGAGCAAG‑cc                                                                                                                                                                                                                               >  1:361448/1‑300 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAACTGGCAAGCGAACGT‑AAAGACTGACCCAGAATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCC‑CCTCCCGCCAGCCCCACCAAAACTCTCCCCCGGTGATTCCCCAAGTCATCCGATGAAGT‑CCT‑CAGAGCCCGCCCGGCGCAAG‑cc                                                                                                                                                                                                                               >  1:397148/1‑300 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAACTGGCAAGCAAATGT‑AAAAACCGACTCAACACGTCATACCGAAGAGGTAGGAAATTCGGACGCGGGTTTC‑ACTCCCACCAACCCCCCCAAAACTCCCCAGCAGTGATTACCCAAGGCATCCGATGAAGT‑CCtcaaagaccacacagagcaagcc                                                                                                                                                                                                                                 >  1:215702/1‑278 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGAAACTGGCAAGCGAATGT‑AAAAACCGACTCAACACGTAGTCCCAAGGATGTAGGAATTTCGGACGCGGGTTTC‑ACTCCCACCAGCCCCCCCAAAACTCTCCCTCGGTGATTCCCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAgac                                                                                                                                                                                                                                >  1:411817/1‑298 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCATCCACAACTGGCCAACGAACGT‑CAAAACTGACCCAGCACATCGTCCCAAAGATGTAAGAATTTCTGACGCGGGTTCC‑ACCCCCCCCAACCCCACCCAAACTCTCCACCTGTGACTCCCCAAGTCATCCAACGAAGT‑CCC‑CAGAGCCCacacagcaccaacc                                                                                                                                                                                                                                >  1:591600/1‑290 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTGAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑cc                                                                                                                                                                                                                               <  1:334914/300‑1 (MQ=255)                                                  gCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCNTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGNTAGTGGCGTGTTCGTCCTCCACTGGCAAGCGAAGGT‑AAAAAATGACTCAACCTGTCGTCACAAGGATGTAGGAATCTTTGATACGGGTTCCCAC‑CCCTCCAGCCCCACCAACACTCTCCCCCTGTGATTAACAGAAGCATCCGATGAAAT‑CAT‑CAGAGCCCCCACAGCGCAAG‑cc                                                                                                                                                                                                                               >  2:442989/1‑300 (MQ=255)                                                                ctcCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCCACTGGCAAGCGAATGT‑AAAGACCGACTCAGCATGTCGTACCGAGGATGTAGGAATTTCTAACGCGGGTTCC‑ACCCCCACCCGCCCCCCCCAAACTCCCCCTCGGTGGTTACCAAAGTCATCCGATGAAGT‑CCT‑CAGAACCCGCCCGGCGCCAA‑CCCTTCTGGCtttttt                                                                                                                                                                                                                 >  1:111197/1‑300 (MQ=255)                                                                 tcCCTAGCCTCCGCTCTTAGGACGGGGATCANGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCANCCACCACCAGCAAACGAACGT‑AAAAAATGACTAAGAACATAGGACCAAGGATGTAGGAACTTCGGACGCGGGTTTA‑ACCCCCGACAGCACCCCCAAAACTCTCCACCAGTGGTTCCCCAAGTCATCCGATGAAGTACCC‑AA‑AGCCCGCACggggtaaacccctttgggttttttt                                                                                                                                                                                                                 >  2:444462/1‑277 (MQ=255)                                                                        ccTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCATGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTgtctcttatacacatctcgc                                                                                                                                                                                                         <  1:646063/300‑20 (MQ=255)                                                                           ccGCTCGTAGGACGGGGATCAAGAGAGGTCAAATCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGTTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCACCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAAt                                                                                                                                                                                                      <  2:205153/300‑1 (MQ=255)                                                                            cGCTCTTAGTACGGGGATCAATAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTCAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAAtt                                                                                                                                                                                                     <  2:546943/300‑1 (MQ=255)                                                                              ctctTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCCGCTGGCAAACGAATGT‑AAAGACTGACTCAGCATGTCGTCCCAAGGATGTAGGAATTTCTGACGCGGGTTCC‑ACCCCCGCCCGCCCCCCCAAAATTCTCCACCGGTGGTTCCCAGAGTCATCCGATGAAGT‑CCT‑CAGAGTCCGCACGGCGCAAG‑CCCTGCGGGTTTTTTTTTGCCCTCCAtttt                                                                                                                                                                                                   >  1:550243/1‑299 (MQ=255)                                                                                 ttAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTNCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTcc                                                                                                                                                                                                <  1:464763/300‑1 (MQ=255)                                                                                           gATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAACTGGCAAGCAAATGT‑AAAAACTGACTCAGCACGTAGTACCAAAGATGTAGGAATTTCTGACGCGGGTTCC‑ACCCCCACCAACCCCNCCAAAACTCTCCCCCTGTGGTTCCCAAAGTCATCCGATGAAGT‑CCT‑CAGAGCCCTCACGGCGCAAG‑CCCTTCGGGCTTTTTTTTGCCCTCAATTTGTCCCTCGAAGTcc                                                                                                                                                                                      >  1:460096/1‑300 (MQ=255)                                                                                           gATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCACCACTTGCAAACGAACGTCAAANCCT‑ACCCCACATGTCGTCCCGAGGATGTCGGAATTTCGGACACGGGTTTC‑ACTCCCCCCAANCCCCCCCAAACTCTCCCCCTGTGATTCCCCGAGTCCTCCGATGAAGT‑CCT‑CAGAGCCCGCACGGAGCAAG‑CCCTGCGGGCTTTTTTTTGCCCTCAAGTTGTCCCAAGAAGTcc                                                                                                                                                                                      >  2:504164/1‑300 (MQ=255)                                                                                                agagagGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCATCCGCAACTGGCAAACGAATGT‑AAAGAATGACTCAGCACGTCGTACCGAGGATGTAGGAACTTCGGACGCGGGTTCC‑ACTCCCACCCACCCCACCAAAACTCTCCCCCAGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGACCGCACGGCGCAAG‑CCCCGCGGGCTTTTTTGTGCCCCCAACCTTTCCCGCGAAGTCCGAaga                                                                                                                                                                                 >  1:372235/1‑300 (MQ=255)                                                                                                           aCCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCCACTGGCAAGCGAATGT‑CAAAACCGACTAAGCATGTCGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCC‑ACCCCCACCAACCCCACCAAAACTCTCCCTCGGTGATTCCCCGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTTCGGGCTTTTTTTTGTCCTCCACTTTTCCCACAAAGTCCGAAGAGAAATAAttca                                                                                                                                                                      >  1:640003/1‑298 (MQ=255)                                                                                                              cAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATc                                                                                                                                                                   <  1:205004/300‑1 (MQ=255)                                                                                                                   gagaCCGCGTGGAAGCCCTGCCTTGGTTTGAAGCGTTAAAACTTAATCATGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCACACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAAc                                                                                                                                                              <  2:108732/300‑1 (MQ=255)                                                                                                                        cgcgTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCCTCCGCCAATTGCCAGAGAACGT‑AAAAACTGACTTAACACGGAATCCCAAGGATGTAGGAACTTCTGACGCAGGTTCA‑CCCCCCACCAACCCCAACAAAACTCTCCATCCGTGATTCCCAAAGTCATCCGATGAAGC‑ACT‑AAGAGCCCGCACGGCGCAAG‑ACCTGCGGGCTTTTTTGTGTCCTCCATTTGTTCCACTAAGTCTGAAGAGAACTCATTGAATCCGAACCtttt                                                                                                                                                         >  2:263832/1‑300 (MQ=255)                                                                                                                            tGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTCTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTatgc                                                                                                                                                     <  2:211733/300‑4 (MQ=255)                                                                                                                             ggAAGCCCTGCCTGGGTTTGAAGCGTTAAAACTTAATCATGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGTTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGcc                                                                                                                                                    <  2:347355/300‑1 (MQ=255)                                                                                                                             ggAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGcc                                                                                                                                                    <  1:618575/300‑1 (MQ=255)                                                                                                                             ggAAGCCCTGCATGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGcc                                                                                                                                                    <  2:23253/300‑1 (MQ=255)                                                                                                                                    ctgcctgGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTATTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGAt                                                                                                                                             <  2:388020/300‑1 (MQ=255)                                                                                                                                    ctgccggGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCTTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCTAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGAt                                                                                                                                             <  2:127255/300‑1 (MQ=255)                                                                                                                                      gcctgGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCCACTGGCCAACAAACGTCAAAAACT‑ACCCAACACGTAGTACCAAAGATGTCAGAACTTCTGACGCAGGTTCC‑ACCCCCACCAGCCCCCACAAAATTCTCCACCGGTGATTCCCCGAGTTCTCCTACGAAAG‑CCT‑CAGAGCCCGCCCGGTGCCAGACCCT‑CGGGCTTTTTTTTTTCCTCCATTTGTCCCACGAAATTCGAAAAGAAATGACTCAATCCGAAACTTTTAGGGCCAtttgtag                                                                                                                                           >  1:137546/1‑295 (MQ=255)                                                                                                                                         tgGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGcc                                                                                                                                        <  1:503676/300‑1 (MQ=255)                                                                                                                                                           aCTTAATCATGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTNA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTg                                                                                                                      <  1:450770/300‑1 (MQ=255)                                                                                                                                                              tAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGtgt                                                                                                                   <  1:72360/300‑3 (MQ=255)                                                                                                                                                                  cAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGt                                                                                                               <  1:468336/300‑1 (MQ=255)                                                                                                                                                                   atgCTAGTTTGTTAGTGGCGCGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAAGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACTGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGtt                                                                                                              <  2:16115/298‑1 (MQ=255)                                                                                                                                                                       tAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCANCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGc                                                                                                          <  1:465826/300‑1 (MQ=255)                                                                                                                                                                            tGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTaaa                                                                                                     <  1:497720/300‑1 (MQ=255)                                                                                                                                                                             gTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCANGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAAc                                                                                                    <  2:468399/300‑1 (MQ=255)                                                                                                                                                                                   ggCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTCATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAggg                                                                                              >  2:277467/1‑300 (MQ=255)                                                                                                                                                                                    gCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGga                                                                                             <  1:365301/300‑1 (MQ=255)                                                                                                                                                                                     cGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGgag                                                                                            <  2:538746/300‑1 (MQ=255)                                                                                                                                                                                      gtgtCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCNATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGgaga                                                                                           >  1:423507/1‑300 (MQ=255)                                                                                                                                                                                       tgtCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGNGCCCGCACGGCGCAAG‑CCCTGCGGGCNTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGAc                                                                                          <  2:422649/300‑1 (MQ=255)                                                                                                                                                                                            gtccgCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTcccc                                                                                     >  1:344156/1‑300 (MQ=255)                                                                                                                                                                                              ccgCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGCGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCAt                                                                                   <  1:107591/300‑1 (MQ=255)                                                                                                                                                                                                                       gactgactAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAAc                                                           <  1:584267/300‑1 (MQ=255)                                                                                                                                                                                                                               aaGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGCTAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACgg                                                   >  2:133464/1‑300 (MQ=255)                                                                                                                                                                                                                                aGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGa                                                  >  1:517167/1‑300 (MQ=255)                                                                                                                                                                                                                                 gCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGaa                                                 >  1:356215/1‑300 (MQ=255)                                                                                                                                                                                                                                   aTGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTTTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAAt                                               >  1:269106/1‑300 (MQ=255)                                                                                                                                                                                                                                   aTGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAGGCCGTTAACTGATACGGAAAt                                               >  1:61640/1‑300 (MQ=255)                                                                                                                                                                                                                                   aTGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAAt                                               >  1:146381/1‑300 (MQ=255)                                                                                                                                                                                                                                   aTGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCACATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAAt                                               >  1:271711/1‑300 (MQ=255)                                                                                                                                                                                                                                   aTGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCAGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAAt                                               >  1:53544/1‑300 (MQ=255)                                                                                                                                                                                                                                   aTGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CACTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCATAAACGCAGGGAGACTCACCATGGCAAGGAAAACCAAGCCGTTAACTGATACGGacgt                                               >  2:79962/1‑297 (MQ=255)                                                                                                                                                                                                                                     gtagtaCCGAGGATTTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCa                                             <  2:84028/300‑1 (MQ=255)                                                                                                                                                                                                                                     gtagtaCCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCa                                             >  1:584262/1‑300 (MQ=255)                                                                                                                                                                                                                                       agtaCCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCaaa                                           >  1:541918/1‑300 (MQ=255)                                                                                                                                                                                                                                          aacGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACNAATTAAATCCGAACCTTTTAGGCACATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCATAAACGCAGGGAGCCTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAgta                                        >  2:157759/3‑298 (MQ=255)                                                                                                                                                                                                                                                    tAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTa                              >  1:556343/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAg                           <  1:403690/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aaTTTCGGACGCGGGTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGa                          <  1:502886/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     ggTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAg             >  1:126621/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     ggTTCA‑ACTCCCGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCAGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAg             >  1:390817/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               cccGCCAGCTCCACCAAAATTCTCCAGCGGTGAGTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTTCCGCTAAGTCCTAAGAGAACTAATTAAATCCGAACCCTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAAATGCCGATTACCAGCTTTAtgac    <  2:54395/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               cccGCCAGCCCCACCAAAATTCGCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCGAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGTCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgac    <  2:54539/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cGCCAGCCCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTAtgacgg  >  2:581582/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        AAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCGCAGCTGGCAAGCGAATGT‑AAAGACTGACTAAGCATGTAGTACCGAGGATGTAGGAATTTCGGACGCGGGTTCA‑ACTCCCGCCAGCTCCACCAAAATTCTCCATCGGTGATTACCAGAGTCATCCGATGAAGT‑CCT‑AAGAGCCCGCACGGCGCAAG‑CCCTGCGGGCTTTTTTGTGCCCTCAATTTGTCCCGCGAAGTCCGAAGAGAACTAATTAAATCCGAACCTTTTAGGCCCATTGATAGGCCCAACGAAAAGCTCTATTGTTTACGTTGGGCCTAAACGCAGGGAGACTCCCCATGGCAAGAAAAACCAAGCCGTTAACTGATACGGAAATCAAAGCCGCCAAACCTAAAGATGCCGATTACCAGCTTTATGACGG  >  NC_000913/2755672‑2756241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑