Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,423	(T)5→4	coding (660/1005 nt)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,423	0	T	.	100.0%	282.1 / NA	54	coding (660/1005 nt)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/32);  total (22/32)

GCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTTTATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAG  >  NC_000913/1306136‑1306720                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                          gCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGGCGGGTTATTGCCTAACCTGATTAACCGCGAGAAGCAGGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGAcc                                                                                                                                                                                                                                                                                              <  2:272426/300‑1 (MQ=255)  cTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCt                                                                                                                                                                                                                                                                                             >  2:226894/1‑300 (MQ=255)     aaCCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGc                                                                                                                                                                                                                                                                                          <  1:367450/300‑1 (MQ=255)     aaCCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATNGCGCGTGCTCTTATTCTTGAACCGAANCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGc                                                                                                                                                                                                                                                                                          <  2:413011/300‑1 (MQ=255)       ccACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCg                                                                                                                                                                                                                                                                                        <  1:489092/300‑1 (MQ=255)            gCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGTGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCATAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGTTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTa                                                                                                                                                                                                                                                                                   >  1:514411/1‑300 (MQ=255)                                       ccAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCTTGGTAAAACACATTTCCGATCGTGTGTTGGTg                                                                                                                                                                                                                                                        <  1:510200/300‑1 (MQ=255)                                              gTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCNCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGNTGTAtc                                                                                                                                                                                                                                                 >  2:524195/1‑300 (MQ=255)                                               ttCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCATAACCTGATTAACCACTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATTGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTAtct                                                                                                                                                                                                                                                <  2:268508/300‑1 (MQ=255)                                                  gcgAGCGCGTGAAGGCGATGATGCTGAAATTCGGTTTATTGCCTAACCTAATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCgg                                                                                                                                                                                                                                             <  2:50459/300‑1 (MQ=255)                                                         cgTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCNGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTAGGCCATGCg                                                                                                                                                                                                                                      >  2:557078/1‑300 (MQ=255)                                                         cgTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCg                                                                                                                                                                                                                                      >  1:368241/1‑300 (MQ=255)                                                                gCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAAc                                                                                                                                                                                                                               <  1:257645/300‑1 (MQ=255)                                                                  gatgatGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTg                                                                                                                                                                                                                             <  1:347804/300‑1 (MQ=255)                                                                        gCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCGGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGTACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGAcc                                                                                                                                                                                                                       <  1:199738/300‑1 (MQ=255)                                                                        gCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGAcc                                                                                                                                                                                                                       <  1:176105/300‑1 (MQ=255)                                                                               gTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTatgatg                                                                                                                                                                                                                <  1:130834/300‑1 (MQ=255)                                                                                         tGCCTAACCTGATCAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCTAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACGGGGGACCTATGATGAGGTCTACca                                                                                                                                                                                                      <  2:230758/300‑1 (MQ=255)                                                                                          gCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACcac                                                                                                                                                                                                     <  1:469882/300‑1 (MQ=255)                                                                                          gCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGGGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACcac                                                                                                                                                                                                     <  2:39470/300‑1 (MQ=255)                                                                                            cTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACaa                                                                                                                                                                                                   >  1:81060/1‑300 (MQ=255)                                                                                                           gCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTa                                                                                                                                                                                    >  1:37069/1‑300 (MQ=255)                                                                                                               tCCGCATGCGTTCTCTGGTGGGCAGTGCCAGCGGATCGGGATGACGCGGACTCTTATTCTGGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAATCTGCTCCAGCAATGGCAACGTGAGGTGGGACTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGTTGATGTATCTCGGCCATACGGTAGAACTGGGGACCTATGATGAGCTCTACCACAATCCACTACATCCCTACAcc                                                                                                                                                                                <  1:147265/300‑1 (MQ=255)                                                                                                                    aTGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGc                                                                                                                                                                           <  1:195663/300‑1 (MQ=255)                                                                                                                        gTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACNTGCTCCAGCAATTGCAACGTGAGATGNGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTg                                                                                                                                                                       >  2:422509/1‑300 (MQ=255)                                                                                                                        gTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTg                                                                                                                                                                       >  1:204215/1‑300 (MQ=255)                                                                                                                             ctGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTc                                                                                                                                                                  >  1:136143/1‑300 (MQ=255)                                                                                                                               ggtggGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGTGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCCCTACATCCTTACACCAGGGCATTGATGTCgg                                                                                                                                                                >  2:608756/1‑300 (MQ=255)                                                                                                                                gtggGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACATATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGc                                                                                                                                                               <  1:552016/300‑1 (MQ=255)                                                                                                                                gtggGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGGAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGc                                                                                                                                                               <  1:466635/300‑1 (MQ=255)                                                                                                                                  gggCAGTGCCAGCGTATCGGGATTGCGCGTGNTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCNATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAg                                                                                                                                                             >  2:443435/1‑300 (MQ=255)                                                                                                                                       gTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTcccc                                                                                                                                                        >  2:394414/1‑300 (MQ=255)                                                                                                                                       gTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTcccc                                                                                                                                                        >  1:388124/1‑300 (MQ=255)                                                                                                                                            agtgtagcGGGATTGCTCGTGCTCTTATTCTTGAACCGAAGCTGATGATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGNATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  2:142101/293‑1 (MQ=255)                                                                                                                                            aGCGTATCTGGATTGCGCGTGCTCTTATTCTTGAACCGAGGCTGATTATCTGGGATGAGCCGGGGGCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATGAATTTT‑ATCGCGCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGTTGAGGTATCTCGGCCAGGCGGTAGAACTGGGGACCTAGGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  1:521704/300‑1 (MQ=255)                                                                                                                                            aGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  1:31980/300‑1 (MQ=255)                                                                                                                                            aGCGTAGCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTTTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGGTGGGATTGTCATTAATTTT‑ATCGCTCAGGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGTTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATAcc                                                                                                                                                   <  1:307821/300‑1 (MQ=255)                                                                                                                                                               tGCTCTTATTCTGGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCGGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGagaag                                                                                                                                <  1:134021/300‑1 (MQ=255)                                                                                                                                                                 ctctTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGTCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGNGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAgaagaa                                                                                                                              <  2:550291/300‑1 (MQ=255)                                                                                                                                                                       ttCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAAc                                                                                                                        <  1:344627/300‑1 (MQ=255)                                                                                                                                                                                               gtgATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAAtt                                                                                                >  1:82635/1‑300 (MQ=255)                                                                                                                                                                                                                gcgcTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAAcc                                                                               >  1:159504/1‑300 (MQ=255)                                                                                                                                                                                                                 cgcTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAAccc                                                                              <  1:382461/300‑1 (MQ=255)                                                                                                                                                                                                                                    ggCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTc                                                           >  1:395048/1‑300 (MQ=255)                                                                                                                                                                                                                                        caGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATNTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTa                                                       >  1:445712/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggtggtCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGTGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGACGATCAACCCGCCTTCCGGTTGTGTTTTCCGTAcc                                                     >  2:104568/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggtggtCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTAcc                                                     >  2:487470/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGAtt                                         >  2:568932/1‑300 (MQ=255)                                                                                                                                                                                                                                                              aaTTGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTcc                                 <  1:72774/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCaga                              >  1:620629/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gCAACGTGAGATGGGATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCagag                             <  1:315175/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   cAACGTGAGGTGGGATTGTCATTAATTTG‑ATCGCTCATGACCGGGCCGGGGTAAAACACATTTCCGATTGTGTGTTGGTGATGTATCTCGGCCATGCGGGAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCNATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTNCGTACCCGTTGCCCGATTGCCGGTCCAGAGt                            <  2:445027/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            attggATTGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGGTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCaaaa                   <  2:169324/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                   tGTCATTAATTTT‑ATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTcc            <  2:295437/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         ttaattttATCGCTCATGACCTGGCCTTGGTAAAACACATTTCCGATCGTGTGTTGTTGATGTATCTCGGCCATGCGGTAGAACTGTGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTc       <  2:297257/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                            attttATCGCTCACGACCTGTCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTACCTCGTCCATGCGGTAGAACTGTGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTgg    <  2:25646/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tttATTGCTCATGACCTGGCCGTTGTAAAACACATTGCCGATCGTGTTTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGGCCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAGTTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAg  <  2:86421/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tttATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAg  <  1:228568/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tttATCGCTCATGACCTGGCCGTGGGAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCNACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAg  <  1:425735/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tttATCGCTCATGACCTGGCCGTGGGAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAg  <  1:647463/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                          GCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTCGGCGCTGGACGTGTCAATTCAGGCACAGGTGGTCAACCTGCTCCAGCAATTGCAACGTGAGATGGGATTGTCATTAATTTTTATCGCTCATGACCTGGCCGTGGTAAAACACATTTCCGATCGTGTGTTGGTGATGTATCTCGGCCATGCGGTAGAACTGGGGACCTATGATGAGGTCTACCACAATCCACTACATCCTTACACCAGGGCATTGATGTCGGCAGTCCCCATACCTGATCCGGATCTGGAGAAGAACAAAACCATCCAGTTACTGGAAGGGGAATTACCGTCGCCGATCAACCCGCCTTCCGGTTGTGTTTTCCGTACCCGTTGCCCGATTGCCGGTCCAGAGTGCGCCAAAACACGTCCTGTTCTGGAG  >  NC_000913/1306136‑1306720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑