Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,359,314	G→A	Q240* (CAG→TAG)	cadB ←	lysine:cadaverine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,359,314	0	G	A	97.7%	160.5 / NA	43	Q240* (CAG→TAG)	cadB	lysine:cadaverine antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/21);  minor base C (1/0);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTTCGGAATACCGTTGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACC  >  NC_000913/4359070‑4359558                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     tttttCGGAATACCGTTGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTc                                                                                                                                                                                                                                                  <  2:439774/249‑1 (MQ=255)    ttCGGAATACCGTTGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAg                                                                                                                                                                                                                                              <  1:304378/250‑1 (MQ=255)            aCCGTTGCTATCGACTTCACCACAAACTTTCGTGAAGTTACCGTCGTTAGCGGCACGTACACCTACCGGGCCTACCAACATCCTCCAGGGGCACAGAGAAGTCAGGCCCGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCGTCCCAGTAACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCTGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCTCAGCGATGTaaa                                                                                                                                                                                                                                     <  2:30261/251‑1 (MQ=255)               gTTGCTGTAGAATTCACCATAAACTGTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATAATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACa                                                                                                                                                                                                                                   >  2:181200/1‑250 (MQ=255)                 tGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAAt                                                                                                                                                                                                                                 >  1:17130/1‑250 (MQ=255)                 tGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAAt                                                                                                                                                                                                                                 >  2:15114/1‑250 (MQ=255)                    tGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCt                                                                                                                                                                                                                             >  2:433585/1‑251 (MQ=255)                                cATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATAACCAAGGAGCCAAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTAAAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACATGCTAAACCAGt                                                                                                                                                                                                                  >  1:419115/1‑250 (MQ=255)                                   aaaCTTTCGGGAAGTTACCGTCGTTATCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTACACAATACCTGCTAAACCAGTcccc                                                                                                                                                                                                              >  2:61529/1‑251 (MQ=255)                                    aaCTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTAccc                                                                                                                                                                                                              >  1:425155/1‑250 (MQ=255)                                           gggAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGt                                                                                                                                                                                                      >  2:125819/1‑251 (MQ=255)                                           gggAAGTTACCGTCGTTAGCGGCACGTACACCGGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTGGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGt                                                                                                                                                                                                      <  2:182937/251‑1 (MQ=255)                                                   aCCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGc                                                                                                                                                                                               <  1:15114/250‑1 (MQ=255)                                                        cgtTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACg                                                                                                                                                                                         <  1:210079/251‑1 (MQ=255)                                                        cgtTAGCGGCACGTACAACTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAGACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACg                                                                                                                                                                                         >  1:81146/1‑251 (MQ=255)                                                            aGCGGCACGTACACCTGCCTGGCCTACCAACATCACCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTa                                                                                                                                                                                      <  2:86695/250‑1 (MQ=255)                                                                            gcctgGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTaa                                                                                                                                                                      <  1:104664/250‑1 (MQ=255)                                                                                      caaacctaatcCCGGAGCCCAGAGAAGCCAGGCCTGCAAAGGCGGTGAAGGCAGAAACCAGCGGCCCAGCCCAGTTCCCGAGGATAGTTGAAGCACTGAGTGCAAACGGGGAACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCatta                                                                                                                                                           <  2:331146/243‑4 (MQ=255)                                                                                          aatcatcCAGGAGCCCAGATAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGTGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAATCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATATCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTGTTAACCATACCAGTACTTa                                                                                                                                                       <  2:261753/250‑1 (MQ=255)                                                                                           atcatcCGGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGGGAATGCAGAAACCAGCGGCGCAGCCCGGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCATTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTAc                                                                                                                                                      <  2:190558/251‑1 (MQ=255)                                                                                                ccAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTg                                                                                                                                                 >  1:213196/1‑251 (MQ=255)                                                                                                              aaGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACAccc                                                                                                                                   <  2:162549/251‑1 (MQ=255)                                                                                                                            aaGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAg                                                                                                                     <  2:159509/251‑1 (MQ=255)                                                                                                                                     aaTGCAGAACCCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGTTTGCAAACGGAGCCCCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGgcagagcag                                                                                                            <  2:17130/251‑1 (MQ=255)                                                                                                                                          aGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACAAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCCGAATAc                                                                                                       >  2:249720/1‑251 (MQ=255)                                                                                                                                                cAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGACGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTa                                                                                                  >  2:288039/1‑250 (MQ=255)                                                                                                                                                        cAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGc                                                                                          >  1:281018/1‑250 (MQ=255)                                                                                                                                                           cccAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGa                                                                                      <  1:209235/251‑1 (MQ=255)                                                                                                                                                                     gAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCcaca                                                                                                                      >  2:354199/1‑209 (MQ=255)                                                                                                                                                                     gAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCcaca                                                                                                                      <  1:354199/209‑1 (MQ=255)                                                                                                                                                                       ggATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGt                                                                           >  2:308105/1‑250 (MQ=255)                                                                                                                                                                         aTAGTTGA‑GCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATcc                                                                       <  1:357184/251‑1 (MQ=255)                                                                                                                                                                                        aTTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGtt                                                         >  1:4214/1‑251 (MQ=255)                                                                                                                                                                                                 ggAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATTACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATa                                                <  1:197604/251‑1 (MQ=255)                                                                                                                                                                                                      cccGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTg                                           <  1:48530/250‑1 (MQ=255)                                                                                                                                                                                                            aGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCAt                                     <  2:179936/251‑1 (MQ=255)                                                                                                                                                                                                                gccATTACAGAAGACGGATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCaaa                                 <  1:298942/251‑1 (MQ=255)                                                                                                                                                                                                                 ccATTACAGAAGAAGGATAAATAGCCGAAAGCACCTCAACCGCAGCGCTGTAAACAATACCTGCTAAACAAGTGCCCAGCATGGTTGCCAGCGGAACGTTACGTTTCGGGTTTTTAACCATACCAGTTCTTACAGCTGAGGATTCAACACCCACGAAGACCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGCGGTATCCGCAGGATTCCAGTTAGCTGCATAAGTTGCCACAtaacac                                >  2:312439/1‑246 (MQ=255)                                                                                                                                                                                                                                gATACATACCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGACGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCcaac                  >  1:55672/1‑250 (MQ=255)                                                                                                                                                                                                                                   acatacCGGAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAAt               >  1:323185/1‑250 (MQ=255)                                                                                                                                                                                                                                           gAAAGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGtcat      >  1:17761/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaGCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATCAGTTGCCGCATCAAACCAATGCCAGACAACACTAGCAGtcatc     >  1:39305/1‑250 (MQ=255)                                                                                                                                                                                                                                               gCACCTAAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGACCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATcacc  >  1:22696/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     TTTTTCGGAATACCGTTGCTGTCGACTTCACCATAAACTTTCGGGAAGTTACCGTCGTTAGCGGCACGTACACCTGCCTGGCCTACCAACATCATCCAGGAGCCCAGAGAAGTCAGGCACGCAAAGGCGGTGAATGCAGAAACCAGCGGCGCAGCCCAGTTACCGAGGATAGTTGAAGCACTGATTGCAAACGGAGCACCGGAAGCCGCCATTACAGAAGACGGATACATACCGGAAAGCACCTGAGTCGCAGCGATGTAAACAATACCTGCTAAACCAGTACCCAGCATGGTTGCCAGCGGAACGGTACGTTTCGGGTTTTTAACCATACCAGTACTTACAGCTGCGGATTCAACACCCACGAAGGCCCACAGGCAGAGCAGAATACTTTTAATGATCGCATGACCATCAGTGGTATCCGCAGTATTCCAGTTAGCTGCATAAGTTGCCGCATCAAACCAATGCCAGCCAACAATAGCAGTCATCACC  >  NC_000913/4359070‑4359558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑