Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,294	C→G	noncoding (35/85 nt)	tyrV ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,294	0	C	G	96.9%	102.3 / NA	32	noncoding (35/85 nt)	tyrV	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (12/19);  minor base A (1/0);  total (13/19)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAT  >  NC_000913/1287062‑1287538                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     gTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGcc                                                                                                                                                                                                                                    >  1:193359/1‑251 (MQ=255)   gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGGTTTAGAGTCTGCTCCCCTTGGCCgg                                                                                                                                                                                                                                  >  2:62703/1‑250 (MQ=255)   gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGc                                                                                                                                                                                                                                  >  1:365368/1‑251 (MQ=255)      ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCg                                                                                                                                                                                                                               <  1:153606/251‑1 (MQ=255)             ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAAccc                                                                                                                                                                                                                        >  2:104128/1‑251 (MQ=255)                           catcgctgcgcgctgcgCCCGGCGTGTCGTTGTCTGCGGCAACGCTCTCTCGCTCGCGCATGAGTCGAATCTTTGTCGGAGCGTCTCATCCTTCCTCGCATGGGCATAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAGCCTTCGAAGTCGATGACGGCAGAGTTAGAGTCGGCTCGCTTTGGCCGCTCGGGAACCCCACCAGGGGTAAtt                                                                                                                                                                                                          <  2:50088/248‑1 (MQ=21)                           cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAAtt                                                                                                                                                                                                          >  2:148828/1‑251 (MQ=255)                           cAGCGCTACGCGCTTCGCCATTCGGGTCGTTGTCTGCGGCAACGCTATCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCCGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGCAAGAGATGGTGGTGTGGGAAGGATTCGAACCTTCGAAGTCGATGCAGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCGCAGGAACCCAATCAGGGGTAAtt                                                                                                                                                                                                          >  2:130056/1‑251 (MQ=18)                            aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGGAAttt                                                                                                                                                                                                         >  2:366073/1‑250 (MQ=255)                                  gcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGTCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAAttt                                                                                                                                                                                                   <  2:300059/251‑1 (MQ=255)                                                           ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGCTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAGTTCAGATTTTGAGGTAATGCTTGCGAtgggggcg                                                                                                                                                                          >  2:392694/1‑246 (MQ=255)                                                                ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTggggg                                                                                                                                                                      >  1:32974/1‑250 (MQ=255)                                                                  cAACGCTCGCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAg                                                                                                                                                                   <  2:93108/251‑1 (MQ=255)                                                                      gCTCTCGCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGatt                                                                                                                                                               <  2:110018/251‑1 (MQ=255)                                                                                         ggTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGctcctc                                                                                                                                            <  2:257370/250‑1 (MQ=255)                                                                                         aGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGctcctc                                                                                                                                            <  2:32974/251‑1 (MQ=255)                                                                                                   tGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCg                                                                                                                                    <  1:148828/249‑1 (MQ=255)                                                                                                                 cATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCtgt                                                                                                                       <  2:171875/248‑1 (MQ=255)                                                                                                                             cATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCg                                                                                                        <  1:242876/251‑1 (MQ=255)                                                                                                                                  gCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTc                                                                                                   <  1:76880/251‑1 (MQ=255)                                                                                                                                      aaTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCGGTGGCAACGTTCTCTCGCTTTCGCtc                                                                                               >  1:333146/1‑251 (MQ=255)                                                                                                                                       atatTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGGAATTCAAATTTTGAGGTAATGCTTGAGATGGGGGTGGGGGAAGGATTATTCGTCGCTTCGCGCCTCACCATTGGGGCCGTTGCCTGAGGCTGCGTTCTCTCGCTttcgttcg                                                                                              >  1:107769/1‑251 (MQ=37)                                                                                                                                                           tGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAGTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTGGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAg                                                                           <  1:205069/250‑1 (MQ=255)                                                                                                                                                            gggAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAgg                                                                          <  1:87254/250‑1 (MQ=255)                                                                                                                                                               gaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTtct                                                                      <  1:329100/250‑1 (MQ=255)                                                                                                                                                                    cagtgACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCAcc                                                                  <  1:98083/246‑1 (MQ=255)                                                                                                                                                                       ggACTTTTGGGAGTGAGGGGGGTGGGGGACGGATCCGATCCGTCGAAGTCGATGACGGAAGATTTAGACTCTGCTCGCTTTGGCCGCGCGGGAAATCCGCCAGGGGTAATTCAAATTTTGAGGTAATGGTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCAAACCTTAGTCGAAGGTTCTCACCCTTc                                                              <  2:406544/251‑1 (MQ=255)                                                                                                                                                                                    tGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAAAGTCTGCTCCCTTTGGCCGATCGGGAACCCCACCAGGGGAAATTCCAATTTTGCGGTAATGCTTGAGCTGGTGGAGGGGGACGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCAGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTCCTCACCCTTCCCCGATGAGTGc                                                  >  1:453362/1‑250 (MQ=37)                                                                                                                                                                                      aTGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCaaa                                               <  1:402502/251‑1 (MQ=255)                                                                                                                                                                                              gggggAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGCAATTCAATTTTTCTGGCAATGCTTGAGATGGGGGAGGGGGACGGATTATTTGTCGCTTCGCTCCTCACTCTTCGGGCAGGTGCCGGAGGCAACGTTCTCTCGCTTACTCTCGCATCGAACGTTATTCGGAGGACCTGACCTTTCCCCTATGAGTGCAAAAtttaacc                                       >  2:393912/1‑247 (MQ=16)                                                                                                                                                                                                                         gATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCtgt            <  1:89716/251‑1 (MQ=255)                                                                                                                                                                                                                                   gATTTAGAGTCTGCGCCCTGTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTGTGAGGTAATGATTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAt  <  1:286983/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     GTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAT  >  NC_000913/1287062‑1287538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑