Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,108	G→A	intergenic (‑177/‑295)	caiT ← / → fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,108	0	G	A	100.0%	65.8 / NA	19	intergenic (‑177/‑295)	caiT/fixA	L‑carnitine:gamma‑butyrobetaine antiporter/putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

TGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTT  >  NC_000913/41862‑42321                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      tGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCg                                                                                                                                                                                                                   >  1:60564/1‑251 (MQ=255)    cgacgaTTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGttt                                                                                                                                                                                                                <  2:377631/251‑1 (MQ=255)      acgaTTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGttttt                                                                                                                                                                                                              >  2:437806/1‑251 (MQ=255)                          gaaaACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAAGTTGGATtaataa                                                                                                                                                                                          <  1:85769/251‑1 (MQ=255)                             aaCCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTGCGTTAATTTTGATTAATAATCa                                                                                                                                                                                       <  2:432375/251‑1 (MQ=255)                                  tCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGttt                                                                                                                                                                                   <  2:150093/250‑1 (MQ=255)                                       tCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTAt                                                                                                                                                                              <  2:80237/250‑1 (MQ=255)                                            tCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGctct                                                                                                                                                                         <  2:109331/250‑1 (MQ=255)                                                           ttCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGATGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAAt                                                                                                                                                         >  2:262382/1‑251 (MQ=255)                                                                   tcATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCt                                                                                                                                                  <  1:424835/250‑1 (MQ=255)                                                                            aaTTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCaa                                                                                                                                         <  2:42767/250‑1 (MQ=255)                                                                                                    tatttTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAatat                                                                                                                 <  1:296508/250‑1 (MQ=255)                                                                                                              tCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCt                                                                                                      >  1:19162/1‑251 (MQ=255)                                                                                                                 cGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCtttt                                                                                                   >  1:269872/1‑251 (MQ=255)                                                                                                                   tttgtatTATATTTGTTAACATTTACTATAATTCTTATTAAACTCGTGGAAGCTTTAATGGCTTAATCATAATGGGTATTCACTACCCTGTCTTCTGAGATTGGTATCACATTTTTGTTTCGGGTGAATAGAATGCGTTTTTTCGTTAATTTTGCTTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTTATCCATAAAACAATATTGAAAATTTCtttttt                                                                                                 >  2:66396/1‑250 (MQ=255)                                                                                                                      gtttTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACGCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCGGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTa                                                                                              <  1:262382/251‑1 (MQ=255)                                                                                                                               ttGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGtt                                                                                     >  2:183154/1‑251 (MQ=255)                                                                                                                                                                gTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGa                                                     >  2:201233/1‑250 (MQ=255)                                                                                                                                                                                                                   gtgATTGGTATCACATTTTTGTTTCGGGTGAATAGAAGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGtttt  <  1:28636/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      TGCCGACGATTATTAACGGCGGAAAGAAAACCTTCGGTTCTATTCCCGTTTTTCTCTTTTCATTCTTCATGAGTTAATTCCACTGTGAAAACGAATATTTATTTTGCGTTCCCGTTTGTTTTATTTTTGTTAACATTTAATATAATTATTATTAACCTCGTGGACGCGTTAATGGCTAACTCATAATGGGTATTCAATAAGCTGTATTCTGTGATTGGTATCACATTTTTGTTTCGGGTGAATAGAGGGCGTTTTTTCGTTAATTTTGATTAATAATCAGTTTGTTATGCTCTGTTGTGAGTAAAAAATAACATCTGACTTTCAATATTGGTGATCCATAAAACAATATTGAAAATTTCTTTTTGCTACGCCGTGTTTTCAATATTGGTGAGGAACTTAACAATATTGAAAGTTGGATTTATCTGCGTGTGACATTTTCAATATTGGTGATTAAAGTTTT  >  NC_000913/41862‑42321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑