Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,786,5360AG28.8% 141.8 / 12.1 67E426G (GAA→GGA) gpmM2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base A (7/40);  new base G (19/0);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 3.80e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCC  >  NC_000913/3786292‑3786785
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           
aaCGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCACCTCACAGACAGAGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGAACGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTCGGTCCCACCGGGGTGATGGAAGCGGCGGGTAAAGCGGGGGGAGCGCTGGGTCACAGCGTGG‑gag‑aagg                                                                                                                                                                                                                                                      >  2:421953/1‑250 (MQ=255)
    gTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTcgcg                                                                                                                                                                                                                                                  <  1:248685/251‑1 (MQ=255)
      ggCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCCCCGAAAGAGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGGTCACTGCGTGG‑GAG‑AAGGCGCGa                                                                                                                                                                                                                                                 >  1:68435/1‑250 (MQ=255)
            gaagaGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGt                                                                                                                                                                                                                                          <  2:95577/251‑1 (MQ=255)
                agTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGATCACTGCGTGG‑GAGCAAGT‑GCGAAAgagggttgg                                                                                                                                                                                                                                      >  1:296983/1‑243 (MQ=255)
                      tCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCgt                                                                                                                                                                                                                                 <  1:29620/250‑1 (MQ=255)
                             cGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAACCGGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCTGTTAAAGCTGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGAc                                                                                                                                                                                                                         <  1:8202/251‑1 (MQ=255)
                                    cGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTTGATCCCTGCGTGG‑GAG‑AAGTCGCGCAAACGGGTGAGGCCgtgggggggccacggct                                                                                                                                                                                                                  >  1:2895/1‑239 (MQ=255)
                                     gCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGATGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAAAGGTTGAAGCGGTGGAGTACTGCGTGG‑GAG‑AAGACGCGGAAGCGGGTGAGGACGGGGGTGGACAAatgctg                                                                                                                                                                                                                 >  1:182424/1‑251 (MQ=255)
                                          cTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGATCACATCGTGG‑GAG‑AAGACGCGAAAACGGTTGAGTCCCTGGGGGGGCAAATGCTGATCAc                                                                                                                                                                                                            >  1:238682/1‑251 (MQ=255)
                                           tGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGACAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGCACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTTAAGCCGATGAAGCGGTGGGTAACTGCGGGG‑AAA‑GAGCCCCGCAAGCGGGTGAGGCCGTGGGGGGGCAActggcggtcacc                                                                                                                                                                                                           >  1:233845/1‑242 (MQ=255)
                                            gATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCAcc                                                                                                                                                                                                           <  2:434559/250‑1 (MQ=255)
                                            gATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCg                                                                                                                                                                                                          <  2:107571/251‑1 (MQ=255)
                                             aTCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGAGCACTGCGGGG‑GAG‑AAGGCGCGAAAGCGGGTGAGTCCGTGGGTGGGCAAACGATGATCACCGc                                                                                                                                                                                                         >  1:315492/1‑251 (MQ=255)
                                                  cTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGAGGTTGAAGGGCTGGGTCACTGCGTGG‑GAG‑AAGTCGCGAAAGAGGGTGAGTCCCTGGGGGGGCAACCGCTGGTAACCCCTGAcc                                                                                                                                                                                                    >  1:357194/1‑251 (MQ=255)
                                                      ccGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTg                                                                                                                                                                                                       <  1:129993/244‑1 (MQ=255)
                                                            gTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGCGCTGACCGCAAAACTGGTTGCGGCCATCAAGCGCGGCAAATACGACACCATCCTCTGTAACTCTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTTAAGCGGCTGACGCGCCGGGACCCTGCGCGG‑GAG‑AAGGCGCCAAAGCGGTTGAGTCCGTTGGGGGGCCACTGCTCATCCCCCCTGACCACGGTAACGc                                                                                                                                                                                          >  1:272777/1‑251 (MQ=255)
                                                               gCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGa                                                                                                                                                                                       <  1:275309/251‑1 (MQ=255)
                                                                  acctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCa                                                                                                                                                                                    <  1:147574/251‑1 (MQ=255)
                                                                              cAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGGAGCGCGGGGTAACTGCGGGG‑GAG‑AAGGCGCGAAAGCGGTTGAGGCCGTGGGGGGGAAAATGCTGAGCACGCCGGACCCCGGTAAAGCTGAGCAGATGGGCGGTcc                                                                                                                                                                        >  2:239224/1‑251 (MQ=255)
                                                                              cAACCGGAAAGGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATcc                                                                                                                                                                        <  2:69009/251‑1 (MQ=255)
                                                                                 cctgAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCTGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCTAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGc                                                                                                                                                                     <  1:438167/248‑1 (MQ=255)
                                                                                      aaTGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGGGGACAACTGCTGATCACCGCGGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACgg                                                                                                                                                                <  2:272777/251‑1 (MQ=255)
                                                                                          aGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCa                                                                                                                                                            <  1:269693/251‑1 (MQ=255)
                                                                                                  agagCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGGTCACTGCGGGG‑GAG‑AAGGCGCGAAAACGGTTGAGGCCGGGGGGGGGCAACTGCTGATCACCGCTGACCACCGGAAAGCTGAGCGGAGGCGCGATTCGGCCACGGGGCCggcacac                                                                                                                                                     >  2:66531/1‑250 (MQ=255)
                                                                                                   gagCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAggcacacac                                                                                                                                                   <  2:197054/251‑1 (MQ=255)
                                                                                                        gACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGGGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGGTGCGCGATCCGGCAACGGGTCAggcacacacggc                                                                                                                                                <  1:434423/249‑1 (MQ=255)
                                                                                                             aaaaaCTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTTCGTGG‑GAG‑AAGGCGCGAAAGCGGGTGAGTCCGGGGGGGGACAACTGCTGATCACCGCTGACCCCGGGAACGCTGAGCAGATGCGCGGTCCGGCCACGGGGCCGGCACCCACGGCCCACA‑cc                                                                                                                                        >  2:110999/1‑251 (MQ=255)
                                                                                                              aaaaCTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCa                                                                                                                                       <  2:233845/251‑1 (MQ=255)
                                                                                                               aaaCTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTGGGAGCGCTGGGTCACTGCGTGGGAGG‑AAG‑CGCGAAAACGGGTGAGGCCGGGGGGGGGGAACTGCTGATCACCCCTGACCCCGGGAAAGCCGTGCAGGTGCGCGGTCCGGCCACGGGGCAGGGACACCCCGcaccca‑cccc                                                                                                                                      >  2:271469/1‑249 (MQ=255)
                                                                                                                     gTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGGGCTGGGTCACTGCGGGG‑GAG‑AAGGCGCGAAAGCGGGTGGATCCGGGGGGGGACAAATGCTGATCACCCCCGGCCACGGGAACGCTGGGCAGAAGCGGCATACGGCAACGGGGGCGGCACACAAGGCCCAcaaccaacatcc                                                                                                                                 >  2:355681/1‑247 (MQ=255)
                                                                                                                           gCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTcc                                                                                                                          <  1:452732/251‑1 (MQ=255)
                                                                                                                            ccATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAGAGGGTGAAGCGCTGGATCACAGCGGGG‑GAG‑AAGGGGCGCAAGCGGGTGGGGCCGGGGGGGGGCAAATGCTGATAACCCCCGACCCAGGGAACGCGGAGCCGAAGCGCGATCCGGCCACGGGGCAGGCACACACGGGACACC‑CAAACCTGCCAGTTccc                                                                                                                         >  2:58109/1‑250 (MQ=255)
                                                                                                                                cAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTg                                                                                                                      <  1:327076/250‑1 (MQ=255)
                                                                                                                                cAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAACGGTTGAAGCGCGGGAACAACGCGAGG‑GAG‑AAGGCGCGGAAGCGGTTGAGTCCGCGGGGGGGGAACTGCTGATTCCCGCTGACCAACGTAACGGCGCGCCGATGCGCGATCCGGCAACGGGGCCGGCACACACGGCACACA‑CCCACCTGCCAGTTCCGCTgg                                                                                                                     >  1:466622/1‑250 (MQ=255)
                                                                                                                                 aaGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGAt                                                                                                                    <  1:81471/251‑1 (MQ=255)
                                                                                                                                 aaGAGCGGCAAATACGACACCATCATCTGTAACAATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGAt                                                                                                                    <  1:107575/251‑1 (MQ=255)
                                                                                                                                   gagCGGCAAATACGACACCATCATCAGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGAttt                                                                                                                  <  1:387799/251‑1 (MQ=255)
                                                                                                                                   gagCGGCAAATACGAAACGAACATCTGGAACTAGCCGAACGGCGACATGGGAGGTCACACAGGGGGGATGGAAGCGGCGGTTAAAGCGGGGGAAGCGCTGGTTCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAGCTGCTGATCACCGCGGACCACGGTAACGCTGAGCATATGCGCGATCCGGCATCGGGTCAAGCATACACGGTACACG‑CCAACCTGGCAGGTGCGCGGAttt                                                                                                                  <  2:460163/251‑1 (MQ=255)
                                                                                                                                     gCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTAc                                                                                                                <  2:106688/251‑1 (MQ=255)
                                                                                                                                          aaaTACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAACGGTTGGAGCGCTGGGTCACTGCGTGG‑GAG‑AAGGCGCGAAAGAGGGTGAGTCCGTGGGGGGGCAACTGCCGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGCACCGGCAAAGGGGCCGGCACACACGGCACACA‑CCCACCTGCCCGGTCCGCTGAttttcagtgg                                                                                                           >  2:69156/1‑244 (MQ=255)
                                                                                                                                            aTACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGCTAAAGCCGTTGAAGCGCAGGATCACTGCGTGG‑GAG‑AAGTCGCGAAAGCGGGTGAGTCCGTGGGGGGGCAACTGCTGATCACCGCTGACCCCGGGAAAGCCGAGCAGATGCGCGATCCGGCAACGGGGCAGGCACACACGGCACACA‑CCAAACTGCCAGGTCCACTGAttttcggtgggg                                                                                                         >  1:422791/1‑242 (MQ=255)
                                                                                                                                              ccGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGAt                                                                                                       <  2:72772/250‑1 (MQ=255)
                                                                                                                                                gACACCATCATCTGTACCTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGAGGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATaa                                                                                                     <  2:2895/251‑1 (MQ=255)
                                                                                                                                                     catcatCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGAGGGTGGAAAGCTGGGTCACTGCGTGG‑AAG‑AAGTCGCGGAAACGGGTGAGTCCGGGGGGGGGCAAATGCTGATCACCGCTGACCACGGGAACGCCGGGGAGAGTCGCGATCCGGCAACGGGGCCGGCACACAAGGCACACA‑CCAACCCGCCCGGTCCGCTGATTTACGTTGGTGGTAAGaaac                                                                                                >  1:170117/1‑249 (MQ=255)
                                                                                                                                                             gTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAg                                                                                          <  1:368254/249‑1 (MQ=255)
                                                                                                                                                               aaCTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGGAAGCGGTTTAGGCCGTGGGGGGGCAACTGCTGGTCACCCCTGGCCACGGGAACGCTGGGCAGATGCGCGATTCGGCCACGGGTCAGGGACACACGGCACACA‑CCAACCTGCCCGTTCCGCTGATTTTCGTTGGTGATAAGAACGTGAAAGCGGt                                                                                      >  1:324235/1‑251 (MQ=255)
                                                                                                                                                                 cTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGGGG‑CAG‑AAGTCGCGAAAGCGGGTGAGTCCGGGGGGGGACAAATGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGGCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGGTAAGAACGTGAAAGCgggtg                                                                                    >  1:39940/1‑248 (MQ=255)
                                                                                                                                                                   aTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGAACACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGaa                                                                                  <  2:315492/251‑1 (MQ=255)
                                                                                                                                                                            gCGAAATGGTAGGTCACACCGGGGTGAGGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCaaa                                                                         <  2:182424/251‑1 (MQ=255)
                                                                                                                                                                             cGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGGTCACTGCGGGG‑GAG‑AAGGCGCGAAAGCGGGTGAGTCCGTGGGGGGACCACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAAAGGGGCAGGCCCAAACGGCACCCA‑CCAACCCGCCCGTTCCGCTGATTTACGCTGGTGATAAGAACGGGAAAGCGGGTGAAGGCGGCaaa                                                                         >  2:359206/1‑250 (MQ=255)
                                                                                                                                                                                 aTGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑GAG‑AAGTCGCGAAAGCGGTTTAGTCCGTGGGGGGGCAACTGCTGGTCACCGCCGACCACGGGAACGCTGAGCAGATGCGCGATCCGGCAACGGGGCAGGCACCCACGGCACACA‑CCAACCCGCCAGTTCCCCTGCTTTTCGTTGGGGGTAAGAACGGGAAAGCGGTTGAAGGAGGCAAACTTTc                                                                    >  1:395561/1‑251 (MQ=255)
                                                                                                                                                                                  gggTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCt                                                                   <  2:415296/250‑1 (MQ=255)
                                                                                                                                                                                   ggTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTg                                                                  <  1:372115/251‑1 (MQ=255)
                                                                                                                                                                                     tAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAACGGTTGAAGCGCTGGGTCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGGTGAGTCCGTGGGGGGACAACTGCTGGTCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCCACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGGTGAAGGCGGCAAACTTTCTGAc                                                                >  1:300308/1‑251 (MQ=255)
                                                                                                                                                                                      aGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGTTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCGGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCTCACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGAGAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACa                                                               <  1:453911/251‑1 (MQ=255)
                                                                                                                                                                                        gTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGGTCACTGCGTGG‑GAG‑AAGTCGCGAAAGAGGGTGAGTCCGTGGGGGGACAACTGCTGATCACCGCCGACCCCGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCCAACC‑CCAACCTGCCAGTTCCCCTGATTTACGTTGGTGATAAGAACGCGAAAACGGGTGAAGGCGGCAAAATTTCTGACATc                                                             >  1:81820/1‑251 (MQ=255)
                                                                                                                                                                                           ccacCAGGGTGAGGGAAGCGGCGGTTAAAGCGGTTGACGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATcgcg                                                          <  2:347742/250‑1 (MQ=255)
                                                                                                                                                                                                     gATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCAtg                                                 <  1:293835/250‑1 (MQ=255)
                                                                                                                                                                                                          aaGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCg                                           <  1:359206/251‑1 (MQ=255)
                                                                                                                                                                                                              ggcggTTAAAGCGGTTGAAGCGCTGGAGCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGa                                       <  2:70383/251‑1 (MQ=255)
                                                                                                                                                                                                                cggTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCGGACATCGCGCCGACCATGTTGTCGCTGATg                                     <  1:176122/251‑1 (MQ=255)
                                                                                                                                                                                                                 ggTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACTTCGCGCCGACCATGTTGTCGCTGATgg                                    >  2:370644/1‑251 (MQ=255)
                                                                                                                                                                                                                    tAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCCCGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGt                                  <  2:177724/250‑1 (MQ=255)
                                                                                                                                                                                                                              gAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTGGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATcc                        <  1:365342/250‑1 (MQ=255)
                                                                                                                                                                                                                                  cgcTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGAACGAGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGAAAAGGGTCAAGCAAACACGGAACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTATGACATCGCGCCGACAATGTTGTCGATCATGGGAATGGAACTCACGCa                    >  1:427352/1‑250 (MQ=255)
                                                                                                                                                                                                                                       gATCACTGCGTGG‑AAG‑AAGTCTCGAAAGCGGTTGAGGCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGAt              <  2:128725/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGt       <  1:316945/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   g‑aag‑aaGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGcc  <  1:150566/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaag‑aagTCGCTAAAGCGGTTGTGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGTTAACGCTGAGCAGATGCGCGATCCTGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTTCGTTGGTGATAAGAACGTGAAAGCTGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATTTTGGCGCTGATGTGGATGGAAATCCCGCAAGAGATGACTGGTAAGcc  <  1:278355/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                           
AACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGG‑AAG‑AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA‑CCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCC  >  NC_000913/3786292‑3786785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑