Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,718	0	G	C	32.6%	72.6 / 15.6	45	P292R (CCG→CGG)	yqeF	putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (3/26);  new base C (14/0);  total (18/27)
Fisher's exact test for biased strand distribution p-value = 8.68e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATC  >  NC_000913/2984478‑2984953                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ttGCGGGCATTACGTGTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCGCTCAATCATCTTGCCAACCTTAAGCGCCTGTGCAGCAAACGTTTCATTAGCCTCGATAAGATCTACTTCTGCCAACTGCGAGCCTACGCGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAAtt                                                                                                                                                                                                                                              <  1:304722/249‑1 (MQ=255)           tccGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCACCCGGAAGCGCCTATCGGGGGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCGCTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCGCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACGCCG‑GCGCGATTC‑CCATC‑aacgc                                                                                                                                                                                                                                 <  2:307126/248‑4 (MQ=255)                    ccATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGAGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCGTACAACC‑GCGCaaataccaccacagaccggtcgaa                                                                                                                                                                                                                          >  2:248481/1‑228 (MQ=255)                         tCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACACCC‑GCCCAATTC‑CCCAC‑AATA‑CCG‑GATCTca‑accaac                                                                                                                                                                                                               >  1:404030/1‑251 (MQ=255)                             gaaacAGAGAAACCAGGATTCGGCCACCGGAAGCGCCTATCGGGTGACCCAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCATCCTAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGCCTTCAGCCAACTGCCATCCTACACGCTCCAGGCAACGGCGGGTCGCATACATCT‑GTGCAATTC‑CCATC‑AATC‑CCG‑GATCTAC‑ACCAACGCt                                                                                                                                                                                                            <  2:279486/246‑1 (MQ=255)                                           aGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AAt                                                                                                                                                                                           <  1:338166/251‑1 (MQ=255)                                                    cAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACCCCC‑GCGCCATTC‑CCATC‑CATG‑CCG‑GATCTAC‑ACCCACCCTCGC‑AAAATCGCG‑AACGCGGGcccg                                                                                                                                                                                  >  1:205601/1‑249 (MQ=255)                                                       ccGGAAGCGCCTCTCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCAAACCCCC‑GCGGAAATT‑CCATA‑AAAG‑GCG‑GGTCTAC‑ACCAAAGCATGC‑AAATGCGCG‑ACAGCGGGCCAGCa                                                                                                                                                                                >  1:92195/1‑250 (MQ=255)                                                          gAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACggg                                                                                                                                                                            <  1:451546/251‑1 (MQ=255)                                                                ccTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑GCATC‑AATG‑CGG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAAtt                                                                                                                                                                     <  2:59364/251‑1 (MQ=255)                                                                     cGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCC‑GCGCAATTC‑CCCTC‑CATGGCCG‑G‑TCTAC‑ACCAACGCTTGC‑AAATGCGCG‑AAAGCGGGCCCGCACGGG‑GAAATTccaac                                                                                                                                                                >  1:162811/1‑247 (MQ=255)                                                                                gTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTg                                                                                                                                                     <  1:60163/251‑1 (MQ=255)                                                                                         cgcCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACACCC‑GCGCAATTC‑CCCCC‑CAAG‑CCG‑GGTCTAC‑ACCAACGCTCGC‑AAAAGCGCG‑CATGCGGGCCAGCACGGGGAAAATTCAAGCCCCGTGCTTTgcctctctc                                                                                                                                             >  1:197024/1‑243 (MQ=255)                                                                                         cgcCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCtc                                                                                                                                            <  1:132241/251‑1 (MQ=255)                                                                                          gcCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACCCCC‑GCCCAATTC‑CCCTC‑AATT‑CCC‑GGTCTAC‑CCCAACCCCGGC‑ACATGCGCG‑ACTGCGGGCCACCACCGG‑TAAAATCAACCCCCCTTCTTTTGCTTCGCtcc                                                                                                                                           >  2:285817/1‑250 (MQ=255)                                                                                           ccaccaTTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCg                                                                                                                                                                                        <  1:101863/206‑1 (MQ=255)                                                                                                      attgacCCGACGCTCATCCCACTCAAGCATCTTGCCGACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTTCTTTGGCTTCGCTCATCATCATTACCg                                                                                                                               <  1:363981/251‑1 (MQ=255)                                                                                                        tgacCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTAAACCG‑GCGGAATTC‑CCATC‑CATT‑CCG‑GGTCTAC‑ACCAAAACTGGC‑AAATGAGCG‑AATGAGGGACAGCAAGGG‑AAAAATCAAACCTCGTGCCTTGGGTTCACtcatccatattaaccg                                                                                                                              >  1:6534/1‑239 (MQ=255)                                                                                                           cccGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCt                                                                                                                          <  2:140027/251‑1 (MQ=255)                                                                                                                    cATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCAt                                                                                                                   <  1:128611/249‑1 (MQ=255)                                                                                                                     aTCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCATCTGCGCCATCG‑t                                                                                                               <  1:102256/251‑1 (MQ=255)                                                                                                                             cAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATgg                                                                                                         <  1:443813/249‑1 (MQ=255)                                                                                                                                           aCCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCCCAATTC‑CCATC‑AAAG‑CCG‑GGTCTAC‑ACCAACCCTGGC‑CAATGCGCG‑AAAGCGGGCCCGCACGGG‑GAAATTCAACGCCCGCGCTTTTGGTTCTCTCCTTATCATTACCCCAGCTGCGCCCTCG‑TTTAttggggagtcatttccccc                                                                                         >  1:86861/1‑233 (MQ=255)                                                                                                                                             cGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCC‑GCGCAATTC‑CCCTC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCCGCACGGG‑GAAATTCAAAGCTCGTGCTTTGGCTTTGCTCATCATCATTACCGCCGCTGCGCCCTCC‑TTTATgggtgcttcattaacccccg                                                                                       >  1:266512/1‑233 (MQ=255)                                                                                                                                                 aGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCAGC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTcac                                                                                   <  2:127187/251‑1 (MQ=255)                                                                                                                                                    gcCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGa                                                                                <  2:266512/251‑1 (MQ=255)                                                                                                                                                    gcCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCA‑GCGCAATTT‑CCCTC‑AATG‑CCG‑GATCTAA‑ACCAACGCTGGC‑AAATGAGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACACTCCTGCTTTGGCTTCGCTCATCATCATTACCGCCGCTGCGCCCTCG‑TTTATGGATAATGCATTTACCGCCGTcac                                                                                   >  1:463507/1‑248 (MQ=255)                                                                                                                                                      cTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTAGCAT‑CACCCCGCGCAAATC‑CCCTC‑AATG‑CCG‑GGTCTAC‑ACCAACGCTGGCGAAATG‑GCG‑AATGCGGGCCAGCACGGG‑TAAAATTAACGCTCGCGCTTTTGCTTCGCTCATCCTCATTTCAGCAGCTTCGCCATCG‑CTTTTGGATGATGCATTTCCCGCTggtaccagac                                                                              >  1:343182/1‑242 (MQ=255)                                                                                                                                                        gtgCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAAcc                                                                             <  1:334431/250‑1 (MQ=255)                                                                                                                                                          gcagcaAACGCTTCATTATCCGCGATAAGAGCGACTTCAGCCATCTGCCAGCCTACACGCTCCAGGCAACGTCGGGTCGCATTTTCTG‑TAATGATTC‑GCCGC‑CCT‑‑CCGAGATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCgag                                                                          <  2:355106/251‑1 (MQ=255)                                                                                                                                                              caAACGCTTCATTAGCCTCGATAATATCTACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGTTCGCATACCCCG‑GCGCAATGA‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTa                                                                      <  1:303536/251‑1 (MQ=255)                                                                                                                                                              caAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCACACCCCC‑GCGCCATTC‑CCCTCCAAT‑‑CCC‑GATCTCC‑ACCCACCCCGGC‑AAAAGCGCGGAAGCCGGGCC‑CCACGGGGAAAATTAAAC‑CCCGGGCTTTGGCTTCGCCCCCCATCATTTCCGCCGCCGCGCCCTCG‑TTTATGGGTTATGCATTAcccccctgcccacaaacccgact                                                                       >  2:46478/1‑230 (MQ=255)                                                                                                                                                               aaaCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTAt                                                                     <  1:277384/251‑1 (MQ=255)                                                                                                                                                                aaCGCTTCATTAGCCTCGATAAGATCGACTTCAGACAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACTCCG‑GAACAATTCGCCGCC‑CA‑G‑CGA‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATc                                                                    <  2:155493/251‑1 (MQ=255)                                                                                                                                                                   gCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACACCC‑GCGCCATTC‑CCCTC‑ACAG‑CCC‑GATCTAC‑CCCCACCCTGGC‑AAATGCGCG‑AATGCGGGGCAGCACGGG‑GAAAATCAACACCCGCGCTTTGGCTTCGCTCCTCATCATTATCCCCGCTGCGCCCCTG‑TTTTTGGTTGATGCATTAccccccgtccccgaacccagaacatcca                                                                  >  2:346078/1‑225 (MQ=255)                                                                                                                                                                     ttCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCCCATACACCC‑GGGCCATTT‑CCCTC‑AATG‑CCG‑GATCTAC‑ACCCACGCTGGC‑AAATGCGCG‑AATGCGGGGCAGCACGGG‑GAAATTCAACGCTCGTGCTTTGGCTTCGCCCATCATCATTACCGCCGCCGCGCCCTCG‑TTTATGGATGATGCATTACCCCCTGGCACCGAACCGAGACTATCAAAt                                                                >  1:273399/1‑250 (MQ=255)                                                                                                                                                                       cATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCTCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACTCTCGGGCTTTGGCTTCTCTCATCGTCATGTCCGGAGCTGCGCCATGG‑TTTATGTATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGa                                                              <  1:275256/250‑1 (MQ=255)                                                                                                                                                                           aGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGa                                                          <  1:98348/250‑1 (MQ=255)                                                                                                                                                                                         cGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACCCCC‑GCGCAAATC‑CCATC‑AATG‑CCG‑GGTCTACCACCA‑CCCCGGG‑AACAGCGCG‑CAAGCGGGGCCGCACGGG‑GAAATTTAACGCCCGTGCTTTTGCTTCTCTCCTCCTCATTACCCCACCTGCGCCCTCCCTTTTTGG‑TGGTGCATTTCCCCCCGTCACCGAAACCAGAATTTCAAATAAAGGATTTAAACGGGcgaac                                           >  2:282039/1‑247 (MQ=255)                                                                                                                                                                                              tCAGCCAACTGCCAGCCTGCACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCt                                        <  1:435795/249‑1 (MQ=255)                                                                                                                                                                                               cAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCt                                     <  1:342670/251‑1 (MQ=255)                                                                                                                                                                                                             ccTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCACAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGt                       <  2:106752/251‑1 (MQ=255)                                                                                                                                                                                                             ccTACACGCTCCAGGCAACGGCGGGTCGCATACACCC‑GCGCAAATC‑CCATCCAAT‑‑CCG‑GGTCTAC‑ACCAACGCCGGC‑AAATGCGCG‑AAAGCGGGCCAGCACCGG‑GAAATTCAACGCTCGTGCTTTTGCTTCGCTCATCATCATTACCACAGCTGCGCCCTCG‑TTTTTTGATGATGCATTACCCCCTGTCACAGAAACGCGACTATCAAATGAAGGATTTAAACGGGCTAAACCTTTTTCGCTGGCggccgt                       >  1:106752/1‑246 (MQ=255)                                                                                                                                                                                                                         aGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTc           <  1:283421/251‑1 (MQ=255)                                                                                                                                                                                                                                   gggTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATc  >  1:146085/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCG‑GCGCAATTC‑CCATC‑AATG‑CCG‑GATCTAC‑ACCAACGCTGGC‑AAATGCGCG‑AATGCGGGCCAGCACGGG‑TAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCG‑TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATC  >  NC_000913/2984478‑2984953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑