Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,450	0	T	G	37.2%	82.2 / 11.9	52	G114G (GGT→GGG)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (4/28);  new base G (19/0);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.83e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTA  >  NC_000913/2326204‑2326697                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                              aGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑gg                                                                                                                                                                                                                                                         <  1:402105/251‑1 (MQ=255)    aaTTAAAGCCGGCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGGGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGTCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGAGTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCTGGGGG‑TA‑T‑GGaa                                                                                                                                                                                                                                                       <  2:293167/250‑1 (MQ=255)             cgccATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGtt                                                                                                                                                                                                                                             <  1:33838/251‑1 (MQ=255)                            aaaaaTCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑G‑GGGAAATATGAGGTTAGCCCCCcactttc                                                                                                                                                                                                                              >  2:376547/1‑249 (MQ=255)                               aaTCGATTCACAACACGTTGCTGACGAGCTTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGAGCGTCAGGAACTGTTAAAACGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTA‑‑‑GGGAAATATGAGTTTAGccccccacctacccc                                                                                                                                                                                                                           >  2:49851/1‑241 (MQ=255)                                   gATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGGT‑GGAAAA‑ATGAGGTTGGCCCCCcacttatttgactg                                                                                                                                                                                                                       >  2:97773/1‑243 (MQ=255)                                   gATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GG‑T‑GGGAAATATGAGGTTAAGCCCCTACTTTCTCGaagg                                                                                                                                                                                                                       >  2:406078/1‑248 (MQ=255)                                              cGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GG‑T‑GGGAAATATGAGTTTTGCCCCCtcccttctccctgcaaaagaacgcc                                                                                                                                                                                                            >  2:219105/1‑227 (MQ=255)                                               gTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGGAAATATGGGGTTAGCCCCCTCCTTACTCGATGCAAAAG‑CAAGctc                                                                                                                                                                                                          >  1:183407/1‑251 (MQ=255)                                                ttGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑A‑GGTAAATATGAGGTTAGGCCCCCACTTACTCGAGTCAAAAG‑CACGctcc                                                                                                                                                                                                         >  2:54638/1‑250 (MQ=255)                                                  gaggaAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTg                                                                                                                                                                                                        <  2:194577/247‑1 (MQ=255)                                                        gTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGGAAAAATGAGGTTATCCCCCCACTTACTCGATGCAAAAG‑AACACTCTGGGTAtat                                                                                                                                                                                                 >  1:369811/1‑249 (MQ=255)                                                           aTTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCt                                                                                                                                                                                              <  1:70954/251‑1 (MQ=255)                                                           aTTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGGAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAA‑CACGCTCTGGTTATCGTCt                                                                                                                                                                                              >  1:249462/1‑251 (MQ=255)                                                             tATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCtt                                                                                                                                                                                             <  1:228540/250‑1 (MQ=255)                                                              aTGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCGTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTg                                                                                                                                                                                            <  1:279012/250‑1 (MQ=255)                                                                        tgtTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GG‑T‑GGGAAATATTAGGTTAGCCCCCCACTTACTCGATGCCAAAG‑CACGCCCTGGTTTTTGTCTTTGGGACgggccg                                                                                                                                                                                 >  2:140797/1‑247 (MQ=255)                                                                              aaGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑TGGAAATATGAGTTTAGGCCCCTAATTAATTGATGCAAAAG‑CACGCCCCGGTTATACTCTTGGGGACGGACAG‑GTTTAt                                                                                                                                                                          >  2:200938/1‑251 (MQ=255)                                                                                  cGGGCTGGGGCAAAATCCGGCGCGTCAGTCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCTGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGATCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTGATCGTCTTGGAGACGGACAG‑GTTTATGACg                                                                                                                                                                      <  2:419923/251‑1 (MQ=255)                                                                                  cGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGAGGCGCTTGCCGCCCCGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GG‑T‑GGAAAAAATGAGGTTATCCACCGACTTACTCG‑TGTACAAGATACGCGCTCGTTTTAGTCTTGGAGACGGCCAG‑GATTATGAct                                                                                                                                                                      >  2:335744/1‑250 (MQ=255)                                                                                       tGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGGAAATATGGGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGTGGACGGACCG‑GTTTATTACGTaaac                                                                                                                                                                 >  1:307968/1‑249 (MQ=255)                                                                                          ggCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTg                                                                                                                                                              <  1:188865/251‑1 (MQ=255)                                                                                                   cGGCGCGTCAGTCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGc                                                                                                                                                     <  1:185333/251‑1 (MQ=255)                                                                                                         gTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGAtg                                                                                                                                               <  2:109401/251‑1 (MQ=255)                                                                                                              gCACTGTTAAAAAGCGGGCTGGCAGAAACGGCGTGCGGACTAACGATCACTACCGTCTGTGGTTCCGGACTTTCAAGCGTGACGCTTGCAGCCCAGGCCATTCAAGCAGGTCAGGCGAAGAGCATTGTGGCGGCGGG‑CA‑T‑GGACAATATTAGCCTCTCCCCCTACTTATTCGCTGCAAAAA‑CACCCTCTCGTTATCGCCTTGGAAAAGGGCCGCGTTT‑TGACGTCATCCCGCCCGATGGTCTGACGTgcgc                                                                                                                                          >  1:28900/1‑251 (MQ=255)                                                                                                               cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGcc                                                                                                                                         <  1:360189/251‑1 (MQ=255)                                                                                                                    ttAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCCCTGGTTATCGGCTTTGAGACGGACAG‑GGTTATGACGTAATCCTGCGCGATGGCCCGATGTGCGccc‑cccc                                                                                                                                   >  1:348390/1‑250 (MQ=255)                                                                                                                      aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTGCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGAGGGCCTGATGTGCGCCG‑CCCAt                                                                                                                                  <  2:440138/250‑1 (MQ=255)                                                                                                                           gCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTAt                                                                                                                            <  2:78358/251‑1 (MQ=255)                                                                                                                                      gAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGTCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACTGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGAt                                                                                                                 <  1:308318/251‑1 (MQ=255)                                                                                                                                           ggTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GGTTATGACGGAATCCCGCCCGATGGCCTGATGTGTGCCA‑CCCCTGGTTTTAATATGGGGATTTccg                                                                                                            >  1:38295/1‑251 (MQ=255)                                                                                                                                                 cGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑TAGGAAAA‑ATGAGGTTAGCCCCCCACTTAACCGATGCAAAAG‑AAAGCCCTGGTTATCGGCTTGGGGAAGGGCAG‑GGGTATGAAGGAATACTGCGCGATGGCCTGATGTGGGCCC‑CCCATGGTTATCATATGGGGGTTACCGGCGaa                                                                                                       >  2:377761/1‑250 (MQ=255)                                                                                                                                                 cGGATTCACGGTCAATAAAGGATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTGTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCGCTGGTTGTCGTCTTGGAGACGGACAG‑GTTTATGAGGTAAGCCGGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGaaa                                                                                                      <  1:53741/251‑1 (MQ=255)                                                                                                                                                       cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTgg                                                                                               <  1:219303/251‑1 (MQ=255)                                                                                                                                                         cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCt                                                                                             <  1:195823/251‑1 (MQ=255)                                                                                                                                                               aTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTGGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAagag                                                                                       <  2:443236/251‑1 (MQ=255)                                                                                                                                                                 aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGt                                                                                      <  1:272281/250‑1 (MQ=255)                                                                                                                                                                      atggggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTTTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCG‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGa                                                                                 <  2:382220/246‑1 (MQ=255)                                                                                                                                                                        gtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAAt                                                                               <  1:273135/250‑1 (MQ=255)                                                                                                                                                                             tCGGGTCTTCAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GT‑T‑GGAAAATTTTCGGTTAGCCCCCCACCTACTCCATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGGGACGGACCG‑GGTTTTTACGCAATCCTGCGCGGTGGCCTGCTGGGCGCCA‑CCCATGGTTTTCATATTGGGGTTACCCCCGAAAAACG‑GGCTTAAGAGTAAGGAATTAcccc                                                                         >  1:331258/1‑250 (MQ=255)                                                                                                                                                                                ggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAA‑CACGCCCTGGGTATCGTCTTTGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATTGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTg                                                                       >  1:13816/1‑250 (MQ=255)                                                                                                                                                                                      aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑G‑TGAAAAAATTAGGTTAGACCCCCCCTTACTCGATGCAAAAA‑CACGCTCTGGTTTTCGGCTTGGGGACGGGCAG‑GTTTATTACGGAATCCTGCGGGATGGGCCGATGTGCGCCA‑CCCACGGGTTTTATATTGGGGTTAACGCCGAAAA‑CGTGGCCACAGAGGACGGAAATACCCGGGaaaagc                                                                 >  2:115966/1‑247 (MQ=255)                                                                                                                                                                                               gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGGAAATATGAGTTTTGCCCCCCACCTACTCGATGCCAAAG‑CACGCTCTGGTTATCGTCTTTGAGACGGGCAG‑GGTTATGACGTAATCCTGCGCGCTGGCCTGCTGTGCGCCA‑CCCCTGGGTATCATATGGGGCTTACCGCCGCAAA‑CGGGGCTAAAGAGGACCGCATTACCCGTGAAATGCCGGGTGAACt                                                       >  2:96092/1‑251 (MQ=255)                                                                                                                                                                                                        gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTTCGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACa                                              <  1:40774/251‑1 (MQ=255)                                                                                                                                                                                                           cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTc                                           <  1:313135/251‑1 (MQ=255)                                                                                                                                                                                                                 aTTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GGTTATGACGTAATCCCGCGCGATGGCCCGATGTGCGCCA‑CCCATGGTTATCATATGGGGGTTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCg                                     >  1:394756/1‑251 (MQ=255)                                                                                                                                                                                                                    caggcaggTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAAGCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTTGCGCTACATTCACAGCGTa                                   <  2:270805/250‑1 (MQ=255)                                                                                                                                                                                                                    caggcaggTCAGGCGCAGAGCATTGTGGCGGGGGG‑GA‑TTGGAAAA‑ATGGGCTTAGAACCCTACTTTCTCGAGGCAAAAA‑CCCGCCCTGGGTATTGTCTTGGAGAAGTGCAA‑GTTTATTAAGTGATCCCGCGCGATTGGCTCATGTGCGCCAACCCA‑GGTTATCATATGGGGGTTATCGCCGGAAA‑AGTGGGTTGAGAGTAAGGGATTACCCCTGAAATTCAGGATGAACTGACGCCACCTTAACAGCGTa                                   >  2:99203/1‑250 (MQ=255)                                                                                                                                                                                                                       gcaggTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGc                               <  1:140797/251‑1 (MQ=255)                                                                                                                                                                                                                                            gtgGCGGGGGG‑TG‑T‑GGGAAATATGGGTTTTGCCCCCTACTTACTCGATGCCAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGGGCCA‑CCCATGGTTTTCATATGGGGGTTTCCGCCGAAAA‑CGGGGCTAAAGAGTACGGAATTACCCGGGAAATGCAGGAGGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCCCAATTTAGTCCgg          >  2:48807/1‑251 (MQ=255)                                                                                                                                                                                                                                            gtgGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACTTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCg           <  1:349159/250‑1 (MQ=255)                                                                                                                                                                                                                                                    ggg‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTa  <  1:236491/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                              AGTAATTAAAGCCGCCATTGAACGTGCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGGCTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑TA‑T‑GGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG‑CACGCTCTGGTTATCGTCTTGGAGACGGACAG‑GTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCA‑CCCATGGTTATCATATGGGGATTACCGCCGAAAA‑CGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCGCTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTA  >  NC_000913/2326204‑2326697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑