Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,559	0	G	T	33.3%	56.4 / 14.1	39	H16Q (CAC→CAA)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base G (6/20);  new base T (13/0);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 3.34e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAATCTCTTTTTCGATCCACGAGACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAA  >  NC_000913/862309‑862799                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       caATCTCTTTTTCGATCCACGAGACAGTATATTCCGGGAAGATCGGCGCGTCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGGTTCACAATAATCACCCGCGCAGTTTtg                                                                                                                                                                                                                                                        >  2:36061/1‑251 (MQ=255)         ttttCGATCCACGAGACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCtt                                                                                                                                                                                                                                                 <  1:206526/250‑1 (MQ=255)             cGATCCACGAGACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATg                                                                                                                                                                                                                                           <  2:462861/251‑1 (MQ=255)              gATCCACGAGACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGc                                                                                                                                                                                                                                          <  1:29249/251‑1 (MQ=255)                 ccACGAGACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGt                                                                                                                                                                                                                                       <  2:10992/251‑1 (MQ=255)                               atTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCCCCCCCCCCTTTTTTTTCGCTTT‑TATTCGGTCGCTGTGCgttttc                                                                                                                                                                                                                         >  1:311862/1‑247 (MQ=255)                               atTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTc                                                                                                                                                                                                                          <  2:31123/250‑1 (MQ=255)                               atTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTc                                                                                                                                                                                                                          <  2:452893/250‑1 (MQ=255)                                                      cgAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGgttgt                                                                                                                                                                                               <  1:387185/251‑1 (MQ=255)                                                             cGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCCTTTTTTTGCGCTTT‑TATGCGGGCGCTGAGCGTGTCCA‑G‑TTTTCGTGTGG‑TCATGGTTGTTATCCTc                                                                                                                                                                                        >  1:130790/1‑251 (MQ=255)                                                                  gCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTTCCATATGCACCAGCGCCTTTTTTTTGGCTTT‑TAGTCGGTCGCTCGGCGTGTCCA‑G‑TTTCTGTGTGG‑TGCTGgttgtttttctcttt                                                                                                                                                                                     >  2:455943/1‑240 (MQ=255)                                                                   ccTGGTTGCCAATGATCAACTCGTCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGACCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAg                                                                                                                                                                                   <  2:136157/250‑1 (MQ=255)                                                                           ccAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTACAATATACACCACCGCCTTTTTTTGCGGTTT‑TATGCGGGCGCTGCGCGTGTTCC‑G‑TTTTAATGTGG‑TGTTGGTTGTTTTTCTCGTTAGGTTGCgggt                                                                                                                                                                          >  2:405732/1‑249 (MQ=255)                                                                            cAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTa                                                                                                                                                                        <  2:420548/251‑1 (MQ=255)                                                                               tGATCAACTCATAGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGGTTCTCTACATGCCCCAACGCCTTTTTTTGGGCTTTTAATG‑GGTCGCTGAGCGTGTGCA‑GGTTTC‑GGGTGG‑GCATGGTTGTTTTCCTTGTAAAGTCGCGG‑GTAAcc                                                                                                                                                                     >  2:448074/1‑251 (MQ=255)                                                                                      cTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTTCCATATGCCCCCGCGCCTTTTTTTGGGCTTT‑TATGCGGGTGCCGAGCGGGTCCA‑G‑TTTTTGTGTGG‑TCATGGTTTTTTTCCTCGTTAGGGCGCGGGTTAACCCttttt                                                                                                                                                               >  2:43670/1‑249 (MQ=255)                                                                                        cATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCTCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGTGG‑TTAACCCTTTCTggc                                                                                                                                                            <  2:83186/251‑1 (MQ=255)                                                                                                     ccAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑GCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATActgct                                                                                                                                                <  2:420694/250‑1 (MQ=255)                                                                                                           ggTGCGATTCGCCAGGTGATGCGCCATTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCCCAATATTCACACGCGCATTTTTGTGGGCGTT‑TATGAGGGCGCTGAGCGTGTCCC‑G‑CTTCTGTGTGG‑TCATGGTTGTTTTACTCCTAAGGGCGCGG‑TTAACCCttttttgtgggcatacccctggggga                                                                                                                                          >  2:139324/1‑227 (MQ=255)                                                                                                           ggTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCaaa                                                                                                                                         <  1:6965/251‑1 (MQ=255)                                                                                                                  ttCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATAAGCCCCAGCCCCTTTTTTTTCGCTTT‑AATTCGGGCGCTTAGCGGGTCCA‑G‑TTTCAGTGTGGGTC‑TGGTTTTTTTTCTCCTAAGGTCGCGG‑TTTACCCTTTTTTGCCGGCATTCTTCTGGGCGAAGGCGAGc                                                                                                                                  >  2:298105/1‑251 (MQ=255)                                                                                                                    cGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATTCACCAGCGCATTTTTTTGCGCTTT‑TATGCGGTCGCTGAGCGGGTCCA‑G‑TTTCAGTGTGG‑TCATGGGTTTTTTCCTCGTAAGGGCGCGG‑GTAACCCTTTCTGGCAGGCATACTGCTGGGCCAAGTCGAGc                                                                                                                                  >  1:434626/1‑249 (MQ=255)                                                                                                                                         cAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGttt                                                                                                          <  2:434626/251‑1 (MQ=255)                                                                                                                                                    gTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTTCCCATATGTCCCAACGCATTTTTTTGCGCTTT‑TATGCGGGCGCTGAGCGTGTCCA‑G‑TTTTAGTGTGG‑GGTTGGTTGTTTTCCTCGTAAGGTCGCGG‑TTAACCCCTTCTTGCAGGCCTAATGGTGGGCAAAGGCCAGCAGTTTCGGCGGATCAAGAGGGTTTTTCCgggcgg                                                                                                >  2:227561/1‑248 (MQ=255)                                                                                                                                                                   tATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGAtt                                                                                  <  2:293266/249‑1 (MQ=255)                                                                                                                                                                   tATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGAtt                                                                                  <  2:244127/249‑1 (MQ=255)                                                                                                                                                                      cGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATtaagta                                                                             <  1:448074/251‑1 (MQ=255)                                                                                                                                                                          aTGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTTCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTg                                                                         <  1:269956/251‑1 (MQ=255)                                                                                                                                                                                ttgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACCATATTCACCCGCCCCTTTTTTTGCGGTTT‑TATGCGGGCGCTGAGCGTGTCCCCG‑TTT‑AGTGTGG‑GCCTGGTTGTTATCCTCGTAAGGGCGCGG‑GTTACCCCTTCTTGCAAGCATAATTCTGGGGAAAGTCGAGCAGTTCTTGCGCACCCAG‑CGGGTTTTCCGGGGGGTCCTAGGGGAGATtatgggagagatattt                                                                   >  2:319227/1‑237 (MQ=255)                                                                                                                                                                                 tgATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATAttt                                                                   <  2:130790/250‑1 (MQ=255)                                                                                                                                                                                         ctcGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAAAAAGCACCCGCGCATTTTTTTGCGCTTT‑TATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCCGGGTGG‑TCATGGGTGTTATTCTCGTAAGGTCGCGG‑TTAAACCTTTTTGTCCGGCATACTGCTGGGCAAAATCGCGCAGGTCTTGTGCCTCAAA‑CGGTTTTTCCTGGGCGTCATCGGGCCGATTAAGGAAGTGATATTTGTTGATGcc                                                          >  1:32324/1‑251 (MQ=255)                                                                                                                                                                                                 aGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCCGCGCATTTTTTTGCGCTTT‑AATGCGGTTGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑GTAACCCTTTCTTGCCGGCATACTTCTGGGCAAAGTCGAGCAGTTCTGGCGCCTCAAG‑CGGTTTTTCCCGGGCGTCATAGGGGCGATTAAGTAAGGGTTATTTTTTTATGCCCAGCgtgt                                                  >  2:338577/1‑251 (MQ=255)                                                                                                                                                                                                            cgcTCGGTACAGACTGGCGGTTTTACAAAATGCACCAACGCATTTTTTTGCGCTTT‑AAAGCGGTCGCTGAGCGTGGCCA‑G‑TTTCAGTGTGG‑TCATTGTTGTTATTCTCGTAAGGGCGCGG‑GTTACCCCTTCTGGCAGGCATACTGCTGGGGAAAGTCGAGCAGGTCTGGGGCATCAAG‑CGGGTTTTCCGGGGGGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGTCCAGCGTGTgggggggcaa                                        >  2:354833/1‑242 (MQ=255)                                                                                                                                                                                                                            gCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCGTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCaa                       <  2:155622/251‑1 (MQ=255)                                                                                                                                                                                                                                   cacaATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGc                  >  1:316698/1‑249 (MQ=255)                                                                                                                                                                                                                                    acaATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGc               <  1:36587/251‑1 (MQ=255)                                                                                                                                                                                                                                     caATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCt              >  2:254376/1‑251 (MQ=255)                                                                                                                                                                                                                                     caATATGCACCAGCGCATTATTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGACTTTCGCCAACGTGCAGCt              >  2:464660/1‑251 (MQ=255)                                                                                                                                                                                                                                                aGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCggcggc    >  2:198160/1‑250 (MQ=255)                                                                                                                                                                                                                                                  cgcATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCaa  >  2:339726/1‑250 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       CAATCTCTTTTTCGATCCACGAGACAGTATATTCCGGGAAGATCGGCGCGGCGCGAACTTCGCTTGCCTGGTTGCCAATGATCAACTCATCGTGTTTGATCCAGATGGTGCGATTCGCCAGGTGATGCGCCAGTGCCAGCGCGCGACGTACCGGGATCGGCTTATCGAGATGTTGTTGATACATCTCGGTATAGTGCTGCGCGCGCTCGGTACAGACTGGCGGTTTCACAATATGCACCAGCGCATTTTTGTGCGCTTT‑AATGCGGTCGCTGAGCGTGTCCA‑G‑TTTCAGTGTGG‑TCATGGTTGTTATCCTCGTAAGGTCGCGG‑TTAACCCTTTCTGGCAGGCATACTGCTGGGCAAAGTCGAGCAGTTCTGGCGCATCAAG‑CGGTTTTTCCGGGGCGTCATAGGGCAGATTAAGTAAGTGATATTTGTTGATGCCCAGCGTGTGGTAGGGCAGAAAATGAATTTCGCCAACGTGCAGCTCGTCGGCGGCAA  >  NC_000913/862309‑862799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑