| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 657,676 | 0 | A | C | 36.6% | 60.9 / 13.5 | 42 | V88G (GTG→GGG) | crcB | F(‑) channel protein |
| Reads supporting (aligned to +/- strand): ref base A (21/5); new base C (2/13); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.30e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCT > NC_000913/657433‑657921 | cAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTc > 1:146121/1‑251 (MQ=255) gcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGcc < 2:6382/251‑1 (MQ=255) gcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGcc < 1:250943/251‑1 (MQ=255) cgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCg > 2:271927/1‑251 (MQ=255) aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGcc > 2:370558/1‑251 (MQ=255) gCCCTTCTGCTGCAGACGTCATCGCACTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTCATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGATGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAACATGTTGAGAAGGTTGTTAGACCGCcaca > 2:194267/1‑250 (MQ=255) ccaaccGTAATCGCTCTGTAAGATACGTCAGAAAGAATTCAAACCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAACCCCC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCggtggt < 1:180206/246‑1 (MQ=255) aagttAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTAAATCAGGGGGTTTTTTTTGGTCTTTGG‑TGTGCGGTGGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAAAAAAAACCCC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTAAGACCGCCACAAAATCCGGTGGTGAt < 1:313355/247‑1 (MQ=255) tAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGAGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt > 1:291665/1‑251 (MQ=255) ctttAAGATACATCAGCAAAAATTCAAACCCCGCTTAAGCAGGGGGTTTTTTTGGTTCTTTGG‑TGGGCGGTTGAGGCCAAAACCACCCAGAATCCCAGTGCTGTCAGGGCAAAAGCCCCCAGAAGTTTGACTAAACCGTTCAGGATTGCGCGGCCAAAGCGGCGCGTTTT‑TAACAAAAACCCC‑ACTTCTCCGGAAAATTGTGAGAAGGTTGTTATACCGCCACAAAATCCGGTGGTGATTAATACTTTc < 1:422743/246‑1 (MQ=255) gaaagttACGTCAGCAAGATTTAAAACCCCGCTTAAGCAGGGTGTTTTTTTTGGTCTTTAG‑TGTGCGGTGGAGGCCAAAAACAGCCAGAATGCCGGGGGGGTCATGGCAAAAGCCCCAAGAAGGTTGACGAAAACGTCCAGTAATCCCAAGCCAAAGCGGCCCT‑TTT‑TAAAAAAAACCCCCACTTCTGCGAAAAATGTTGAGAGGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCcaca < 2:348233/245‑1 (MQ=255) gaaaGATACGTCAGCAAAAATTCAAAACCCCTTTAACCAGCGGGTTTTTTTTGGT‑TTTAGGTGGGCGGTTGAGGCCGAAACCAGCATGAATGCCATTGCGGTAATGGCAAAAGACCCCAGAGGGTTGACAAAACCGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCAAAATTGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTcc < 1:341184/246‑1 (MQ=255) tcaagaaaaaataaaaaccccGCTTAACCGGCGGGTTTTTTTTGGTCTTTAG‑GGGGCGGTGGAGGCCGAAAACAGCCAGAAGGCCAGTGCGGTAAGGGCAAAAGCCCCCAGAAGGTAGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCTTCTTG‑AAACAAAACCCCC‑ACTTCTGCCGAAAATGTTGAGAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCa < 2:59829/232‑1 (MQ=255) gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCa > 2:124072/1‑250 (MQ=255) aGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCa > 1:59829/1‑247 (MQ=255) aaaGAATTCAAAACCCGCTGAATCGGCGGTTTTTTTTTGGTCTTTGG‑TGTGCGGTTGAGGCAGAAAACAGCCAGAATGCCAGTGCGGTAATGGAAAAAGACCCAAGAGGGTTGACAAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTCTTG‑TAACAAAAACCCC‑ACTTCTGCCAAAAATGTGGAAAAGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTc < 2:336302/250‑1 (MQ=255) aTTCAAAACCCGTTTAAGCGGGGGGTTTTTGTGGGTCTTTGG‑TGTGCGGTTGGGGCCAAAAACAGCAAGAATGCCAGGGCGGACAGGGCAAAAGACCACAGAAGGTTGAAGAAAACGTTCAGTAATGCCCAGCAAAAGCGGCCCTTTTG‑AAACAAAAACACC‑CCTTCTGCCGAAAATGTTGAAAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCAt < 2:58854/251‑1 (MQ=255) aaaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTGGAGGCCGAAAACAGCCAGAAGGCCAGTGCGGTCATGGAAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACCCC‑ACTTCGGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCct < 1:136152/250‑1 (MQ=255) aaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCctgc > 1:249649/1‑251 (MQ=255) tAATCAGCGGGTTTTTTTTGGTCTTTAG‑GGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGCCCCCAGAAGTTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGCCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCAt < 1:271927/250‑1 (MQ=255) atccAGCGGGTTTTTTTTGTTCTTTAG‑TGTGCGGTTGAGCCCAAAAACAGCCAGAAGGCCAGTGCGGTCAGGGAAAAAGACCCGAGAAGGTTGACGAAAACGTTCAGTAATGCCCTGCCAAAGCGGCCCTCTTG‑TAACAAAAGCTCC‑CCTTCTGCCGAAAATGGTGAGATGGTTGTGAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGc < 1:22223/248‑1 (MQ=255) accgttttttttttGGTCTTTTG‑TTTGCGGTTAAGGCCAAAAAAACCCAGAATGCCAGTGGGGTCAGTGCAAAAGCCCCCAGAAGGTTGACAAAAACGTTCAGTAATGCCCAGCAAACGCGCCCCT‑TTGATAACAAAAACCCC‑ATTCCCGCGAAAAATTTTGAGAAGGTTTTAAGTCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAt < 1:430017/245‑1 (MQ=255) aGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAt > 1:364779/1‑251 (MQ=255) ggtttttAG‑TGGGCGGTTGAGGCCGAAAACAGCCAAAAGGCCAGTGCGTTCATGGCAAAAGCCCCCAGAAGTTTGACAAAAACGTTCAGTAATGCCAAGCCAAAGCGGCCCTCTTG‑TAACAAAACCCCC‑ACTTCTGCCGAAAATGTTGAGAAGGTTTTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTatgat < 1:124615/247‑1 (MQ=255) tgtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCGGCTGAACCATGCGAATCCTATTCCTTTGATGAATGcccc > 1:20673/1‑251 (MQ=255) gtgCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAACATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTCTTCCTATGATGAATGcccc > 1:295474/1‑250 (MQ=255) cGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCctgc > 1:329688/1‑210 (MQ=255) aCAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGAtt > 1:35248/1‑158 (MQ=255) gCGGTCATGGCAAAAGACCCCAGAAGGTTGACGCAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACACAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGAATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGACTGCCCCAATCAGGTTTGCTGTCATCGTCCCCAACGGAATCGc > 2:238505/1‑251 (MQ=255) cGGTCATGGCAAAAAACCCCAGAAGGTTGACAAACCCGCTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑CAACAAAAACCCC‑CCTTCTGCCGACAATGTTGCGAAGGTTGTTAGACCGCCACAAAATCCGGTGGCGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCGCAACGGAATCGcc > 2:51153/1‑251 (MQ=255) ggTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACGAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCt > 2:362131/1‑251 (MQ=255) gtaaggacAAAAGCCCCCAAAAGGGTGAAAAAAACGTTCAGAATTCCCCATACAAACCGTCCCTCTTG‑AAAAAAAAACCCC‑ACTCCTGCCAAAATTTTTGAAAAGGTGGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTGCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTAGTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTg < 2:295474/244‑1 (MQ=255) gTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTg > 2:340531/1‑251 (MQ=255) agggCAAAAGCCCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑AAACAAAAACCCC‑ACTGCTGCCAAAAATGTTGAGAAGGTTGTAAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATg < 1:299482/249‑1 (MQ=255) aaaCGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGc > 1:103767/1‑251 (MQ=255) gCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGAGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCt > 1:379980/1‑251 (MQ=255) ccaaaaGCGGCCCTTTTG‑AAACAAAAACCCC‑ACTTCTGCCGAAAATGTTGAGAAGGTGTTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCg < 1:370558/248‑1 (MQ=255) cAAAGCGGCCCTCTTG‑TCACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATAATTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCACGt > 2:74283/1‑250 (MQ=255) aGCGGCCCTCTTG‑TAACAAAAACCCC‑CCTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc < 1:86811/142‑1 (MQ=255) aGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcc > 2:86811/1‑142 (MQ=255) gCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGc > 2:384281/1‑251 (MQ=255) aaaCCCC‑ACTTCTGCCGAAAATGTTGAGCAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTACTACTTTCCACACTGGAACAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTACGCGTCACCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCt > 2:385741/1‑250 (MQ=255) | CAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAG‑TGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTG‑TAACAAAAACACC‑ACTTCTGCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCT > NC_000913/657433‑657921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |