Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,375,823	IS186 (+) +9 bp :: Δ5 bp	coding (132‑140/963 nt)	menC ←	o‑succinylbenzoate synthase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2375823 =	0 (0.000)	6 (0.130)	6/492	3.1	100%	coding (140/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	= 2515612	NA (NA)					noncoding (1340/1345 nt)	IS186	repeat region
Rejected: Coverage evenness skew score above cutoff.
*	?	NC_000913	= 2375831	0 (0.000)	43 (0.930)	40/484	0.3	100%	coding (132/963 nt)	menC	o‑succinylbenzoate synthase
	?	NC_000913	2514273 =	NA (NA)					noncoding (1/1345 nt)	IS186	repeat region

CACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2376061‑2375823 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTT  <  NC_000913/2515612‑2515464                                                                                                                                                                                                                                                                                                                                                                                                      CACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGGGAAGGCGAGCGCGGAGGGGGGGGGGGGATCTCCCCTAAGCGCTAACT                                                                                                                                           >  1:372226/1‑251    CATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGGGAAGGCGAGCGCGAAGGGTGGGGGGGGATCTCCCCTAAGCGCTAACTTA                                                                                                                                         >  2:115850/1‑250                CATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGGTTGCCTGCGTGAAGGCGAGCGCGGAGGGGGGGGGGAGATCTCCCCTAAGAGCTAACTTACGGGTTGAACCA                                                                                                                             >  2:95104/1‑250                                    AAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATAGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCCCGACGGGGTGTGTGTTTGCCTGCGTGAAGGGGAGCGCGGAGGGGGGGGGGGGAGCTCCCCTAAGCGCTCAATTAAGGGGTGAAACATCcggagaaatcggcgccccg                                                                                                        >  2:169649/1‑232                                                                                                                           CTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGGGATCTCCCCTAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTTTAGGCTGTCAGGGATACTGTCCCACGAATGGCCCCCTGTAAGC                 >  1:110855/1‑251                                                                                                                                          GGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGGGGAGGCGAGCGCGAAGGGGGGGGGGGGATCTCCCCTAAGCGCTAACTTAAGGGGTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTTAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCCGGGATACTGTCCCCCGCATGGCCCCCTGTAAGCTCCAGATGACCATTT  >  2:87387/1‑251                                                                                                                                                                                                                                                                                                                                                                                                      CACCATGCTGTTCTACATGACGGAAGAAGGTCAGGAAGGTCGCAACGCCTTCAACCAGAAACGTCAGCCTGACTTCAGCAAATTCAAACGGAATCCGTAATGCGTAGCGCGCAGGTATACCGCTGGCAGATCCCCATGGACGCGGGGGTGGTTCTGCGCGACAGGCGGTTAAAAACCCGCGACGGGCTGTATGTTTGCCTGCGTGAAGGCGAGCGCGAAGGGTGGGGGGAGATCTCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2376061‑2375823 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAAGCGCTAACTTAAGGGTTGAACCATCTGAAGAATGCGACGCCTCGGTGCCTCGTTAAGACGATGCCTCGCGTTCTTCAATTGCGTTTTGTAGGCTGTCAGGGATACTGTCCCACGAATGGCCACCTGTAAGCTCCAGATGACCATTT  <  NC_000913/2515612‑2515464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.