Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,813 Δ2 bp coding (142‑143/1161 nt) metB → O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,8110C.96.7% 266.3 / ‑0.6 60coding (140/1161 nt)metBO‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (27/31);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
*NC_0009134,128,8120G.96.7% 264.9 / ‑2.3 60coding (141/1161 nt)metBO‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base . (27/31);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAGGCGATGA  >  NC_000913/4128564‑4129051
                                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                               
aaTCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACAACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtc                                                                                                                                                                                                                                               <  2:360232/251‑3 (MQ=255)
  tCTATACGCAAAGAAGTTTAGATGTCCCGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcgc                                                                                                                                                                                                                                             >  1:359194/1‑247 (MQ=255)
  tCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcgc                                                                                                                                                                                                                                             >  1:45454/1‑247 (MQ=255)
     ataCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcg                                                                                                                                                                                                                                              <  2:134413/247‑4 (MQ=255)
     ataCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTACATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACtcgtcgcggc                                                                                                                                                                                                                                          >  1:433125/1‑244 (MQ=255)
               aagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAAcgc                                                                                                                                                                                                                              >  1:338374/1‑251 (MQ=255)
                   ttAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAAcgcgcg                                                                                                                                                                                                                           <  1:188164/250‑1 (MQ=255)
                   ttAGATATCCAGATTTATTGACGTCCATTAACACACTGTTTACTCTGGTGCCTGACATTTCACCGCCAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTCGCGGGTTAACTGACGACGAACAGTCTGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTCCCGGATTTACTGAACCGCGCTCGCATGATTACT‑‑CGACTCGGCCACCCAAcgcgcgc                                                                                                                                                                                                                          >  2:200852/1‑250 (MQ=255)
                     aGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGAtg                                                                                                                                                                                                                        >  2:192204/1‑251 (MQ=255)
                        tGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTgg                                                                                                                                                                                                                     >  1:301827/1‑251 (MQ=255)
                           ccAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTc                                                                                                                                                                                                                  >  1:182480/1‑251 (MQ=255)
                            cAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCa                                                                                                                                                                                                                 <  2:15588/251‑1 (MQ=255)
                               aTGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCg                                                                                                                                                                                                              >  1:100474/1‑251 (MQ=255)
                               aTGTATTGACGTCAATTAACACAATGTTTCCTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCg                                                                                                                                                                                                              >  2:379490/1‑251 (MQ=255)
                                   aTTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGAGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTgcg                                                                                                                                                                                                          >  1:96098/1‑251 (MQ=255)
                                          tCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAACCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGCCGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTACTGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGc                                                                                                                                                                                                     >  2:464953/1‑249 (MQ=255)
                                                aaCACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTg                                                                                                                                                                                              <  1:87479/250‑1 (MQ=255)
                                                               ctGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGAGGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctgg                                                                                                                                                                                <  1:21679/249‑1 (MQ=255)
                                                                 ggTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgctggt                                                                                                                                                                               <  1:378512/248‑1 (MQ=255)
                                                                        gACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGtact                                                                                                                                                                       <  1:345467/249‑1 (MQ=255)
                                                                           aTTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTa                                                                                                                                                                  <  1:193099/251‑1 (MQ=255)
                                                                                aCCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCTCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATAcc                                                                                                                                                             <  1:210604/251‑1 (MQ=255)
                                                                                      aaaGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGAGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGACATGGAAGGAGGTGCTGGTGCAGTACTTACTAATACCGGcctg                                                                                                                                                       >  2:239733/1‑248 (MQ=255)
                                                                                           ccAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCcgc                                                                                                                                                  >  1:6967/1‑251 (MQ=255)
                                                                                           ccAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCcgc                                                                                                                                                  <  2:330368/251‑1 (MQ=255)
                                                                                               ggAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGAtt                                                                                                                                              <  1:4815/251‑1 (MQ=255)
                                                                                                gAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTc                                                                                                                                             >  1:253763/1‑251 (MQ=255)
                                                                                                     tcatcaCATGACGCGTAAACAGGCCACCAGCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCAcc                                                                                                                                          <  2:373232/249‑1 (MQ=255)
                                                                                                       atcaCATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGt                                                                                                                                      <  1:236116/251‑1 (MQ=255)
                                                                                                         cacaTGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTa                                                                                                                                     >  1:35724/1‑250 (MQ=255)
                                                                                                          acaTGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAAc                                                                                                                                   >  2:193143/1‑251 (MQ=255)
                                                                                                          acaTGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAAc                                                                                                                                   <  2:301827/251‑1 (MQ=255)
                                                                                                                    tAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCttt                                                                                                                         >  1:120955/1‑251 (MQ=255)
                                                                                                                        cAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGATGCGTTGTCCCACCGATCCATCGTTCCAGCACCTATAACTTTGCCGGATTTAATGAACCGCGCGCGCATGATTA‑‑CGCGGCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCGGGTTTAGCACGTGTTAATACCGGGATGTCCGGTATTCCGCTGTTAAGAACCGTCTTTTTGa                                                                                                                     <  2:211475/251‑1 (MQ=255)
                                                                                                                         aGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGaa                                                                                                                    <  1:231254/251‑1 (MQ=255)
                                                                                                                          ggCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGaaa                                                                                                                   <  1:296381/251‑1 (MQ=255)
                                                                                                                            ccaccaTCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAAcc                                                                                                                 <  1:101197/251‑1 (MQ=255)
                                                                                                                             caccaTCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCt                                                                                                                >  1:166399/1‑251 (MQ=255)
                                                                                                                                 aTCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCg                                                                                                            >  2:213768/1‑251 (MQ=255)
                                                                                                                                           gTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGt                                                                                                  <  1:464953/251‑1 (MQ=255)
                                                                                                                                            tAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTACTGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTACTACCGGCCTGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGtt                                                                                                 >  1:287408/1‑251 (MQ=255)
                                                                                                                                                         gacgacGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGAc                                                                                     <  2:338374/250‑1 (MQ=255)
                                                                                                                                                                 aCAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggc                                                                            >  1:346953/1‑251 (MQ=255)
                                                                                                                                                                  cAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggcg                                                                           <  1:340262/251‑1 (MQ=255)
                                                                                                                                                                   aGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggcgg                                                                          >  2:117176/1‑251 (MQ=255)
                                                                                                                                                                    gTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTAcggcggg                                                                         >  2:121548/1‑250 (MQ=255)
                                                                                                                                                                        ggTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGc                                                                      <  2:182480/250‑1 (MQ=255)
                                                                                                                                                                           ggCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATc                                                                  <  1:417731/250‑1 (MQ=255)
                                                                                                                                                                                    ccACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTcc                                                         >  1:132116/1‑250 (MQ=255)
                                                                                                                                                                                          aTCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAggc                                                   >  1:99205/1‑249 (MQ=255)
                                                                                                                                                                                                  ttCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGaa                                            >  1:314144/1‑250 (MQ=255)
                                                                                                                                                                                                    ccAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAAcg                                         <  2:6967/251‑1 (MQ=255)
                                                                                                                                                                                                    ccAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAAc                                          <  2:92475/250‑1 (MQ=255)
                                                                                                                                                                                                    ccAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAAc                                          <  1:65615/250‑1 (MQ=255)
                                                                                                                                                                                                               aaCTTTACCGGATTTAATGAACCGCGCGCGCATGATTA‑‑CGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATc                              <  2:244495/251‑1 (MQ=255)
                                                                                                                                                                                                                       cGGATTTAAGGAACCGCGCGCGAATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGtgttg                      <  2:461404/251‑1 (MQ=255)
                                                                                                                                                                                                                        ggATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgct                                                                                                                                                                                  <  2:414593/94‑1 (MQ=255)
                                                                                                                                                                                                                        ggATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGtggtgct                                                                                                                                                                                  >  1:414593/1‑94 (MQ=255)
                                                                                                                                                                                                                         gATTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTgttgtt                    <  2:100474/251‑1 (MQ=255)
                                                                                                                                                                                                                          aTTTAATGAACCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGttgttt                   <  1:117176/251‑1 (MQ=255)
                                                                                                                                                                                                                                  aaCCGCGCGCGCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCa           <  2:279640/251‑1 (MQ=255)
                                                                                                                                                                                                                                    ccGCGCGCTCATGATTACT‑‑CGTCGCGGCAACCCAACGCGCTATGTGGTTCAGCGTCCTCTGGCATAACTGGAAGGTGGTGCTGGTGCACTACTTACTAATACCGTCATGTCCTCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAg         <  2:45454/251‑1 (MQ=255)
                                                                                                                                                                                                                                            gcATGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAACTATCCCCTGTTCGACATGCTGGCGAAAACCGGTTGCCATCGCCGGTTGTTTTTTGTTCAAGGCGATGa  >  2:394462/1‑250 (MQ=255)
                                                                                                                                                                                                                                               tGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGt                                                                         <  2:460615/176‑1 (MQ=255)
                                                                                                                                                                                                                                               tGATTACT‑‑CGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGt                                                                         >  1:460615/1‑176 (MQ=255)
                                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                               
AATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCATCTTTCCAGCACCTATAACTTTACCGGATTTAATGAACCGCGCGCGCATGATTACTCGCGTCGCGGCAACCCAACGCGCGATGTGGTTCAGCGTGCGCTGGCAGAACTGGAAGGTGGTGCTGGTGCAGTACTTACTAATACCGGCATGTCCGCGATTCACCTGGTAACGACCGTCTTTTTGAAACCTGGCGATCTGCTGGTTGCGCCGCACGACTGCTACGGCGGTAGCTATCGCCTGTTCGACAGTCTGGCGAAACGCGGTTGCTATCGCGTGTTGTTTGTTGATCAAGGCGATGA  >  NC_000913/4128564‑4129051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑