Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.92.5% 164.0 / 4.8 40intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base . (24/13);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 5.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,173,3640C.94.9% 163.4 / 4.2 40intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base . (24/13);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

ACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCG  >  NC_000913/2173149‑2173613
                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                                         
acaaaTAGGTTGCGTCGCGATCAACGGGGTGATGCTAATAATGATGCCACCCGAGATTAAGGTGCGGAACAGATTCCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                                                                                                                                                      <  2:466593/248‑1 (MQ=255)
 ccAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                                                                                                                                     >  2:458706/1‑251 (MQ=255)
         ttGCGTCGCGATCCACAGGGTGATGCTCATAATGATGCCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACt                                                                                                                                                                                                             >  2:226094/1‑251 (MQ=255)
                gcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc                                                                                                                                                                                                      >  1:89811/1‑251 (MQ=255)
                    tCCACGGGGTGATGCTCAAAATGATGACCCCCGAGATTAAGGTGCGAAACAGATTCCCACGAGGCCCGGCGCCCGCCACCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                                   <  2:389170/250‑1 (MQ=255)
                                  cTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                                     >  2:364840/1‑250 (MQ=255)
                                       aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGACACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGCATCAAGGCCAATCAGCAACTCCTGCCCGCTGACCTTCTCCTGCAACCGAc                                                                                                                                                                               >  2:184747/1‑251 (MQ=255)
                                                          aaGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGAt                                                                                                                                                            >  2:215834/1‑251 (MQ=255)
                                                           aGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATg                                                                                                                                                           <  1:30904/251‑1 (MQ=255)
                                                              ggCGGAACAGATTTCCCCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGc                                                                                                                                                        <  1:400794/250‑1 (MQ=255)
                                                                    aCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTaaa                                                                                                                                                  <  1:215834/251‑1 (MQ=255)
                                                                           tCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAccatcc                                                                                                                                            <  1:327878/250‑1 (MQ=255)
                                                                            ccACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCACACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGATTGCTTAGCGATGAGCGTTAAAccatccat                                                                                                                                          >  2:252758/1‑251 (MQ=255)
                                                                               cGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatgat                                                                                                                                       >  1:266318/1‑251 (MQ=255)
                                                                                                 aTCGCAACGAAGACGCCGATGACGGCACGGTAGCCAAACGGCAGCACCTGACTAACCGGCACACACACAGCAATTCAAATGC‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACACCGGATAAAGGACAATCAGGAACTTCTGCCAGCCGCACTTCGCCTGTAACAGACTACGAGCTTTCTTAGCGCGGGTCGTTAAACCATTCATGATGGGTTTAATCACCCTTgg                                                                                                                     >  2:426292/1‑251 (MQ=255)
                                                                                                     ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                 >  1:373966/1‑251 (MQ=255)
                                                                                                          aagaagCCGAGGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAAAAGAAATTAAAAGGG‑TGAGTGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagcag                                                                                                            <  2:263951/251‑1 (MQ=255)
                                                                                                            gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAgcagca                                                                                                          <  1:351805/251‑1 (MQ=255)
                                                                                                                  gagggtgcaaaGATCGCCAAACGGCAGCACCGGATTACCCGGCACCCAAACAGCAATTAAAAGGG‑TGATTGGGATAAAACTCGGTCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCTAATCAGGAACTCCTGCCCGGCGAACGTCTCCTGTAAACGTCTAGGAGTTTGCTTAGCTATGTGCTTTAAGCGTTCCTGGATGGGTTTAATCCCCCGTGGCATTAGCATCATCACTg                                                                                                    <  1:39930/242‑1 (MQ=255)
                                                                                                                        ggaaaGATCCCAAAAGGGCAGCACCAGATTACCCGGCACACAAACAG‑AAATAAAATGGGTGAGTGGGAAAAAAACCAGGCTGGCCGAT‑CC‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGccgccg                                                                                              <  2:184061/248‑1 (MQ=255)
                                                                                                                          aaaGATCGCCAACCGGCAGCCCCTGATTCCCCGGCACAAAAACAGAAATTAAAATGG‑TGAGTGGGAAAAAAATCAGGCTGCCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtt                                                                                            <  1:355877/250‑1 (MQ=255)
                                                                                                                                           agcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAGCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCa                                                                            >  2:441778/1‑250 (MQ=255)
                                                                                                                                            gcaCCTGATTCCCCGCCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAAt                                                                          <  1:236795/251‑1 (MQ=255)
                                                                                                                                            gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAAt                                                                          >  1:325364/1‑251 (MQ=255)
                                                                                                                                                 tGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGCGTGGGCTAACAATCAGGCTTGCCGATACAACC‑‑GCCGTATGTCCCAACAACAACGCCGGATCAAGACCAAACCGGAACTCCTGACCGCCGAACTTCGACTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATACATGATGGGTTTAATCACCCGTGGCATTAGCAGCAACACTGCCGCCGTTTTTACCGCCAGCTGCAATACAcc                                                                     >  1:440733/1‑251 (MQ=255)
                                                                                                                                                          gggacccgaatcgCAATCAAAATGG‑CGAGCGGGATAACAATCAGGCTTGCCAATACCACC‑‑GCCGTATGTCCCAGCCGCAACGCCGGATCCAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTAACCGACTAAGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGCTTAATCACCCCTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACACTTGACATc                                                            >  2:67827/13‑251 (MQ=255)
                                                                                                                                                           gCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCg                                                           >  1:115684/1‑251 (MQ=255)
                                                                                                                                                                          ttAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATg                                            >  2:140338/1‑251 (MQ=255)
                                                                                                                                                                            aaaaatgggtgttggaaaaaaaaTCAGGCTTGCC‑ACACC‑CCCTGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTAGCCGCCGAACTTCGCCTGTAAACGACTCCTAGCTTGCTTAGCGATGGGCTTTAAACCTTCCATGATGGGTTTAATCACCCGTGGCATTTGCAGCATCACTGCCGCCGTTTTTACCGCCATCTGCTATACACCTTTTATATCGTAACCCGCGAGGATg                                            <  2:226752/232‑1 (MQ=255)
                                                                                                                                                                            aaaaagggtgggggggaaaaaaacgaGGCTTGCGGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGAGGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATg                                            <  2:120285/225‑1 (MQ=255)
                                                                                                                                                                             aaaTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGCTCCCAAC‑‑GCCGACTGTCACAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATAGTAACCCGCGAGGCTGCCg                                         >  1:421167/1‑251 (MQ=255)
                                                                                                                                                                             aaaTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                         >  1:82858/1‑251 (MQ=255)
                                                                                                                                                                              aaTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                         >  1:235071/1‑250 (MQ=255)
                                                                                                                                                                               aTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                       >  1:2727/1‑251 (MQ=255)
                                                                                                                                                                               aTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                       >  1:255997/1‑251 (MQ=255)
                                                                                                                                                                                tGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGAt                                       >  1:412358/1‑250 (MQ=255)
                                                                                                                                                                                     gAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                 >  2:287389/1‑140 (MQ=255)
                                                                                                                                                                                     gAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                 <  1:287389/140‑1 (MQ=255)
                                                                                                                                                                                                aaaTCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCACGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                  >  2:188420/1‑112 (MQ=255)
                                                                                                                                                                                                aaaTCAGGCTTGCCGAGAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAgcttgctt                                                                                                                                                                  <  1:188420/112‑1 (MQ=255)
                                                                                                                                                                                                                       ccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCg  >  1:187671/1‑251 (MQ=255)
                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                                         
ACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCG  >  NC_000913/2173149‑2173613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑