Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,373	C→T	T103T (ACC→ACT)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,373	0	C	T	100.0%	146.9 / NA	46	T103T (ACC→ACT)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/19);  total (27/19)

CGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGC  >  NC_000913/4396132‑4396615                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    cgACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGt                                                                                                                                                                                                                                            >  2:229317/1‑251 (MQ=255)           tGATATTCAGGTTTCTACCGGTAATCAACAGGCGCGGATAACGTTGAGGTTTATTGGCGATCCTGAATATGCGTTTAGCCATCAAAGCAAACGCACCGGGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGTTGGTCGATCTTACTGAAAACGGTAAAACCGaa                                                                                                                                                                                                                                   <  2:217828/250‑1 (MQ=255)                  cAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGa                                                                                                                                                                                                                            >  2:102117/1‑250 (MQ=255)                   aGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTAGTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAg                                                                                                                                                                                                                          <  2:226491/251‑1 (MQ=255)                       ttCTAACGGTCATCAACAGGCGCGGATAACGCTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGGGGTCGGTCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGc                                                                                                                                                                                                                      >  2:119379/1‑251 (MQ=255)                        tCTAACGGTAATCACAAAGCGAGGATCGCGGTGAGTTTTCTTGGCGATCATGATTATCCGTTTAGCCATCAAAGCAAGCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTGGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTATGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAAAGGTAAAACCGAAGCggctgtgcggc                                                                                                                                                                                                                      >  2:190175/1‑241 (MQ=255)                           aaCGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGTCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCa                                                                                                                                                                                                                     <  2:255384/248‑1 (MQ=255)                           aaCGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCa                                                                                                                                                                                                                     <  1:110447/248‑1 (MQ=255)                           aaCGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCa                                                                                                                                                                                                                     <  1:267521/248‑1 (MQ=255)                            aCGGTAAACAAAAGGCGCGGATAACGTTGAGATTTATTGGCGATCCTGACTATGGCTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTCAAGCTGCACAAACGCTACGGCTGGTGGTCGCTCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGgaagaa                                                                                                                                                                                                                  >  2:238347/1‑250 (MQ=255)                                    cAACAGGCGCGGATAACGTTGAGTTTTATTTGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCaa                                                                                                                                                                                                         <  1:422/251‑1 (MQ=255)                                         ggCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTac                                                                                                                                                                                                     >  2:90765/1‑250 (MQ=255)                                               gATAACGGTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTgtcg                                                                                                                                                                                              <  2:223863/251‑1 (MQ=255)                                                aTAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTgtcgt                                                                                                                                                                                             >  2:91106/1‑251 (MQ=255)                                                             ttATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAAc                                                                                                                                                                                >  1:86218/1‑251 (MQ=255)                                                                   gCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTGACGATTAACGCCGAt                                                                                                                                                                          <  2:272275/251‑1 (MQ=255)                                                                     gATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAACACGAAGCGGGGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGAtgg                                                                                                                                                                        >  2:258648/1‑250 (MQ=255)                                                                      aTCCTGATTATGAGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGCCTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGAGGCAGAATGGCAGCAATTAAAATGTCGTCTTTACGATTAACGCCGAtgtg                                                                                                                                                                       >  2:145556/1‑251 (MQ=255)                                                                             ttATGCGTTTAGACATCAAAGCAAACGCACCGTGGCGATCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGGGAAGCGGCAGAAGGGCAGCAATTACACTAGCGTCTTTACGATTAACGCCGA‑GTGGCCGCCAc                                                                                                                                                               >  2:68955/1‑251 (MQ=255)                                                                              tATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAACGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTAAACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCAcc                                                                                                                                                              >  2:208869/1‑251 (MQ=255)                                                                                  cGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGcct                                                                                                                                                          >  1:236309/1‑251 (MQ=255)                                                                                     ttagcaatcacaggacaCGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTCAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCACTTACACTGTAGTCTTTACGATTAACGCCGAGTTG‑CCGCCACCGactcct                                                                                                                                                       >  2:189355/17‑251 (MQ=255)                                                                                           aTCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTccgccg                                                                                                                                                 >  1:155094/1‑251 (MQ=255)                                                                                             cAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGTAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCgccgcc                                                                                                                                               >  1:28581/1‑251 (MQ=255)                                                                                                                            aacaaacGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCACCCGTGGTTGCGAAACGCGTTGAAACGCCTGCg                                                                                                                >  1:227953/1‑251 (MQ=255)                                                                                                                                aacGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTGGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CAGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTg                                                                                                            >  1:88046/1‑251 (MQ=255)                                                                                                                                  cGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTc                                                                                                          >  2:165452/1‑251 (MQ=255)                                                                                                                                  cGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGAGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTc                                                                                                          <  1:54226/251‑1 (MQ=255)                                                                                                                                  cGGGCGTGATTCAGGGACTGCCGTGGTTGTTCAGCGGCAATAAGCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCATCAATTACTCTGGCGTCTTTCCGATTAACGCCGATTTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTc                                                                                                          <  2:229085/251‑1 (MQ=255)                                                                                                                                       gTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCTGTTGGCGCAcc                                                                                                     <  2:43227/251‑1 (MQ=255)                                                                                                                                        tGATTCAGGGACTGCCGTTGTTGTTCAGCGGCACTAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCAGCCCATGGTTGCGAAACGCGTTGAAACTCATGCGGTTGTCGCACcg                                                                                                    >  1:65007/1‑251 (MQ=255)                                                                                                                                                    ggaggtttgtgttTAGCGGCAATAATCTGGTGAAGGGGGTTCGCGCTGGAACGCCTAATTATTCACAAACGCTTCGGCGGGTGGTGGATCTTCCTGAAAACGTTAACACCGAAGCGGTAAAGCGGTAGAGTTGCAGCAATTACTCTGTCGTCTATCCGATTAACGCCGATGTG‑CCGCCTCCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGTGATGAAACTCTTGCCGTTTTCGCACTGCGCGTCAGccaa                                                                                        <  2:273267/242‑4 (MQ=255)                                                                                                                                                      ccGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAAcc                                                                                      <  1:229317/251‑1 (MQ=255)                                                                                                                                                      ccGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCGAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAAGTACACTGGCGGCTTTACGATTAACGCCGATGTG‑CCGCCGCCGCCTCCTCCGCCGCCCTTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCTCCGCGCGTCAGCGAAcc                                                                                      <  2:190169/251‑1 (MQ=255)                                                                                                                                                                      gCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGttt                                                                      >  1:869/1‑251 (MQ=255)                                                                                                                                                                           aatCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCCCAACCGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACAGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAAc                                                                 >  1:239001/1‑251 (MQ=255)                                                                                                                                                                                ggTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGaa                                                             <  1:6034/250‑1 (MQ=255)                                                                                                                                                                                        cGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGc                                                    >  1:101238/1‑251 (MQ=255)                                                                                                                                                                                            tCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACt                                                 <  1:240634/250‑1 (MQ=255)                                                                                                                                                                                                                    acaAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACgg                        <  1:102117/251‑1 (MQ=255)                                                                                                                                                                                                                         cGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAAcg                   <  2:876/251‑1 (MQ=255)                                                                                                                                                                                                                            tACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGACAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGt                >  2:221484/1‑251 (MQ=255)                                                                                                                                                                                                                             aCGGCTTGTGTTCGATCTTCCTGAAACCGGTAAAACCGAAGCTGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATTTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAATCGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAAcgcg                 <  1:181656/249‑1 (MQ=255)                                                                                                                                                                                                                              cGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTcc              >  1:39411/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggtggtCGATATTACTGAAAACGGTAAAACCGAAGCGATGAAGCGGCAGAATGGCAGCACTCACACTGTCATCTTTCCGATTAACGCCGATGAG‑CCGCCACCGCCTCCTCCGCCGCCCCGGGTTGCCAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCCATCCCTTTAAAACGGAAAGGAACACCATTACGGGAGTTTTAAGCAGACATATGGTAACGAGTcccgc           >  2:264974/1‑246 (MQ=255)                                                                                                                                                                                                                                          gATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAgcgcgcgc  >  2:84100/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    CGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTG‑CCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGC  >  NC_000913/4396132‑4396615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑