Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,602	A→C	Y101D (TAT→GAT)	actP ←	acetate/glycolate:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,602	0	A	C	100.0%	120.6 / NA	34	Y101D (TAT→GAT)	actP	acetate/glycolate:cation symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/17);  total (17/17)

GATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATA  >  NC_000913/4284359‑4284840                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               gATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACAGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAg                                                                                                                                                                                                                                         <  2:194439/251‑1 (MQ=255)          ggCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACAcc                                                                                                                                                                                                                                >  1:246693/1‑251 (MQ=255)              aaaCAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAgcg                                                                                                                                                                                                                            <  1:273619/251‑1 (MQ=255)                    cagcTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGGAAACACCAGCGCgg                                                                                                                                                                                                                         >  2:177865/1‑248 (MQ=255)                        tCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATccc                                                                                                                                                                                                                  >  2:6150/1‑251 (MQ=255)                               gTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAAt                                                                                                                                                                                                           <  2:134585/251‑1 (MQ=255)                               gTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGCGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAACTGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACCCCAGCGCGGAGATCCCCAAGAAt                                                                                                                                                                                                           >  1:153061/1‑251 (MQ=255)                                tttACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCACCCACCAACAGAGAACCACAGGCCGAAAGAATACGACTCGGCCCTTGTTTCAGACGGTAAGAGGCCACCTCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATg                                                                                                                                                                                                          >  1:25907/1‑251 (MQ=255)                                                                              accaGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGaa                                                                                                                                                            <  1:88471/251‑1 (MQ=255)                                                                                     aaCCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAAGTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGTAGATGCCCAAGAATGAGGCGGCGGACATATGGGCCCGGGCAAGCTCCAGCCGGTTCTGGAAGCCAGTg                                                                                                                                                     <  2:271814/251‑1 (MQ=255)                                                                                                  aaGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGACCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTACACCGTCATCGCCGGAGGTAAACACAACCGCGGAGATCCACAAGAATGAGGCGGCGGACATATAGGACCCGGCAACCGCCAGCCCGCTCTGGAAGCCAGTGATATTGCCGCCTg                                                                                                                                        >  1:153153/1‑251 (MQ=255)                                                                                                   aGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGc                                                                                                                                       >  1:159984/1‑251 (MQ=255)                                                                                                    aaaTACGAAGCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGAGCGTCCAGCCCACCAGGAAGCCCGGCGTGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGATGCGGCTGACAGATAGGCCCCGGCAATTGCCAGCCCGTTCTGGAAGCTGGTGATATTGCCGCCTGCg                                                                                                                                      <  2:30544/250‑1 (MQ=255)                                                                                                          gAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTgtag                                                                                                                                <  1:266880/251‑1 (MQ=255)                                                                                                               ggCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTTTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTc                                                                                                                           <  2:280064/251‑1 (MQ=255)                                                                                                                                                   aaGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGTTTGAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGt                                                                                       <  1:28968/251‑1 (MQ=255)                                                                                                                                                       tgtagcgcccacggtaaagaaGACGTTAGGAAATAAGCAACAAAATGATCGACCAGCCCACCAGGCAGCCCAGCGAGCAAATTAAGCCGTCATCGCAGGAGCAAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCAGGAAATCGCCAGCCCGTTCTGGACGCCAGTGATATTGCCGCCTGCGGTGTAGTCGTCGTTACGTGAACGAAAGCGTTTTGATGACTAGTAGGTTATg                                                                                   >  2:120485/21‑251 (MQ=255)                                                                                                                                                       ggtAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATg                                                                                   <  2:226607/250‑1 (MQ=255)                                                                                                                                                             gcCCCAGGGTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGc                                                                             <  2:36977/251‑1 (MQ=255)                                                                                                                                                                    gTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAac                                                                       >  2:9998/1‑250 (MQ=255)                                                                                                                                                                       aCGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACacga                                                                   <  2:173547/251‑1 (MQ=255)                                                                                                                                                                            gACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaa                                                               >  1:56425/1‑250 (MQ=255)                                                                                                                                                                            gACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGaa                                                               >  1:38408/1‑250 (MQ=255)                                                                                                                                                                                           ggAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATTGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACataat                                                >  2:197922/1‑250 (MQ=255)                                                                                                                                                                                             aaCAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATaataat                                             >  1:40644/1‑251 (MQ=255)                                                                                                                                                                                                  aaTGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAACTTAAGCCGTCATCGCCGGAGGAAAACACAAGCGCGGAGATCACCAAGACTGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGACGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAAAACGACGAAAATCAGGCACATAATAATAGCCt                                        >  1:248840/1‑251 (MQ=255)                                                                                                                                                                                                           ccAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCg                               <  1:265379/251‑1 (MQ=255)                                                                                                                                                                                                                         aGCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGctct                 <  1:176223/251‑1 (MQ=255)                                                                                                                                                                                                                          gCCCAGCGAGTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGctct                 >  1:10319/1‑250 (MQ=255)                                                                                                                                                                                                                                   gTAAATTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGc       >  1:120447/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaaTTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCt      <  1:6150/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaaTTAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTa     <  2:211118/251‑1 (MQ=255)                                                                                                                                                                                                                                        ttAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAAt   >  2:257119/1‑250 (MQ=255)                                                                                                                                                                                                                                         tAAGCCGTCATCGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATa  <  1:105606/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               GATAGTTAAGGCCAAACAGCAGCTCGATCAGTTTACCTGCGCCCACCATCTGGGCGATAAGGTAAAGCGCCACCACCACCAGAGAACCACAGGCCGAAAGAATACGAATCGGCCCTTGTTTCAGACGGTAAGAGGCCACATCGGCAAAGGTGTAGCGCCCCAGGTTACGCAGACGTTCGGCAATCAGGAACAAAATGATCGGCCAGCCCACCAGGAAGCCCAGCGAGTAAATTAAGCCGTCATAGCCGGAGGTAAACACCAGCGCGGAGATCCCCAAGAATGAGGCGGCGGACATATAGTCCCCGGCAATCGCCAGCCCGTTCTGGAAGCCAGTGATATTGCCGCCTGCGGTGTAGTAGTCGCTACGAGAACGTACGCGTTTTGATGCCCAGTAGGTAATGCCGAGCGTAAACACGACGAAAATCAGGAACATAATAATCGCCTGCCAGTTCGTTGGCTGGCGCTCTACGGCCCCGCTAATA  >  NC_000913/4284359‑4284840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑