Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,703,531	G→T	Y106* (TAC→TAA)	dppD ←	dipeptide ABC transporter ATP binding subunit DppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,703,531	0	G	T	100.0%	107.5 / NA	33	Y106* (TAC→TAA)	dppD	dipeptide ABC transporter ATP binding subunit DppD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/18);  total (15/18)

GGTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTGTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGC  >  NC_000913/3703288‑3703775                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ggTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACg                                                                                                                                                                                                                                               <  2:63221/251‑1 (MQ=255)   tCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCCTAATCTGGAAAACCACGGTTTAGGACggg                                                                                                                                                                                                                                             >  1:190283/1‑251 (MQ=255)    cACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACgggg                                                                                                                                                                                                                                            >  1:231413/1‑250 (MQ=255)      cGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTa                                                                                                                                                                                                                                          <  1:23237/251‑1 (MQ=255)       gTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTaa                                                                                                                                                                                                                                         <  2:149892/251‑1 (MQ=255)       gTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAAGCAATCGCCATGGCGCTCATCACGCGCTAGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGCATTTTGTTGCACCCCTGATGCACCTTAATCTCCTCCATAATCTTGAAAACCAAGGTTTAACACGCGTTa                                                                                                                                                                                                                                          >  1:161647/1‑250 (MQ=255)                              cGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAg                                                                                                                                                                                                                  <  1:211505/251‑1 (MQ=255)                                          gTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCTCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGTTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACt                                                                                                                                                                                                      <  1:266306/251‑1 (MQ=255)                                               ggCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCgg                                                                                                                                                                                                  <  2:225244/250‑1 (MQ=255)                                                  cGACAGGCAATCGCCACGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTTTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTTGAAGATCATCGCCACTTCGGcacc                                                                                                                                                                                              >  1:52668/1‑251 (MQ=255)                                                       ggCAATCGCCATGGCGATCATCCCGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACccacca                                                                                                                                                                                         <  2:5339/251‑1 (MQ=255)                                                             cGCCATGGCGAGCATCACGCGCGGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGGCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCTCCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTg                                                                                                                                                                                    <  2:204751/250‑1 (MQ=255)                                                                                 gcTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGAAGCACCTTAATCGCTTCCATAATCTGGAAACCCACCGTTTATCACGGGGTAAGGCTTGGCATCGGGTCCCGGAAGATCATCGCCACTTCGGCACCCACCCGGTTGGGGCGCTCTTTTTCttggat                                                                                                                                                                >  1:208224/1‑245 (MQ=255)                                                                                     gCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCCTAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGGAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTTTGAGATAAGCt                                                                                                                                                           >  1:218848/1‑251 (MQ=255)                                                                                              gccgGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCt                                                                                                                                                  <  2:16390/251‑1 (MQ=255)                                                                                                    aaGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGGACTTTTGTTGCCGCCCTGCTGCCCCTTAATCGCCCCCATAATCTGGAAAACCCCGGTTTTTCACGGGTTAAGGCTGGTCATCGGGGCCTGGAAGAACATCGCCCCTTCGGCCCCCCCCCGGGTGCGGCGCTCTTTTTCTGAGATACCCTGCAAATCCTGGCCgg                                                                                                                                            >  2:216407/1‑250 (MQ=255)                                                                                                         gATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTTATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTGTCTGAGATACGCTGCAAATCCTGGCCGTTaa                                                                                                                                         <  2:135054/249‑1 (MQ=255)                                                                                                                             cgcgATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTAc                                                                                                                   <  1:115828/251‑1 (MQ=255)                                                                                                                                       gATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGGTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCCGCCCCCACCCGGTTGCGGCGCCCTTTTTCTTAGATACGCTGCAAATCCTGGCCGTTAAAATCCAGGTTTTCTTCCATTACGCGGccccgg                                                                                                         >  2:218755/1‑248 (MQ=255)                                                                                                                                             gAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAAACCCTTCCCTAATCTGGAAACCCACGGGTTAGCACGGGGTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCCCCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAAACCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATaatc                                                                                                    >  1:168490/1‑250 (MQ=255)                                                                                                                                                             cagcagGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATc                                                                                    <  2:105917/250‑1 (MQ=255)                                                                                                                                                                              gctGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGAc                                                                  <  2:28700/251‑1 (MQ=255)                                                                                                                                                                                              tttGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACt                                                  >  2:156511/1‑251 (MQ=255)                                                                                                                                                                                                 gttgCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCa                                                <  2:190117/250‑1 (MQ=255)                                                                                                                                                                                                       gccCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCcaca                                          >  2:34818/1‑250 (MQ=255)                                                                                                                                                                                                         ccTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATc                                       <  2:226758/251‑1 (MQ=255)                                                                                                                                                                                                                        ccGCTTCCAAAATCGAGAAACACACGGTTGAGCACGGTTTAAGGCTGGTCGTCGGGTCGTGGAAGATCATCGCCACTTGGGCCCCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCTCCCACATACCCTACCCCTTCGccc                        <  2:274578/250‑1 (MQ=255)                                                                                                                                                                                                                          gCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTAc                                                                                                                   >  2:208054/1‑158 (MQ=255)                                                                                                                                                                                                                          gCTTCCATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTAc                                                                                                                   <  1:208054/158‑1 (MQ=255)                                                                                                                                                                                                                               cATAATCTGGAAACCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGtttt                  >  2:174061/1‑250 (MQ=255)                                                                                                                                                                                                                                       ggAAACCCACGGTTTAGCACGGGTTAAGGCTGGTTATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGACCAATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATACCGACCACTTCGCCCTGTTTTACGCTGt           >  1:256/1‑249 (MQ=255)                                                                                                                                                                                                                                         aaaCCCACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCt       <  1:174061/251‑1 (MQ=255)                                                                                                                                                                                                                                              cACGGTTTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGc  >  2:127601/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     GGTCACGTCCAGCGCGGTGGTCGGTTCATCGGCAATCAGCAGTTTTGGCCGACAGGCAATCGCCATGGCGATCATCACGCGCTGGCTCATGCCGCCGGAAAGCTGATGCGGGTAAACATCCAGACGCGATGCCGGATCGGGAATACCGACCTGATTCAGCAGGTCAATCGCTCGCTGACGGCGGGTACTTTTGTTGCCGCCCTGATGCACCTTAATCGCTTCCATAATCTGGAAACCCACGGTGTAGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGC  >  NC_000913/3703288‑3703775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑