Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,867,455	G→A	Q33* (CAG→TAG)	rpoS ←	RNA polymerase sigma factor RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,867,455	0	G	A	100.0%	145.1 / NA	40	Q33* (CAG→TAG)	rpoS	RNA polymerase sigma factor RpoS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/21);  total (19/21)

GCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCG  >  NC_000913/2867208‑2867698                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     gCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTc                                                                                                                                                                                                                                                   <  1:214583/251‑1 (MQ=255)     gACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTctact                                                                                                                                                                                                                                               <  2:117527/251‑1 (MQ=255)                       ggcggGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCaaaa                                                                                                                                                                                                                             <  2:111464/251‑1 (MQ=255)                                               gCAAGTTACTCTCGATCATCCGGCGGCGAGATGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCGTCGGCCGTTAACAGTGGTTAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACTCGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCAC‑GGGTGTCCTATTCGACTAAGGCCTGTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAt                                                                                                                                                                                                     <  1:114330/251‑1 (MQ=255)                                                 aaGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAATGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc                                                                                                                                                                                                   <  1:265053/251‑1 (MQ=255)                                                 aaGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc                                                                                                                                                                                                   <  2:234109/251‑1 (MQ=255)                                                           cGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCtt                                                                                                                                                                                           <  1:192329/249‑1 (MQ=255)                                                           cGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCt                                                                                                                                                                                            <  2:120317/248‑1 (MQ=255)                                                            gATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAt                                                                                                                                                                                        >  1:186871/1‑251 (MQ=255)                                                                atcCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGAATCTTCATTTaa                                                                                                                                                                                    <  1:53513/251‑1 (MQ=255)                                                                         cgggatgCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTGCTTCTGCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACTCGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAGCCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAAc                                                                                                                                                                           <  2:257504/245‑1 (MQ=255)                                                                         cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAAc                                                                                                                                                                           >  1:245747/1‑251 (MQ=255)                                                                                    atatcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCTTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTAt                                                                                                                                                               >  2:218341/4‑251 (MQ=255)                                                                                    aTCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAAcgtt                                                                                                                                                                        <  2:174586/243‑4 (MQ=255)                                                                                    aTCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACttt                                                                                                                                                                        >  1:174586/1‑243 (MQ=255)                                                                                                               aTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCg                                                                                                                                    <  1:131653/251‑1 (MQ=255)                                                                                                               aTAAACTTCTTCTTCGGCCGTTAACAGCGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCg                                                                                                                                    >  1:80170/1‑251 (MQ=255)                                                                                                                   aCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAATGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTCTTCTGACTCATAAGGCGGCTCCTAccctcgat                                                                                                                                >  2:217406/1‑246 (MQ=255)                                                                                                                   aCTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCCAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCCTACGGTGGCTCCTACCCGTGAt                                                                                                                                >  2:69213/1‑251 (MQ=255)                                                                                                                            tcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTACCACTGGGT‑TCCTATTCACCTAAGCCCTTTCCGTCAAAACCCTCAACTCCGTTCTCACCAAATTCGGCATTTTCATTTAAATATTGAA‑TTTCAGGCGTATTCTGACTCTTAAGGGGGCTCCTACCCGTGATCCCTTGACg                                                                                                                       >  1:93859/1‑251 (MQ=255)                                                                                                                                 cGTTAACAGTGGTGAATAACCAATCTCACCAAGCCAAAGCTGAGACCCATACAACACACGCTGTGTGGCTCCCTGCGATACCAGTTCCACTTCGGCCACATAGTTATCACTGGAC‑TCCTAATCTACTAAGGCCTTTCCGTCAAAAACCTCAACTCCATTCTCATCACATTACGCATCTCCATCTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAAc                                                                                                                   >  1:130350/1‑250 (MQ=255)                                                                                                                                  gTTAACAGCGCTGAACAACCAATGTCACCAAGGCAAAGCAGAGTCGCGTCAACCACCCACTGTGTGCCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGACCTTTCAG‑CGTCTTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACAt                                                                                                                 >  2:36382/1‑251 (MQ=255)                                                                                                                                     aaCAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCa                                                                                                              <  2:236798/251‑1 (MQ=255)                                                                                                                                         gtggtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCa                                                                                                          <  1:64900/251‑1 (MQ=255)                                                                                                                                                  aaCCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTg                                                                                                  <  2:245747/250‑1 (MQ=255)                                                                                                                                                               aaGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCTTTGACGGAACATTCAAGCAAAAGCCTGGTGCCGCCGATTTa                                                                                    <  2:21823/251‑1 (MQ=255)                                                                                                                                                                        cTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCATATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGCCGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCgg                                                                           >  1:259515/1‑251 (MQ=255)                                                                                                                                                                         tGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAACTCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGc                                                                          >  1:172584/1‑251 (MQ=255)                                                                                                                                                                                    catcactcGCTGTGTGGCTCCCTGCGATAACAGTGCCTCTTCGGCCAAATCATTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATGCTGACTCATAATGTGTCTCCGACCCGTGGTCCCTTGTCGGAACAGTCAAGCAAAAGCCTGGTTCCACCGATGTATCGCTGCGGCAAACAACggag                                                               <  2:171801/244‑4 (MQ=255)                                                                                                                                                                                       cacacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCgg                                                            <  2:172584/251‑1 (MQ=255)                                                                                                                                                                                             cTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTAc                                                      >  2:8802/1‑251 (MQ=255)                                                                                                                                                                                                            cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGt                                        >  2:37009/1‑250 (MQ=255)                                                                                                                                                                                                                  cAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAAt                                 >  1:222253/1‑251 (MQ=255)                                                                                                                                                                                                                       ccTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCa                             >  2:108742/1‑250 (MQ=255)                                                                                                                                                                                                                       ccTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCCCAACTCCGTTCTCATCAAATTCCGCACCTTCATTTAAATCATGATCTTTCAG‑CGTATTCTGACGCATAAGGTGGCTCCTACCCGTGATCCCTTGGCGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGaaatgcaa                            <  2:266024/251‑7 (MQ=255)                                                                                                                                                                                                                               gCCAAATCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAg                    <  2:93859/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaTCGTTATCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAATGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCgtg                >  1:39540/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTCACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACt         >  1:111818/1‑251 (MQ=255)                                                                                                                                                                                                                                            cACTGGGT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTg        <  1:108742/250‑1 (MQ=255)                                                                                                                                                                                                                                                 ggT‑TCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCg  >  2:182100/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     GCCAGACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGT‑TCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAG‑CGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTCCG  >  NC_000913/2867208‑2867698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑