Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,414,829	A→G	E28G (GAA→GGA)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,414,829	0	A	G	100.0%	100.1 / NA	33	E28G (GAA→GGA)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/21);  total (12/21)

CCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTT  >  NC_000913/2414599‑2415061                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         cccttccggcgttgtttATCCCGACCAACGAAGGCCGTGTTATCGCCCCATCCGCGACCCGCCTGGCTACCTGATTTCCCACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAGCGAAAGAAGATAAACCGTGTCCCGTAGTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCTTGATCCGTGCAATGGGACCCCAAAGCGTTCGTCTGa                                                                                                                                                                                                                      <  2:49718/236‑1 (MQ=255)  ccGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAg                                                                                                                                                                                                                     <  1:148545/251‑1 (MQ=255)   cGTCCTGCGGTGGTTAGCCCAACCAACGAAGAACTGTTTATCGCGCAAGACGCGAGCCGCCTTACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTAGGCTGATCCCTACCTGAACCAGCGTCTGTCTGACCGGCTTCAGCCTTGGCGGGATCCGGGCAATGGGACGCAAAGGCGTTCGTCTGAGc                                                                                                                                                                                                                    <  1:129649/251‑1 (MQ=255)    gTCCTGGGTTGGTTAGCCCAACCTACGAAGACCTGGTTATCGCGCAAGAGGCTGGCGGCCTTACTGCCTGATTGCACACCGCCAGCTCAGCTGGCGGGGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCATGATCCGTGCAATGGGACGCaaatgctttctgctgagcc                                                                                                                                                                                                                   <  2:102926/251‑17 (MQ=255)     tCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAAAGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGGCGGTCTGACCAGCGGCAGCCTTGGCGGGATCCGTGCCATGGGACGGCAAGGCGGTCGTCttggggg                                                                                                                                                                                                                  >  2:3850/1‑245 (MQ=255)      ccTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGCAAGAGGGTAAAACGTTTCCCCACTTATTATGCTGATCCCTACCGGAAACAGCCTCGGTCTGACCAGCGTCCGCCTTGGCGTGATCCGCGACATGGGACGCCAAGGCGTTAGTCTGAGCGtt                                                                                                                                                                                                                 >  2:257981/1‑251 (MQ=255)          cGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGACTGACTGCCTGATTTCACACCGCCCGATCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCCTGTCCCGTATTATTATGCTGATCCATACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGAGCACTGGGACGCAAAGGCGTTCTTCTGAGCGTTTTc                                                                                                                                                                                                              >  1:17574/1‑250 (MQ=255)                         ccaacGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGc                                                                                                                                                                                              >  1:195600/1‑251 (MQ=255)                           aacGAAGAACTGGTTATCGCGAAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCcg                                                                                                                                                                                            <  1:151524/251‑1 (MQ=255)                               aagaaCTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCAGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTa                                                                                                                                                                                        <  1:177923/251‑1 (MQ=255)                                agaaCTGTTTATCGCGCAAGACGCGAGCCGCTTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTAc                                                                                                                                                                                       <  1:191008/251‑1 (MQ=255)                                             gcgcAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGCGTCCCGTATTATTATGCTGACCCCTACCGGAACCACCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAAGCGTTCGTCTGAGCGTTTTCAAAACTATCGCTCAGCCGCGTACCGGTGGCGATgcg                                                                                                                                                                          >  2:205979/1‑251 (MQ=255)                                                                  aCTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATc                                                                                                                                                     <  2:58543/251‑1 (MQ=255)                                                                                           cagctGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCacg                                                                                                                             <  2:118888/250‑1 (MQ=255)                                                                                                ggCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGcc                                                                                                                       <  1:163591/251‑1 (MQ=255)                                                                                                         gTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGccgctgaacc                                                                                                               <  1:225898/250‑1 (MQ=255)                                                                                                                 aCCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAAt                                                                                                      <  1:3850/251‑1 (MQ=255)                                                                                                                    cGCCAAATCGGCGGTAACCAAAGAGGAGAAACCGTGGCCCGTATTATTATGCTGAACCCTACCGGAATCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTAGCGCTCAGCCGCGTACCGGTGTCGATGCGCCCGATCAGACTACTACTATCGTAAGTGCGAACTCTTCCACCACGACGGCGGCTCATCCTCTGAGAATGAg                                                                                                   <  2:25570/251‑1 (MQ=255)                                                                                                                     gCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGACCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGc                                                                                                  <  1:205979/251‑1 (MQ=255)                                                                                                                                                        tCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCAACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCCGaa                                                                  >  2:26619/1‑248 (MQ=255)                                                                                                                                                           cgatcgtccgatcTGATCACGACCGGAACCAGCGGCGGTCTGACGAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGGGCGTCTGAGAGGTTTCAAACCTATCGCTCAGGCGCGTACCGGTGGGGATGCGCCCGATCAGACGACGACTAGCGTGCGTGGGAAGTCGTACACCACGACGGCCTCTGAACCGAGGAAAATGAGAGACGGTGAAGGTCTGCAGTCCAGCAATCAGAAAGAtgt                                                            <  2:133781/239‑1 (MQ=255)                                                                                                                                                            gTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGAtgtg                                                           <  2:263704/251‑1 (MQ=255)                                                                                                                                                                   tatGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGCACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCATAAAGATGTGCTGATgg                                                    >  1:157419/1‑251 (MQ=255)                                                                                                                                                                       cTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGAt                                                                                                                                                                             >  1:225435/1‑126 (MQ=255)                                                                                                                                                                       cTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGAt                                                                                                                                                                             <  2:225435/126‑1 (MQ=255)                                                                                                                                                                       cTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGa                                                   >  1:45033/1‑248 (MQ=255)                                                                                                                                                                         gATCCCTACCGGAACCAGCGTCGGGCTGACCAGCGTCTGCCTTTGCGTGATCCGTGCCATGGGACGCAATGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGCTTGCGCTGCGCCCGATCACACTACTACTATCTTGCGTTCGATCTCTTCCCCCACGACCGCCGCTGCACCGCTGACACTGAGCTACGTTTAAGCTCTGCTTTCCCGCAATCacacagctgtagatcggtagagcc                                              >  1:133781/1‑228 (MQ=255)                                                                                                                                                                                 ccGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCa                                      <  1:222197/251‑1 (MQ=255)                                                                                                                                                                                    gAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCaaa                                    <  2:202459/250‑1 (MQ=255)                                                                                                                                                                                       ccAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGaagatcggaagagcacagtctga                                 >  1:185567/1‑228 (MQ=255)                                                                                                                                                                                       ccAGCGGCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGa                                                       <  2:185567/228‑1 (MQ=255)                                                                                                                                                                                              cGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCt                          >  1:29174/1‑250 (MQ=255)                                                                                                                                                                                                                     gCGTGATCCGTGCAATGGGACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGtt  <  1:141046/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         CCCGTCCTGCGGTGGTTATCCCAACCAACGAAGAACTGGTTATCGCGCAAGACGCGAGCCGCCTGACTGCCTGATTTCACACCGCCAGCTCAGCTGGCGGTGCTGTTTTGTAACCCGCCAAATCGGCGGTAACGAAAGAGGATAAACCGTGTCCCGTATTATTATGCTGATCCCTACCGGAACCAGCGTCGGTCTGACCAGCGTCAGCCTTGGCGTGATCCGTGCAATGGAACGCAAAGGCGTTCGTCTGAGCGTTTTCAAACCTATCGCTCAGCCGCGTACCGGTGGCGATGCGCCCGATCAGACTACGACTATCGTGCGTGCGAACTCTTCCACCACGACGGCCGCTGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTT  >  NC_000913/2414599‑2415061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑