Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,948,964	G→A	A520V (GCT→GTT)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,948,964	0	G	A	100.0%	168.4 / NA	48	A520V (GCT→GTT)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/26);  total (22/26)

CGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAC  >  NC_000913/1948723‑1949204                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 cgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGGGTACCGTATTTCAGAACGTCGAGc                                                                                                                                                                                                                                          >  2:229736/1‑250 (MQ=255) cgAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGc                                                                                                                                                                                                                                          >  1:163772/1‑250 (MQ=255) cgAATGATTGCGTGTAGTTGAGTCATATCAGTTATTCTCAGCCTTCTTCGCAACCTGAATGCTCAGCTCAGCCAGTGCAGGCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTGTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAATACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCa                                                                                                                                                                                                                                         <  1:268035/251‑1 (MQ=255)       aTTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGc                                                                                                                                                                                                                                   <  1:215868/251‑1 (MQ=255)           cGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGaa                                                                                                                                                                                                                               <  1:54278/251‑1 (MQ=255)            gtgtATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAAt                                                                                                                                                                                                                              >  1:154751/1‑251 (MQ=255)            gtgtAGTTGAGTCATATCAGTTATTCTCAGCCTTCTTCAAAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCAGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAAt                                                                                                                                                                                                                              <  1:231624/251‑1 (MQ=255)                 tttGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTtctc                                                                                                                                                                                                                         >  1:98247/1‑251 (MQ=255)                   tGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTcg                                                                                                                                                                                                                        <  2:133677/250‑1 (MQ=255)                    gAGTCATATCAGTTATTCTCAGCCTTCTGCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTcgc                                                                                                                                                                                                                       <  2:264302/250‑1 (MQ=255)                           atCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTc                                                                                                                                                                                                                <  2:38382/250‑1 (MQ=255)                           atCAGTTATTCTCAGCCTTATTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGGCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTATCGCGCTGTTcc                                                                                                                                                                                                               >  1:76249/1‑251 (MQ=255)                              aGTTATTCTCAGCCTTCTTCACAACCTTAATGCTCAGCTCAGCCAGGGCAGTCGGGTTAGGAAAGCTCGGGGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCGAGACCTGCGTGCGGCGGAGTGCCGTATTTCAGAACTTCGAGCAGGAAGCCGAAGTTCTCGCGCTGTTTctct                                                                                                                                                                                                            <  2:35846/251‑1 (MQ=255)                              aGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCctct                                                                                                                                                                                                            >  1:91381/1‑251 (MQ=255)                                 tATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTc                                                                                                                                                                                                          <  1:215486/250‑1 (MQ=255)                                  aTTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCCGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGACTTTCTCGCGCTGTTCCTCTTCGt                                                                                                                                                                                                        >  2:189923/1‑251 (MQ=255)                                          ccTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATAccc                                                                                                                                                                                                >  1:88447/1‑251 (MQ=255)                                            ttcttcACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATAccc                                                                                                                                                                                                >  2:120514/1‑249 (MQ=255)                                                        tGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAacac                                                                                                                                                                                  >  1:50868/1‑251 (MQ=255)                                                            tGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCCGAATACCAAACACCGTc                                                                                                                                                                              >  2:149523/1‑251 (MQ=255)                                                             gctaagctcagcCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCAGCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTTTTCGTTGATACCCAGAATACCAAACTCCGTct                                                                                                                                                                             <  2:268321/247‑1 (MQ=255)                                                                cagctcagcCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTctgc                                                                                                                                                                           <  1:249023/250‑1 (MQ=255)                                                                         cAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCAc                                                                                                                                                                 >  1:137974/1‑251 (MQ=255)                                                                            tGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACCCGCTGCCGCCGTGGTTTTCGGGAAGGCGATCACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCACGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGCGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCAt                                                                                                                                                              >  1:277924/1‑251 (MQ=255)                                                                                         gCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTAc                                                                                                                                                 >  1:138817/1‑251 (MQ=255)                                                                                             aGCTCGGTGCTGCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGGTTATACCCAGAATACCAAACGCCGTCTGCTGCATATCACCATTATGGATACGTACTGaa                                                                                                                                             <  1:258797/251‑1 (MQ=255)                                                                                                 cGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAacc                                                                                                                                           <  1:63858/249‑1 (MQ=255)                                                                                                  ggTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAaccacc                                                                                                                                        <  2:67926/251‑1 (MQ=255)                                                                                                             catcaGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGt                                                                                                                             <  2:50868/251‑1 (MQ=255)                                                                                                                caGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAAc                                                                                                                          <  1:152176/251‑1 (MQ=255)                                                                                                                    cacGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCAtt                                                                                                                      <  2:98247/251‑1 (MQ=255)                                                                                                                              ccgTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCat                                                                                                             >  1:89005/1‑250 (MQ=255)                                                                                                                              ccgTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCat                                                                                                             >  2:214266/1‑250 (MQ=255)                                                                                                                                   gTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGt                                                                                                        >  2:109129/1‑250 (MQ=255)                                                                                                                                   gTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTa                                                                                                       <  2:89005/251‑1 (MQ=255)                                                                                                                                      ttCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAg                                                                                                     <  1:120514/250‑1 (MQ=255)                                                                                                                                         gggAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGCCGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACAACGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCCTATCGTAAGCGtt                                                                                                 >  2:37470/1‑251 (MQ=255)                                                                                                                                           gAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGTTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCg                                                                                               <  2:137974/251‑1 (MQ=255)                                                                                                                                           gAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCg                                                                                               >  1:109396/1‑251 (MQ=255)                                                                                                                                               gCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCAc                                                                                           <  2:98305/251‑1 (MQ=255)                                                                                                                                                cGATAACGTCACGGATATTCTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACAGAATGACAGACCTGCGTGCGGCGGAGTAACGAATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCCTATCACCATTATGGATACGTACTGAACCCCCGCCCACTTCGTAACCATTGATGACCATAACGTAAGCGTTCGCCAcc                                                                                          >  2:255905/1‑251 (MQ=255)                                                                                                                                                                       gTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTAGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATCTCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTc                                                                     >  2:95794/1‑249 (MQ=255)                                                                                                                                                                          ccGGTCAGCAGCATGGTCAGACTGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGCATGTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCTCCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCACTTc                                                                <  2:157105/251‑1 (MQ=255)                                                                                                                                                                                                   cAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtgag                                       <  1:56934/251‑1 (MQ=255)                                                                                                                                                                                                          gAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACg                                <  2:88447/251‑1 (MQ=255)                                                                                                                                                                                                                     cTGCGTGCGGCGGAGTACCGTATTTCAGAACGTCGCGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCACACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGCTGACCATCTCGTAACCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGACCGGATGGTGCAtt                     >  1:117163/1‑251 (MQ=255)                                                                                                                                                                                                                              gcggAGTACCGTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAgg            <  1:149523/251‑1 (MQ=255)                                                                                                                                                                                                                                        gTATTTCAGAACGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAc  <  2:109396/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CGAATGATTTCGTGTATTTGAGTCATATCAGTTATTCTCAGCCTTCTTCACAACCTGAATGCTCAGCTCAGCCAGTGCAGTCGGGTTAGCAAAGCTCGGTGCTTCAGTCATCAGACACGCTGCCGCCGTGGTTTTCGGGAAGGCGATAACGTCACGGATATTGTCGGTGCCGGTCAGCAGCATGGTCAGACGGTCAAGACCGAATGCCAGACCTGCGTGCGGCGGAGTACCGTATTTCAGAGCGTCGAGCAGGAAGCCGAATTTCTCGCGCTGTTCCTCTTCGTTGATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAC  >  NC_000913/1948723‑1949204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑