Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,829,887 G→A A293A (GCC→GCT)  astA ← arginine N‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,829,8870GA95.8% 71.3 / ‑5.8 24A293A (GCC→GCT) astAarginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (12/11);  minor base T (1/0);  total (13/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATC  >  NC_000913/1829642‑1830133
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       
gccttgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGTTCGGTTGCCGGATCGGTACGCCCCAGCACCACCCGGGAAATG‑TGATAATTTTCATTAGCGAc                                                                                                                                                                                                                                                    >  2:108488/1‑251 (MQ=255)
    tgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCCCCAGCACCACGCGG‑AAATGGGGATAATTTTCATTAGCGACcagg                                                                                                                                                                                                                                                >  1:173683/1‑251 (MQ=255)
    tgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACca                                                                                                                                                                                                                                                  <  2:40076/249‑1 (MQ=255)
                  gCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGGTCGGTACGCACCAGCACCACGCGG‑AAATGGGGATAATTTTCATTAGCGAACAGGCAGGCTGGGAAATc                                                                                                                                                                                                                                  >  1:35481/1‑251 (MQ=255)
                                   gCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGcc                                                                                                                                                                                                                 <  1:21716/251‑1 (MQ=255)
                                                           ccGTTATCCAGTAACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGACCGGTACGAACCAGCACCACGCGG‑AAATGGTGATAATTTTCCTTAGCGACCAGGCCGGATGGGAAATAGGCCTGCGCCGGCTGCCCTTCTGCCACTTccaccacacgac                                                                                                                                                                                         >  1:193135/1‑245 (MQ=255)
                                                                              aaTCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGCTCGGCACGCACCAGAAACACGCGG‑ACATGGGGATAATTTTCATTTGCGACCAGGCAGGCAGGGAAATCGCCCTGCGCAGACTGCCCTTCTGCCACTTCCACCAGGCCG‑CTTTTACGGATCGGGCGCAc                                                                                                                                                                     >  1:11288/1‑251 (MQ=255)
                                                                                                               cacaGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTcac                                                                                                                                                       <  2:203673/232‑1 (MQ=255)
                                                                                                               cacaGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATGTGAGGGCATCCAGTTGTGCGTCGGTTAAAATCAAACGCTCGGTGGGCGTATCTGTACGCCCCAGCACCACGCTG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGTGAAAGCGGCCTTGGCCGGCTGCCCGTCCGCCAGTTCCTCCAG‑CCGAGTTTGTCGGATGGCGCGCACGCGGTGTAGGTCTCACGCAAGCGTCTGCCCAcc                                                                                                                                    <  2:31045/251‑1 (MQ=255)
                                                                                                               cacaGGCGCACAAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCCG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTcac                                                                                                                                                       >  1:203673/1‑232 (MQ=255)
                                                                                                                 caGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGt                                                                                                                                  <  2:175938/251‑1 (MQ=255)
                                                                                                                      gcACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTGTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAg                                                                                                                             <  1:234953/251‑1 (MQ=255)
                                                                                                                                  cgcgATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGGGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGGCGatat                                                                                                                   >  1:66761/1‑249 (MQ=255)
                                                                                                                                                gggCATTTGAGGGCAGCCAGTTGTGCGGCGGTTAAAATCAAACGCGCGGTTGCCGTGTCGGTACGCACCAGCACCACGCGG‑AAATGGTGGTAATTTTCATTAGCGCCCAGGCAGGCTGGGAAAGCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACGCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGaaa                                                                                                   <  2:252993/250‑1 (MQ=255)
                                                                                                                                                                                     tCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTc                                                                                                                                                   >  2:69560/1‑166 (MQ=255)
                                                                                                                                                                                     tCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTc                                                                                                                                                   <  1:69560/166‑1 (MQ=255)
                                                                                                                                                                                             tCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTCACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCAACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                                      >  2:196304/1‑251 (MQ=255)
                                                                                                                                                                                                      ggATCGGTACGCACCAGCAACACGCGG‑AACTGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCCAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAcc                                             >  2:118185/1‑251 (MQ=255)
                                                                                                                                                                                                       gATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCg                                            >  2:211203/1‑251 (MQ=255)
                                                                                                                                                                                                                                     tGGTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaaa                >  1:54524/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gggTGATAATTTTCAGTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATaaaaa               <  2:38381/250‑1 (MQ=255)
                                                                                                                                                                                                                                       gTGATAATTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGt             >  1:211551/1‑251 (MQ=255)
                                                                                                                                                                                                                                            aaTTTTCATTAGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGta        <  2:35481/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  cATTAGCGACCAGGCAGGCTTTGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCTACTTTTACGGATGGCGCGCACGCGGTCTATGTCACACTCAAGCGTCGGCCCACCGTCAAAGAGGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCATCACCGCGCGTGCGGGCGTGGTTTGCGGAGATACCTGACCGATGACTTCCTGTGCTTCCTGGGATAAAAAGTGAGTATAGATc  <  1:262766/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       
GCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACGCACCAGCACCACGCGG‑AAATGGTGATAATTTTCATTGGCGACCAGGCAGGCTGGGAAATCGCCCTGCGCAGGCTGCCCTTCTGCCACTTCCACCAG‑CCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATC  >  NC_000913/1829642‑1830133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑