Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	229,552	C→T	S129F (TCC→TTC)	dkgB →	methylglyoxal reductase DkgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	229,552	0	C	T	100.0%	52.5 / NA	16	S129F (TCC→TTC)	dkgB	methylglyoxal reductase DkgB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/5);  total (11/5)

CAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAAC  >  NC_000913/229315‑229761                                                                                                                                                                                                                                               |                                                                                                                                                                                                                  cAGAAAGTGGCGTGCCACGTCATGAACTCTGAATCACCACTAAAAGCTGGAATGAAAATCGCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGGGTACCGATTATGTTGATCTGACGGTAATCCACTGGCCGGCACCAAACGATGAAGTGTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGTGGTGAAGCGTG‑AGATCGGTAATTTCAATTTCACGATc                                                                                                                                                                                                      <  2:18999/251‑1 (MQ=255)                        gAACTCTCCCTCACCTCTACAATCTCGATTGCAAATCTCAGCAAAGACACATTCATCCCAAGTCTGAAAGAGAGCCTGCCAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGACGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAACACCAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGACAAAGCGATtgc                                                                                                                                                                               >  2:272484/1‑251 (MQ=255)                          aCTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATtgctg                                                                                                                                                                             >  2:74893/1‑251 (MQ=255)                                                                gCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGa                                                                                                                                        >  1:273572/1‑250 (MQ=255)                                                                   aaGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGCGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAActc                                                                                                                                    >  2:170531/1‑251 (MQ=255)                                                                    aGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGC‑TGTAGATCGGTATTTTCAACTTCACGATCCCGTTGATTGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATGGAActct                                                                                                                                   <  2:255361/251‑1 (MQ=255)                                                                                                                      aTTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGc                                                                                 <  1:245017/251‑1 (MQ=255)                                                                                                                           gTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCGTAGCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCa                                                                            <  1:26522/251‑1 (MQ=255)                                                                                                                                        tAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCtat                                                               >  2:161261/1‑251 (MQ=255)                                                                                                                                                                      tctcTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGaa                                  >  1:74054/1‑250 (MQ=255)                                                                                                                                                                      tctcTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTAATTCCTATATGACGCTGGCGTATGGTAAGGCCCTGaa                                  >  2:149786/1‑250 (MQ=255)                                                                                                                                                                               aaGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGtt                        <  1:161261/251‑1 (MQ=255)                                                                                                                                                                                   gcttATGCAAGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACTCGTG‑CGATCGGTATTTTCAACTTCACGATCACGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATATGCAAAACCGTAAAGTGGTTGTCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGGCTGGTAAGGCCCTGAAAGATGAGGTTCTTg                    >  2:913/3‑251 (MQ=255)                                                                                                                                                                                        tGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGt               >  1:160168/1‑251 (MQ=255)                                                                                                                                                                                                    tgGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTTCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaa    >  1:264801/1‑250 (MQ=255)                                                                                                                                                                                                     ggAAGACAAAACACACGGGCTGACGCGAG‑AGATCGGTATTTTCAACTTCACGATCCCGATGATGGAAAAAGAGATTGCTGCTGTTGGTGATGAAAACATCGATACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAATGGTTGCCTGGGCTAACCAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTaacc  >  2:165850/1‑249 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                  CAGAAAGTGGCGTGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCAAGTCTGAAAGAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGTTGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTG‑AGATCGGTATTTCCAACTTCACGATCCCGTTGATGGAAAAAGCGATTGCTGCTGTTGGTGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAAAACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGCATCCATATTACTTCCTATATGACGCTGGCGTATGGTAAGGCCCTGAAAGATGAGGTTATTGCTCGTATCGCAGCTAAAC  >  NC_000913/229315‑229761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑