Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	52.3 / NA	21	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

GAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGC  >  NC_000913/696240‑696716                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                                                                                                                                     <  1:105467/250‑1 (MQ=255)  aaaCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGGCGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCt                                                                                                                                                                                                                                   >  1:133638/1‑251 (MQ=255)   aaCGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCGTTTTGAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTGCCGCtt                                                                                                                                                                                                                                  <  2:240414/251‑1 (MQ=255)               tGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                                                                                                                                       >  1:95344/1‑250 (MQ=255)                 aaaTATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAAc                                                                                                                                                                                                                    <  1:110615/251‑1 (MQ=255)                                          acaacaTTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTACGCGTc                                                                                                                                                                                           >  2:23214/1‑251 (MQ=255)                                                                     tAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTATTGAAGAAATTGGCTGGGGTACGAgg                                                                                                                                                                 >  1:43163/1‑250 (MQ=255)                                                                                       gCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGcc                                                                                                                                              <  1:217589/251‑1 (MQ=255)                                                                                           cGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAACTGGCTGGGGTACGAGGAttagaccgccggaatgacggc                                                                                                                                           >  2:51938/1‑231 (MQ=38)                                                                                                                         gtCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGAt                                                                                                            <  1:92336/251‑1 (MQ=255)                                                                                                                              cATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGTGCGCCCTTATATGGTAAGCGTCTTGAATAAAGTGTCTGGGGTTCGCAGATTCGACCCGGCGAATGCCGGAATAAGAATCCGGTGCCTTACCGCTAGGCGATCcccc                                                                                                       >  2:266273/1‑251 (MQ=25)                                                                                                                                  gCATTCGACAATGAATGAACGCAGAAAAGCAAAAAGAACGCCGAAGCGAGCTTTGTTAATTAGGCTGGGGTACGAGTACTCGCGCCTCGGAATGCCGAAACTTGCAACCGGTGCCTTACCGCTTGGCTATACGCTAACTGGGTGCACTTACGAGGAAAGCGGCTTGAATAAAATGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTACCTTACCGCTTGGCGATACCCcatca                                                                                                   <  2:231092/251‑4 (MQ=19)                                                                                                                                                   aaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCCTATTTAATGAGGCTGAGGTACGAGGACTCCAACATCGGCATGCCGGACTTAGAACACGCTGACTTACCGCTTGGCGATACCCTCACCGGGTCCAATTACCAGGTAACAGTCTTGAATCACTTGGCTGGGGTACGACGATTCGAACCTCGGAATGACGGAATCAGAATCCGGTGACTTACCGCTCGGCGATACCCCAACCCATTGGCTTTGAAttgg                                                                                  >  1:260768/1‑249 (MQ=255)                                                                                                                                                     cGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCACAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACGTCGGCATGCCGGAATCAGCATCCGGTGCCTTACCGCTTTGCAATCCACCAACAAACTGGGTTTTCATTTGcc                                                                                >  2:47948/1‑251 (MQ=37)                                                                                                                                                      gCAGAAAAGCAAAAAGGGCGCCGAAGCGAGGTTTTTTAATGTGGCTGGGGTACGAGGATTCGAGACGCGGAATGACGGAATTAGAAACCGGTGCCTTCCCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg                                                                               <  2:133638/251‑1 (MQ=37)                                                                                                                                                                 agaaggacgcctgaccgggctgttttAATGTAGCTGCGGTACGAGGATACGAACCTAGGAATGTCTAAATTAGACTCCGATGCCTTACCGCGTGGAGATACGCCAACTGGGTGCACTGACGAGGTAAGCGTGTGGTATAAATTGGCTGGGGTACGAGTATTCGAACCTCGGAATGCCGGAATCAGAATTCGGTGCCTGACCGCTTGGCGGTACCCTAACAAATTGGTTGTGAATTTGCCGAACTGATTCGa                                                                    <  2:113396/229‑1 (MQ=16)                                                                                                                                                                 aaaaGCACGCCGAAGCGAGATTGGTCAAAGAGGCTGGGGTAGGAGCACACGAGACTCTGAAGGCCGGAAGTAGAAACCGGTGCATTACCGCTTGGAGAAACGCCGAATGGGTGCAATTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTAGAATTTGCCGAACATATTCGa                                                                    <  2:95344/251‑1 (MQ=16)                                                                                                                                                                    aggtagcctaaGCGAGCTTTGTTAGAGTGGCTGCGGTACGAGCACTCGAACCTCGGAAGGCCAGAATTAGAATCCAGTGCCTTCCCGCTTGGCTATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGTATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACAGATTCGATAc                                                                 <  2:248733/242‑1 (MQ=25)                                                                                                                                                                        cgccggagcgagcgttgtaaattgggCTGGGGTAGGAGGATTTGAACCTCGGAATGACGAAAATAGAATCCGGTGCCTTACCGCTTGGAGATACCCCAACTGGGTGAACGTACAAGGTAAGCGTCTTGAATAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCTCTTGGCGATACCCCAACAAATTGGTTTTTAATTTGCCGAACATATTCGATACATTc                                                             <  2:176899/227‑1 (MQ=25)                                                                                                                                                                                                                                 cGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGAtgt    >  1:106438/1‑251 (MQ=255)                                                                                                                                                                                                                                   gAATTAGAATCCGGTGCCTTCCCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGc  <  2:66556/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGC  >  NC_000913/696240‑696716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑