Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	76.6 / NA	25	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/12);  total (13/12)

AGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCG  >  NC_000913/4636679‑4637154                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      aGCCGGAGGTGCCTGTAATGCGGATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCGGGCGGAGTTGGCGCGTCCCGATTGGCTCTCCTGGGACCCAACGCATGGGCGACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCcccc                                                                                                                                                                                                                                    <  1:158546/250‑1 (MQ=255)           gCCTGTAATGCGTGTCGGCATGCGGTTGTTGCTGTGCTATTTTTTACTGGTGGCTGTGGCAGCTTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTGATCGACTCCGCATCGTTGCTGGCGGAGCTGGCGCGTCGCGATTTGCTCTCTGGGTACCCAACGCAGTGGCAACTGGCGCAGGCGTGTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCa                                                                                                                                                                                                                         <  1:180302/251‑1 (MQ=255)                 aaTGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTGCGCGCCAATATCg                                                                                                                                                                                                                   <  1:223427/251‑1 (MQ=255)                  aTGCGGATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCgg                                                                                                                                                                                                                  <  1:227814/251‑1 (MQ=255)                      gTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGc                                                                                                                                                                                                              >  1:268046/1‑251 (MQ=255)                          cGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCAtt                                                                                                                                                                                                           <  2:189651/250‑1 (MQ=255)                                 cGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACCA‑AATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAgg                                                                                                                                                                                                   >  2:230826/1‑250 (MQ=255)                                    ttgttgCTGGGCTATTTTTTACTGGTGGCGGTTGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAAACA‑CCCCCGTTTCGCGCCAATATCGGGGGCATTaaaaaaagg                                                                                                                                                                                                  >  2:129485/1‑246 (MQ=255)                                                                ggggggcggCCTGGTTCGTACTGTCCATGTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAGCGGAGGGGACTTTGATCGACACCGCAACGGTGCCGGGGGAGCTGGCGCGTCCCGATTTGCTCTCTGGCGGCCCAACGCAGGGGCAACTGGCGCAGGCGTGTAATCAGCTACTA‑TATCCCCCGTTTCTCGCCAATATCGGTGGCATTAACAAAGTGCGCCATGAATATCaaacctatattttcgac                                                                                                                                                                    <  2:218485/243‑17 (MQ=255)                                                                            ggTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCa                                                                                                                                                          <  1:55721/249‑1 (MQ=255)                                                                                 gTACTGGCCATTTGTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGTGCAAAGTAtt                                                                                                                                                   <  2:231550/251‑1 (MQ=255)                                                                                        ccATTTTTGTCAAAGAAGTTAAACCGTGCGTGCTAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAt                                                                                                                                            <  1:10587/251‑1 (MQ=255)                                                                                                        agTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGTCAAATAAAGCCg                                                                                                                            >  2:149416/1‑251 (MQ=255)                                                                                                           tAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGtt                                                                                                                          <  2:164534/250‑1 (MQ=255)                                                                                                               ccGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCGCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGGGGCATTAACAAAGTCCGCAATGAATATCATGTCTATATGACCGATGCGCCGGGCAAAGTATTGTTCGATTCGGCAAATAAAACCATTGGAc                                                                                                                      >  2:208553/1‑250 (MQ=255)                                                                                                                 gggCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAgg                                                                                                                   >  1:83337/1‑251 (MQ=255)                                                                                                                                aaCGGAGGGGAAGGTGATCGAAACCGCAACGTTGCTGGCGGAGCTGGAGCGTCACGATTTGCTCTCAAGGGACACAACGAATGGGCAACTGGCGGCGGAGTTTAATCAGCTACAA‑CATACCCCGTTTTGCGCCAATATCGGTACAATTAACAAAGAGAGAAATGAACATCATGTCTATCTGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCgcgcggg                                                                                                     >  2:256925/1‑247 (MQ=255)                                                                                                                                         gACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCt                                                                                           >  1:270494/1‑251 (MQ=255)                                                                                                                                               gATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTa                                                                                      >  1:33077/1‑250 (MQ=255)                                                                                                                                                                  cTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATgg                                                                  >  2:60903/1‑251 (MQ=255)                                                                                                                                                                   gggCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGt                                                                 <  2:100386/250‑1 (MQ=255)                                                                                                                                                                                                    gggACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATccc                                >  1:210351/1‑251 (MQ=255)                                                                                                                                                                                                               cATGGGCAACTGGCGCAAGCGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCt                       <  1:100065/249‑1 (MQ=255)                                                                                                                                                                                                                                  cGTTTAATCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCg  >  1:56129/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAg                                                                                                                               >  1:52445/1‑120 (MQ=255)                                                                                                                                                                                                                                        aTCAGCTACAA‑CATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAg                                                                                                                               <  2:52445/120‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                      AGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAA‑CATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCG  >  NC_000913/4636679‑4637154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑