| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,549,037 | T→C | V77A (GTC→GCC) | fimH → | type 1 fimbriae D‑mannose specific adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,549,037 | 0 | T | C | 96.9% | 91.2 / ‑5.5 | 32 | V77A (GTC→GCC) | fimH | type 1 fimbriae D‑mannose specific adhesin |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (15/16); minor base A (1/0); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
TGTAATGAAACGAGTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACT > NC_000913/4548804‑4549279 | tGTAATGAAACGAGTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAAACTTGCGCCCGTCGTGGATGTGGGGCAAAACCTTGTCGTGGATCTTTTGACGC‑AAAACTTTTTCCATAACGATTTT‑TCGGAAAACCTTACAGACTATGCCACACTGCAACGAGGCt > 1:219508/1‑251 (MQ=255) tAATGAAACGATTAATTCCCCTTTTTGCGGTACTGCTGATGGGCGGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCGATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTc < 2:150595/250‑1 (MQ=255) tAATGAAACGAGTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCCTCGTGGATGTGGGGCAAAAACTGGGCGTGGGTCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑TCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCg > 1:189938/1‑251 (MQ=255) aaaaCGAGTTATTCCCCTGTTTGCTGTCCTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATCCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCtt < 2:92607/250‑1 (MQ=255) aGTTATTACACTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCCAAGTTTATGTAAACCTTTCGCCCGGCGGGAATGTGGGGCAAAACCTGGTCGGGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑TCGGAAAACATTAAAGAATATTCCACACttgaaacaggcgcgggctatagcg > 1:65237/1‑228 (MQ=255) aCCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCCATGTTTTTGTAAACCTTGCGCCCGGCGGGAATGTGGGGCAAAACCTGGTCGTGGGTCTTTCGACCC‑CAATCTTTTTCCATAACGATTAT‑TCGGAAACCATTACAGACTATGCCACACTGCAACGAAGCTCGGCTTTTGgcggcggggt > 1:182897/1‑247 (MQ=255) ggCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCCGCGCCCATTTTTATTTAAAACTCTCGCCCCCCGTGAATGTGGGGCAAAACCTGGGCGTGGATCTTTCGCCGC‑AAAACTTTTTCCCTAACGCTTATTCGGGAAACC‑TTAAAGACCATGCCCCACTGCAACCAGGCTCCGCTTATTGCGGGGTGTTATTTCATTTTTTCGGGGCCggta > 2:116931/1‑247 (MQ=255) ggCTGGTCGGAAAATGCCTGGTCATTCGCCTGAAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTaaa < 1:129430/251‑1 (MQ=255) ggTCGGAAAATGCCGGGTAATTCGCCTTAAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAAGCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAata < 1:184810/250‑1 (MQ=255) gTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGACCCAAAGATTTTTTAAAACCTGCGCCCGTCGTGGATGTGGGGGAAAACCTGGGTGGGGGTTTTTCGACGC‑AAATCTTTTTCCATAACGATTAT‑CCGGGAACCATTAACGAATATGCCACACCGCAACGAAGCTCGGCTTATTGCGGGGGGTTATCTAAtttttttcggagcctgaaaaat > 2:185571/1‑233 (MQ=255) gcctggtctttcgctacaaacaCCGCCAATGGTACAGCTAGCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAG‑CCGGAAACCATTACAGACTATGCCACACTGCAACTATGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAg < 2:97301/230‑1 (MQ=255) gTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCCATGTTTTTGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAAACTGGGCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAAACATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATTGCGGCGTGTTTTCTAATTTTTTCGGGACCGTAAAATATAGTGGCAGGAGCTAtt > 1:186385/1‑250 (MQ=255) aaaaCCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTaccac < 1:167231/251‑1 (MQ=255) aCCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCCATGTTTATGGAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAAACTGGTCGGGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAAACATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTTCTGGACCGCAAAATATAGTGGCAGTAGCTCTTCATTTTCTtccaccc > 1:205022/1‑249 (MQ=255) gCCAATGGTACCGCTATCCCTATTGGCGGTGGGAACGCCAACGTTTTTTTAAACCTTCCGCCCGTCGTGAATGTGGGGCAAAAACTGGGCGTGGCTCTTTTGCCGCGAAACTTTTTGCC‑TAAAGATTAT‑CCGGGAACCATTTCAAACCATTACAAACAGCAACGAGGCTCGGCTTATGGCGGCGTGTTTTTTAATTTTTTCGGGGCCGTGAAACATATTGGCAGGAGcgttcccttttttccccccccacc > 2:103635/1‑228 (MQ=255) aTTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTtata < 1:70707/250‑1 (MQ=255) ggCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTTGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAAtt < 1:195933/250‑1 (MQ=255) gTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCGACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGt < 1:277627/250‑1 (MQ=255) aTGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGcc < 1:79411/250‑1 (MQ=255) aaaCCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑ACATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAACATATAGTGGCAGTAGCTATCCATTTCCTACCACCACCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTgg > 2:121160/1‑251 (MQ=255) aaaCCGTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACTGATAAGCCGTGGCCGGTgg < 2:65245/251‑1 (MQ=255) ggTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCgggg < 1:227898/251‑1 (MQ=255) gATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGAAGCCTGTGAGCAGTGCGGGCGGGGTGGCGAt > 1:22809/1‑251 (MQ=255) gC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCCGCGAAAAGCCGCGCGTTGTTTATAATTCGAGAACGGAGCAGCCGAGGACGGTGGCGCTTTATTTGACGCCTGTGAGCCGTGCGGGCGGGGGGGCGATTAAAGCTGGcc > 2:92036/1‑250 (MQ=255) aTAACGATTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTg < 1:53329/251‑1 (MQ=255) aTTAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTCTTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTAtt > 1:40957/1‑251 (MQ=255) ttAT‑CCGGAAACCATTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGGGGCGATTAAAGCTGGCTCATTAATTGCAGGGCTTAttt > 2:23875/1‑251 (MQ=255) gaTTACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGAGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGGAGGGTGGCGATTTAAGCTGGCTCATTCATTGCCCTGCTTATTTTGCGACAGAcc > 2:150949/2‑250 (MQ=255) tACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCtg < 2:209711/179‑1 (MQ=255) tACAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGGTGTTTATAATTCGAGAACGGAAAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCtg > 1:209711/1‑179 (MQ=255) aCAGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTGTAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACcaacaa < 2:233120/251‑1 (MQ=255) aGACTATGCCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACt < 1:197725/251‑1 (MQ=255) | TGTAATGAAACGAGTTATTACCCTGTTTGCTGTACTGCTGATGGGCTGGTCGGTAAATGCCTGGTCATTCGCCTGTAAAACCGCCAATGGTACCGCTATCCCTATTGGCGGTGGCAGCGCCAATGTTTATGTAAACCTTGCGCCCGTCGTGAATGTGGGGCAAAACCTGGTCGTGGATCTTTCGACGC‑AAATCTTTTGCCATAACGATTAT‑CCGGAAACCATTACAGACTATGTCACACTGCAACGAGGCTCGGCTTATGGCGGCGTGTTATCTAATTTTTCCGGGACCGTAAAATATAGTGGCAGTAGCTATCCATTTCCTACCACCAGCGAAACGCCGCGCGTTGTTTATAATTCGAGAACGGATAAGCCGTGGCCGGTGGCGCTTTATTTGACGCCTGTGAGCAGTGCGGGCGGGGTGGCGATTAAAGCTGGCTCATTAATTGCCGTGCTTATTTTGCGACAGACCAACAACT > NC_000913/4548804‑4549279 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |