Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,415,251	IS30 (–) +2 bp	coding (505‑506/2145 nt)	pta →	phosphate acetyltransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 271761	NA (NA)	22 (0.790)	21/488	0.4	100%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	= 2415252	0 (0.000)					coding (506/2145 nt)	pta	phosphate acetyltransferase
*	?	NC_000913	1469296 =	NA (NA)	31 (1.120)	29/488	0.1	100%	noncoding (1/1221 nt)	IS30	repeat region
	?	NC_000913	2415251 =	0 (0.000)					coding (505/2145 nt)	pta	phosphate acetyltransferase

TTACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGAT  >  NC_000913/271513‑272010                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          ttACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACa                                                                                                                                                                                                                                                           >  1:137678/1‑249 (MQ=31)      gTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGGCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggt                                                                                                                                                                                                                                                      <  1:43740‑M1/249‑6 (MQ=255)         aGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatt                                                                                                                                                                                                                                                 <  1:263002‑M1/251‑11 (MQ=255)         aGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGctct                                                                                                                                                                                                                                                 <  1:130009/251‑1 (MQ=35)           cACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttc                                                                                                                                                                                                                                               <  2:274458‑M1/251‑13 (MQ=255)                    tAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGAc                                                                                                                                                                                                                                      >  1:31922/1‑251 (MQ=40)                    tAAAGTTTACTTCTGCGATCCTCAGAGGCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGa                                                                                                                                                                                                                                       <  2:161944/250‑1 (MQ=255)                                                ccgtggcAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggc                                                                                                                                                                                                           <  2:180475‑M1/247‑49 (MQ=255)                                                 cTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGAAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGCTCTGGTTGCTGCTCAGCTAAACAACAGAGCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttc                                                                                                                                                                                                         >  1:164575‑M1/1‑201 (MQ=255)                                                     gCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCa                                                                                                                                                                                                     <  2:202990/251‑1 (MQ=255)                                                     gCAGCGGGGAACAAATGAGAATACAAAGGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgata                                                                                                                                                                                                     <  2:261211‑M1/251‑55 (MQ=255)                                                          ggggAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatc                                                                                                                                                                                                 >  1:8673‑M1/1‑192 (MQ=255)                                                              aaCAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGtt                                                                                                                                                                                            <  1:84238/251‑1 (MQ=255)                                                                 aaaTGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCCATATACTCAACATGAATTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgtt                                                                                                                                                                                         >  2:108293‑M1/1‑185 (MQ=255)                                                                        aaTACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGCGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGc                                                                                                                                                                                  >  2:233410/1‑251 (MQ=255)                                                                        aaTACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGc                                                                                                                                                                                  <  1:195994/251‑1 (MQ=255)                                                                           aCAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaag                                                                                                                                                                               <  2:40836‑M1/251‑77 (MQ=255)                                                                              aaTGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgt                                                                                                                                                                            >  1:221192‑M1/1‑172 (MQ=255)                                                                                     tacttcggCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCCACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgctggtcagttcgatacgctctttcagctgttcctgggtgtcagtgcc                                                                                                                                                                     >  2:157419‑M1/4‑165 (MQ=255)                                                                                           ggCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTc                                                                                                                                                               >  1:199493/1‑251 (MQ=255)                                                                                            gCAGTACTTTCCTAAAAAGACATGTCTTGCCCACTATACTCAACATGCACTATATCTGGTTGCTGCTCAGCTAAAAAACAGACCGAGAAAAACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgagggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagac                                                                                                                                                              >  2:45033‑M1/1‑158 (MQ=255)                                                                                              aGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgata                                                                                                                                                             >  2:247277‑M1/1‑156 (MQ=255)                                                                                               gTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacata                                                                                                                                                           <  1:61211‑M1/251‑97 (MQ=255)                                                                                                 aCTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataac                                                                                                                                                         >  2:195600‑M1/1‑153 (MQ=255)                                                                                                   tttCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgctacttgac                                                                                                                                                       >  2:104331‑M1/1‑151 (MQ=255)                                                                                                     tCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacga                                                                                                                                                       >  1:257981‑M1/1‑149 (MQ=255)                                                                                                     tCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgact                                                                                                                                                      <  1:148966‑M1/250‑102 (MQ=255)                                                                                                      ccTAAAAAGACATGTCTAGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaag                                                                                                                                                     <  1:110121‑M1/250‑103 (MQ=255)                                                                                                              gACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGCTGCTGCTAAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCt                                                                                                                                            >  1:182692/1‑251 (MQ=255)                                                                                                                cATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttca                                                                                                                                          <  2:12905‑M1/251‑114 (MQ=255)                                                                                                                        gCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACt                                                                                                                                   <  1:242202/250‑1 (MQ=255)                                                                                                                        gCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTg                                                                                                                                  <  1:88989/251‑1 (MQ=255)                                                                                                                            aaTATACTCAACATGAACTAGATCTGGTTGATGCCCCGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGCAAGAGATAATTGACAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatat                                                                                                                              >  2:172271‑M1/1‑126 (MQ=255)                                                                                                                              tataCTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTGAAAACACCGTACGAGCTAATTGAAAGGGGAGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGCAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTGGGGGTTCATCGCAGCAGCTACAAATACTGGaaaa                                                                                                                             >  1:34227/1‑250 (MQ=37)                                                                                                                                  cTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgat                                                                                                                        >  1:61520‑M1/1‑120 (MQ=255)                                                                                                                                    cAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactg                                                                                                                        <  2:31087‑M1/249‑132 (MQ=255)                                                                                                                                    cAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAAAAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgtggcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagagattt                                                                                                                      >  1:170333‑M1/1‑118 (MQ=255)                                                                                                                                       cATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTg                                                                                                                    >  2:65531/1‑250 (MQ=255)                                                                                                                                                                    aaaCAACAGACCGAGAAAGACACTGAAGTTCAAAACATCGAAAGAGATAATTGAACGGGGAGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcaccacgctgttaatgaagtccgccgtgcgggtgataattgactgcaccagggtcaggcgttacatattgactgccccgaccgcgccgcattcgtcaatgttgatggc                                                                                      >  2:40249‑M1/1‑86 (MQ=255)                                                                                                                                                                    aaaCAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatg                                                                                        <  2:216487‑M1/249‑164 (MQ=255)                                                                                                                                                                     aacaacAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGTAAAAACCTTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACg                                                                                     <  1:162675/251‑1 (MQ=255)                                                                                                                                                                        aacaGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtg                                                                                  <  2:221192‑M1/251‑170 (MQ=255)                                                                                                                                                                        aacaGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGc                                                                                    >  2:229689/1‑249 (MQ=255)                                                                                                                                                                                gagaAAGACCCTGAAGTTCAAAACACAGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgcccagaccgcgccgctttcgtcaatgttgatggcgcaaggcatcac                                                                          >  1:68116‑M1/1‑74 (MQ=255)                                                                                                                                                                                   aaaGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCGCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCATACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTg                                                                       <  2:217003/251‑1 (MQ=255)                                                                                                                                                                                     aGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccg                                                                      >  1:244667‑M1/1‑69 (MQ=255)                                                                                                                                                                                      gACACTGAAGTTCAAAACACCGAAAGAGATAAGTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagacct                                                                    <  1:114092‑M1/251‑184 (MQ=255)                                                                                                                                                                                       acacTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagacctt                                                                   <  2:61520‑M1/251‑185 (MQ=255)                                                                                                                                                                                       acacTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTc                                                                                >  2:11690/1‑238 (MQ=255)                                                                                                                                                                                       acacTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTc                                                                                <  1:11690/238‑1 (MQ=255)                                                                                                                                                                                          cTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccgacaatc                                                                >  1:90987‑M1/1‑64 (MQ=255)                                                                                                                                                                                               gTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCa                                                           <  1:220322/251‑1 (MQ=255)                                                                                                                                                                                                 tCAAAACACCGAAAGAGATAATTGAACGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCCGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATc                                                         >  2:263770/1‑251 (MQ=255)                                                                                                                                                                                                  cAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcttcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaat                                                                                               <  2:102154‑M1/212‑157 (MQ=255)                                                                                                                                                                                                  cAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaat                                                                                               >  1:102154‑M1/1‑56 (MQ=255)                                                                                                                                                                                                    aaaCACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccgacaatccagtttggat                                                      >  2:72656‑M1/1‑54 (MQ=255)                                                                                                                                                                                                         ccGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACgg                                                 <  1:65531/251‑1 (MQ=255)                                                                                                                                                                                                              agagATAATTGAAAGGGGTGTTGCATTGACATATTGAATCTACAagtgcctatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccgacaatccagtttggatgcgggttttt                                            <  1:45875‑M1/251‑208 (MQ=255)                                                                                                                                                                                                              agagATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtct                                            >  1:157052‑M1/1‑44 (MQ=255)                                                                                                                                                                                                              agagATAATGGAAAGGGGTTTTGCATTGACAGATTGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccgacaatccagtttggatgcgggtttgt                                            <  1:45877‑M1/251‑208 (MQ=255)                                                                                                                                                                                                                       tGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGCAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCa                                    >  1:127133/1‑250 (MQ=255)                                                                                                                                                                                                                        gAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCaag                                  <  2:50178/251‑1 (MQ=255)                                                                                                                                                                                                                         aaaGGGGTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtctttggtgttagc                                 <  1:129326‑M1/251‑219 (MQ=255)                                                                                                                                                                                                                            ggggTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGCCGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGc                              >  2:149000/1‑251 (MQ=255)                                                                                                                                                                                                                              ggTGTTGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtcgttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtctttggtgttagcgtggt                            >  1:244791‑M1/1‑28 (MQ=255)                                                                                                                                                                                                                                tgtTGCATTGACAGATTGAATCTACAccggcgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtctttggtgttagcgtggt                            >  1:214056‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                tgtTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCg                          >  1:212397/1‑251 (MQ=255)                                                                                                                                                                                                                                  ttGCATTGACAGATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtctttggtgttagcgtggtagtt                        >  1:57952‑M1/1‑24 (MQ=255)                                                                                                                                                                                                                                      aTTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCcaca                     >  1:69399/1‑250 (MQ=255)                                                                                                                                                                                                                                             gATTGAATCTACAccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtctttggtgttagcgtggtagtttgcgacgatct             >  1:241634‑M1/1‑13 (MQ=255)                                                                                                                                                                                                                                               ttGAATCTACAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccgacaatccagtttggatgcgggtttttaccgccaaagaccgcgtggcttttgacaatttc           >  2:259084‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                                      tACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAgg    >  2:63555/1‑251 (MQ=255)                                                                                                                                                                                                                                                       acaccggtgatgttggtgtttttggcaccgccgaagctgttgcgggtcagttcgatacgctctttcagctgttccggggtgtcagtgccctgagacataacgaagacgatttccgcattcagcgttttagcgatttcgtagttcagagactgggcaaactggtgcttacgtgtcgggaccagaccttcaaccagaacgacttcagcgtctttggtgttagcgtggtagtttgcgacgatctcttccatcag   <  2:157052‑M1/251‑249 (MQ=255)                                                                                                                                                                                                                                                        cAagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgctaatgaagtccccgtgcgggtgataattgactgcaccaggttccggcggtccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcctcccgccgacaatcccgtttggatgcgggtttttaccgccaaagaccgcgtggattttgacaatttcacgctggaa   >  2:150458‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                                         aagtgccgatttcgctccacggtttgttgaactgaccgatggctaaggcgtcggggatcatcacgttgttaatgaagtccgccgtgcgggtgataattgactgcaccaggttcaggcgttccatattgactgccccgaccgcgccgctttcgtcaatgttgatggcgcaaggcatcccgccgacaatccagtttggatgcgggtttttaccgccaaagaccgcgtggattttgacaatttcacgctggaa  >  1:53138‑M1/1‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                          TTACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGAT  >  NC_000913/271513‑272010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.