Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	791,653	IS1 (–) +8 bp	coding (396‑403/1017 nt)	galE ←	UDP‑glucose 4‑epimerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	30 (1.090)	29/484	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	= 791660	0 (0.000)					coding (396/1017 nt)	galE	UDP‑glucose 4‑epimerase
*	?	NC_000913	= 258675	NA (NA)	35 (1.260)	32/488	0.1	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	791653 =	0 (0.000)					coding (403/1017 nt)	galE	UDP‑glucose 4‑epimerase

AAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGG  >  NC_000913/257902‑258152       |                                                                                                                                                                                                                                                     ctccaaggtaATGAATCCAACTTATTGAAAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAgg  >  2:231427/6‑251 (MQ=2)       |                                                                                                                                                                                                                                                     AAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGG  >  NC_000913/257902‑258152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.