Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 258,921 IS1 (+) +9 bp pseudogene (246‑254/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)34 (1.220) 33/488 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 258921 = 0 (0.000)pseudogene (246/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 = 258675NA (NA)23 (0.830) 23/488 0.3 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 258929 0 (0.000)pseudogene (254/331 nt) crl RNA polymerase holoenzyme assembly factor Crl

AAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGG  >  NC_000913/257902‑258152
      |                                                                                                                                                                                                                                                    
ctccaaggtaATGAATCCAACTTATTGAAAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAgg  >  2:231427/6‑251 (MQ=2)
      |                                                                                                                                                                                                                                                    
AAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGG  >  NC_000913/257902‑258152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.